##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2510820 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.528918440987404 34.0 31.0 34.0 31.0 34.0 2 32.735552130379716 34.0 31.0 34.0 31.0 34.0 3 32.829146255008325 34.0 31.0 34.0 31.0 34.0 4 36.27821906787424 37.0 37.0 37.0 35.0 37.0 5 36.23857982651086 37.0 37.0 37.0 35.0 37.0 6 36.274568069395656 37.0 37.0 37.0 35.0 37.0 7 36.22972335730957 37.0 37.0 37.0 35.0 37.0 8 36.2154109016178 37.0 37.0 37.0 35.0 37.0 9 37.96405795716141 39.0 38.0 39.0 35.0 39.0 10-11 37.90870153973602 39.0 38.0 39.0 35.0 39.0 12-13 37.84761133812857 39.0 38.0 39.0 35.0 39.0 14-15 39.230075831799965 41.0 39.0 41.0 36.0 41.0 16-17 39.17891943667806 40.0 39.0 41.0 36.0 41.0 18-19 39.20828932380657 40.5 38.5 41.0 36.0 41.0 20-21 39.271046112425424 41.0 39.0 41.0 36.0 41.0 22-23 39.23340004460694 41.0 39.0 41.0 36.0 41.0 24-25 39.18544977338081 41.0 39.0 41.0 36.0 41.0 26-27 39.06378195171298 40.5 39.0 41.0 35.0 41.0 28-29 38.91995383978143 40.0 38.0 41.0 35.0 41.0 30-31 38.738563098907925 40.0 38.0 41.0 35.0 41.0 32-33 38.6022301479198 40.0 38.0 41.0 35.0 41.0 34-35 38.55916154881672 40.0 38.0 41.0 35.0 41.0 36-37 38.464879601086494 40.0 38.0 41.0 34.0 41.0 38-39 38.34151830875969 40.0 38.0 41.0 34.0 41.0 40-41 38.17282700472356 40.0 38.0 41.0 34.0 41.0 42-43 38.01083928756343 40.0 38.0 41.0 33.0 41.0 44-45 37.79035892656582 40.0 38.0 41.0 33.0 41.0 46-47 37.48655538827953 40.0 37.0 41.0 33.0 41.0 48-49 37.17421101472826 40.0 37.0 41.0 32.0 41.0 50-51 36.931303717510616 40.0 37.0 41.0 31.0 41.0 52-53 36.70403732645112 40.0 36.0 41.0 31.0 41.0 54-55 36.469364789192376 39.0 36.0 41.0 31.0 41.0 56-57 36.21630084992154 39.0 35.0 41.0 30.0 41.0 58-59 35.92877048135669 39.0 35.0 40.0 29.5 41.0 60-61 35.58997558566524 38.0 35.0 40.0 29.0 41.0 62-63 35.21598959702408 38.0 35.0 40.0 28.0 41.0 64-65 34.81465377844688 37.0 34.0 40.0 28.0 41.0 66-67 34.468463689153346 37.0 34.0 39.5 27.5 41.0 68-69 34.01609952127194 36.0 34.0 39.0 26.0 40.5 70-71 33.60400566348842 36.0 33.5 39.0 26.0 40.0 72-73 33.16263511522132 35.0 33.0 38.0 26.0 40.0 74-75 32.68674178156937 35.0 33.0 37.0 25.0 39.0 76-77 30.70202901840833 33.5 30.0 35.5 21.5 37.5 78-79 31.66759405293888 34.5 31.5 36.0 22.5 38.5 80-81 31.701599079185286 35.0 32.0 36.0 23.0 37.5 82-83 31.42655984897364 35.0 32.0 35.5 22.5 37.0 84-85 31.07051262137469 35.0 31.5 35.0 20.5 37.0 86-87 30.542645032300207 34.0 31.0 35.0 18.5 36.0 88-89 30.028422985319537 34.0 31.0 35.0 12.5 36.0 90-91 29.60971594937112 34.0 30.0 35.0 7.0 35.5 92-93 29.359039278004794 34.0 30.0 35.0 3.5 35.0 94-95 29.06965752224373 34.0 30.0 35.0 2.0 35.0 96-97 28.57106542882405 34.0 29.0 35.0 2.0 35.0 98-99 27.865405325750153 34.0 29.0 35.0 2.0 35.0 100-101 24.34485765606455 30.0 14.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1389056294495603 1101 2 0.19600081579004325 1101 3 0.1707486130132878 1101 4 0.10492072232312921 1101 5 0.13945594106697712 1101 6 0.13116662262749657 1101 7 0.1354428057558792 1101 8 0.1300039994775659 1101 9 0.2682868418390143 1101 10-11 0.21613792136368914 1101 12-13 0.18749626597391966 1101 14-15 0.3097651380815094 1101 16-17 0.28711702218413393 1101 18-19 0.22537026348082634 1101 20-21 0.35230266473642047 1101 22-23 0.2986808328081949 1101 24-25 0.29343197767633455 1101 26-27 0.20541043485170007 