##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939148_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2468182 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52886497024936 34.0 31.0 34.0 31.0 34.0 2 32.74506256021638 34.0 31.0 34.0 31.0 34.0 3 32.85342896107338 34.0 31.0 34.0 31.0 34.0 4 36.291360199531475 37.0 37.0 37.0 35.0 37.0 5 36.25383338829957 37.0 37.0 37.0 35.0 37.0 6 36.29154697668162 37.0 37.0 37.0 35.0 37.0 7 36.24652841646199 37.0 37.0 37.0 35.0 37.0 8 36.221528234141566 37.0 37.0 37.0 35.0 37.0 9 37.97629469787884 39.0 38.0 39.0 35.0 39.0 10-11 37.90004444566892 39.0 38.0 39.0 35.0 39.0 12-13 37.81879354926015 39.0 38.0 39.0 35.0 39.0 14-15 39.248052615244745 40.5 39.0 41.0 36.0 41.0 16-17 39.33570376900893 41.0 39.0 41.0 36.0 41.0 18-19 39.369421298753494 41.0 39.0 41.0 36.0 41.0 20-21 39.341637083488976 41.0 39.0 41.0 36.0 41.0 22-23 39.27335646236784 41.0 39.0 41.0 36.0 41.0 24-25 39.20589587801872 41.0 39.0 41.0 36.0 41.0 26-27 39.07029890826527 41.0 39.0 41.0 35.0 41.0 28-29 38.92923009729428 40.0 38.5 41.0 35.0 41.0 30-31 38.74799812169443 40.0 38.0 41.0 35.0 41.0 32-33 38.65894026453479 40.0 38.0 41.0 35.0 41.0 34-35 38.56073903788294 40.0 38.0 41.0 35.0 41.0 36-37 38.42209204993797 40.0 38.0 41.0 34.0 41.0 38-39 38.298927105051405 40.0 38.0 41.0 34.0 41.0 40-41 38.11528525854252 40.0 38.0 41.0 33.5 41.0 42-43 37.929018808175414 40.0 38.0 41.0 33.0 41.0 44-45 37.66736751989926 40.0 37.5 41.0 33.0 41.0 46-47 37.34559404452346 40.0 37.0 41.0 32.5 41.0 48-49 36.999976298344286 40.0 37.0 41.0 31.5 41.0 50-51 36.74723460425528 40.0 36.0 41.0 31.0 41.0 52-53 36.47983758896224 39.0 36.0 41.0 30.5 41.0 54-55 36.24075270786352 39.0 35.0 41.0 30.0 41.0 56-57 36.01782486056538 39.0 35.0 40.0 29.5 41.0 58-59 35.69477331898539 38.5 35.0 40.0 29.0 41.0 60-61 35.3344390729695 38.0 35.0 40.0 28.0 41.0 62-63 34.94215074090971 38.0 34.0 40.0 28.0 41.0 64-65 34.49891073672849 37.0 34.0 40.0 26.5 41.0 66-67 34.08279596075168 36.5 34.0 39.0 26.0 41.0 68-69 33.60476658528423 36.0 33.0 39.0 26.0 40.0 70-71 33.11079733990444 35.5 33.0 38.5 25.0 40.0 72-73 32.54759474787516 35.0 32.0 37.5 23.5 39.5 74-75 32.0145155422088 35.0 31.5 37.0 22.0 39.0 76-77 30.0120637376012 33.0 29.5 35.0 19.5 37.0 78-79 31.0000642173065 34.0 31.0 36.0 20.0 38.0 80-81 31.017025284197032 34.0 31.0 35.5 20.0 37.0 82-83 30.646030154988573 34.0 31.0 35.0 18.0 37.0 84-85 30.19563326367342 34.0 30.5 35.0 14.5 36.5 86-87 29.50512725560757 34.0 29.5 35.0 7.0 36.0 88-89 28.846047819812316 34.0 29.0 35.0 2.0 35.5 90-91 28.312539958560592 33.0 28.5 35.0 2.0 35.0 92-93 27.87218730223298 33.0 27.0 35.0 2.0 35.0 94-95 27.371498941326045 33.0 26.5 34.5 2.0 35.0 96-97 26.60869923692823 32.5 25.5 34.0 2.0 35.0 98-99 25.445164092437267 32.0 22.0 34.0 2.0 35.0 100-101 19.603224559615136 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.026877292448936885 1101 2 0.05520377721148151 1101 3 0.09155662318477908 1101 4 0.06236265643775596 1101 5 0.08054919974539132 1101 6 0.10774035864548637 1101 7 0.10182000882757336 1101 8 0.11495022576583835 1101 9 -9.046591799233283E-5 1101 10-11 0.09759928971255505 1101 12-13 0.06948273890311896 1101 14-15 0.18985539937847307 