##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939151_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2257506 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4404298371743 34.0 31.0 34.0 31.0 34.0 2 32.656392053886016 34.0 31.0 34.0 31.0 34.0 3 32.748700557163524 34.0 31.0 34.0 31.0 34.0 4 36.22522775133267 37.0 35.0 37.0 35.0 37.0 5 36.18059353995073 37.0 35.0 37.0 35.0 37.0 6 36.21595424331098 37.0 37.0 37.0 35.0 37.0 7 36.168554590774065 37.0 37.0 37.0 35.0 37.0 8 36.15279870795471 37.0 36.0 37.0 35.0 37.0 9 37.885715918363005 39.0 38.0 39.0 35.0 39.0 10-11 37.8204312635271 39.0 38.0 39.0 35.0 39.0 12-13 37.747283728149554 39.0 38.0 39.0 35.0 39.0 14-15 39.095772502930224 40.0 38.0 41.0 36.0 41.0 16-17 39.03355561402716 40.0 38.0 41.0 36.0 41.0 18-19 39.049694220081804 40.0 38.5 41.0 35.5 41.0 20-21 39.12280343883914 40.0 39.0 41.0 36.0 41.0 22-23 39.085828786280075 40.0 39.0 41.0 35.5 41.0 24-25 39.02339750149058 40.0 38.5 41.0 35.0 41.0 26-27 38.88775910230139 40.0 38.0 41.0 35.0 41.0 28-29 38.73428996423487 40.0 38.0 41.0 35.0 41.0 30-31 38.54436289427359 40.0 38.0 41.0 34.0 41.0 32-33 38.41369480302599 40.0 38.0 41.0 34.0 41.0 34-35 38.37031662374319 40.0 38.0 41.0 34.0 41.0 36-37 38.260795320145334 40.0 38.0 41.0 34.0 41.0 38-39 38.1282552515918 40.0 38.0 41.0 33.5 41.0 40-41 37.96266410809096 40.0 38.0 41.0 33.0 41.0 42-43 37.799478938261956 40.0 37.0 41.0 33.0 41.0 44-45 37.578800455015404 40.0 37.0 41.0 33.0 41.0 46-47 37.2956909084627 40.0 37.0 41.0 32.0 41.0 48-49 36.98661421054916 40.0 36.5 41.0 31.0 41.0 50-51 36.719617799465425 40.0 36.0 41.0 31.0 41.0 52-53 36.48159783406999 39.0 36.0 41.0 30.0 41.0 54-55 36.22949617852622 39.0 35.0 41.0 30.0 41.0 56-57 35.9420610620747 39.0 35.0 40.0 29.5 41.0 58-59 35.63099765847798 38.0 35.0 40.0 28.5 41.0 60-61 35.25618669452041 38.0 34.5 40.0 28.0 41.0 62-63 34.86259549254797 37.5 34.0 40.0 27.5 41.0 64-65 34.43887967518137 37.0 34.0 39.5 26.5 41.0 66-67 34.06456084723584 36.0 33.5 39.0 26.0 40.5 68-69 33.58527972904612 36.0 33.0 39.0 26.0 40.0 70-71 33.124218053019575 35.0 33.0 38.5 25.0 40.0 72-73 32.63540495573434 35.0 32.0 37.5 23.5 39.5 74-75 32.12488626829785 35.0 32.0 37.0 22.5 39.0 76-77 30.14592386465418 33.5 29.5 35.0 20.0 37.5 78-79 31.141978360190407 34.0 31.0 36.0 20.0 38.0 80-81 31.17032224942038 35.0 31.0 36.0 20.0 37.0 82-83 30.87201385068301 34.0 31.0 35.0 19.5 37.0 84-85 30.483681106495396 34.0 31.0 35.0 18.0 36.5 86-87 29.909125601438046 34.0 30.0 35.0 10.0 36.0 88-89 29.352133726333395 34.0 29.5 35.0 4.5 36.0 90-91 28.8807183679689 34.0 29.0 35.0 2.0 35.0 92-93 28.575614859938355 34.0 29.0 35.0 2.0 35.0 94-95 28.20930509154793 34.0 29.0 35.0 2.0 35.0 96-97 27.596265746359038 33.0 27.5 35.0 2.0 35.0 98-99 26.770227631731654 33.0 26.0 35.0 2.0 35.0 100-101 23.110705353607035 28.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.062388229425373254 1101 2 0.13664365974281623 1101 3 0.18927990661686067 1101 4 0.11308727643863392 1101 5 0.10130566546574471 1101 6 0.1585257940654472 1101 7 0.09133096998324675 1101 8 0.13847753901556104 1101 9 0.141637679749536 1101 10-11 0.1354392793518855 1101 12-13 0.1184991274239593 1101 14-15 0.2235884452680139 1101 16-17 0.22491459349827636 1101 18-19 0.2561276288569658 1101 20-21 0.23032498856721162 1101 22-23 0.24222882310699845 1101 24-25 0.20422775038715457 1101 26-27 0.11986327540206076 1101 28-29 0.12036802749682352 1101 