##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2257506 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.094640722992544 33.0 31.0 34.0 30.0 34.0 2 32.203324376546504 34.0 31.0 34.0 30.0 34.0 3 32.26461192129722 34.0 31.0 34.0 30.0 34.0 4 35.70642647239919 37.0 35.0 37.0 35.0 37.0 5 35.748345740830814 37.0 35.0 37.0 35.0 37.0 6 35.830389376595235 37.0 36.0 37.0 35.0 37.0 7 35.7545220256336 37.0 36.0 37.0 35.0 37.0 8 35.74446136577267 37.0 36.0 37.0 35.0 37.0 9 37.42794659239001 39.0 38.0 39.0 35.0 39.0 10-11 37.3384374172206 39.0 37.5 39.0 34.5 39.0 12-13 37.24087997994247 39.0 37.0 39.0 34.0 39.0 14-15 38.56086871972876 40.0 38.0 41.0 34.0 41.0 16-17 38.49416435659529 40.0 38.0 41.0 34.0 41.0 18-19 38.41513067960838 40.0 38.0 41.0 34.0 41.0 20-21 38.31360891178141 40.0 38.0 41.0 34.0 41.0 22-23 38.226984114327934 40.0 38.0 41.0 34.0 41.0 24-25 38.26832951938998 40.0 38.0 41.0 34.0 41.0 26-27 38.18893858975347 40.0 38.0 41.0 34.0 41.0 28-29 38.06613182866403 40.0 38.0 41.0 33.0 41.0 30-31 37.909530251525354 40.0 38.0 41.0 33.0 41.0 32-33 37.71922710282941 40.0 38.0 41.0 33.0 41.0 34-35 37.40641110145444 40.0 37.0 41.0 31.5 41.0 36-37 37.15191166712292 40.0 37.0 41.0 31.0 41.0 38-39 36.919660678642714 40.0 36.5 41.0 30.5 41.0 40-41 36.77908652291511 40.0 36.5 41.0 30.0 41.0 42-43 36.589306739384085 40.0 36.0 41.0 30.0 41.0 44-45 36.336124023590635 39.5 36.0 41.0 30.0 41.0 46-47 35.98085785818509 39.0 35.0 41.0 28.5 41.0 48-49 35.97667403763268 39.0 35.5 41.0 28.0 41.0 50-51 34.984609343230986 38.0 34.0 40.0 26.5 40.5 52-53 35.156810214457906 38.5 35.0 40.0 27.5 40.5 54-55 35.66900907461597 39.0 35.0 41.0 28.0 41.0 56-57 35.54508581594025 39.0 35.0 41.0 27.5 41.0 58-59 35.35235742452069 39.0 35.0 41.0 27.0 41.0 60-61 35.061761076161034 38.5 35.0 40.5 26.0 41.0 62-63 34.698699804120125 38.0 34.5 40.0 26.0 41.0 64-65 34.32786623822926 37.5 34.0 40.0 25.5 41.0 66-67 33.934961856136816 37.0 34.0 40.0 24.5 41.0 68-69 33.53921695003247 36.0 34.0 39.0 23.0 41.0 70-71 33.12373101112466 36.0 33.0 39.0 22.5 41.0 72-73 32.67058980131171 35.0 33.0 38.5 21.5 40.0 74-75 32.214943614767805 35.0 32.5 37.0 20.0 39.5 76-77 31.778645328074433 35.0 32.0 37.0 19.0 39.0 78-79 31.348587999323147 35.0 32.0 36.0 18.0 39.0 80-81 30.919211067434595 35.0 31.0 36.0 13.5 37.5 82-83 30.500042746287274 35.0 31.0 35.5 8.5 37.0 84-85 30.123485386085353 34.0 31.0 35.0 6.5 37.0 86-87 29.75008017697406 34.0 30.0 35.0 2.0 36.0 88-89 29.36345684131072 34.0 30.0 35.0 2.0 36.0 90-91 28.988975223100184 34.0 29.5 35.0 2.0 35.5 92-93 28.578909867792156 34.0 29.0 35.0 2.0 35.0 94-95 28.11707167112734 34.0 29.0 35.0 2.0 35.0 96-97 27.55485079552391 34.0 27.5 35.0 2.0 35.0 98-99 26.856869483403365 34.0 26.0 35.0 2.0 35.0 100-101 24.301482255196664 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19001663604450414 1101 2 0.2667798935376524 1101 3 0.22146299763728905 1101 4 0.03900410445264413 1101 5 0.13092028736750194 1101 6 0.25445636737194377 1101 7 0.10896054708679515 1101 8 0.1647441771051703 1101 9 0.2590255447740759 1101 10-11 0.28630385829767135 1101 12-13 0.21689646566750298 1101 14-15 0.2700225964477241 1101 16-17 0.4361440422022653 1101 18-19 0.42933392213506494 1101 20-21 0.4364110071200642 1101 22-23 0.4344151952088353 1101 24-25 0.3341239475933122 1101 26-27 0.33106868183693194 1101 28-29 0.31210946788064575 1101 30-31 0.3039538912857296 1101 32-33 0.27002595805600293 1101 34-35 0.2914976842431187 1101 36-37 0.08691353223394316 1101 38-39 0.13588555075797615 1101 