1101 16-17 0.10754532359720059 1101 18-19 0.06480751314379773 1101 20-21 0.14070312002733232 1101 22-23 0.1180426793633984 1101 24-25 0.10681103837895023 1101 26-27 0.08709575811894155 1101 28-29 0.09337446319564435 1101 30-31 0.12960715350448027 1101 32-33 0.14740877752773685 1101 34-35 0.08391029417955309 1101 36-37 0.1147093564405921 1101 38-39 0.09594857413506475 1101 40-41 0.19494445531301352 1101 42-43 0.1941559314635768 1101 44-45 0.2681304947258738 1101 46-47 0.2616053636799762 1101 48-49 0.33880287456352676 1101 50-51 0.2777288053411766 1101 52-53 0.2868230962686553 1101 54-55 0.16807531497804717 1101 56-57 0.2247939130848664 1101 58-59 0.195976627007326 1101 60-61 0.16983819555549218 1101 62-63 0.03247014295726558 1101 64-65 0.11820974857694466 1101 66-67 0.12188650312219096 1101 68-69 0.25126885258412557 1101 70-71 0.24557057494597956 1101 72-73 0.26415168284442103 1101 74-75 0.12092184320969324 1101 76-77 0.2137208270681228 1101 78-79 0.2361294973838568 1101 80-81 0.3427776475670541 1101 82-83 0.12916566537610308 1101 84-85 0.14085523956341817 1101 86-87 0.4434590928273501 1101 88-89 0.3033833100930856 1101 90-91 0.4241047253924819 1101 92-93 0.2841929371072638 1101 94-95 0.09860216892514728 1101 96-97 -0.05364690102685188 1101 98-99 -0.03241878434651824 1101 100-101 -0.8596755771054987 1102 1 0.07169099510307575 1102 2 0.0968093135945125 1102 3 0.058131931203845966 1102 4 0.10040855293224382 1102 5 0.09807899744191673 1102 6 0.13486526363330853 1102 7 0.1389800775057637 1102 8 0.1495593552624399 1102 9 0.21157033469073383 1102 10-11 0.21519354464971485 1102 12-13 0.1866637776158342 1102 14-15 0.24258593300604758 1102 16-17 0.2509519683671826 1102 18-19 0.2576420233626564 1102 20-21 0.2536408598029638 1102 22-23 0.1638000743928032 1102 24-25 0.2567589296597106 1102 26-27 0.2723615432032389 1102 28-29 0.26904126294058983 1102 30-31 0.2685921091273684 1102 32-33 0.22751037014001696 1102 34-35 0.2718192751673172 1102 36-37 0.24774885619688547 1102 38-39 0.25834632633677757 1102 40-41 0.3447212022103727 1102 42-43 0.32653738769919016 1102 44-45 0.21062322644740306 1102 46-47 0.3503622492563281 1102 48-49 0.2274263552871787 1102 50-51 0.14225470784460725 1102 52-53 0.23855109324459534 1102 54-55 0.21041023055499153 1102 56-57 0.18637011101390044 1102 58-59 -0.0035438421466835734 1102 60-61 -0.018600649259717272 1102 62-63 -0.02932920602052036 1102 64-65 0.02511475599422397 1102 66-67 0.09856473861849224 1102 68-69 0.2112030033283432 1102 70-71 0.16006063612116606 1102 72-73 0.12446714776523038 1102 74-75 0.09058356720207428 1102 76-77 0.14847062765480956 1102 78-79 0.318430453506398 1102 80-81 0.22271889309811144 1102 82-83 0.11385278445993308 1102 84-85 0.1854032959265588 1102 86-87 0.28058403455457537 1102 88-89 0.2446254733744233 1102 90-91 0.44578903513667 1102 92-93 0.48796987015092697 1102 94-95 0.35644490877017176 1102 96-97 0.2525020298973537 1102 98-99 0.3009039353816405 1102 100-101 -0.18808991265707675 1103 1 0.11594601237944602 1103 2 0.08565938773908499 1103 3 0.09120657968806967 1103 4 0.10706534166708082 1103 5 0.1370413499438854 1103 6 0.13284060805908382 1103 7 0.1527869507197508 1103 8 0.15318106331947945 1103 9 