##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2468182 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10102091336862 33.0 31.0 34.0 30.0 34.0 2 32.205225546576386 34.0 31.0 34.0 30.0 34.0 3 32.26808679424775 34.0 31.0 34.0 30.0 34.0 4 35.71065018706076 37.0 35.0 37.0 35.0 37.0 5 35.74964974219891 37.0 35.0 37.0 35.0 37.0 6 35.830283990402656 37.0 36.0 37.0 35.0 37.0 7 35.7653021535689 37.0 36.0 37.0 35.0 37.0 8 35.7581803124729 37.0 36.0 37.0 35.0 37.0 9 37.43165860540268 39.0 38.0 39.0 35.0 39.0 10-11 37.336112369347155 39.0 37.5 39.0 34.5 39.0 12-13 37.23431234001382 39.0 37.0 39.0 34.0 39.0 14-15 38.525474215434684 40.0 38.0 41.0 34.0 41.0 16-17 38.44089495831345 40.0 38.0 41.0 34.0 41.0 18-19 38.342234284181636 40.0 38.0 41.0 34.0 41.0 20-21 38.3303326091836 40.0 38.0 41.0 34.0 41.0 22-23 38.373005920957205 40.0 38.0 41.0 34.0 41.0 24-25 38.3277065872776 40.0 38.0 41.0 34.0 41.0 26-27 38.21454272821048 40.0 38.0 41.0 34.0 41.0 28-29 38.07344150471886 40.0 38.0 41.0 33.0 41.0 30-31 37.871406970798745 40.0 38.0 41.0 33.0 41.0 32-33 37.67470105527064 40.0 37.5 41.0 33.0 41.0 34-35 37.37332538686369 40.0 37.0 41.0 31.5 41.0 36-37 37.11457542434067 40.0 37.0 41.0 31.0 41.0 38-39 36.93397954445823 40.0 36.5 41.0 30.5 41.0 40-41 36.74198965068216 40.0 36.0 41.0 30.0 41.0 42-43 36.52766327604691 40.0 36.0 41.0 30.0 41.0 44-45 36.24386653820504 39.5 36.0 41.0 30.0 41.0 46-47 36.082490472744716 40.0 36.0 41.0 28.5 41.0 48-49 35.951052029388435 40.0 36.0 41.0 28.0 41.0 50-51 34.82895021517862 38.5 34.5 40.0 25.5 40.5 52-53 34.970414256323075 38.5 34.5 39.5 26.5 40.5 54-55 35.50163116010083 39.0 35.0 41.0 27.0 41.0 56-57 35.421152289417876 39.0 35.0 41.0 26.5 41.0 58-59 35.22331963364128 39.0 35.0 41.0 26.0 41.0 60-61 34.93476311714453 38.5 35.0 41.0 26.0 41.0 62-63 34.55999719631697 38.0 34.5 40.0 25.5 41.0 64-65 34.186495768950586 37.5 34.0 40.0 24.0 41.0 66-67 33.80341218759395 37.0 34.0 40.0 23.0 41.0 68-69 33.395027392631505 36.0 34.0 39.0 22.0 41.0 70-71 32.96878917356986 36.0 33.0 39.0 21.5 41.0 72-73 32.524957438308846 35.0 33.0 38.5 20.0 40.0 74-75 32.07871968112562 35.0 32.0 37.5 18.5 39.5 76-77 31.637737614162972 35.0 32.0 37.0 17.0 39.0 78-79 31.201095381134778 35.0 31.5 36.5 12.0 39.0 80-81 30.750446077315207 35.0 31.0 36.0 7.5 37.5 82-83 30.349621705368566 35.0 31.0 35.5 4.5 37.0 84-85 29.971436668770778 34.0 31.0 35.0 2.0 37.0 86-87 29.555983310793128 34.0 30.0 35.0 2.0 36.0 88-89 29.133195404552826 34.0 29.5 35.0 2.0 36.0 90-91 28.724390664869933 34.0 29.0 35.0 2.0 36.0 92-93 28.302924379158426 34.0 29.0 35.0 2.0 35.0 94-95 27.813450142655604 34.0 28.0 35.0 2.0 35.0 96-97 27.216166190337667 34.0 27.0 35.0 2.0 35.0 98-99 26.489316630621243 33.5 25.0 35.0 2.0 35.0 100-101 23.515671858882367 30.0 12.