FastQCFastQC Report
Fri 27 May 2016
SRR939148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468182
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.35313441229212433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65230.2642835901080228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.1703277959242876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.035.0647771
GTATCAA121200.032.442821
ATCAACG147750.026.4954643
TCAACGC148200.026.415014
GTACATG124650.026.3447571
CAACGCA150050.026.057715
TATCAAC151200.025.9850522
AACGCAG154900.025.3643636
TACATGG126100.025.2494832
ACATGGG126400.023.9507223
ACGCAGA175350.022.3521527
GAGTACT86950.022.2410812-13
CGCAGAG176600.022.14028
CATGGGG53150.021.9622544
TACCTGG22250.021.7528022
GTACTTT93250.020.40766714-15
GCAGAGT190450.020.4059499
AGTACTT92950.019.96297312-13
CATGGGA70250.019.0479324
AGAGTAC170450.017.7212710-11
Produced by FastQC (version 0.11.5)