0.14397039173118742 1103 10-11 0.18729139145285245 1103 12-13 0.20444916212213826 1103 14-15 0.07635638284850188 1103 16-17 0.22431770339987622 1103 18-19 0.22873805720878693 1103 20-21 0.25301077112589354 1103 22-23 0.2214647045872269 1103 24-25 0.27587078502050844 1103 26-27 0.18243390926834024 1103 28-29 0.2704766874633364 1103 30-31 0.28916732841665294 1103 32-33 0.25781340954679166 1103 34-35 0.33166408028044714 1103 36-37 0.25256044245814735 1103 38-39 0.2889817345164758 1103 40-41 0.38683860459469344 1103 42-43 0.2991771988888914 1103 44-45 0.31584933735422993 1103 46-47 0.39749011840297754 1103 48-49 0.2707656810722625 1103 50-51 0.24069755163147732 1103 52-53 0.2560624913204421 1103 54-55 0.20649000146388374 1103 56-57 0.18271685846650598 1103 58-59 0.15538010418723047 1103 60-61 0.1657012901956918 1103 62-63 0.17020982382717875 1103 64-65 0.2849001573242589 1103 66-67 0.2115192496608742 1103 68-69 0.06324161859131294 1103 70-71 0.11370705903604517 1103 72-73 0.01398442293012181 1103 74-75 0.05501169663582317 1103 76-77 0.09169475095831459 1103 78-79 0.23675105287975384 1103 80-81 0.2630392609449217 1103 82-83 0.11254103436760587 1103 84-85 -0.014138237452119284 1103 86-87 0.09879774316921086 1103 88-89 0.3350144579983372 1103 90-91 0.47568469998237006 1103 92-93 0.40546931136281117 1103 94-95 0.2393704870057185 1103 96-97 -0.0012042975068169426 1103 98-99 -0.13723641777244566 1103 100-101 -0.9640655486941405 1104 1 0.016877479873905088 1104 2 0.07378252381194272 1104 3 0.1430063553150447 1104 4 0.07433398239049183 1104 5 0.07389166757981513 1104 6 0.09156913508596887 1104 7 0.09170734669265102 1104 8 0.10360411004966608 1104 9 0.17176244691262355 1104 10-11 0.16462748927872184 1104 12-13 0.13336801903933093 1104 14-15 0.05359636228904208 1104 16-17 0.178920302758506 1104 18-19 0.15698853804271096 1104 20-21 0.25500157357405584 1104 22-23 0.238985643972633 1104 24-25 0.2255019440262629 1104 26-27 0.18111700027299804 1104 28-29 0.18119519417274432 1104 30-31 0.15023396178550996 1104 32-33 0.078202897118679 1104 34-35 0.09199748427483456 1104 36-37 0.14226822514348214 1104 38-39 0.18632410472035588 1104 40-41 0.09495155773286967 1104 42-43 0.10739198137385841 1104 44-45 0.11506180544785849 1104 46-47 0.0366376944870197 1104 48-49 0.002247114155764507 1104 50-51 -0.10978928363438456 1104 52-53 -0.17547479303026847 1104 54-55 -0.13157084641063221 1104 56-57 -0.07064288080390213 1104 58-59 0.03509922167912549 1104 60-61 -0.01494339023729907 1104 62-63 0.08234629451107622 1104 64-65 0.08003848358664811 1104 66-67 0.061891672105524265 1104 68-69 -0.04548889275268664 1104 70-71 -0.049054471540351585 1104 72-73 -0.346634722729398 1104 74-75 -0.34941894470514256 1104 76-77 -0.2650810144315159 1104 78-79 -0.04011626212623298 1104 80-81 0.11898325867602466 1104 82-83 0.008050960309017086 1104 84-85 -0.11964554022309315 1104 86-87 -0.2497846316421608 1104 88-89 0.01251307623605058 1104 90-91 0.10923659858666923 1104 92-93 -0.061239171157286165 1104 94-95 -0.22204703170736195 1104 96-97 -0.5754573473054343 1104 98-99 -0.6917102785082321 1104 100-101 -0.9344449210885166 1105 1 -0.013201750199236528 1105 2 0.036278630498856046 1105 3 0.0597709153844832 