40-41 0.10557589241950893 1101 42-43 0.10138683682320249 1101 44-45 0.034972676759842614 1101 46-47 0.04107752833734679 1101 48-49 -0.006015306925988284 1101 50-51 -0.1009889749271835 1101 52-53 0.05893229865606742 1101 54-55 0.03821135514369445 1101 56-57 0.1564053213484513 1101 58-59 0.2576957441693324 1101 60-61 0.11636500737908761 1101 62-63 0.1571402360297398 1101 64-65 0.2527153291715365 1101 66-67 0.06937348343461736 1101 68-69 0.06865765506572075 1101 70-71 0.00660252473342382 1101 72-73 -0.11182073602802944 1101 74-75 -0.1631843205053869 1101 76-77 -0.17293559184290075 1101 78-79 -0.10195496442312191 1101 80-81 -0.22083342425939634 1101 82-83 -0.15462032546388826 1101 84-85 -0.19205873964400055 1101 86-87 -0.3144148286897135 1101 88-89 -0.2510515763825403 1101 90-91 -0.2582066349753056 1101 92-93 -0.4708830513343827 1101 94-95 -0.5247222949217019 1101 96-97 -0.4639154219373758 1101 98-99 -0.6875583352325059 1101 100-101 -0.7384490805267312 1102 1 0.15693861461290481 1102 2 0.17701092115665062 1102 3 0.2880113601589045 1102 4 0.20525006657605616 1102 5 0.08670221237969855 1102 6 0.2216110006214862 1102 7 0.1852690015754277 1102 8 0.21868204766096966 1102 9 0.2610662152453358 1102 10-11 0.2540230212882477 1102 12-13 0.2998667512549247 1102 14-15 0.397859247517907 1102 16-17 0.4196452011672491 1102 18-19 0.45269189456218584 1102 20-21 0.47726192514299015 1102 22-23 0.44744892608261466 1102 24-25 0.4670464350621515 1102 26-27 0.4169285233580311 1102 28-29 0.35646642605501455 1102 30-31 0.36439342109011363 1102 32-33 0.35182777479000293 1102 34-35 0.2828045598225444 1102 36-37 0.15599410526394308 1102 38-39 0.27475150364615786 1102 40-41 0.25292613807548037 1102 42-43 0.2974354074161525 1102 44-45 0.11643412831216438 1102 46-47 0.11742894030796691 1102 48-49 0.083587664565961 1102 50-51 0.04757079629064975 1102 52-53 0.03732531881288992 1102 54-55 0.07495254733927226 1102 56-57 0.16068738397665783 1102 58-59 0.1844695840512074 1102 60-61 0.12515241016719614 1102 62-63 0.08370663815941981 1102 64-65 0.0340612390363475 1102 66-67 -0.02429448234740761 1102 68-69 -0.1290494037989518 1102 70-71 -0.11947020849275702 1102 72-73 -0.08182893723225249 1102 74-75 -0.11840019515773292 1102 76-77 -0.05900918168435609 1102 78-79 -0.0593183330196041 1102 80-81 -0.17985135286228981 1102 82-83 -0.1482856808502504 1102 84-85 -0.044854219959336916 1102 86-87 -0.2202434113563818 1102 88-89 -0.1363047923646512 1102 90-91 -0.2995776046384542 1102 92-93 -0.2305346724914621 1102 94-95 -0.2685484557494462 1102 96-97 -0.4260450435415173 1102 98-99 -0.5578082387999324 1102 100-101 -0.6173535861710171 1103 1 0.2991465200445873 1103 2 0.3646490530986455 1103 3 0.3286718897808001 1103 4 0.2770001874143375 1103 5 0.3313941455488063 1103 6 0.34576991401583257 1103 7 0.3033899749780957 1103 8 0.2854689813293234 1103 9 0.3052870654003996 1103 10-11 0.3378321320724851 1103 12-13 0.3487552129252265 1103 14-15 0.5568782045978438 1103 16-17 0.5118434445040023 1103 18-19 0.5059902704857393 1103 20-21 0.4946267464737204 1103 22-23 0.38623471122841835 1103 24-25 0.42904670728981387 1103 26-27 0.40279044432160305 1103 28-29 0.38859922546445347 1103 30-31 0.44032022463563436 1103 32-33 0.5086582301106475 1103 34-35 0.4052979416600877 1103 36-37 0.4328365972200672 1103 38-39 0.5258699718588673 1103 40-41 0.3347211118984106 1103 42-43 0.37541571661312645 1103 44-45 0.4743584867763815 1103 46-47 0.44476270880814894 1103 48-49 0.5666852080093747 1103 50-51 0.5762704472133109 1103 52-53 