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12467127738631945 1101 2 0.17295998430588355 1101 3 0.09614733908896511 1101 4 0.17386210333913255 1101 5 0.05469825237729253 1101 6 0.14999056915385012 1101 7 0.03202184561374111 1101 8 0.24359799209141642 1101 9 0.1703270694818002 1101 10-11 0.16183430123555098 1101 12-13 0.26169015718002697 1101 14-15 0.2761903765039193 1101 16-17 0.24025132517916603 1101 18-19 0.4311506214384835 1101 20-21 0.35722015755762726 1101 22-23 0.43386255724571754 1101 24-25 0.37917067375155256 1101 26-27 0.3760405314688313 1101 28-29 0.31634108073830447 1101 30-31 0.3154318436506429 1101 32-33 0.4261168194492839 1101 34-35 0.46912604337617836 1101 36-37 0.43620581824135485 1101 38-39 0.3729302008666693 1101 40-41 0.3273624705275111 1101 42-43 0.31554239291406105 1101 44-45 0.21930275780710673 1101 46-47 0.44278286601980454 1101 48-49 0.3650235876387953 1101 50-51 0.3258198987656655 1101 52-53 0.39078964030726127 1101 54-55 0.40292864028013753 1101 56-57 0.4521261633774927 1101 58-59 0.3984468219478856 1101 60-61 0.25576477106174167 1101 62-63 0.23252212463582822 1101 64-65 0.1496091467943117 1101 66-67 0.32319683955678613 1101 68-69 0.24529466218017149 1101 70-71 0.09129183709896438 1101 72-73 0.23443512000938682 1101 74-75 0.07279372752756785 1101 76-77 0.04607169022804669 1101 78-79 -0.11960977459398947 1101 80-81 -0.14724542365848947 1101 82-83 -0.14545079092684787 1101 84-85 -0.08300497119621397 1101 86-87 -0.13729121940485456 1101 88-89 -0.16566440923113745 1101 90-91 -0.22978039419530205 1101 92-93 -0.39002898969213007 1101 94-95 -0.31838189317187826 1101 96-97 -0.30802587468068765 1101 98-99 -0.47642546203865166 1101 100-101 -0.6649130583356921 1102 1 0.18491430445183 1102 2 0.2689457555435055 1102 3 0.29556003932891173 1102 4 0.24222392603804366 1102 5 0.32151142761689044 1102 6 0.3095453039239402 1102 7 0.3304782600276752 1102 8 0.40025255924818737 1102 9 0.40699760603219914 1102 10-11 0.4194496650005419 1102 12-13 0.4447548438378277 1102 14-15 0.4606042156653416 1102 16-17 0.4414703793763195 1102 18-19 0.5404712649821874 1102 20-21 0.5801750587163852 1102 22-23 0.6696968641732184 1102 24-25 0.6775319739345136 1102 26-27 0.7077043651193478 1102 28-29 0.8114419834662456 1102 30-31 0.8548613868655721 1102 32-33 0.8026461991330436 1102 34-35 0.8029351519721359 1102 36-37 0.8137256309847061 1102 38-39 0.7992384093472609 1102 40-41 0.6363104336542094 1102 42-43 0.6277601628649805 1102 44-45 0.592212489911546 1102 46-47 0.7599824989523469 1102 48-49 0.8207476069620796 1102 50-51 0.5852254919723023 1102 52-53 0.6668669620538026 1102 54-55 0.651773594286496 1102 56-57 0.6160361502084655 1102 58-59 0.5909038348058573 1102 60-61 0.5233109994355019 1102 62-63 0.39220626374589784 1102 64-65 0.4974623002188707 1102 66-67 0.5297210634777372 1102 68-69 0.5354931815006125 1102 70-71 0.3521979287368353 1102 72-73 0.3421799493466935 1102 74-75 0.5271147098902063 1102 76-77 0.2673466822066075 1102 78-79 0.24178409384466093 1102 80-81 0.0886754249727204 1102 82-83 -0.09643940742708423 1102 84-85 -0.005185099588178588 1102 86-87 -0.020329188352715022 1102 88-89 0.004996748974445353 1102 90-91 0.1016951500173029 1102 92-93 0.0017752048046340008 1102 94-95 -0.07769492349494911 1102 96-97 -0.19691887974337874 1102 98-99 -0.35129575153698056 1102 100-101 -0.5980234651388514 1103 1 0.21042889022360356 1103 2 0.25946638584047577 1103 3 0.2297890759621808 1103 4 0.2732793265749578 1103 5 0.3255180785974332 1103 6 0.20519212419925736 1103 7 0.2598812900612302 1103 8 0.19051374362263118 