1105 4 0.025532754222993503 1105 5 -0.03888448120095234 1105 6 0.047920165408662285 1105 7 0.06037709606464858 1105 8 0.01305348039266363 1105 9 0.0908617047411795 1105 10-11 0.05554321494619785 1105 12-13 0.1006886087219172 1105 14-15 0.09721722639260122 1105 16-17 0.18021767447269355 1105 18-19 0.10739049382204513 1105 20-21 0.14948375832627647 1105 22-23 0.10009609979896794 1105 24-25 0.08989199778623203 1105 26-27 0.22759302207186494 1105 28-29 0.08128605350296425 1105 30-31 0.19157891946127847 1105 32-33 0.21037253683287105 1105 34-35 0.21105979018511078 1105 36-37 0.18318210782252464 1105 38-39 0.19241591871859498 1105 40-41 0.19634928016775177 1105 42-43 0.23366479526703898 1105 44-45 0.19025776119402593 1105 46-47 0.3662372868179702 1105 48-49 0.19349236615678933 1105 50-51 0.17288708575210165 1105 52-53 0.301328801534396 1105 54-55 0.24393943101441806 1105 56-57 0.23584222692798562 1105 58-59 0.1343954765423092 1105 60-61 0.0935195205417827 1105 62-63 0.16940086724184766 1105 64-65 0.1809726944282133 1105 66-67 0.029337677151630714 1105 68-69 0.18738873097173325 1105 70-71 -0.003382084851885736 1105 72-73 -0.1411548730435399 1105 74-75 -0.06374578396153296 1105 76-77 -0.2840526453104175 1105 78-79 -0.1882353081216941 1105 80-81 0.049416526828999 1105 82-83 -0.10825340554630358 1105 84-85 -0.019623977258316216 1105 86-87 0.005250136334808531 1105 88-89 0.08534751874126201 1105 90-91 0.12698697317406982 1105 92-93 0.18047843878158076 1105 94-95 0.4250078727613307 1105 96-97 -0.03594450444047226 1105 98-99 -0.24378069172466965 1105 100-101 -0.9461330616421897 1106 1 -0.03166654789765744 1106 2 0.021534707838725353 1106 3 0.05722331338300535 1106 4 0.024578051319316785 1106 5 0.03930679465170783 1106 6 0.14097992923854008 1106 7 0.1183735996573887 1106 8 0.0279879147142168 1106 9 0.07797500270535096 1106 10-11 0.059315739018401814 1106 12-13 0.10709353571805735 1106 14-15 0.08682906871186447 1106 16-17 0.061802077393771526 1106 18-19 0.026329300975987735 1106 20-21 0.08633584442896591 1106 22-23 0.08779085150650445 1106 24-25 0.10332119339452106 1106 26-27 0.09718448357509857 1106 28-29 0.09154866292654162 1106 30-31 0.10831415364586405 1106 32-33 0.15458255502976925 1106 34-35 0.057982151142873306 1106 36-37 0.04774341808523985 1106 38-39 0.13268497265484314 1106 40-41 0.17514350934434475 1106 42-43 0.058558575186395956 1106 44-45 0.18906876111574178 1106 46-47 0.20402134310077003 1106 48-49 0.07430480367307268 1106 50-51 0.058307635776785105 1106 52-53 0.10409633191349599 1106 54-55 0.1326619448819244 1106 56-57 0.14375485892343676 1106 58-59 0.11427228019270785 1106 60-61 0.1407979560157031 1106 62-63 -0.027278207107386265 1106 64-65 0.004004875171951028 1106 66-67 0.03707895588163268 1106 68-69 -0.17048813987601363 1106 70-71 -0.18752929853841493 1106 72-73 -0.2636166089729244 1106 74-75 -0.3452362356814831 1106 76-77 -0.25881315642496716 1106 78-79 -0.07738155852693396 1106 80-81 0.15350863153249605 1106 82-83 -0.04598039768886153 1106 84-85 -0.08164999907815584 1106 86-87 -0.17461413929732217 1106 88-89 -0.0978166654953121 1106 90-91 0.0984356016866954 1106 92-93 0.02058437827750481 1106 94-95 -0.08468007414131407 1106 