0.5780747695140889 1103 54-55 0.5830716876761031 1103 56-57 0.5536314963331606 1103 58-59 0.47165026920054487 1103 60-61 0.5118608697411346 1103 62-63 0.38720918482932376 1103 64-65 0.4445355957370438 1103 66-67 0.39741783461302305 1103 68-69 0.18623270205090847 1103 70-71 0.38852846613606573 1103 72-73 0.13857735758533352 1103 74-75 0.11745874521099609 1103 76-77 0.11385054009698692 1103 78-79 0.2065447652789203 1103 80-81 0.27990266858110147 1103 82-83 0.2875962597753059 1103 84-85 0.19497623883865955 1103 86-87 0.08635945071013396 1103 88-89 0.19158237324365857 1103 90-91 0.1938000103988493 1103 92-93 0.20335846622889875 1103 94-95 0.12545687256059423 1103 96-97 -0.10991967773559708 1103 98-99 -0.15834308170519407 1103 100-101 -0.11501394341517823 1104 1 0.26524797284984203 1104 2 0.2902169342052048 1104 3 0.3451356117765414 1104 4 0.2715936290418597 1104 5 0.32841909788945145 1104 6 0.20351261597309644 1104 7 0.2962767832274622 1104 8 0.26643405115142826 1104 9 0.3214036016585524 1104 10-11 0.33134406833751484 1104 12-13 0.24903954690725527 1104 14-15 0.3861616025826109 1104 16-17 0.43014390979100625 1104 18-19 0.3348384757485334 1104 20-21 0.3993798257497261 1104 22-23 0.44083269483950716 1104 24-25 0.4475358004765013 1104 26-27 0.4420724283839874 1104 28-29 0.45416600254942097 1104 30-31 0.3196742703179112 1104 32-33 0.39539336225670496 1104 34-35 0.31411604780789304 1104 36-37 0.3818347944690146 1104 38-39 0.4893364204974091 1104 40-41 0.36186382170004094 1104 42-43 0.20963452691365347 1104 44-45 0.2726722632710832 1104 46-47 0.37612730854947785 1104 48-49 0.47680235535315774 1104 50-51 0.46927162822588997 1104 52-53 0.6615814132545523 1104 54-55 0.5946593143056873 1104 56-57 0.5194604543353094 1104 58-59 0.6262414979426794 1104 60-61 0.5307702658849038 1104 62-63 0.6536640698101266 1104 64-65 0.5666493419195788 1104 66-67 0.38869473242856145 1104 68-69 0.527479481382656 1104 70-71 0.49910300768868154 1104 72-73 0.5313223862196779 1104 74-75 0.5683808695616008 1104 76-77 0.6142092260594971 1104 78-79 0.5146440226307121 1104 80-81 0.43774870308565283 1104 82-83 0.3234399186965504 1104 84-85 0.45772236181020176 1104 86-87 0.39638711419853934 1104 88-89 0.5064509050934696 1104 90-91 0.45238656254425536 1104 92-93 0.6464633429537514 1104 94-95 0.4696818469816719 1104 96-97 0.2895114606123492 1104 98-99 0.23917599293102398 1104 100-101 0.3058844832087857 1105 1 0.16211986237501463 1105 2 0.2221615915289803 1105 3 0.22441479414754895 1105 4 0.2721312391261179 1105 5 0.2655778112816449 1105 6 0.26624518023239574 1105 7 0.2749955953792451 1105 8 0.2288839834711851 1105 9 0.2869305380243503 1105 10-11 0.2691159896253055 1105 12-13 0.36863355318960345 1105 14-15 0.3476089117160299 1105 16-17 0.4307580410186418 1105 18-19 0.3914254573206719 1105 20-21 0.435730715934163 1105 22-23 0.45490346731757114 1105 24-25 0.3924566201368265 1105 26-27 0.4480671616979848 1105 28-29 0.5185556015832518 1105 30-31 0.3444365353991614 1105 32-33 0.35117014304105254 1105 34-35 0.44867565073817417 1105 36-37 0.480344975128169 1105 38-39 0.6042981853173472 1105 40-41 0.4956402082216087 1105 42-43 0.4494959947900057 1105 44-45 0.38514253197917725 1105 46-47 0.35471062172339174 1105 48-49 0.41149346385021346 1105 50-51 0.28548169180653105 1105 52-53 0.4464937521100296 1105 54-55 0.3544969555985773 1105 56-57 0.29764020215486653 1105 58-59 0.3388459729637745 1105 60-61 0.3829920105392759 1105 62-63 0.319147447986623 1105 64-65 0.25344276207074756 1105 66-67 0.2722235505450712 