1103 9 0.3320264667373394 1103 10-11 0.31777015622135707 1103 12-13 0.3856566918042006 1103 14-15 0.41509402809680296 1103 16-17 0.4675016939423884 1103 18-19 0.39000194305053526 1103 20-21 0.4322594385256693 1103 22-23 0.4325920515454911 1103 24-25 0.5067685291720139 1103 26-27 0.4907564818247536 1103 28-29 0.3514787513236328 1103 30-31 0.43493912851239 1103 32-33 0.4201021590188745 1103 34-35 0.4388366274130959 1103 36-37 0.2973252999578264 1103 38-39 0.35159915894849547 1103 40-41 0.371825604250823 1103 42-43 0.3840245982246202 1103 44-45 0.33532086997078636 1103 46-47 0.40380354415450626 1103 48-49 0.4208150420725616 1103 50-51 0.3584813920938359 1103 52-53 0.45335732806601925 1103 54-55 0.5042290975282668 1103 56-57 0.5905117505952475 1103 58-59 0.4638787786666825 1103 60-61 0.4227300400503893 1103 62-63 0.29738853281819644 1103 64-65 0.2890743899101764 1103 66-67 0.384064229078497 1103 68-69 0.32966458084224115 1103 70-71 0.30661363665127084 1103 72-73 0.1644745136001191 1103 74-75 0.07761824006917095 1103 76-77 0.25046056602835165 1103 78-79 0.10602521952832333 1103 80-81 0.06866079634050237 1103 82-83 -0.020240536539585463 1103 84-85 -0.006075455345943226 1103 86-87 -0.10928895721071186 1103 88-89 -0.03836150350484857 1103 90-91 -0.1027103434907417 1103 92-93 -0.3154937713593462 1103 94-95 -0.19015213314343882 1103 96-97 -0.3205595625624227 1103 98-99 -0.35942661931289166 1103 100-101 -0.526777980507326 1104 1 0.12173127014580132 1104 2 0.16483323767781144 1104 3 0.22016407317929776 1104 4 0.1968986048449466 1104 5 0.18396347745001407 1104 6 0.1117187851936805 1104 7 0.24928348791023325 1104 8 0.24971357016427476 1104 9 0.3769694103604664 1104 10-11 0.28304015402408567 1104 12-13 0.3074680151888458 1104 14-15 0.39642595982466844 1104 16-17 0.42147657151969753 1104 18-19 0.42471839381381926 1104 20-21 0.38974036246244026 1104 22-23 0.4085056359868986 1104 24-25 0.39984374640634 1104 26-27 0.3868174671495197 1104 28-29 0.36534235226875467 1104 30-31 0.5314165300442752 1104 32-33 0.37761192815083433 1104 34-35 0.38949450113502593 1104 36-37 0.3436274524095211 1104 38-39 0.060316796430221586 1104 40-41 0.17172813072209436 1104 42-43 0.39669799608213197 1104 44-45 0.3463106957477393 1104 46-47 0.5591925138680196 1104 48-49 0.5345415799425339 1104 50-51 0.4605539871256852 1104 52-53 0.4732883899854272 1104 54-55 0.4529804780806046 1104 56-57 0.38148204294807186 1104 58-59 0.39300419815615584 1104 60-61 0.4163879689460117 1104 62-63 0.48593350567177396 1104 64-65 0.5220645815758473 1104 66-67 0.4513427429895245 1104 68-69 0.2647217533742534 1104 70-71 0.3762296761285384 1104 72-73 0.3343435777090491 1104 74-75 0.36406574132993796 1104 76-77 0.26077843427163216 1104 78-79 0.32747926903558167 1104 80-81 0.21198047176429924 1104 82-83 0.07419795495350101 1104 84-85 0.06335696220083875 1104 86-87 0.0956684477284 1104 88-89 -0.003405385951968043 1104 90-91 0.16910383537463503 1104 92-93 0.15744072391265362 1104 94-95 0.15815427639444835 1104 96-97 0.08357285043494755 1104 98-99 0.0050186769359754635 1104 100-101 -0.3015473399407185 1105 1 0.04258542156941303 1105 2 0.14128574294414875 1105 3 0.179734804311245 