30-31 0.20170364180254552 1101 32-33 0.1892125415162269 1101 34-35 0.26056457531775123 1101 36-37 0.24187538190607683 1101 38-39 0.3538739605141572 1101 40-41 0.341141027422978 1101 42-43 0.3834996992219857 1101 44-45 0.2784555383744376 1101 46-47 0.21185977145930934 1101 48-49 0.09957712009762076 1101 50-51 0.09396197041926513 1101 52-53 0.10594612996184338 1101 54-55 0.04739800364728808 1101 56-57 0.048496909802921095 1101 58-59 0.07435979538557547 1101 60-61 0.16006801928783432 1101 62-63 0.08964979865607603 1101 64-65 0.0530536741215073 1101 66-67 0.0850881913240471 1101 68-69 0.056674890341163575 1101 70-71 -0.007705602784021437 1101 72-73 -0.08693702516099222 1101 74-75 -0.06798063712149371 1101 76-77 -0.08548039478321812 1101 78-79 0.06606869038212793 1101 80-81 0.0275758951849312 1101 82-83 0.08528023877217095 1101 84-85 -0.05303257612609258 1101 86-87 -0.06592299455008899 1101 88-89 0.1760936925241232 1101 90-91 0.1240116482255118 1101 92-93 0.04189028576085718 1101 94-95 -0.12276930501183614 1101 96-97 -0.0662255317103515 1101 98-99 -0.27706903586163634 1101 100-101 -0.8741086443035648 1102 1 0.1588626239783082 1102 2 0.12414974855404637 1102 3 0.1622919636035718 1102 4 0.0661298066267193 1102 5 0.032612976955896045 1102 6 0.09088993564317605 1102 7 0.12362891395452635 1102 8 0.13380011549128312 1102 9 0.16921739692457294 1102 10-11 0.17666541007137937 1102 12-13 0.20891955825582897 1102 14-15 0.23115390410713843 1102 16-17 0.2861319198347374 1102 18-19 0.29839981861398 1102 20-21 0.24054696850416946 1102 22-23 0.17265759706751993 1102 24-25 0.2509028919694387 1102 26-27 0.17660161897169502 1102 28-29 0.15694587988629394 1102 30-31 0.2675769762814113 1102 32-33 0.19709378219926776 1102 34-35 0.2141042211453552 1102 36-37 0.2666577940231818 1102 38-39 0.34591719576251023 1102 40-41 0.31861914191645013 1102 42-43 0.34639583943520336 1102 44-45 0.3143114769738773 1102 46-47 0.2036845444689348 1102 48-49 0.1402292172083932 1102 50-51 0.17057720975706303 1102 52-53 0.1577206667940132 1102 54-55 0.11372156801837718 1102 56-57 -0.018991575310984388 1102 58-59 0.06090155842145606 1102 60-61 0.06359882018256258 1102 62-63 0.008278190333022906 1102 64-65 -0.11678487670847204 1102 66-67 -0.13833696711175492 1102 68-69 -0.05211993813556859 1102 70-71 -0.047678490150261155 1102 72-73 -0.17055800888985573 1102 74-75 -0.09265179062745688 1102 76-77 -0.14489464734077373 1102 78-79 -0.09759298003793404 1102 80-81 -0.19507691560295015 1102 82-83 -0.1498862512492778 1102 84-85 -0.03194944947020062 1102 86-87 0.04634796857439483 1102 88-89 0.21013489926695783 1102 90-91 0.2614204681188568 1102 92-93 0.15554108375627607 1102 94-95 -0.0361739908494485 1102 96-97 0.24078183467413794 1102 98-99 0.1589611909994879 1102 100-101 0.04541691248369162 1103 1 0.09486354001445818 1103 2 0.057746069705196135 1103 3 0.08399099207868232 1103 4 0.10017788660204019 1103 5 0.16183567259593445 1103 6 0.17479641252543132 1103 7 0.14372110375284564 1103 8 0.11749775202860491 1103 9 0.1468184751050785 1103 10-11 0.16196499214230187 1103 12-13 0.20715939281294027 1103 14-15 0.22409734582980434 1103 16-17 0.21950432379872353 1103 18-19 0.33098976675659486 1103 20-21 0.2128240833479751 1103 22-23 0.1483740699706786 1103 24-25 0.12044349785482211 1103 26-27 0.18860770335928834 1103 28-29 0.26457528365307326 1103 30-31 0.26699327645910387 1103 32-33 0.31057049207959153 1103 34-35 