FastQCFastQC Report
Fri 27 May 2016
SRR939150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224723
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64280.28893484716973755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55300.24857027144502936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51110.22973646606791048No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31770.14280429518641197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.13799470765573962No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23410.10522658326452326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG75550.023.6349641
TACATGG76050.022.9828172
ACATGGG77900.022.0721823
GAGTACT61100.020.30071812-13
CATGGGG46250.020.2782064
GTATCAA138050.020.089911
TAGTGCG1200.004857919619.7256039
ATTCGCG3600.019.1546980-81
TATTACG1250.00613134718.946812
ATAGCGT1250.00615049818.936586
GTACTTT64800.018.92239414-15
AGAGTAC105050.018.29685610-11
CGCGGAA3650.018.2402282-83
AGTACTT66800.018.00149512-13
GTACCTG19000.017.9961851
TACCTGG17950.017.9440542
ATCAACG153850.017.7029823
TCAACGC153900.017.697234
CAACGCA154050.017.6792035
GGTATCA110300.017.5234851
Produced by FastQC (version 0.11.5)