1105 4 0.15801339169411932 1105 5 0.19538705701274495 1105 6 0.2377594746925169 1105 7 0.10417984745516407 1105 8 0.1616697733869259 1105 9 0.23592052877800285 1105 10-11 0.19192087028311988 1105 12-13 0.21423694314089659 1105 14-15 0.11740672455430001 1105 16-17 0.10733725633734537 1105 18-19 0.13630093112973185 1105 20-21 0.09499951428229991 1105 22-23 0.1672513533425004 1105 24-25 0.30890277179720016 1105 26-27 0.3223796799888774 1105 28-29 0.14315304786787664 1105 30-31 0.28136068749446963 1105 32-33 0.4169710657796841 1105 34-35 0.19452576743395866 1105 36-37 0.17746343012410648 1105 38-39 0.2320661794898342 1105 40-41 0.1694447601911122 1105 42-43 0.11907980062583334 1105 44-45 0.14786378210833817 1105 46-47 0.2544493066626714 1105 48-49 0.3686085762583957 1105 50-51 0.09282876933053075 1105 52-53 0.09798710744976802 1105 54-55 0.15291569703684615 1105 56-57 0.11039198900380853 1105 58-59 0.16594608688757972 1105 60-61 0.22373439732655953 1105 62-63 0.17766788680546597 1105 64-65 0.21636431047225102 1105 66-67 0.2834124005807013 1105 68-69 0.14834532667082811 1105 70-71 0.06109100046317195 1105 72-73 0.11005547806971805 1105 74-75 0.2661507141550743 1105 76-77 0.2865589859517925 1105 78-79 0.3033424787473926 1105 80-81 0.3046106374305282 1105 82-83 0.24749139373034623 1105 84-85 0.1903934183552245 1105 86-87 0.16850768200356114 1105 88-89 0.29316757703898233 1105 90-91 0.4232864976451616 1105 92-93 0.2316957419094372 1105 94-95 0.31918787960272255 1105 96-97 0.35288423623325116 1105 98-99 0.14586519838743683 1105 100-101 -0.1752307550720218 1106 1 0.27670906256538075 1106 2 0.2615593648710757 1106 3 0.39117129440731446 1106 4 0.32923333472648153 1106 5 0.3161671611615091 1106 6 0.28292914623683174 1106 7 0.2527149629005834 1106 8 0.28741872905775523 1106 9 0.3429693172775359 1106 10-11 0.3075211482228255 1106 12-13 0.4031249662482068 1106 14-15 0.41249052287786725 1106 16-17 0.42267131835693306 1106 18-19 0.47058482710392724 1106 20-21 0.4906518451549431 1106 22-23 0.4949960169607408 1106 24-25 0.4162475146225333 1106 26-27 0.3765710437700065 1106 28-29 0.39095841071753057 1106 30-31 0.39150028643346246 1106 32-33 0.4149759654679599 1106 34-35 0.3578274131616155 1106 36-37 0.47382665901439225 1106 38-39 0.5021355822089717 1106 40-41 0.6073998837931427 1106 42-43 0.48668062972686954 1106 44-45 0.4932213917982011 1106 46-47 0.4455797735520619 1106 48-49 0.5916908512035377 1106 50-51 0.4213344966543673 1106 52-53 0.3849411869907158 1106 54-55 0.37638006760311526 1106 56-57 0.34530618190812135 1106 58-59 0.3400766942480473 1106 60-61 0.41155450070326083 1106 62-63 0.4504246477354954 1106 64-65 0.336703789385318 1106 66-67 0.2700974940976266 1106 68-69 0.4464985788264144 1106 70-71 0.3729737811041858 1106 72-73 0.4510131798342627 1106 74-75 0.3234346755394668 1106 76-77 0.32194277401703886 1106 78-79 0.31025728357660043 1106 80-81 0.45241560710414674 1106 82-83 0.4884927559066021 1106 84-85 0.5344803545809995 1106 86-87 0.48319925863589575 1106 88-89 0.5506033401168189 1106 90-91 0.6087714927965848 1106 92-93 0.5317105932406392 1106 94-95 0.5712007621942838 1106 96-97 0.6312667207412197 1106 98-99 0.8192013220791914 