1105 68-69 0.3428029153653327 1105 70-71 0.3171276495743456 1105 72-73 0.344455445754285 1105 74-75 0.2713791133840431 1105 76-77 0.3571550846382614 1105 78-79 0.10290914173131327 1105 80-81 0.06950368492370984 1105 82-83 0.019119371049846734 1105 84-85 0.25757432859979446 1105 86-87 0.18712440868791447 1105 88-89 0.18477863995682853 1105 90-91 0.21401408300991065 1105 92-93 0.33966745750601746 1105 94-95 0.011943562680350794 1105 96-97 -0.04168474375427067 1105 98-99 0.16023872041695597 1105 100-101 0.25705118249985404 1106 1 -0.018369681351224187 1106 2 0.024451827610931787 1106 3 0.05787699833572901 1106 4 0.06648527692193085 1106 5 -0.045166471108686324 1106 6 -0.06504986545173352 1106 7 0.03450307225342897 1106 8 0.05939161640915813 1106 9 0.08715647582583586 1106 10-11 0.03220051944767022 1106 12-13 0.10857719288087964 1106 14-15 0.014506718310435929 1106 16-17 0.20107914226148438 1106 18-19 0.09188673221993326 1106 20-21 0.03662063421348449 1106 22-23 0.17833183403909203 1106 24-25 0.13456564488002698 1106 26-27 0.04120798089099509 1106 28-29 -0.028468368395756727 1106 30-31 -0.07070732703770943 1106 32-33 -0.07049470288142601 1106 34-35 0.07250783250992043 1106 36-37 -0.036826475130048664 1106 38-39 0.10456508986305835 1106 40-41 -0.005030156683901055 1106 42-43 0.012125456795565981 1106 44-45 -0.004828950910557239 1106 46-47 -0.07894276060451233 1106 48-49 -0.233265453067105 1106 50-51 -0.2233226112977178 1106 52-53 -0.20280786244079252 1106 54-55 -0.28293849074385236 1106 56-57 -0.3240079733175918 1106 58-59 -0.1989587061123288 1106 60-61 -0.19084212202981377 1106 62-63 -0.04930496674580098 1106 64-65 -0.19746098913520171 1106 66-67 -0.22080678258017628 1106 68-69 -0.10477649982109227 1106 70-71 -0.23244211093867762 1106 72-73 -0.1294029831090029 1106 74-75 -0.29597748527664436 1106 76-77 -0.24284988102769844 1106 78-79 -0.247997624343256 1106 80-81 -0.1982840399500212 1106 82-83 -0.16085409810627382 1106 84-85 -0.037711658290849215 1106 86-87 -0.1002395675336416 1106 88-89 -0.058929151882455955 1106 90-91 -0.16899127990933138 1106 92-93 -0.24540415449103037 1106 94-95 -0.28992547852049455 1106 96-97 -0.06513332783008963 1106 98-99 -0.15566896785272277 1106 100-101 -0.1842777006509344 1107 1 0.1409695635000361 1107 2 0.12960932103624856 1107 3 0.06349237112851824 1107 4 0.19304313408918716 1107 5 0.3033341607434963 1107 6 0.1917826766432853 1107 7 0.15404715675085612 1107 8 0.23493979086867256 1107 9 0.30644355017947333 1107 10-11 0.36887356155976647 1107 12-13 0.326866645218729 1107 14-15 0.3795155557024188 1107 16-17 0.31459149326390445 1107 18-19 0.35514292693287075 1107 20-21 0.2903410908547599 1107 22-23 0.3500289121496891 1107 24-25 0.4061708269217661 1107 26-27 0.3360386402372839 1107 28-29 0.33125158874076277 1107 30-31 0.36483649276487995 1107 32-33 0.3501632345481269 1107 34-35 0.30616068523713835 1107 36-37 0.23741519552127244 1107 38-39 0.3047884794938511 1107 40-41 0.10310109779883447 1107 42-43 0.18815712625148961 1107 44-45 0.2750909575562659 1107 46-47 0.36369253260905765 1107 48-49 0.29289034545743675 1107 50-51 0.24517859690610777 1107 52-53 0.28614689181993924 1107 54-55 0.36209890807440814 1107 56-57 0.2983220242916218 1107 58-59 0.2965826802055105 1107 60-61 0.27013353458414 1107 62-63 0.22190007782539567 1107 64-65 0.13485867683699837 1107 66-67 0.026976184734287756 1107 68-69 0.2564650256750056 1107 70-71 0.33724798264375977 1107 72-73 0.2824571598262082 1107 74-75 0.20371416658988295 1107 76-77 0.3825582613268885 