96-97 -0.4548804521323291 1106 98-99 -0.3790095684314565 1106 100-101 -1.124815217776682 1107 1 0.07996116283064225 1107 2 0.02966762097978659 1107 3 0.023451809968072723 1107 4 -0.00936681583409893 1107 5 3.6015413908074834E-4 1107 6 0.05638389764396834 1107 7 0.07338359102273984 1107 8 0.0235713763784986 1107 9 0.1072035640811464 1107 10-11 0.10871972613723102 1107 12-13 0.13223472035732442 1107 14-15 0.11680184071859401 1107 16-17 0.06267781143119322 1107 18-19 0.11888876588581354 1107 20-21 0.15992045396598797 1107 22-23 0.1198056788727726 1107 24-25 0.15475752609118842 1107 26-27 0.13262774658431198 1107 28-29 0.15083544238791546 1107 30-31 0.07171374947178322 1107 32-33 0.022433132435175196 1107 34-35 -0.05206489539752823 1107 36-37 0.054741610830056686 1107 38-39 0.006106493167813198 1107 40-41 0.06800763189520609 1107 42-43 0.03467198261183313 1107 44-45 0.0021050610463149155 1107 46-47 0.044884376743745236 1107 48-49 0.0873518373259401 1107 50-51 0.02840706923750247 1107 52-53 0.11103476536556656 1107 54-55 -0.08248854525903226 1107 56-57 -0.034795050501955416 1107 58-59 0.04197174514842317 1107 60-61 -9.532048769713697E-4 1107 62-63 -0.100913837133632 1107 64-65 -0.058949994998556576 1107 66-67 -0.04862160751520861 1107 68-69 -0.14891600617268352 1107 70-71 -0.11240286394023968 1107 72-73 -0.2468885088117645 1107 74-75 -0.3282184309430747 1107 76-77 -0.1564580223955545 1107 78-79 0.02753183816478355 1107 80-81 0.09768197691066405 1107 82-83 -0.1547208606971502 1107 84-85 -0.2032551283850843 1107 86-87 -0.0842075095289978 1107 88-89 -0.16269672056213125 1107 90-91 0.02519471842139609 1107 92-93 0.10264492106136558 1107 94-95 0.12769686099610666 1107 96-97 -0.05605090452518269 1107 98-99 -0.15050645132672003 1107 100-101 -0.8213743006507279 1108 1 0.02038632753725267 1108 2 -1.6644297886614368E-6 1108 3 0.012881671431493658 1108 4 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-0.9553302292869574 2316 86-87 -1.0613851624138597 2316 88-89 -1.1787792272174649 2316 90-91 -1.308105060353256 2316 92-93 -1.4025108818681566 2316 94-95 -1.1211293163097729 2316 96-97 -1.1546743805371946 2316 98-99 -0.9058726606709868 2316 100-101 0.27334234767139876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 8.0 5 44.0 6 121.0 7 224.0 8 465.0 9 977.0 10 1744.0 11 2826.0 12 4394.0 13 6288.0 14 7812.0 15 10658.0 16 14225.0 17 21117.0 18 21016.0 19 13422.0 20 12368.0 21 13218.0 22 14069.0 23 15146.0 24 17244.0 25 20341.0 26 23783.0 27 28850.0 28 35003.0 29 43012.0 30 52848.0 31 65202.0 32 82283.0 33 108013.0 34 152179.0 35 230303.0 36 383168.0 37 623563.0 38 421768.0 39 20474.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49693818216443 19.312341227583666 14.329527203566839 22.86119338668507 2 15.666875457320408 20.53288614859034 40.3998975764348 23.400340817654453 3 19.640204814717876 24.938558015575836 29.222277773681192 26.19895939602509 4 13.176580981467332 16.61465807626828 36.466759744621754 33.74200119764264 5 14.134897669620797 37.27277810145281 33.45592018740919 15.1364040415172 6 32.19628860432496 35.506619852182695 17.563048429978014 14.73404311351432 7 29.160126765368194 32.56117255534641 20.325851173049635 17.95284950623576 