1106 100-101 0.4534314563144264 1107 1 0.12256945603044755 1107 2 0.2440028437905184 1107 3 0.11631367952868032 1107 4 0.08657812254215713 1107 5 0.12966308684883643 1107 6 0.10764728451506755 1107 7 0.20273977580632874 1107 8 0.20422214181651555 1107 9 0.288962805368314 1107 10-11 0.2823927628412477 1107 12-13 0.30267347905390807 1107 14-15 0.29112840292534514 1107 16-17 0.21022041233720046 1107 18-19 0.37344614087975714 1107 20-21 0.40609809524608664 1107 22-23 0.39917353577962444 1107 24-25 0.27356359679271236 1107 26-27 0.38742507909348234 1107 28-29 0.36728534506934807 1107 30-31 0.4145277398702092 1107 32-33 0.377387635142604 1107 34-35 0.37651909599212274 1107 36-37 0.4097830575045407 1107 38-39 0.39526372255097186 1107 40-41 0.46588440578324253 1107 42-43 0.3644925604357496 1107 44-45 0.32386655735968617 1107 46-47 0.5046307818599303 1107 48-49 0.3487802717608375 1107 50-51 0.24503708003888391 1107 52-53 0.28812204085478754 1107 54-55 0.26104701591229684 1107 56-57 0.20023745342521693 1107 58-59 0.21086289174041184 1107 60-61 0.2659173086379525 1107 62-63 0.29867797276195773 1107 64-65 0.3947448921269938 1107 66-67 0.27535101561475983 1107 68-69 0.3444736656451042 1107 70-71 0.31756376438747225 1107 72-73 0.33630914545528157 1107 74-75 0.2905400536816174 1107 76-77 0.29370399247812173 1107 78-79 0.30750526663426214 1107 80-81 0.3178409458563607 1107 82-83 0.4700268696356851 1107 84-85 0.5351076947487243 1107 86-87 0.42625977476198784 1107 88-89 0.4137831594477923 1107 90-91 0.48501061541364976 1107 92-93 0.439589039484904 1107 94-95 0.7880343105845675 1107 96-97 0.7500222939352312 1107 98-99 0.36124185212028337 1107 100-101 0.40107189992080805 1108 1 0.13719732382534033 1108 2 0.16929142396977426 1108 3 0.20906105608832348 1108 4 0.1847710646716223 1108 5 0.2583056134799193 1108 6 0.3077782564563094 1108 7 0.1915499214691181 1108 8 0.22585077999365666 1108 9 0.2694194882429315 1108 10-11 0.25563937771762113 1108 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-1.3948778112632674 2316 80-81 -1.4407904120248247 2316 82-83 -1.4760122908817301 2316 84-85 -1.4635640694491663 2316 86-87 -1.6310189615760926 2316 88-89 -1.733049656137318 2316 90-91 -1.997868688838789 2316 92-93 -2.0275187936818284 2316 94-95 -1.9237593711334604 2316 96-97 -2.1297632523902905 2316 98-99 -2.6108814900187056 2316 100-101 -2.267672456734047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7273.0 3 1979.0 4 1935.0 5 2278.0 6 2531.0 7 3068.0 8 3891.0 9 4661.0 10 5861.0 11 7200.0 12 9530.0 13 15282.0 14 12910.0 15 13338.0 16 16619.0 17 23812.0 18 19641.0 19 13329.0 20 13410.0 21 14242.0 22 15126.0 23 16673.0 24 18520.0 25 21308.0 26 24111.0 27 28179.0 28 32723.0 29 39035.0 30 46492.0 31 57949.0 32 72746.0 33 94567.0 34 131677.0 35 195977.0 36 321264.0 37 533554.0 38 533234.0 39 92240.