1107 78-79 0.2957366860840125 1107 80-81 0.351522135124263 1107 82-83 0.4121295249682859 1107 84-85 0.4451767813758103 1107 86-87 0.37606288046663394 1107 88-89 0.42410049393878424 1107 90-91 0.40941175639655114 1107 92-93 0.4853210453108723 1107 94-95 0.403902542416013 1107 96-97 0.6022148568756727 1107 98-99 0.6423177504436666 1107 100-101 0.6579729312012468 1108 1 0.08108246533929275 1108 2 0.11749427414391533 1108 3 0.11252382235516478 1108 4 -0.013506646288490742 1108 5 0.10006889054458412 1108 6 0.15659459560612277 1108 7 0.13844250968167415 1108 8 0.24096251860657958 1108 9 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-0.9424727768073069 2316 58-59 -0.9046409859737636 2316 60-61 -0.7988011627859208 2316 62-63 -0.9535409501525507 2316 64-65 -1.1448693627399535 2316 66-67 -1.1373326774610675 2316 68-69 -1.1189795265926747 2316 70-71 -1.059152488135787 2316 72-73 -0.8606600662463251 2316 74-75 -0.9570219673790277 2316 76-77 -0.9839491839400729 2316 78-79 -0.7839886488054724 2316 80-81 -0.8574140703139612 2316 82-83 -0.8229456817354048 2316 84-85 -0.7369823624348832 2316 86-87 -0.5367165219449674 2316 88-89 -0.597335793459667 2316 90-91 -0.8811192147337756 2316 92-93 -1.1180219484011396 2316 94-95 -1.0014032347964203 2316 96-97 -1.0087007699889234 2316 98-99 -1.0215537719839283 2316 100-101 -0.6090293899123616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7447.0 3 1903.0 4 1939.0 5 2200.0 6 2522.0 7 2859.0 8 3455.0 9 4312.0 10 5188.0 11 6514.0 12 8600.0 13 12474.0 14 10962.0 15 11103.0 16 13031.0 17 17358.0 18 16597.0 19 11634.0 20 11588.0 21 12367.0 22 13027.0 23 14601.0 24 16114.0 25 18833.0 26 21475.0 27 25083.0 28 29854.0 29 35508.0 30 43068.0 31 53180.0 32 67094.0 33 88238.0 34 123347.0 35 184070.0 36 299609.0 37 489441.0 38 486836.0 39 84005.0 40 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58481971241981 18.544766916056894 14.305414515888906 23.56499885563439 2 15.318504301211647 19.798700279297154 40.57152588132931 24.311269538161888 3 19.802942165090812 23.568007080920626 29.385155431706455 27.243895322282107 4 13.138123854999156 16.04471307293281 36.419619222222074 34.39754384984596 5 14.360421250969718 36.64961501834313 33.806618961084006 15.18334476960315 6 32.895079845009356 34.885530364157084 17.26908787827737 14.950301912556194 7 29.23592944994724 32.13330554841227 20.39761995548501 18.23314504615548 8 26.113053445099442 35.093967334795074 19.531948007032305 19.261031213073178 9 26.207708639888843 17.598499361359618 20.51398912826142 35.679802870490114 10-11 25.517988791447415 26.076909060520066 26.850472978574647 21.55462916945787 12-13 26.302881953111456 23.363203768179297 27.551330758020658 22.782583520688586 14-15 22.87700249060895 24.912296647376547 26.01955909460213 26.191141767412375 16-17 22.686265973792917 28.20145265870057 26.52214852335789 22.590132844148624 18-19 22.48087346893532 26.973895693620797 28.502421991908218 22.042808845535664 20-21 23.195568747011023 26.038378148635346 28.464898443061887 22.30115466129174 22-23 22.836022834559422 25.972448817141263 28.470206026333443 22.72132232196587 24-25 22.936704706231954 26.141386544372345 28.458377500920314 22.463531248475388 26-27 22.666051948967787 26.238821374169042 28.625332001888502 22.46979467497467 28-29 22.259332912308587 26.760764514709845 28.58257613925039 22.397326433731177 30-31 22.50571444852495 26.21318257699068 28.470314911623106 22.810788062861263 32-33 22.396847782732557 26.39075158042094 28.509390293301028 22.703010343545476 