0.23584194767676792 1103 36-37 0.35367452161817425 1103 38-39 0.3775739693381439 1103 40-41 0.3753547646446833 1103 42-43 0.4294873720123604 1103 44-45 0.3985223471038921 1103 46-47 0.3559907052094289 1103 48-49 0.30705894725095106 1103 50-51 0.32806662491965 1103 52-53 0.35347135692591536 1103 54-55 0.28191342990157864 1103 56-57 0.28084285264580444 1103 58-59 0.20608745066997614 1103 60-61 0.25172539710351316 1103 62-63 0.16751361311300883 1103 64-65 0.047902799177670374 1103 66-67 0.1734624378621561 1103 68-69 0.126130561983409 1103 70-71 -0.16875323303670342 1103 72-73 -0.15833372012188818 1103 74-75 -0.03926921289974672 1103 76-77 -0.05563427698199419 1103 78-79 -0.03099692863061776 1103 80-81 -0.17737106454625362 1103 82-83 -0.07323404543522827 1103 84-85 -0.015504265838238496 1103 86-87 0.2273023649766195 1103 88-89 0.11571176607559508 1103 90-91 0.11769134159707306 1103 92-93 0.10907149898790891 1103 94-95 -0.004163271636883792 1103 96-97 -0.04567071599473138 1103 98-99 -0.258794510505858 1103 100-101 -0.32735850814033185 1104 1 0.15297260282117975 1104 2 0.13663180201772462 1104 3 0.12525016744234563 1104 4 0.08088293028594506 1104 5 0.17031194363710966 1104 6 0.13389507573928938 1104 7 0.12078457095202566 1104 8 0.1015184921395047 1104 9 0.06794315352247082 1104 10-11 0.18391650041495922 1104 12-13 0.16186097529231347 1104 14-15 0.171699657883444 1104 16-17 0.22350821789594022 1104 18-19 0.16555733650969273 1104 20-21 0.19311112411978115 1104 22-23 0.24626773719629824 1104 24-25 0.18362767044632733 1104 26-27 0.14290629374645647 1104 28-29 0.23269218113934187 1104 30-31 0.38821169986784554 1104 32-33 0.3024932183156466 1104 34-35 0.3253204766357882 1104 36-37 0.3441765463840838 1104 38-39 0.4422351339743926 1104 40-41 0.5079312944045995 1104 42-43 0.42947308752245306 1104 44-45 0.3809922516205475 1104 46-47 0.4240049722674186 1104 48-49 0.31077666733015263 1104 50-51 0.3500158331473173 1104 52-53 0.40582849144755073 1104 54-55 0.3199238522527921 1104 56-57 0.5320871769821665 1104 58-59 0.5025573174174696 1104 60-61 0.5051435060597953 1104 62-63 0.4589020752157822 1104 64-65 0.4820976647993618 1104 66-67 0.4709784619123951 1104 68-69 0.21929037346688318 1104 70-71 0.17405467156421395 1104 72-73 0.271336109631207 1104 74-75 0.14268407730249777 1104 76-77 0.19001173786322312 1104 78-79 0.24394790660626242 1104 80-81 0.3814491963805331 1104 82-83 0.4857403185054885 1104 84-85 0.4523001886951974 1104 86-87 0.40102742583096784 1104 88-89 0.3577628106107653 1104 90-91 0.47301475939612914 1104 92-93 0.508059454983929 1104 94-95 0.3246311852062824 1104 96-97 0.42992144874465765 1104 98-99 0.31458660888487344 1104 100-101 0.11775769739556452 1105 1 0.05243189957456451 1105 2 0.14884586916613074 1105 3 0.10571384632961411 1105 4 0.07366616700797124 1105 5 0.029566131409609397 1105 6 0.029725446715637815 1105 7 0.03067336484030392 1105 8 0.07113209614454519 1105 9 0.12474354021393452 1105 10-11 0.10415434613356211 1105 12-13 0.09080953554065019 1105 14-15 0.046780911103567746 1105 16-17 0.11266751483398707 1105 18-19 0.0964429319757727 1105 20-21 0.18107664505448895 1105 22-23 0.15133500655257137 1105 24-25 0.08790375965497788 1105 26-27 0.058175805137601344 1105 28-29 0.19009956631838065 1105 30-31 0.2167582875095988 1105 32-33 0.1747434200636988 1105 34-35 0.1894985633809867 1105 36-37 0.16832657306838428 1105 38-39 0.2171917604514917 1105 40-41 0.21418428369209863 