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.197229560842075 18.80689294699313 13.962997085567968 23.032880406596824 2 15.276705109650118 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22.986735267586774 26.257876714203544 29.197698911680842 21.55768910652884 22-23 22.599877281256678 26.203371642723805 29.02048867690458 22.176262399114933 24-25 22.3574643858697 26.436165509476144 29.17480918321737 22.031560921436782 26-27 22.159733775013603 26.66458048858345 29.103867534857457 22.07181820154549 28-29 21.991161533201435 26.678039216640588 29.371496269041113 21.959302981116863 30-31 22.11599000658806 26.560973824373956 29.18378822174895 22.139247947289032 32-33 21.90172792373996 26.66909582122558 29.151436293394017 22.277739961640446 34-35 22.453977653635825 26.71845150421042 28.862996695977483 21.964574146176265 36-37 22.439894703075915 26.459711706390625 28.82557269204723 22.27482089848623 38-39 22.19763047709343 26.653528443682596 29.03775345346703 22.111087625756944 40-41 22.384608071638237 26.503138997568726 29.06649251654592 22.045760414247116 42-43 22.27134276094715 26.632649279489257 28.721607095512724 22.374400864050866 44-45 22.24167776569443 26.905285581039195 28.57826545615986 22.274771197106514 46-47 22.305837090775498 26.96145405018698 28.134598736538223 22.5981101224993 48-49 22.539628941866418 27.013116032140612 27.991513131713862 22.4557418942791 50-51 22.4908838189696 27.19439536884766 27.886155929718086 22.428564882464652 52-53 22.63984139445797 27.209811223154617 27.809795541815717 22.340551840571695 54-55 22.251779946083346 27.062898555800967 27.9943639622673 22.690957535848387 56-57 22.3494709171902 27.45501773109926 27.724053240325564 22.471458111384976 58-59 22.3946135414243 26.95209962733592 27.998233881265765 22.655052949974014 60-61 22.446933398439487 26.950366730755537 28.218248034099346 22.38445183670563 62-63 22.36923469737843 26.980132954641938 28.13719954741098 22.513432800568648 64-65 22.198628548105805 26.845328666375153 28.10535442208612 22.85068836343292 66-67 22.112978372976833 27.232902862324643 27.859727638585223 22.7943911261133 68-69 22.112877708481964 27.053915602885137 28.11337152095464 22.719835167678262 70-71 22.04809823029509 27.038122581714962 28.011364539166593 22.902414648823356 72-73 22.201032621326082 26.88416525770418 27.906622907866378 23.00817921310336 74-75 22.082869090709103 27.157205622648657 27.71527448196634 23.044650804675896 76-77 22.182338094584505 26.97654509191722 27.820838697564092 23.02027811593418 78-79 22.165485122560685 26.937088324069503 27.847707237108644 23.04971931626117 80-81 21.982357561722676 27.27940538720237 27.77295469510132 22.965282355973635 82-83 22.12457100548942 27.076105367201382 27.67986410643096 23.11945952087824 84-85 22.05602350062276 26.870906413905065 27.58765748986651 23.485412595605666 86-87 22.04782689435095 26.783582101708607 27.894080545243373 23.274510458697065 88-89 22.155853729562196 26.64073652464144 27.77270940795784 23.430700337838527 90-91 21.9289784056844 26.81310820702941 27.937042412619217 23.320870974666978 92-93 22.031095322869096 26.565969096145775 27.91372504287676 23.48921053810837 94-95 21.962221170784822 26.434935385822417 27.886699767255756 23.716143676137 96-97 22.007915072856964 26.318757867207644 27.822132873827304 23.851194186108092 98-99 21.857616190123718 26.295691203347914 27.706204281808493 24.14048832471987 100-101 22.120670941676458 25.65683984596932 28.260577391049356 