34-35 23.152319258114705 26.331519770764984 28.143882481501088 22.372278489619234 36-37 23.02508463803761 26.040008038193434 28.351567258559886 22.583340065209065 38-39 22.568559217975164 26.46322071973357 28.445893165959575 22.522326896331695 40-41 22.78258660674525 26.373667438817744 28.353974694739332 22.489771259697676 42-43 22.691735075071055 26.450527779193177 27.8867538203052 22.970983325430574 44-45 22.794020128726295 26.40417759635557 27.906800276643146 22.895001998274985 46-47 22.94962597467568 26.678151286311525 27.621571026534504 22.750651712478295 48-49 23.101246101758893 26.668821104229373 27.524163975845372 22.705768818166362 50-51 22.638980469774893 26.893343175481387 27.67087138839046 22.79680496635325 52-53 23.04488631760922 26.993725903525974 27.224687074097186 22.736700704767618 54-55 22.648846616595893 26.553352118217045 27.493263854630545 23.304537410556517 56-57 22.875806815100102 26.968786797920973 27.325361641596896 22.830044745382025 58-59 23.199710846053325 26.550493507937833 27.325426085022382 22.92436956098646 60-61 22.995215740786442 26.537653084955622 27.910542934935588 22.556588239322352 62-63 22.69489218304253 26.693524307819093 27.84843105957822 22.763152449560156 64-65 22.508253704429915 26.866659218265028 27.575451789330117 23.04963528797494 66-67 22.583479084989726 26.889455622571397 27.29822057593457 23.22884471650431 68-69 22.42290692021249 26.716051174560885 27.664176738868342 23.196865166358283 70-71 22.656419203398332 26.74738384070193 27.452104691335162 23.144092264564584 72-73 22.72330386107046 26.467101053322722 27.65861882849146 23.150976257115357 74-75 22.453919179103647 27.024896219380544 27.330306166739003 23.190878434776803 76-77 22.61594218701807 26.710931974857033 27.305547235194815 23.367578602930084 78-79 22.6521167332022 26.75146637369229 27.391880596031985 23.204536297073528 80-81 22.65758687076999 26.946408749675438 27.329920140544456 23.066084239010117 82-83 22.682528623552745 26.738369167894803 27.42778662549714 23.15131558305531 84-85 22.54749293046713 26.726261100523995 27.247452787447607 23.47879318156127 86-87 22.547002221071494 26.65953383979668 27.459217491210623 23.334246447921203 88-89 22.48016727480718 26.627140315209296 27.2094195568642 23.683272853119323 90-91 22.500808000406003 26.568144038648683 27.366261464479592 23.56478649646572 92-93 22.741110876169916 26.420223243598162 27.42401816219104 23.414647718040886 94-95 22.530908164955136 26.278328403815642 27.469759590300992 23.721003840928233 96-97 22.36261684073601 26.27395744252729 27.60998287426541 23.753442842471287 98-99 22.387477698523206 26.323243213406883 27.233397195978792 24.055881892091122 100-101 22.48594807670524 25.634664904719312 27.765492166151517 24.11389485242393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2073.0 1 1186.0 2 342.0 3 413.0 4 371.5 5 336.0 6 580.0 7 1147.5 8 1853.5 9 2256.5 10 2219.5 11 2167.0 12 2296.0 13 2536.5 14 2782.0 15 2977.0 16 3143.5 17 3268.0 18 3436.5 19 3560.5 20 3763.5 21 4237.0 22 4903.0 23 5832.5 24 7218.0 25 9094.0 26 11289.0 27 13790.5 28 16301.5 29 18986.0 30 22413.5 31 26260.5 32 30213.0 33 33783.0 34 37537.0 35 41211.5 36 45399.5 37 49957.0 38 53373.0 39 55946.0 40 58266.0 41 63398.0 42 68992.0 43 73577.0 44 78603.5 45 83007.5 46 85147.5 47 90076.5 48 103061.5 49 104194.5 50 93894.0 51 88568.5 52 84881.5 53 82844.0 54 80454.5 55 73938.0 56 65754.5 57 58997.0 58 52909.5 59 45925.5 60 38502.5 61 31654.5 62 26298.0 63 21314.0 64 16723.5 65 13492.0 66 10628.0 67 8762.0 68 6596.0 69 4309.0 70 3194.0 71 2465.5 72 1970.5 73 1520.0 74 1172.0 75 976.0 76 704.5 77 409.0 78 256.0 79 181.5 80 111.5 81 77.0 82 62.0 83 38.0 84 30.5 85 30.0 86 23.0 87 12.5 88 12.0 89 12.5 90 10.5 91 8.0 92 6.0 93 10.0 94 9.0 95 5.5 96 5.5 97 2.