FastQCFastQC Report
Fri 27 May 2016
SRR939147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820764
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33490.40803446544926436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24920.3036195544638898No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17230.2099263612926493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16500.20103220901501526No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11160.13597087591561033No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10100.12305607945767602No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8640.105267774902408No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT1050.002207514822.5981431
GTATCAA67600.022.183621
TACATGG46800.022.0678632
GTACATG47350.021.9490721
GAGTACT29300.021.81877512-13
ACATGGG47450.021.3661963
AGTACTT29600.021.11769312-13
GTACTTT30650.020.31823514-15
TAGTACA2108.821753E-720.303594
GGTATCA51450.020.0154971
AGTACAT36700.019.6212712
GTCCTAC2201.3557383E-619.4138571
TATCAAC77000.019.3191742
CATGGGG20650.019.2712064
TCAACGC78100.018.9864144
GTACACG1751.0003048E-418.9824391
GTATAGA2752.8645445E-818.9824391
ATCAACG78300.018.8774133
CAACGCA78900.018.7939035
GTATATA3306.039045E-1018.6948261
Produced by FastQC (version 0.11.5)