1101 28-29 0.21416993109183124 1101 30-31 0.16052907592167998 1101 32-33 0.18053766766478674 1101 34-35 0.30770683164653434 1101 36-37 0.2957626362319701 1101 38-39 0.24865266758100546 1101 40-41 0.3021315305093779 1101 42-43 0.21885943307699307 1101 44-45 0.19936043521154545 1101 46-47 0.15809221821982788 1101 48-49 0.2258524507186337 1101 50-51 0.21498707445265808 1101 52-53 0.2694869143702334 1101 54-55 0.33659316517776006 1101 56-57 0.3024550120508849 1101 58-59 0.24394898199776804 1101 60-61 0.2464598064675343 1101 62-63 0.3356962682836766 1101 64-65 0.29476856132690443 1101 66-67 0.2638760336420134 1101 68-69 0.2539568541426789 1101 70-71 0.29921890000957774 1101 72-73 0.29839101476274266 1101 74-75 0.31850379623707425 1101 76-77 0.36018009207655766 1101 78-79 0.36376160517989575 1101 80-81 0.4728930296123188 1101 82-83 0.42167324972179543 1101 84-85 0.372463113316055 1101 86-87 0.6149633950022881 1101 88-89 0.62426212934486 1101 90-91 0.5991967729982512 1101 92-93 0.5149815483516242 1101 94-95 0.41519052161788395 1101 96-97 0.4474890323081766 1101 98-99 0.6184548214791299 1101 100-101 0.21211291977595081 1102 1 0.12654364135100593 1102 2 0.1250443675588997 1102 3 0.15466439393946985 1102 4 0.11192963628612063 1102 5 0.07458277264095159 1102 6 0.09119619444216198 1102 7 0.04158941045101017 1102 8 0.07704893894018028 1102 9 0.2081107744055828 1102 10-11 0.18267723127387825 1102 12-13 0.13872966212716165 1102 14-15 0.18646188154601617 1102 16-17 0.17787590584812563 1102 18-19 0.20547215898566407 1102 20-21 0.1438005407771854 1102 22-23 0.1258243348866941 1102 24-25 0.16133974148964114 1102 26-27 0.2406436158821066 1102 28-29 0.06403881221071117 1102 30-31 0.16536479479552924 1102 32-33 0.13616631111838018 1102 34-35 0.15359594037143154 1102 36-37 0.31632693977937265 1102 38-39 0.3515821254714737 1102 40-41 0.40690657565487953 1102 42-43 0.32140182136462414 1102 44-45 0.2518689643773513 1102 46-47 0.3236352973244294 1102 48-49 0.3230883495684864 1102 50-51 0.2207044939482401 1102 52-53 0.19761430041572936 1102 54-55 0.1535365121254415 1102 56-57 0.16574672467102403 1102 58-59 0.11317399442035025 1102 60-61 0.1222896200681518 1102 62-63 0.14615373759080086 1102 64-65 0.16223168745554517 1102 66-67 0.17744513351960478 1102 68-69 0.16527511776813952 1102 70-71 0.2349642097258524 1102 72-73 0.172554005151909 1102 74-75 0.2119452767669472 1102 76-77 0.2171559801399674 1102 78-79 0.14481807037847716 1102 80-81 0.2029400081970003 1102 82-83 0.19193607831434534 1102 84-85 0.18413708708925114 1102 86-87 0.17478534298498616 1102 88-89 0.17846475081351088 1102 90-91 0.43356923545779935 1102 92-93 0.3560459215867091 1102 94-95 0.2881726088547367 1102 96-97 0.36354870919974047 1102 98-99 0.13956147695536814 1102 100-101 -0.2532803308209388 1103 1 0.1007870639872479 1103 2 0.08314752324925223 1103 3 0.09778945411662932 1103 4 0.07746467142958124 1103 5 0.1254881380366797 1103 6 0.10833615448452605 1103 7 0.14668807090739477 1103 8 0.18001431901913634 1103 9 0.16285754890972015 1103 10-11 0.20065102111116317 1103 12-13 0.2133892098356185 1103 14-15 0.23213183388570968 1103 16-17 0.20261059978708573 1103 18-19 0.20751442140623766 1103 20-21 0.17058464317464228 1103 22-23 0.22782048655721354 1103 24-25 0.21578653284339566 1103 26-27 0.19866327576079357 1103 28-29 