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32562482668927567 2 0.11339947712209845 3 0.10772950326599354 4 0.11494986059837715 5 0.1321369688496952 6 0.08354352103604598 7 0.09240285518620991 8 0.09749697232255418 9 0.0856254645613345 10-11 0.08447375112181318 12-13 0.08801748478187876 14-15 0.09007727997179188 16-17 0.07884807393645908 18-19 0.0943962053699968 20-21 0.10376495123379517 22-23 0.11087456688930174 24-25 0.1256253582493247 26-27 0.12458438648668044 28-29 0.11915804431970503 30-31 0.10970070511440501 32-33 0.10327768785553615 34-35 0.3206635995651839 36-37 0.4754140188331726 38-39 0.45007632316370366 40-41 0.42848169617267906 42-43 0.46075182081465116 44-45 0.4681272164946627 46-47 0.4754361671685479 48-49 0.4863774448440004 50-51 0.4742623053936512 52-53 0.4787584174748594 54-55 0.46451703782847087 56-57 0.44347611922183156 58-59 0.4238526940792184 60-61 0.38418502542185934 62-63 0.45293345842713156 64-65 0.4845612813432168 66-67 0.49191452868785285 68-69 0.5045390798518364 70-71 0.49729657418407747 72-73 0.4891902834366775 74-75 0.49528107566491514 76-77 0.4728669602650004 78-79 0.45229115670124465 80-81 0.4538415401775233 82-83 0.4667318713660119 84-85 0.4668869097136398 86-87 0.47031990169682825 88-89 0.4829887495315627 90-91 0.4970086458241972 92-93 0.49085140858983317 94-95 0.49497099896965946 96-97 0.4951924823234135 98-99 0.4880607183325315 100-101 0.47616706223593647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2257506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.17923085136022 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46976680678668 42.19542915781365 2 13.2586583801219 14.632051430721363 3 4.390455125645267 7.267858107615538 4 1.9862717891833443 4.3840379835556815 5 1.0652071824130815 2.9388656511449196 6 0.6404968627518123 2.120527454961254 7 0.43295020598611766 1.672290155427636 8 0.29024376835482946 1.2812342317775884 9 0.2109517715288337 1.0476140849723625 >10 1.1183257458150975 11.225614546406264 >50 0.08299650843394467 3.1846708877055 >100 0.048778109641940494 4.930015198183839 >500 0.0032919258496953087 1.1342681131374739 >1k 0.0013649448645075624 1.2009897668512621 >5k 2.4087262314839334E-4 0.7845332297256216 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6513 0.28850421660008874 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5847 0.25900263388004285 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5348 0.2368985951753838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3208 0.14210371976862965 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3086 0.13669952593702964 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2382 0.10551466972845255 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2319 0.10272397947115092 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.429667075081971E-5 0.0 6 4.429667075081971E-5 0.0 0.0 4.429667075081971E-5 0.0 7 8.859334150163942E-5 0.0 0.0 4.429667075081971E-5 0.0 8 8.859334150163942E-5 0.0 0.0 4.429667075081971E-5 0.0 9 8.859334150163942E-5 0.0 0.0 4.429667075081971E-5 0.0 10-11 1.3289001225245912E-4 0.0 0.0 8.859334150163942E-5 0.0 12-13 1.3289001225245912E-4 0.0 0.0 8.859334150163942E-5 4.429667075081971E-5 14-15 1.7718668300327885E-4 0.0 0.0 8.859334150163942E-5 