8 26.23157449491164 35.39402685863522 19.661799656589345 18.712598989863793 9 26.81447316283807 17.328381780598026 20.35380697209525 35.50333808446865 10-11 25.237604034062315 26.779082741872358 26.763970404127413 21.219342819937914 12-13 26.11227616115829 24.046849057322355 27.733590958851494 22.10728382266786 14-15 22.926979276629854 25.336167028374025 26.14940590250493 25.587447792491197 16-17 22.2891960446954 28.80905512010416 26.67588012536624 22.22586870983419 18-19 22.12703968488541 27.13740865491456 29.30045865751769 21.43509300268234 20-21 22.906353745388305 26.332377434079014 29.201574276127122 21.55969454440556 22-23 22.560127251556004 26.300309296478137 29.05719675453431 22.082366697431553 24-25 22.35191064835007 26.531568497522773 29.262426309583113 21.854094544544047 26-27 22.191679029467934 26.71696062885888 29.16184765584878 21.929512685824402 28-29 21.940300533247278 26.74973598962312 29.445913475904 21.8640500012256 30-31 22.078679773209593 26.595992515948986 29.27648771443921 22.04883999640221 32-33 21.931038254908476 26.715130176891478 29.14467340307433 22.20915816512572 34-35 22.46579033741648 26.740488761563615 28.904277778002896 21.889443123017013 36-37 22.4417938780481 26.51224062110306 28.88176241276022 22.164203088088623 38-39 22.221927295885028 26.733854488433273 29.03011943098308 22.014098784698618 40-41 22.333913365517724 26.524873818181895 29.12711740617225 22.01409541012814 42-43 22.24588989211986 26.677506882679513 28.762823542663902 22.313779682536726 44-45 22.1887213371938 26.969272645207194 28.61835894418932 22.223647073409683 46-47 22.365545085519997 27.09620576743123 28.076150043242663 22.462099103806104 48-49 22.541036577484814 27.049114238749354 27.996562542162952 22.413286641602884 50-51 22.507857566104338 27.234653309901596 27.849888327855236 22.407600796138826 52-53 22.66317880934226 27.260287126314026 27.730653574169168 22.34588049017455 54-55 22.300827086495243 27.115261354308558 27.89731470369689 22.68659685549931 56-57 22.30813346106575 27.549747678502445 27.716489940666815 22.425628919764982 58-59 22.390194067310244 27.101923170032798 27.898920583265486 22.608962179391472 60-61 22.46919822812577 27.04877516134744 28.12635604170136 22.355670568825435 62-63 22.384966862798922 27.09601026680942 27.996564224788024 22.522458645603635 64-65 22.18186746501018 26.96949751641995 27.956689945608186 22.891945072961683 66-67 22.10238819902955 27.34111896589167 27.789351964397426 22.767140870681356 68-69 22.11416421957665 27.150250145103406 28.004906672933377 22.730678962386563 70-71 22.121430414892924 27.208541195756062 27.808598971144317 22.861429418206704 72-73 22.239060976864753 27.115484190387907 27.673708826982775 22.97174600576457 74-75 22.091320656256304 27.31121529935799 27.482697791329812 23.114766253055894 76-77 22.142478392010748 27.153605555978 27.67315033077333 23.030765721237927 78-79 22.10044276215671 27.104427074521936 27.728281445423026 23.066848717898324 80-81 21.950498132320327 27.454678457050864 27.641578100693653 22.953245309935156 82-83 22.141418258459062 27.334390251610298 27.40369227228786 23.120499217642784 84-85 22.067112554908835 27.12316190621275 27.34109559181616 23.46862994706225 