0.28126971498848263 1103 30-31 0.2422689506303115 1103 32-33 0.22731402498801856 1103 34-35 0.265106853499681 1103 36-37 0.31901347393592516 1103 38-39 0.2660350248071168 1103 40-41 0.3393956986329272 1103 42-43 0.272346527579721 1103 44-45 0.3689575481680336 1103 46-47 0.42838681078004015 1103 48-49 0.5136720529913603 1103 50-51 0.4412703397984288 1103 52-53 0.5021667779094656 1103 54-55 0.4491383890511145 1103 56-57 0.4346953845256394 1103 58-59 0.4733053461197585 1103 60-61 0.512228976533585 1103 62-63 0.46387711521452246 1103 64-65 0.5074785943494433 1103 66-67 0.512797034516133 1103 68-69 0.4987303098536344 1103 70-71 0.4423873634905391 1103 72-73 0.3074858695607219 1103 74-75 0.32160724660614903 1103 76-77 0.2830187551495946 1103 78-79 0.42185303631820403 1103 80-81 0.26684213606143103 1103 82-83 0.2883022804133262 1103 84-85 0.27661885340929615 1103 86-87 0.3093016088717526 1103 88-89 0.37004325793848736 1103 90-91 0.5252754686081964 1103 92-93 0.42741265023585484 1103 94-95 0.42121227763963986 1103 96-97 0.4313920650393328 1103 98-99 0.28194064121494833 1103 100-101 -0.046525143549612125 1104 1 0.04538447943599522 1104 2 0.0455693543341269 1104 3 0.14481522936489455 1104 4 0.03794838184742133 1104 5 0.11017493539474543 1104 6 0.09041732133856328 1104 7 0.09540179701756557 1104 8 0.01477416186155267 1104 9 0.08256899728895917 1104 10-11 0.05553313828841766 1104 12-13 0.07180320060759016 1104 14-15 0.11686781617913056 1104 16-17 0.15434312449666265 1104 18-19 0.13853035381207945 1104 20-21 0.1489147267890374 1104 22-23 0.14100724585551205 1104 24-25 0.14927122695993944 1104 26-27 0.14665679388895114 1104 28-29 0.11968805335742161 1104 30-31 0.08463342856735068 1104 32-33 0.08238497718494386 1104 34-35 0.15539260919107534 1104 36-37 0.11943132631085973 1104 38-39 0.1800426309060441 1104 40-41 0.22677207756772333 1104 42-43 0.20456975984972559 1104 44-45 0.11386156435222006 1104 46-47 0.10691531754882533 1104 48-49 -0.1388330850777848 1104 50-51 -0.07037448641479926 1104 52-53 -0.08738676633355169 1104 54-55 -0.05159316367046074 1104 56-57 -0.013436832192553538 1104 58-59 -0.09672573114743699 1104 60-61 0.028181510143291177 1104 62-63 -0.11377652915496839 1104 64-65 -0.146023782922434 1104 66-67 -0.025089638122985036 1104 68-69 -0.17999984051173357 1104 70-71 -0.08288871623589245 1104 72-73 -0.0536805014107955 1104 74-75 -0.06380466258908513 1104 76-77 -0.10576363957122226 1104 78-79 0.030940758235949772 1104 80-81 0.022843869244866255 1104 82-83 0.004143635575402982 1104 84-85 -0.14100955440408214 1104 86-87 -0.06817739970047754 1104 88-89 0.03287027920511676 1104 90-91 0.11668113307325356 1104 92-93 0.02579166993324833 1104 94-95 -0.05220443586982171 1104 96-97 0.06465432906875179 1104 98-99 0.10934836090765643 1104 100-101 -0.09321423620466263 1105 1 0.04303173932787985 1105 2 0.036809159363869526 1105 3 0.08793356670266661 1105 4 0.065624657031492 1105 5 0.06451244798189748 1105 6 0.010193200769116117 1105 7 0.10410780594742874 1105 8 0.07661748923264611 1105 9 0.13901290780844278 1105 10-11 0.09325932503376322 1105 12-13 0.05960894311978393 1105 14-15 0.14305353110442098 1105 16-17 0.15082494037213934 1105 18-19 0.15354956942561415 1105 20-21 0.17380610598543456 1105 22-23 0.20468614755244374 1105 24-25 0.10866891625398267 1105 26-27 0.1322339800380803 1105 