4.429667075081971E-5 16-17 1.7718668300327885E-4 0.0 0.0 8.859334150163942E-5 4.429667075081971E-5 18-19 1.7718668300327885E-4 0.0 0.0 1.1074167687704927E-4 4.429667075081971E-5 20-21 1.7718668300327885E-4 0.0 0.0 1.7718668300327885E-4 8.859334150163942E-5 22-23 1.7718668300327885E-4 0.0 0.0 2.2148335375409854E-4 8.859334150163942E-5 24-25 2.2148335375409854E-4 0.0 0.0 4.651150428836069E-4 8.859334150163942E-5 26-27 2.2148335375409854E-4 0.0 0.0 7.75191738139345E-4 1.3289001225245912E-4 28-29 3.1007669525573797E-4 0.0 0.0 0.0023698718851688544 1.3289001225245912E-4 30-31 3.1007669525573797E-4 0.0 0.0 0.006312275581991809 1.3289001225245912E-4 32-33 3.1007669525573797E-4 0.0 0.0 0.014750791360022964 1.3289001225245912E-4 34-35 3.322250306311478E-4 0.0 0.0 0.027242452511754126 1.7718668300327885E-4 36-37 3.543733660065577E-4 0.0 0.0 0.04965656791166889 1.7718668300327885E-4 38-39 3.9867003675737736E-4 0.0 0.0 0.08121794582162795 1.7718668300327885E-4 40-41 3.9867003675737736E-4 0.0 0.0 0.11654454074540666 1.7718668300327885E-4 42-43 3.9867003675737736E-4 0.0 0.0 0.15479471593873947 1.7718668300327885E-4 44-45 4.429667075081971E-4 0.0 0.0 0.20529292059467394 1.7718668300327885E-4 46-47 4.429667075081971E-4 0.0 0.0 0.2688143464513494 1.7718668300327885E-4 48-49 4.429667075081971E-4 0.0 0.0 0.33539224258983147 1.7718668300327885E-4 50-51 4.429667075081971E-4 0.0 0.0 0.40912405105457084 1.7718668300327885E-4 52-53 4.429667075081971E-4 0.0 0.0 0.484406242995589 2.6578002450491824E-4 54-55 4.429667075081971E-4 0.0 0.0 0.5737083312292415 2.6578002450491824E-4 56-57 4.872633782590168E-4 0.0 0.0 0.6805740494155941 3.1007669525573797E-4 58-59 4.872633782590168E-4 0.0 0.0 0.7990676436740367 3.543733660065577E-4 60-61 4.872633782590168E-4 0.0 0.0 0.9296099323767024 3.543733660065577E-4 62-63 4.872633782590168E-4 0.0 0.0 1.0667302766858648 3.543733660065577E-4 64-65 4.872633782590168E-4 0.0 0.0 1.2121340984254307 3.543733660065577E-4 66-67 4.872633782590168E-4 0.0 0.0 1.38179034740107 3.9867003675737736E-4 68-69 4.872633782590168E-4 0.0 0.0 1.5805273607246226 3.9867003675737736E-4 70-71 4.872633782590168E-4 0.0 0.0 1.8049342947482754 3.9867003675737736E-4 72-73 4.872633782590168E-4 0.0 0.0 2.0320211773523527 5.758567197606563E-4 74-75 4.872633782590168E-4 0.0 0.0 2.2694291842413707 7.530434027639351E-4 76-77 4.872633782590168E-4 0.0 0.0 2.5364273671919366 7.530434027639351E-4 78-79 4.872633782590168E-4 0.0 0.0 2.8445550089346385 7.530434027639351E-4 80-81 5.758567197606563E-4 0.0 0.0 3.1814976350007487 7.530434027639351E-4 82-83 5.758567197606563E-4 0.0 0.0 3.5288499786933016 7.530434027639351E-4 84-85 5.98005055136066E-4 0.0 0.0 3.8877194567810673 7.530434027639351E-4 86-87 6.201533905114759E-4 0.0 0.0 4.25750806420891 7.530434027639351E-4 88-89 6.201533905114759E-4 0.0 0.0 4.672833649168596 7.530434027639351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8075 0.0 24.573738 1 TACATGG 8110 0.0 23.889893 2 ACATGGG 8145 0.0 22.740364 3 GTACCTG 1895 0.0 22.04504 1 GTATCAA 13645 0.0 21.848598 1 GAGTACT 6850 0.0 20.842028 12-13 TACCTGG 2055 0.0 20.285355 2 AGTACTT 7220 0.0 20.06908 12-13 CTAGTAC 455 0.0 19.781261 3 GTACTTT 7215 0.0 19.691387 14-15 AGAGTAC 11465 0.0 19.41207 10-11 GGTATCA 10660 0.0 18.74832 1 ATCAACG 15760 0.0 18.725939 3 TCAACGC 15815 0.0 18.630861 4 GTGTAGC 1200 0.0 18.593197 1 TATCAAC 15950 0.0 18.59197 2 CAACGCA 15870 0.0 18.566294 5 AACGCAG 16160 0.0 18.256351 6 GTATAGC 765 0.0 17.99592 1 CATGGGG 4720 0.0 17.964815 4 >>END_MODULE