FastQCFastQC Report
Fri 27 May 2016
SRR939150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224723
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64300.28902474600208655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62510.2809788005068496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56160.25243592123603703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34070.15314266090654882No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27760.12477957930043426No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1151.2914704E-424.7565525
GTACATG75200.023.4502661
TACATGG77400.021.9471862
ACATGGG75150.021.2783363
GAGTACT71100.020.45767212-13
GTAAGCG3200.020.01889494-95
GTATCAA146850.019.1488171
GTACTTT75900.019.13264514-15
ATTCGCG4600.019.1031480-81
AGAGTAC116700.018.90917210-11
CGCGGAA4550.018.87709482-83
AGTACTT76250.018.85810712-13
CATGGGG43750.018.5461944
GGTATCA119200.018.4326841
AATTCGC5500.018.12177778-79
GTGTAGC11650.017.5916441
GGACCGT2704.932572E-717.5740976
GTACACG2806.891114E-717.0218161
CTCGCGC5300.017.01231260-61
TACTTTT85550.016.78033814-15
Produced by FastQC (version 0.11.5)