23.961911821304863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2365.0 1 1330.0 2 363.5 3 498.0 4 448.5 5 405.5 6 766.5 7 1499.0 8 2385.0 9 2838.0 10 2766.0 11 2778.5 12 2954.0 13 3239.0 14 3555.0 15 3767.5 16 3999.5 17 4227.5 18 4399.5 19 4503.0 20 4775.0 21 5217.0 22 5844.5 23 6901.5 24 8339.5 25 10333.5 26 13156.0 27 16448.0 28 19381.0 29 22824.5 30 26981.5 31 31238.0 32 36428.5 33 41259.0 34 46436.0 35 51571.0 36 56478.5 37 62292.0 38 66252.5 39 67822.0 40 69691.5 41 74236.0 42 79040.5 43 83159.5 44 87124.0 45 90622.5 46 91146.0 47 92086.0 48 100971.0 49 103412.5 50 97889.0 51 94207.5 52 89926.0 53 87362.0 54 84125.5 55 76916.5 56 68352.0 57 60346.5 58 53785.0 59 47287.5 60 40062.0 61 33270.5 62 27189.5 63 21379.0 64 16292.5 65 12493.0 66 9267.5 67 7385.5 68 5576.0 69 3699.0 70 2740.0 71 2175.5 72 1778.0 73 1455.0 74 1101.0 75 889.5 76 669.5 77 387.0 78 231.5 79 145.0 80 98.0 81 68.5 82 46.5 83 41.0 84 37.0 85 23.5 86 13.5 87 15.0 88 19.5 89 14.0 90 8.0 91 7.5 92 7.5 93 6.0 94 4.5 95 4.5 96 3.5 97 2.5 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39409573524156644 2 0.11692816818208707 3 0.11765744989632045 4 0.1296905981811714 5 0.1312707085620104 6 0.07458931310575963 7 0.08386739713684 8 0.08115284853386015 9 0.0807476920259527 10-11 0.07892448774036923 12-13 0.08293553716865289 14-15 0.07667586912148294 16-17 0.07876242513720624 18-19 0.08779741526354215 20-21 0.08635910966047075 22-23 0.09646776453276136 24-25 0.11052669535714951 26-27 0.11135726619835977 28-29 0.1051786294527713 30-31 0.09551564673917888 32-33 0.0933278015964787 34-35 0.34247879613415866 36-37 0.5355966456282397 38-39 0.5166150632327762 40-41 0.4887605533141397 42-43 0.5174253762485911 44-45 0.5271896480891604 46-47 0.5244143260099944 48-49 0.5305524471047921 50-51 0.5295192980096282 52-53 0.5281012502319521 54-55 0.5230975673592952 56-57 0.4937439783614012 58-59 0.4841012534732042 60-61 0.43177529047695834 62-63 0.5077623935349986 64-65 0.5345027230568896 66-67 0.5440846744689006 68-69 0.5594806217693833 70-71 0.555266994087146 72-73 0.5413498680405254 74-75 0.5456242691989488 76-77 0.5216390039308284 78-79 0.4948986744089374 80-81 0.5023535541544343 82-83 0.5158047502169613 84-85 0.5099907543284895 86-87 0.5112264816776072 88-89 0.5213148787245026 90-91 0.5397900154850818 92-93 0.5458268474529026 94-95 0.5405395550247105 96-97 0.5417752823738282 98-99 0.5429704940721551 100-101 0.5196942526928727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2468182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.38168700453222 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75328457404018 41.73973098275244 2 13.051792788134902 14.195570205047265 3 4.25313392435462 6.938777935878322 4 1.9639503897368693 4.272117415483979 5 1.0159845706797561 2.7625477462070265 6 0.6145876719398629 2.005338864736665 7 0.4126174509952251 1.5707183150841173 8 0.29964966499847506 1.3036363434368006 9 0.2178659946337773 1.0663128296194655 >10 1.2532290935766452 12.58482650948626 >50 0.10340887576664744 3.8851473948898505 >100 0.05669178849900287 5.414595792000746 >500 0.003057484674680863 1.0610128636704714 >1k 5.965823755462887E-4 0.5720539485116926 >5k 1.4914559388657219E-4 