1105 42-43 0.17951532996359987 1105 44-45 0.28844668056738243 1105 46-47 0.2212523347694244 1105 48-49 0.13829835639992893 1105 50-51 0.11919632517250278 1105 52-53 0.03150356259028797 1105 54-55 0.21704434893156588 1105 56-57 0.14052236922913863 1105 58-59 0.3248933428792782 1105 60-61 0.36086802625030145 1105 62-63 0.3199003218082197 1105 64-65 0.0981593489200776 1105 66-67 0.14205665521011213 1105 68-69 -0.024952074138646196 1105 70-71 0.007200606964204326 1105 72-73 -0.07484819760199457 1105 74-75 -0.022922104036233293 1105 76-77 0.10916362967825322 1105 78-79 0.25107960791942574 1105 80-81 0.09306951485622506 1105 82-83 0.0955322317787548 1105 84-85 0.2637080023003122 1105 86-87 0.25976604807182113 1105 88-89 0.48201802789849424 1105 90-91 0.44041328982084593 1105 92-93 0.35095208347020446 1105 94-95 0.15051338281042348 1105 96-97 0.018115586694158026 1105 98-99 0.16691742213727423 1105 100-101 -0.27640490507121385 1106 1 -0.06438539229309725 1106 2 -0.0044921224348044575 1106 3 -0.015026321651767205 1106 4 -0.0659417581949171 1106 5 -0.009476417253921454 1106 6 -0.050129555838132944 1106 7 0.012259611364342504 1106 8 -0.07597221121599063 1106 9 -0.08977677645368232 1106 10-11 -0.030891373462367255 1106 12-13 0.03038979489029714 1106 14-15 -0.02057302737892286 1106 16-17 -0.0794328199937766 1106 18-19 -0.1486313047304364 1106 20-21 -0.022345408177450565 1106 22-23 -0.06934450936594061 1106 24-25 0.049695620219068815 1106 26-27 0.0042433523823675046 1106 28-29 0.03711005806119516 1106 30-31 0.10568964072451337 1106 32-33 0.18594809062722817 1106 34-35 -0.049657663564140364 1106 36-37 0.033630223542253646 1106 38-39 0.04368873139524965 1106 40-41 -0.024523259863883595 1106 42-43 -0.10667373422117521 1106 44-45 -0.06675183966416398 1106 46-47 0.06511938261007089 1106 48-49 0.1393056772103236 1106 50-51 0.14932905032998178 1106 52-53 0.10217208310557879 1106 54-55 0.1145033467182941 1106 56-57 0.036000420985565995 1106 58-59 0.012264767599781123 1106 60-61 -0.0855719459419717 1106 62-63 -0.032485503343586686 1106 64-65 0.06358057927022998 1106 66-67 0.12433277461309444 1106 68-69 -0.07085078874053607 1106 70-71 0.12256544153659377 1106 72-73 0.09926312553517391 1106 74-75 0.11060997416591789 1106 76-77 0.202720323395976 1106 78-79 0.3303174491316163 1106 80-81 0.2645978049646196 1106 82-83 0.18676836338309144 1106 84-85 0.1595159161531008 1106 86-87 0.1866235618338088 1106 88-89 0.14159679754834542 1106 90-91 0.4029437931974975 1106 92-93 0.28467551715728234 1106 94-95 0.19855088068227644 1106 96-97 0.3531712376220284 1106 98-99 0.26528460476028215 1106 100-101 -0.21792886795640598 1107 1 -0.08048524201473839 1107 2 -0.09981876439661619 1107 3 -0.046050690653444804 1107 4 -0.0462179373677003 1107 5 0.05032945897136187 1107 6 -0.04838598536100136 1107 7 0.05759778035918117 1107 8 0.03588355571594093 1107 9 0.17971543639512078 1107 10-11 0.15311796343437578 1107 12-13 0.05262608199026175 1107 14-15 0.11635809219482951 1107 16-17 0.12209643305448736 1107 18-19 0.06415377587340743 1107 20-21 0.1206179785502357 1107 22-23 0.14170585949911896 1107 24-25 0.10141773760696537 1107 26-27 0.1498334083952173 1107 28-29 0.09698434936213118 1107 30-31 0.09797538733472066 1107 32-33 0.14793962039259156 1107 34-35 0.19472365495767008 1107 36-37 0.16075898903970653 1107 38-39 0.20078180578303773 1107 40-41 0.23167390790140985 1107 42-43 0.19475860636422482 1107 44-45 