28-29 0.17908305999831953 1105 30-31 0.11672737614653528 1105 32-33 0.17817203919296531 1105 34-35 0.3419783307963513 1105 36-37 0.2536072436894088 1105 38-39 0.2238465371332765 1105 40-41 0.2518738051963467 1105 42-43 0.30876178735988447 1105 44-45 0.24452738109751948 1105 46-47 0.32641799274028926 1105 48-49 0.3255308880629997 1105 50-51 0.346869572485609 1105 52-53 0.2661162989974031 1105 54-55 0.3447673287722779 1105 56-57 0.36214882119159597 1105 58-59 0.3772098951259366 1105 60-61 0.3766282184390448 1105 62-63 0.3625725078258455 1105 64-65 0.21315069541701348 1105 66-67 0.2281930587997394 1105 68-69 0.20375493585448368 1105 70-71 0.26370509596701197 1105 72-73 0.2682244630253905 1105 74-75 0.21363337167638718 1105 76-77 0.25659875453503744 1105 78-79 0.4556317556783682 1105 80-81 0.28211376831314894 1105 82-83 0.30378790866167904 1105 84-85 0.44561313807006186 1105 86-87 0.5045202798578465 1105 88-89 0.6759049923815255 1105 90-91 0.6542892653141941 1105 92-93 0.5460605357359221 1105 94-95 0.27120876591046184 1105 96-97 0.3433505454263255 1105 98-99 0.3147928676512457 1105 100-101 0.11021979078104849 1106 1 0.048157684236230125 1106 2 0.04582824665495622 1106 3 0.058935344348320484 1106 4 0.0226391929143972 1106 5 0.005038228505064524 1106 6 -0.010630130394162052 1106 7 -0.014595833400953495 1106 8 -0.01716320243154712 1106 9 -0.004708111793995329 1106 10-11 0.05478452670426748 1106 12-13 0.027292287752231914 1106 14-15 -0.06827335353747799 1106 16-17 -0.05850280503903349 1106 18-19 -0.09899471504740376 1106 20-21 0.015420157137505441 1106 22-23 0.05378574939917513 1106 24-25 0.048041997930560854 1106 26-27 0.08948830904633098 1106 28-29 0.06854516206732342 1106 30-31 0.11309172220616404 1106 32-33 0.13661937784318212 1106 34-35 0.03678806125265055 1106 36-37 0.12334864864233452 1106 38-39 0.19164451289260143 1106 40-41 0.1727292986773037 1106 42-43 0.12308266785124289 1106 44-45 0.050907380175630124 1106 46-47 0.2247807529542456 1106 48-49 0.40093089286231276 1106 50-51 0.41704479755708945 1106 52-53 0.4820648551717426 1106 54-55 0.3570008351399139 1106 56-57 0.38712364636618446 1106 58-59 0.30999185964449794 1106 60-61 0.2996352898330059 1106 62-63 0.3696164524624663 1106 64-65 0.549859691008372 1106 66-67 0.5409689223182639 1106 68-69 0.22510291158790352 1106 70-71 0.2574353430298899 1106 72-73 0.2028754846148928 1106 74-75 0.1170111573724526 1106 76-77 0.18187721408604673 1106 78-79 0.43472193325820996 1106 80-81 0.39751632469086573 1106 82-83 0.42255999867542116 1106 84-85 0.3218498465132136 1106 86-87 0.5075292606196733 1106 88-89 0.5763257611247958 1106 90-91 0.5887592767583136 1106 92-93 0.6471613053009442 1106 94-95 0.5267416599510817 1106 96-97 0.45338123514834905 1106 98-99 0.50196089702996 1106 100-101 0.08854622121437217 1107 1 0.07799485347854329 1107 2 0.025498175859688388 1107 3 0.02366587647271956 1107 4 0.0800144538927654 1107 5 0.08160636092824092 1107 6 0.1346434670681944 1107 7 0.037090304843133026 1107 8 0.07742374840334065 1107 9 0.1464586198601907 1107 10-11 0.1263606303815763 1107 12-13 0.013745844708438426 1107 14-15 0.18488660296201687 1107 16-17 0.15107372311372558 1107 18-19 0.06426956698856401 1107 20-21 0.07659932690958726 1107 22-23 0.1118843461623058 1107 24-25 0.06404414663187907 1107 26-27 0.07955245748770068 1107 28-29 