FastQCFastQC Report
Fri 27 May 2016
SRR939151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257506
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66040.29253521363841334No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63310.2804422225234396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55760.2469982361065707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.15703169781165588No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29450.13045369536116405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG76800.022.2569891
GAGTACT72350.022.07015612-13
TACATGG77650.021.6928652
GTATCAA148100.020.9938131
ACATGGG79050.020.3482863
GTACTTT78850.020.16053214-15
AGTACTT76200.020.05209712-13
CGCGGAA5150.019.91413582-83
CTCGCGC5250.019.43291960-61
TGTACCG2953.0613592E-919.301425
AGAGTAC119000.018.86136210-11
ATTCGCG5100.018.62593780-81
GGTATCA118150.018.618361
ATCAACG167700.018.44783
TCAACGC168100.018.4039024
CAACGCA168450.018.3656625
TATCAAC170400.018.1833382
TCGCGCT5900.018.09628360-61
AACGCAG171900.017.9694656
GTGTAGC10900.017.9097921
Produced by FastQC (version 0.11.5)