0.129868925218517 1107 46-47 0.13175950863895736 1107 48-49 0.05255963055572721 1107 50-51 0.001272636176494757 1107 52-53 0.12462802422378871 1107 54-55 0.028169284762626035 1107 56-57 0.15067454749262055 1107 58-59 0.03402001453444825 1107 60-61 0.1430127478658818 1107 62-63 0.10009789687499904 1107 64-65 0.1371286000359575 1107 66-67 0.20395596871125576 1107 68-69 0.19934478658648658 1107 70-71 0.13374773089073244 1107 72-73 0.11941803605124335 1107 74-75 0.13196593748433116 1107 76-77 0.057154815694211436 1107 78-79 0.22766153533674682 1107 80-81 0.34488060150821553 1107 82-83 0.229495979592663 1107 84-85 0.32615177291050657 1107 86-87 0.34084480402104234 1107 88-89 0.5355258934874136 1107 90-91 0.6026432364844965 1107 92-93 0.4824526142234369 1107 94-95 0.379156220687058 1107 96-97 0.501211748739216 1107 98-99 0.6526421178242501 1107 100-101 0.15259031814967372 1108 1 0.08261718426953024 1108 2 0.05130697910579585 1108 3 0.05291383000497518 1108 4 0.0018173331996322872 1108 5 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76-77 -0.10664171312628312 2316 78-79 0.10334967339710843 2316 80-81 0.11703600793258673 2316 82-83 -0.04762801967297747 2316 84-85 -0.25898924785493804 2316 86-87 -0.3349906985262834 2316 88-89 -0.3570026122793095 2316 90-91 -0.36906204437680756 2316 92-93 -0.2625750227648851 2316 94-95 0.08055249510301365 2316 96-97 -0.10101756941727302 2316 98-99 -0.08832365230681916 2316 100-101 0.20665710262050752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 18.0 5 44.0 6 76.0 7 200.0 8 518.0 9 1000.0 10 1669.0 11 2551.0 12 3807.0 13 5679.0 14 7011.0 15 9305.0 16 12095.0 17 16990.0 18 16827.0 19 12058.0 20 11286.0 21 12124.0 22 12649.0 23 13636.0 24 16057.0 25 18731.0 26 22146.0 27 27168.0 28 32802.0 29 39778.0 30 48593.0 31 60353.0 32 76128.0 33 98815.0 34 136289.0 35 199106.0 36 320484.0 37 530721.0 38 453053.0 39 37726.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77215797473755 19.146347967537487 14.746886638992345 23.33460741873261 2 15.774753201098912 20.350289212963332 39.72020450886952 24.154753077068232 3 19.850401283540332 23.767378691352313 29.63744060923869 26.744779415868663 4 13.552832196237796 16.564075577207767 36.02271710462785 33.86037512192658 5 14.281069463381272 36.81540602771377 33.8070419303426 15.096482578562359 6 32.93058800286688 35.26794613170463 16.933686997952606 14.867778867475877 7 29.196334361902025 32.22068955741425 20.23175132203414 18.35122475864959 8 26.175700086732885 35.32070346656886 19.245796024462393 19.257800422235867 9 26.045202094700965 17.549233534705998 20.386922559674257 36.01864181091877 10-11 25.504051816473577 26.266463965101313 26.671623464123684 21.557860754301426 12-13 26.304071838568753 23.458874527908232 27.465508397319876 22.771545236203135 14-15 22.768492233491173 25.042685842608236 25.984797660578835 26.204024263321756 16-17 22.626867443541194 28.379095219437563 26.45310701452891 22.540930322492336 18-19 22.362576223496195 27.055542709521035 28.56645785216075 22.015423214822018 20-21 23.161953943865488 26.009698313094187 28.550666089038078 22.277681654002247 22-23 22.7924754131329 25.970030644436825 28.545040411852725 22.69245353057755 24-25 22.934210529230057 26.189431522928164 28.51997777183708 22.356380176004702 26-27 22.61086159460318 26.269614264065595 28.728709085408177 22.39081505592305 28-29 22.222975265624985 26.80823439671921 28.696579322491278 22.272211015164523 