0.09636812947978513 1107 30-31 0.1425670178993883 1107 32-33 0.19030400574437323 1107 34-35 0.1453983319133343 1107 36-37 0.21727683989978175 1107 38-39 0.19538680693562327 1107 40-41 0.21325206158473264 1107 42-43 0.21690967600675748 1107 44-45 0.20553144793376532 1107 46-47 0.3158800127546044 1107 48-49 0.29205590088292155 1107 50-51 0.23491784649186087 1107 52-53 0.17639128008483596 1107 54-55 0.16727708968699062 1107 56-57 0.18043511427797654 1107 58-59 0.18584874889753422 1107 60-61 0.17133676184148072 1107 62-63 0.216102684156958 1107 64-65 0.22200126837888945 1107 66-67 0.18362566152416804 1107 68-69 0.09699210704238936 1107 70-71 0.20591438825626796 1107 72-73 0.16374789683649738 1107 74-75 0.28580727588995813 1107 76-77 0.3665429561201421 1107 78-79 0.3837218658028121 1107 80-81 0.29461154703867365 1107 82-83 0.16843740424197406 1107 84-85 0.26925816063821273 1107 86-87 0.34000253758661714 1107 88-89 0.39460609261050905 1107 90-91 0.5439114709508992 1107 92-93 0.5956977698811023 1107 94-95 0.6404559188712327 1107 96-97 0.6820608861571387 1107 98-99 0.7616929839190973 1107 100-101 0.4585430458747517 1108 1 0.013385043559487997 1108 2 -0.03041462911467363 1108 3 0.033918567810182765 1108 4 0.04867192046944524 1108 5 0.05384717160838903 1108 6 0.07972342934878185 1108 7 0.002277872754831378 1108 8 0.061798912947089946 1108 9 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-0.18382759276721572 2316 74-75 -0.40510956317004343 2316 76-77 -0.3021670883086536 2316 78-79 -0.26981649888143266 2316 80-81 -0.21274774732910018 2316 82-83 -0.2437160251514534 2316 84-85 -0.35254729432678644 2316 86-87 -0.4991278131087604 2316 88-89 -0.7453568144811591 2316 90-91 -0.7562917997873164 2316 92-93 -0.8388267779948926 2316 94-95 -0.6046327719931881 2316 96-97 -0.8899549086727951 2316 98-99 -0.8560579934210182 2316 100-101 -0.44655979658109146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 16.0 5 53.0 6 88.0 7 239.0 8 464.0 9 939.0 10 1609.0 11 2709.0 12 4134.0 13 6053.0 14 7466.0 15 10096.0 16 14059.0 17 20645.0 18 20091.0 19 12443.0 20 10588.0 21 11206.0 22 11504.0 23 12682.0 24 14623.0 25 16997.0 26 20654.0 27 25085.0 28 30818.0 29 37945.0 30 47118.0 31 58040.0 32 72931.0 33 95687.0 34 133530.0 35 198734.0 36 332520.0 37 605620.0 38 606780.0 39 66642.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49656028798221 19.307069784209176 14.340519699660165 22.855850228148448 2 15.633219426322874 20.527357596323114 40.44714475748958 23.392278219864426 3 19.68321106252141 24.911901291211635 29.228618538963367 26.17626910730359 4 13.181948526776113 16.62512645271266 36.47489664731044 33.71802837320079 5 14.080499597740978 37.33788164822647 33.43600895325034 15.145609800782214 6 32.140177312591106 35.60960164408441 17.568762396348603 14.681458646975889 7 29.15653053584088 32.61667503046814 20.30073840418668 17.926056029504306 8 26.17188806843979 35.46423080905839 19.62024358576083 18.743637536740984 9 26.810723190033535 17.33800113110458 20.396364534295568 35.45491114456632 10-11 25.229407125958854 26.79702248667766 26.77404194645574 21.19952844090775 12-13 26.104240845620158 24.051286034044654 27.721441600751945 22.123031519583243 14-15 22.947838769599393 25.343716245710517 26.17227950383783 25.536165480852258 16-17 22.28149999721202 28.833512958931458 26.638635514569586 