86-87 22.028758195384 27.03346618732961 27.624119925929485 23.313655691356907 88-89 22.030451131567204 27.00287324343674 27.464528982772542 23.502146642223515 90-91 21.898490217408774 27.159993741875983 27.565286617400552 23.376229423314683 92-93 21.88363743030295 26.98289672317519 27.550662795531284 23.582803050990567 94-95 21.80558844639262 26.823229977211966 27.509241115371786 23.861940461023632 96-97 21.826368557910236 26.712211660242236 27.494913260043223 23.966506521804305 98-99 21.634184223635273 26.82375815859422 27.311891213746815 24.230166404023695 100-101 22.03586984464423 25.968190647861668 27.96975172126271 24.026187786231397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 416.5 2 285.5 3 367.5 4 330.0 5 294.5 6 538.5 7 1203.5 8 1993.0 9 2328.0 10 2202.5 11 2057.5 12 2177.0 13 2477.0 14 2754.0 15 3041.5 16 3230.0 17 3445.5 18 3627.0 19 3774.0 20 4197.0 21 4794.5 22 5584.5 23 6786.0 24 8316.0 25 10406.0 26 13368.0 27 16523.5 28 19477.0 29 23129.5 30 27165.5 31 31474.0 32 36957.5 33 41857.0 34 46894.0 35 51725.0 36 56861.5 37 62978.0 38 66995.0 39 68904.0 40 70508.5 41 74722.5 42 79665.5 43 83754.0 44 87643.0 45 91158.5 46 92051.5 47 93362.5 48 102293.5 49 104933.0 50 99156.0 51 94897.5 52 90354.5 53 87939.0 54 84695.5 55 77123.5 56 68071.0 57 60049.5 58 53871.0 59 47346.0 60 40093.5 61 32923.5 62 26575.0 63 20973.0 64 15982.5 65 12115.0 66 8837.5 67 7019.5 68 5269.5 69 3367.0 70 2384.5 71 1852.5 72 1536.0 73 1295.0 74 1032.0 75 841.0 76 578.0 77 289.5 78 159.0 79 97.0 80 51.0 81 35.0 82 21.0 83 9.5 84 5.0 85 1.0 86 1.5 87 3.0 88 1.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43169425917537685 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010736647459547149 16-17 0.0021675873173048017 18-19 2.0257825395371977E-5 20-21 0.0 22-23 0.0 24-25 6.077347618611594E-5 26-27 0.0037476976981438157 28-29 1.0128912697685988E-4 30-31 0.0 32-33 8.103130158148791E-5 34-35 0.012640883046712115 36-37 0.028401471204311513 38-39 0.02116942753816372 40-41 0.008731122745405322 42-43 0.008569060142242348 44-45 0.003565377269585468 46-47 0.005408839380564318 48-49 0.0032007364124687727 50-51 7.900551904195072E-4 52-53 0.0 54-55 0.0 56-57 6.077347618611594E-5 58-59 0.0017624308093973622 60-61 0.0024714546982353815 62-63 0.0013977899522806664 64-65 0.002896869031538193 66-67 0.001863719936374222 68-69 0.004639042015540183 70-71 1.2154695237223188E-4 72-73 0.0 74-75 0.0 76-77 0.09600183454866781 78-79 0.015335173824296586 80-81 5.064456348842994E-4 82-83 0.0 84-85 0.0 86-87 0.10912890540486886 88-89 0.4256776850329514 90-91 0.4285137805883034 92-93 0.0 94-95 2.0257825395371977E-5 96-97 0.0 98-99 1.0128912697685988E-4 100-101 3.443830317213236E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2468182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.93782310323727 #Duplication Level Percentage of deduplicated Percentage of total 1 75.29755926557573 39.86088872506573 2 13.916834398111 14.734538350484955 3 4.492769999143635 7.1351239037459155 4 2.0729953592574284 4.389594464888061 5 1.090254051912499 2.885783806886577 6 0.6886252630719274 2.1872593416553157 7 0.4448413926712521 1.6484254467959918 8 0.3116132525888127 