30-31 22.535088980236235 26.209008037646193 28.497884439427946 22.758018542689626 32-33 22.35670691462171 26.44810246351505 28.577044756470194 22.61814586539305 34-35 23.094980937183525 26.33592554559172 28.221540672510294 22.347552844714468 36-37 22.970907491739048 26.113469976438424 28.387411228691285 22.52821130313124 38-39 22.544045756323825 26.50182244169748 28.550936763610267 22.403195038368416 40-41 22.725546921379774 26.430634685758335 28.37810896782711 22.465709425034778 42-43 22.707076327529922 26.463058953410663 27.942725390522295 22.887139328537117 44-45 22.758626646971777 26.4332118017833 27.935135331483007 22.87302621976191 46-47 22.96374210098382 26.78029777001396 27.643738229411063 22.61222189959116 48-49 23.107694140905576 26.74830746796055 27.477657159576086 22.666341231557784 50-51 22.664435886327656 26.936229626853702 27.667168990912984 22.732165495905658 52-53 23.112765938125897 27.00908326387977 27.20385594086565 22.674294857128682 54-55 22.724900841902524 26.55935355210573 27.46298348708708 23.25276211890467 56-57 22.88487826831911 27.023826293263454 27.313858745004442 22.777436693412998 58-59 23.263868585330762 26.620179639975834 27.27418308818448 22.841768686508928 60-61 22.97257007219754 26.63642819518544 27.841751064002278 22.549250668614736 62-63 22.71890433872756 26.752725354199697 27.744679418431627 22.78369088864111 64-65 22.544023977358655 26.86447131137919 27.51418295670848 23.077321754553672 66-67 22.636447702845626 26.916082018682896 27.25483269008212 23.19263758838936 68-69 22.4063452757619 26.82967670058064 27.552822848131335 23.211155175526123 70-71 22.68514960428668 26.827066423536955 27.348969913916044 23.138814058260323 72-73 22.700781304678703 26.572996926696984 27.545663223043483 23.18055854558083 74-75 22.44206659915854 27.112242448082085 27.190558961969536 23.255131990789838 76-77 22.605613920448743 26.772284286402986 27.228206683044153 23.393895110104115 78-79 22.610902017918498 26.808067953060455 27.323372607612978 23.25765742140807 80-81 22.572637911253064 27.03926589085819 27.28669327509602 23.101402922792733 82-83 22.627344051253235 26.822743689746588 27.366487611387214 23.183424647612963 84-85 22.468157338230775 26.818644114345652 27.226262078594697 23.486936468828876 86-87 22.505617225181666 26.72029876942083 27.33510057349574 23.438983431901757 88-89 22.435016230654522 26.737662901380148 27.094497143235802 23.732823724729524 90-91 22.427631232278415 26.642503167735104 27.246479472920893 23.683386127065585 92-93 22.586363004129336 26.543163118946307 27.32747997126032 23.54299390566404 94-95 22.348534178868185 26.403982979447232 27.3346117352512 23.912871106433382 96-97 22.176640948019628 26.3576265135065 27.511687676577605 23.95404486189627 98-99 22.18548495518105 26.459204468362056 27.09212497256333 24.263185603893557 100-101 22.304902452610584 25.70467011518311 27.73557387233845 24.25485355986786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 392.0 1 267.0 2 196.0 3 275.5 4 262.0 5 271.0 6 470.0 7 1010.5 8 1661.0 9 2015.5 10 1928.0 11 1764.0 12 1879.5 13 2025.5 14 2242.5 15 2471.5 16 2602.5 17 2749.0 18 2910.0 19 3045.5 20 3336.0 21 3898.5 22 4639.5 23 5639.0 24 7200.0 25 9136.0 26 11239.5 27 13588.5 28 15989.5 29 18900.5 30 22482.5 31 26377.0 32 30582.5 33 34106.0 34 37709.0 35 41390.0 36 45830.5 37 50352.0 38 53537.0 39 56499.0 40 58574.0 41 63428.0 42 69257.5 43 74222.0 44 79531.0 45 83579.0 46 85445.0 47 90817.5 48 104265.0 49 105169.5 50 94541.0 51 89260.5 52 85224.0 53 83398.0 54 81479.5 55 74496.0 56 66338.0 57 59086.5 58 52574.5 59 46108.0 60 38707.0 61 31469.0 62 26069.5 63 21131.0 64 16639.5 65 13385.5 66 10249.0 67 8449.5 68 6288.0 69 3894.5 70 2846.0 71 2149.5 72 1690.5 73 1442.5 74 1193.5 75 949.0 76 639.0 77 346.0 78 201.5 79 129.5 80 70.0 81 40.5 82 24.0 83 11.0 84 7.5 85 5.5 86 4.0 87 3.