0.6276128531948785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9037 0.36613993619595314 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6439 0.26088027544160036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4223 0.17109759328931173 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.0515650790743954E-5 0.0 16-17 0.0 0.0 0.0 1.0128912697685989E-4 0.0 18-19 0.0 0.0 0.0 1.2154695237223186E-4 4.0515650790743954E-5 20-21 0.0 0.0 0.0 1.2154695237223186E-4 4.0515650790743954E-5 22-23 0.0 0.0 0.0 3.2412520632595164E-4 4.0515650790743954E-5 24-25 0.0 0.0 0.0 6.279925872565313E-4 4.0515650790743954E-5 26-27 0.0 0.0 0.0 9.92633444373227E-4 4.0515650790743954E-5 28-29 0.0 0.0 0.0 0.0032615098886548885 4.0515650790743954E-5 30-31 0.0 0.0 0.0 0.0110607726658731 4.0515650790743954E-5 32-33 0.0 0.0 0.0 0.023721913537980588 4.0515650790743954E-5 34-35 0.0 0.0 0.0 0.04412154371112017 4.0515650790743954E-5 36-37 0.0 0.0 0.0 0.07934990207367204 4.0515650790743954E-5 38-39 0.0 0.0 0.0 0.13331674892694298 4.0515650790743954E-5 40-41 0.0 0.0 0.0 0.1924695990814292 4.0515650790743954E-5 42-43 0.0 0.0 0.0 0.25579556126736197 4.0515650790743954E-5 44-45 0.0 0.0 0.0 0.3324106569126588 4.0515650790743954E-5 46-47 0.0 0.0 0.0 0.4254548489536023 4.0515650790743954E-5 48-49 0.0 0.0 0.0 0.5302080640730709 4.0515650790743954E-5 50-51 0.0 0.0 0.0 0.6472982948583208 4.0515650790743954E-5 52-53 0.0 0.0 0.0 0.762322227453243 4.0515650790743954E-5 54-55 0.0 0.0 0.0 0.8940791238247423 4.0515650790743954E-5 56-57 0.0 0.0 0.0 1.046499002099521 4.0515650790743954E-5 58-59 0.0 0.0 0.0 1.2166647354206457 4.0515650790743954E-5 60-61 0.0 0.0 0.0 1.4097218114385406 4.0515650790743954E-5 62-63 0.0 0.0 0.0 1.6062227177736488 4.0515650790743954E-5 64-65 0.0 0.0 0.0 1.8115155203303483 4.0515650790743954E-5 66-67 0.0 0.0 0.0 2.054042205963742 4.0515650790743954E-5 68-69 0.0 0.0 0.0 2.3247475267220974 4.0515650790743954E-5 70-71 0.0 0.0 0.0 2.6325246679539838 4.0515650790743954E-5 72-73 0.0 0.0 0.0 2.94599425812197 4.0515650790743954E-5 74-75 0.0 0.0 0.0 3.2773109924632786 4.0515650790743954E-5 76-77 0.0 0.0 0.0 3.631397522548985 4.0515650790743954E-5 78-79 0.0 0.0 0.0 4.0340420601073985 4.0515650790743954E-5 80-81 0.0 0.0 0.0 4.480929688329304 4.0515650790743954E-5 82-83 0.0 0.0 0.0 4.938756542264711 4.0515650790743954E-5 84-85 0.0 0.0 0.0 5.40458523723129 4.0515650790743954E-5 86-87 0.0 0.0 0.0 5.903778570624047 4.0515650790743954E-5 88-89 0.0 0.0 0.0 6.448308917251645 4.0515650790743954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12245 0.0 34.704315 1 GGTATCA 7715 0.0 32.0027 1 ATCAACG 15005 0.0 28.02752 3 TATCAAC 15160 0.0 27.896593 2 TCAACGC 15060 0.0 27.893717 4 CAACGCA 15305 0.0 27.416256 5 AACGCAG 15695 0.0 26.782305 6 GTACATG 12225 0.0 24.934772 1 ACGCAGA 17490 0.0 24.033636 7 TACATGG 12570 0.0 23.848845 2 CGCAGAG 17635 0.0 23.594442 8 ACATGGG 12575 0.0 22.747658 3 GCAGAGT 18685 0.0 21.787212 9 GAGTACT 8390 0.0 21.101784 12-13 AGTACTT 8880 0.0 20.150618 12-13 GTACTTT 8880 0.0 19.752207 14-15 CATGGGG 5355 0.0 19.545218 4 CATGGGA 7205 0.0 18.799217 4 TACCTGG 2120 0.0 18.76473 2 AGAGTAC 17040 0.0 18.20919 10-11 >>END_MODULE