22.246351529286937 18-19 22.119794377850415 27.13716122109957 29.30028010780546 21.442764293244554 20-21 22.901283246110832 26.29710612469233 29.24626217729666 21.555348451900176 22-23 22.53251925665719 26.31269864028485 29.071279502313985 22.08350260074398 24-25 22.318552434116825 26.548346189752465 29.243414868839036 21.889686507291678 26-27 22.158298309600834 26.72901737396186 29.185647189787307 21.92703712665 28-29 21.963581618754034 26.732481818688715 29.461132219752912 21.842804342804342 30-31 22.120017014876865 26.601039373189778 29.288350385383776 21.99059322654958 32-33 21.89430146326698 26.738814411228205 29.16879744465951 22.198086680845304 34-35 22.409055911759072 26.75301055811384 28.96447077389656 21.87346275623053 36-37 22.413799285224563 26.536127791108054 28.93748961719158 22.112583306475806 38-39 22.204736485679003 26.678763494403057 29.118949926303628 21.99755009361431 40-41 22.329982810221136 26.526750510405584 29.161301707008175 21.981964972365105 42-43 22.226349199647412 26.6944982057938 28.772124858747837 22.30702773581095 44-45 22.185062048130987 26.945985317150935 28.682874954819322 22.186077679898755 46-47 22.324155550152213 27.0947428330867 28.150804238424413 22.43029737833667 48-49 22.4768241567243 27.08526830449529 28.042053461759657 22.395854077020747 50-51 22.453673971256784 27.280964354850816 27.88409437002316 22.381267303869237 52-53 22.67303378282173 27.279003750770414 27.757811947702294 22.290150518705563 54-55 22.30366971746282 27.135776359914292 27.911240152619467 22.649313770003427 56-57 22.363988657092104 27.541679610645126 27.702623047450636 22.391708684812134 58-59 22.415762927582914 27.12894056760065 27.908017858575995 22.54727864624044 60-61 22.461956507668248 27.061729349048957 28.120727457255402 22.355586686027394 62-63 22.3861238238187 27.120442120100584 27.98696629115029 22.506467764930424 64-65 22.169325866862092 26.975999490198266 27.98594460685524 22.868730036084404 66-67 22.130108886419936 27.36558669760315 27.737808303582757 22.766496112394158 68-69 22.117078490311435 27.187370431780554 28.019175996906093 22.67637508100192 70-71 22.133335776095368 27.18640810030512 27.838127718485485 22.84212840511403 72-73 22.24028405062888 27.097521925108133 27.712141850072886 22.950052174190105 74-75 22.11665909941772 27.339355270389753 27.513342254721568 23.03064337547096 76-77 22.1921333813105 27.182756479761277 27.70316104461697 22.921949094311252 78-79 22.086237024867447 27.17046772708038 27.73787369626366 23.005421551788515 80-81 21.916024924872897 27.50709450780545 27.63297347998303 22.94390708733862 82-83 22.150423718265934 27.36046072827168 27.42113841861632 23.06797713484606 84-85 22.045507045507044 27.18456520180658 27.35586382138106 23.41406393130531 86-87 22.005399276109458 27.10229678555281 27.641646272856818 23.250657665480915 88-89 22.094231352944067 27.014351533590954 27.46792829951903 23.423488813945944 90-91 21.9394623989124 27.233163729420113 27.58117496526285 23.24619890640463 92-93 21.922360025808302 27.055543607267747 27.49440421854215 23.527692148381803 94-95 21.802000939931972 26.947590826901173 27.49739129049474 23.753016942672115 96-97 21.845532535187708 26.83832373487546 27.496156634087672 23.819987095849164 98-99 21.666451926774428 26.90546140964824 27.31746329192471 24.110623371652622 