FastQCFastQC Report
Fri 27 May 2016
SRR939148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468182
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90370.36613993619595314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64390.26088027544160036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.17109759328931173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122450.034.7043151
GGTATCA77150.032.00271
ATCAACG150050.028.027523
TATCAAC151600.027.8965932
TCAACGC150600.027.8937174
CAACGCA153050.027.4162565
AACGCAG156950.026.7823056
GTACATG122250.024.9347721
ACGCAGA174900.024.0336367
TACATGG125700.023.8488452
CGCAGAG176350.023.5944428
ACATGGG125750.022.7476583
GCAGAGT186850.021.7872129
GAGTACT83900.021.10178412-13
AGTACTT88800.020.15061812-13
GTACTTT88800.019.75220714-15
CATGGGG53550.019.5452184
CATGGGA72050.018.7992174
TACCTGG21200.018.764732
AGAGTAC170400.018.2091910-11
Produced by FastQC (version 0.11.5)