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33337674407066914 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010852684333950828 16-17 0.001838311836159018 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.2081837213278725E-4 26-27 0.0012846034517737717 28-29 0.0 30-31 0.002945728604929511 32-33 0.0 34-35 0.010276827614190173 36-37 0.02894787433566068 38-39 0.02243626373529018 40-41 0.014440714664767226 42-43 0.010653349315572139 44-45 0.0029900252756803304 46-47 0.0011295651041459025 48-49 6.865983966377055E-4 50-51 0.0 52-53 1.1074167687704927E-4 54-55 0.0 56-57 0.0 58-59 0.00451826041658361 60-61 0.010055344260436075 62-63 0.004119590379826233 64-65 0.0019047568422852475 66-67 0.0041417387152016425 68-69 0.003477288653939347 70-71 2.2148335375409852E-5 72-73 0.0 74-75 0.0 76-77 0.213509953018951 78-79 2.8792835988032813E-4 80-81 0.003366546977062298 82-83 4.2081837213278725E-4 84-85 0.0 86-87 0.10582474642370829 88-89 0.3979169933546135 90-91 0.38358702036672326 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015282351409032801 100-101 5.980050551360662E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2257506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.80185888440588 #Duplication Level Percentage of deduplicated Percentage of total 1 75.31934823469577 40.52320944988527 2 13.92008726844768 14.978531417512745 3 4.597419167507347 7.420490918480792 4 2.038681974504174 4.38739519609822 5 1.1111494334782934 2.9890952509743345 6 0.6825485362175441 2.203342801640046 7 0.44336055414858755 1.669753537845001 8 0.30890222992134536 1.3295611346645217 9 0.23331223979820753 1.1297368981465044 >10 1.194536645825863 11.577530627212553 >50 0.09280133608081881 3.4119898343548267 >100 0.052671824506579666 5.164973317315312 >500 0.00353593427477966 1.170638624126722 >1k 0.0013979275039824406 1.224790614902493 >5k 2.4669308893807775E-4 0.8189603768407762 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6604 0.29253521363841334 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6331 0.2804422225234396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5576 0.2469982361065707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3545 0.15703169781165588 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2945 0.13045369536116405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.429667075081971E-5 2 0.0 0.0 0.0 0.0 4.429667075081971E-5 3 0.0 0.0 0.0 0.0 4.429667075081971E-5 4 0.0 0.0 0.0 0.0 4.429667075081971E-5 5 0.0 0.0 0.0 0.0 4.429667075081971E-5 6 0.0 0.0 0.0 0.0 4.429667075081971E-5 7 0.0 0.0 0.0 0.0 4.429667075081971E-5 8 0.0 0.0 0.0 0.0 4.429667075081971E-5 9 0.0 0.0 0.0 4.429667075081971E-5 4.429667075081971E-5 10-11 0.0 0.0 0.0 4.429667075081971E-5 4.429667075081971E-5 12-13 4.429667075081971E-5 0.0 0.0 4.429667075081971E-5 1.5503834762786898E-4 14-15 8.859334150163942E-5 0.0 0.0 4.429667075081971E-5 1.7718668300327885E-4 16-17 8.859334150163942E-5 0.0 0.0 4.429667075081971E-5 2.2148335375409854E-4 18-19 