100-101 21.81558358954302 26.470312368381173 27.795088540452124 23.919015501623687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 583.0 1 410.0 2 295.5 3 387.0 4 355.0 5 329.0 6 596.0 7 1266.0 8 2145.5 9 2543.0 10 2364.5 11 2221.0 12 2364.5 13 2624.5 14 2865.0 15 3104.5 16 3275.0 17 3489.0 18 3720.0 19 3981.5 20 4385.5 21 4954.5 22 5856.0 23 7062.5 24 8553.0 25 10556.0 26 13334.0 27 16665.0 28 19865.5 29 23430.5 30 27645.5 31 32294.5 32 37697.0 33 42538.0 34 47869.0 35 53095.0 36 58214.0 37 63854.5 38 67808.5 39 69970.5 40 71682.0 41 76131.0 42 81299.5 43 85594.0 44 89441.0 45 93120.5 46 93659.0 47 94119.0 48 103767.0 49 106807.5 50 101010.5 51 96921.0 52 91621.0 53 89077.5 54 86018.0 55 78569.0 56 69419.5 57 60815.0 58 54710.0 59 48333.0 60 40815.0 61 33220.5 62 26711.0 63 21012.0 64 15943.0 65 12100.5 66 8874.0 67 7121.5 68 5192.5 69 3290.0 70 2388.5 71 1861.0 72 1535.5 73 1294.5 74 1007.0 75 813.0 76 582.0 77 287.5 78 143.5 79 89.5 80 55.5 81 40.0 82 26.5 83 10.0 84 2.5 85 3.0 86 2.5 87 0.5 88 2.5 89 2.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34733672664707144 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011151735289666323 16-17 0.0016727602934499485 18-19 3.9827626034522585E-5 20-21 0.0 22-23 0.0 24-25 4.77931512414271E-4 26-27 8.961215857767581E-4 28-29 0.0 30-31 0.0030268995786237166 32-33 0.0 34-35 0.009518802622250898 36-37 0.02632606080881943 38-39 0.020710365537951747 40-41 0.0126054436399264 42-43 0.009518802622250898 44-45 0.0026883647573302744 46-47 0.0011350873419838938 48-49 6.372420165523614E-4 50-51 5.974143905178388E-5 52-53 9.956906508630647E-5 54-55 0.0 56-57 0.0 58-59 0.0037238830342278617 60-61 0.00953871643526816 62-63 0.0039628487904349976 64-65 0.0015931050413809035 66-67 0.003365434399917158 68-69 0.0030667272046582396 70-71 3.9827626034522585E-5 72-73 0.0 74-75 0.0 76-77 0.2175185795875451 78-79 2.987071952589194E-4 80-81 0.0029870719525891937 82-83 4.978453254315323E-4 84-85 0.0 86-87 0.10777355604941813 88-89 0.408272994479891 90-91 0.39409435961160094 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001214742594052939 100-101 7.368110816386678E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2510820.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.58845909059825 #Duplication Level Percentage of deduplicated Percentage of total 1 75.27212369950303 39.58444997833765 2 13.706427974179904 14.415998535967825 3 4.590442093044534 7.24212828653498 4 2.1066407139361103 4.431399560136713 5 1.161646307033212 3.05445946475803 6 0.6854919852838267 2.1629380335018893 7 0.4275274003056361 1.5738105040757935 8 0.30939244914346775 1.3016377723777002 9 0.2384811670299005 1.1287221386607043 >10 1.3233913382739861 12.918571510266924 >50 0.11154943582116239 4.0224425601101 >100 0.06309543222022086 5.867743659904048 >500 0.002956203295486855 1.0093880057429798 >1k 6.822007604957562E-4 0.6448092718140453 >5k 1.5160016899905693E-4 0.6415007178105485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9281 0.3696401972264041 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6812 0.27130578854716786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4418 0.1759584518205208 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.9827626034522585E-5 0.0 0.0 0.0 0.0 9 3.9827626034522585E-5 0.0 0.0 0.0 0.0 10-11 