FastQCFastQC Report
Fri 27 May 2016
SRR939149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2510820
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92810.3696401972264041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68120.27130578854716786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44180.1759584518205208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82400.031.9522821
GTATCAA125050.031.2962341
GTACATG127500.025.7284091
ATCAACG155300.025.175513
TCAACGC157200.024.841044
TATCAAC158500.024.7869782
TACATGG129900.024.6556872
CAACGCA159150.024.566495
AACGCAG163400.024.0146336
ACATGGG130900.023.452393
ACGCAGA184350.021.1825967
CGCAGAG186350.020.9297928
GAGTACT91050.020.58487912-13
GTACTTT94300.019.82511314-15
CATGGGG55900.019.6075214
AGTACTT95700.019.55988312-13
GTATAGG9250.019.5589621
GCAGAGT199950.019.3163689
CATGGGA72750.018.7185154
TGCACCG6150.018.5164895
Produced by FastQC (version 0.11.5)