8.859334150163942E-5 0.0 0.0 6.644500612622956E-5 2.6578002450491824E-4 20-21 8.859334150163942E-5 0.0 0.0 1.3289001225245912E-4 2.6578002450491824E-4 22-23 8.859334150163942E-5 0.0 0.0 1.7718668300327885E-4 2.6578002450491824E-4 24-25 8.859334150163942E-5 0.0 0.0 4.2081837213278725E-4 2.6578002450491824E-4 26-27 8.859334150163942E-5 0.0 0.0 7.308950673885252E-4 2.6578002450491824E-4 28-29 1.1074167687704927E-4 0.0 0.0 0.002325575214418035 2.6578002450491824E-4 30-31 1.3289001225245912E-4 0.0 0.0 0.006245830575865579 2.6578002450491824E-4 32-33 1.3289001225245912E-4 0.0 0.0 0.015038719719903292 2.6578002450491824E-4 34-35 1.3289001225245912E-4 0.0 0.0 0.027796160896139368 2.6578002450491824E-4 36-37 1.3289001225245912E-4 0.0 0.0 0.05045390798518365 2.6578002450491824E-4 38-39 1.7718668300327885E-4 0.0 0.0 0.08250254927340171 2.8792835988032813E-4 40-41 1.7718668300327885E-4 0.0 0.0 0.11774055085567879 3.1007669525573797E-4 42-43 1.7718668300327885E-4 0.0 0.0 0.15661087943952307 3.1007669525573797E-4 44-45 2.2148335375409854E-4 0.0 0.0 0.2075741991383412 3.543733660065577E-4 46-47 2.6578002450491824E-4 0.0 0.0 0.2717157783855281 3.9867003675737736E-4 48-49 2.6578002450491824E-4 0.0 0.0 0.33882523457301994 4.2081837213278725E-4 50-51 2.6578002450491824E-4 0.0 0.0 0.4131107514221446 4.429667075081971E-4 52-53 2.8792835988032813E-4 0.0 0.0 0.489057393424425 5.315600490098365E-4 54-55 3.1007669525573797E-4 0.0 0.0 0.5790017833839645 5.315600490098365E-4 56-57 3.543733660065577E-4 0.0 0.0 0.6861332815948219 5.315600490098365E-4 58-59 3.543733660065577E-4 0.0 0.0 0.8051584359022745 5.315600490098365E-4 60-61 3.543733660065577E-4 0.0 0.0 0.9365202130138304 5.315600490098365E-4 62-63 3.543733660065577E-4 0.0 0.0 1.0738620406767467 5.315600490098365E-4 64-65 3.543733660065577E-4 0.0 0.0 1.220107499160578 5.758567197606563E-4 66-67 3.543733660065577E-4 0.0 0.0 1.3899852314899717 5.98005055136066E-4 68-69 3.543733660065577E-4 0.0 0.0 1.5901397382775504 6.201533905114759E-4 70-71 3.543733660065577E-4 0.0 0.0 1.8155211990577214 6.644500612622956E-4 72-73 3.543733660065577E-4 0.0 0.0 2.0435826084183164 6.865983966377055E-4 74-75 3.543733660065577E-4 0.0 0.0 2.28289537214962 7.087467320131154E-4 76-77 3.543733660065577E-4 0.0 0.0 2.551089565210458 7.087467320131154E-4 78-79 3.543733660065577E-4 0.0 0.0 2.8603910687280565 7.087467320131154E-4 80-81 3.543733660065577E-4 0.0 0.0 3.19804244152618 7.087467320131154E-4 82-83 3.543733660065577E-4 0.0 0.0 3.5455941202371113 7.530434027639351E-4 84-85 3.7652170138196753E-4 0.0 0.0 3.9041535216296213 7.530434027639351E-4 86-87 3.9867003675737736E-4 0.0 0.0 4.274030722398966 7.530434027639351E-4 88-89 3.9867003675737736E-4 0.0 0.0 4.688426077272885 7.530434027639351E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7680 0.0 22.256989 1 GAGTACT 7235 0.0 22.070156 12-13 TACATGG 7765 0.0 21.692865 2 GTATCAA 14810 0.0 20.993813 1 ACATGGG 7905 0.0 20.348286 3 GTACTTT 7885 0.0 20.160532 14-15 AGTACTT 7620 0.0 20.052097 12-13 CGCGGAA 515 0.0 19.914135 82-83 CTCGCGC 525 0.0 19.432919 60-61 TGTACCG 295 3.0613592E-9 19.30142 5 AGAGTAC 11900 0.0 18.861362 10-11 ATTCGCG 510 0.0 18.625937 80-81 GGTATCA 11815 0.0 18.61836 1 ATCAACG 16770 0.0 18.4478 3 TCAACGC 16810 0.0 18.403902 4 CAACGCA 16845 0.0 18.365662 5 TATCAAC 17040 0.0 18.183338 2 TCGCGCT 590 0.0 18.096283 60-61 AACGCAG 17190 0.0 17.969465 6 GTGTAGC 1090 0.0 17.909792 1 >>END_MODULE