3.9827626034522585E-5 0.0 0.0 0.0 0.0 12-13 3.9827626034522585E-5 0.0 0.0 0.0 0.0 14-15 3.9827626034522585E-5 0.0 0.0 0.0 0.0 16-17 3.9827626034522585E-5 0.0 0.0 0.0 0.0 18-19 3.9827626034522585E-5 0.0 0.0 1.9913813017261293E-5 0.0 20-21 3.9827626034522585E-5 0.0 0.0 5.974143905178388E-5 0.0 22-23 3.9827626034522585E-5 0.0 0.0 2.389657562071355E-4 0.0 24-25 3.9827626034522585E-5 0.0 0.0 4.978453254315323E-4 0.0 26-27 3.9827626034522585E-5 0.0 0.0 0.0011350873419838938 0.0 28-29 3.9827626034522585E-5 0.0 0.0 0.0037238830342278617 0.0 30-31 3.9827626034522585E-5 0.0 0.0 0.01047466564707944 0.0 32-33 3.9827626034522585E-5 0.0 0.0 0.02491218008459388 0.0 34-35 3.9827626034522585E-5 0.0 0.0 0.04586151137875276 0.0 36-37 3.9827626034522585E-5 0.0 0.0 0.08178603006189214 0.0 38-39 3.9827626034522585E-5 0.0 0.0 0.1342190997363411 0.0 40-41 3.9827626034522585E-5 0.0 0.0 0.19228777849467504 0.0 42-43 3.9827626034522585E-5 0.0 0.0 0.2563106873451701 0.0 44-45 3.9827626034522585E-5 0.0 0.0 0.33186369393265946 0.0 46-47 3.9827626034522585E-5 0.0 0.0 0.4247218040321489 0.0 48-49 3.9827626034522585E-5 0.0 0.0 0.528154148843804 3.9827626034522585E-5 50-51 3.9827626034522585E-5 0.0 0.0 0.639731243179519 3.9827626034522585E-5 52-53 3.9827626034522585E-5 0.0 0.0 0.7530806668737703 3.9827626034522585E-5 54-55 3.9827626034522585E-5 0.0 0.0 0.8844520913486431 3.9827626034522585E-5 56-57 3.9827626034522585E-5 0.0 0.0 1.0383858659720728 3.9827626034522585E-5 58-59 3.9827626034522585E-5 0.0 0.0 1.2114169872790563 3.9827626034522585E-5 60-61 3.9827626034522585E-5 0.0 0.0 1.4048000254896806 3.9827626034522585E-5 62-63 3.9827626034522585E-5 0.0 0.0 1.6002541002541002 3.9827626034522585E-5 64-65 3.9827626034522585E-5 0.0 0.0 1.803175854899993 3.9827626034522585E-5 66-67 3.9827626034522585E-5 0.0 0.0 2.0416835934077313 3.9827626034522585E-5 68-69 3.9827626034522585E-5 0.0 0.0 2.3160561091595575 3.9827626034522585E-5 70-71 3.9827626034522585E-5 0.0 0.0 2.620856931201759 3.9827626034522585E-5 72-73 3.9827626034522585E-5 0.0 0.0 2.9358137978827634 3.9827626034522585E-5 74-75 3.9827626034522585E-5 0.0 0.0 3.2647501613018854 3.9827626034522585E-5 76-77 3.9827626034522585E-5 0.0 0.0 3.6230394851084506 3.9827626034522585E-5 78-79 3.9827626034522585E-5 0.0 0.0 4.023625747763679 3.9827626034522585E-5 80-81 7.965525206904517E-5 0.0 0.0 4.47298093849818 3.9827626034522585E-5 82-83 7.965525206904517E-5 0.0 0.0 4.929525015731912 3.9827626034522585E-5 84-85 7.965525206904517E-5 0.0 0.0 5.403035661656351 3.9827626034522585E-5 86-87 7.965525206904517E-5 0.0 0.0 5.904206593861766 3.9827626034522585E-5 88-89 7.965525206904517E-5 0.0 0.0 6.446917740021188 3.9827626034522585E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8240 0.0 31.952282 1 GTATCAA 12505 0.0 31.296234 1 GTACATG 12750 0.0 25.728409 1 ATCAACG 15530 0.0 25.17551 3 TCAACGC 15720 0.0 24.84104 4 TATCAAC 15850 0.0 24.786978 2 TACATGG 12990 0.0 24.655687 2 CAACGCA 15915 0.0 24.56649 5 AACGCAG 16340 0.0 24.014633 6 ACATGGG 13090 0.0 23.45239 3 ACGCAGA 18435 0.0 21.182596 7 CGCAGAG 18635 0.0 20.929792 8 GAGTACT 9105 0.0 20.584879 12-13 GTACTTT 9430 0.0 19.825113 14-15 CATGGGG 5590 0.0 19.607521 4 AGTACTT 9570 0.0 19.559883 12-13 GTATAGG 925 0.0 19.558962 1 GCAGAGT 19995 0.0 19.316368 9 CATGGGA 7275 0.0 18.718515 4 TGCACCG 615 0.0 18.516489 5 >>END_MODULE