1.3196901793736768 9 0.22344236870697567 1.0645697329540376 >10 1.2846632886165639 12.67518796892156 >50 0.1111537562969674 4.061331019920038 >100 0.06157235959750142 5.813711587468353 >500 0.0028330009300312296 0.9792419841832853 >1k 6.891083343313472E-4 0.6269677971495915 >5k 1.5313518540696608E-4 0.6176856905070226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8716 0.35313441229212433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6523 0.2642835901080228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 0.1703277959242876 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.0515650790743954E-5 0.0 10-11 0.0 0.0 0.0 4.0515650790743954E-5 0.0 12-13 0.0 0.0 0.0 4.0515650790743954E-5 0.0 14-15 0.0 0.0 0.0 1.2154695237223186E-4 0.0 16-17 0.0 0.0 0.0 2.0257825395371979E-4 0.0 18-19 0.0 0.0 0.0 2.4309390474446373E-4 0.0 20-21 0.0 0.0 0.0 2.4309390474446373E-4 0.0 22-23 0.0 0.0 0.0 4.0515650790743957E-4 0.0 24-25 0.0 0.0 0.0 6.887660634426472E-4 0.0 26-27 0.0 0.0 0.0 0.0010736647459547149 0.0 28-29 0.0 0.0 0.0 0.0033425411902363762 0.0 30-31 0.0 0.0 0.0 0.011324124396012936 0.0 32-33 0.0 0.0 0.0 0.024390421776027862 0.0 34-35 0.0 0.0 0.0 0.044992630203121164 0.0 36-37 0.0 0.0 0.0 0.08032227769264991 0.0 38-39 0.0 0.0 0.0 0.13507917973634037 0.0 40-41 0.0 0.0 0.0 0.19542724158915348 0.0 42-43 0.0 0.0 0.0 0.2594824854893197 0.0 44-45 0.0 0.0 0.0 0.3368066050234545 0.0 46-47 0.0 0.0 0.0 0.43088394615956194 0.0 48-49 0.0 0.0 0.0 0.5367918573265666 0.0 50-51 0.0 0.0 0.0 0.6552191045879112 0.0 52-53 0.0 0.0 0.0 0.7715597958335325 0.0 54-55 0.0 0.0 0.0 0.9044916460779635 0.0 56-57 0.0 0.0 0.0 1.0580257047494876 0.0 58-59 0.0 0.0 0.0 1.2296094858482882 0.0 60-61 0.0 0.0 0.0 1.424874664834279 0.0 62-63 0.0 0.0 0.0 1.6240090884707854 4.0515650790743954E-5 64-65 0.0 0.0 0.0 1.8316112831225575 4.0515650790743954E-5 66-67 0.0 0.0 0.0 2.0767512282319536 4.0515650790743954E-5 68-69 0.0 0.0 0.0 2.350231871069475 4.0515650790743954E-5 70-71 0.0 0.0 0.0 2.6615338739201566 4.0515650790743954E-5 72-73 0.0 0.0 0.0 2.9787106461354957 4.0515650790743954E-5 74-75 0.0 0.0 0.0 3.3133091481908545 4.0515650790743954E-5 76-77 0.0 0.0 0.0 3.6700697112287504 4.0515650790743954E-5 78-79 0.0 0.0 0.0 4.074841320453678 4.0515650790743954E-5 80-81 0.0 0.0 0.0 4.523268543405632 4.0515650790743954E-5 82-83 0.0 0.0 0.0 4.982918601626622 4.0515650790743954E-5 84-85 0.0 0.0 0.0 5.451239009116832 4.0515650790743954E-5 86-87 0.0 0.0 0.0 5.952721476779265 4.0515650790743954E-5 88-89 0.0 0.0 0.0 6.497636722089376 4.0515650790743954E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7870 0.0 35.064777 1 GTATCAA 12120 0.0 32.44282 1 ATCAACG 14775 0.0 26.495464 3 TCAACGC 14820 0.0 26.41501 4 GTACATG 12465 0.0 26.344757 1 CAACGCA 15005 0.0 26.05771 5 TATCAAC 15120 0.0 25.985052 2 AACGCAG 15490 0.0 25.364363 6 TACATGG 12610 0.0 25.249483 2 ACATGGG 12640 0.0 23.950722 3 ACGCAGA 17535 0.0 22.352152 7 GAGTACT 8695 0.0 22.24108 12-13 CGCAGAG 17660 0.0 22.1402 8 CATGGGG 5315 0.0 21.962254 4 TACCTGG 2225 0.0 21.752802 2 GTACTTT 9325 0.0 20.407667 14-15 GCAGAGT 19045 0.0 20.405949 9 AGTACTT 9295 0.0 19.962973 12-13 CATGGGA 7025 0.0 19.047932 4 AGAGTAC 17045 0.0 17.72127 10-11 >>END_MODULE