FastQCFastQC Report
Fri 27 May 2016
SRR939149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510820
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.3696401972264041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.26497319600767877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.16584223480775204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78600.033.940181
GTATCAA123350.033.2487221
ATCAACG150100.026.9869333
TCAACGC151750.026.7247244
TATCAAC152800.026.6340962
CAACGCA152700.026.5584565
GTACATG127400.026.304841
AACGCAG156400.025.982456
TACATGG128850.025.5913352
ACATGGG129200.024.1652363
ACGCAGA176750.022.910597
CGCAGAG177400.022.7999468
GAGTACT83450.022.41817712-13
GCAGAGT188500.020.9782899
CATGGGG54700.020.9602344
TACCTGG22200.020.9141982
GTACTTT89700.020.40877314-15
AGTACTT88350.019.29858812-13
CATGGGA71650.018.5144144
TCGCGCA2352.6031375E-618.1371739
Produced by FastQC (version 0.11.5)