##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 820764 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12226413439186 33.0 31.0 34.0 30.0 34.0 2 32.21342797686059 34.0 31.0 34.0 30.0 34.0 3 32.268892154139316 34.0 31.0 34.0 30.0 34.0 4 35.71214015234586 37.0 35.0 37.0 35.0 37.0 5 35.76149051371649 37.0 35.0 37.0 35.0 37.0 6 35.84747990896287 37.0 36.0 37.0 35.0 37.0 7 35.77148120531602 37.0 36.0 37.0 35.0 37.0 8 35.765247988459535 37.0 36.0 37.0 35.0 37.0 9 37.45081655628171 39.0 38.0 39.0 35.0 39.0 10-11 37.36339374046619 39.0 37.5 39.0 34.5 39.0 12-13 37.273970349576736 39.0 37.0 39.0 34.0 39.0 14-15 38.58245488349879 40.0 38.0 41.0 34.0 41.0 16-17 38.52376444385962 40.0 38.0 41.0 34.0 41.0 18-19 38.46567588247048 40.0 38.0 41.0 34.0 41.0 20-21 38.37152214278404 40.0 38.0 41.0 34.0 41.0 22-23 38.29098924904113 40.0 38.0 41.0 34.0 41.0 24-25 38.319352456979104 40.0 38.0 41.0 34.0 41.0 26-27 38.23486044709563 40.0 38.0 41.0 34.0 41.0 28-29 38.11690632142735 40.0 38.0 41.0 33.5 41.0 30-31 37.95022808017896 40.0 38.0 41.0 33.0 41.0 32-33 37.74157553206524 40.0 37.5 41.0 33.0 41.0 34-35 37.4338774605124 40.0 37.0 41.0 32.5 41.0 36-37 37.18517685960885 40.0 37.0 41.0 31.0 41.0 38-39 36.94564515500193 40.0 36.5 41.0 30.5 41.0 40-41 36.789381600557526 40.0 36.0 41.0 30.5 41.0 42-43 36.59018792247223 40.0 36.0 41.0 30.0 41.0 44-45 36.304105686896605 39.5 35.5 41.0 30.0 41.0 46-47 35.90752640223012 39.0 35.0 41.0 28.5 41.0 48-49 35.87234698402951 39.0 35.0 41.0 28.0 41.0 50-51 34.85749435891438 38.0 34.0 40.0 26.0 40.5 52-53 35.01027335506918 38.5 34.5 39.5 26.5 40.5 54-55 35.5064037896399 39.0 35.0 41.0 27.0 41.0 56-57 35.39345902110716 39.0 35.0 41.0 27.0 41.0 58-59 35.195782831605676 39.0 35.0 41.0 26.5 41.0 60-61 34.90107509588627 38.0 35.0 40.5 26.0 41.0 62-63 34.529984258568845 38.0 34.5 40.0 26.0 41.0 64-65 34.14694784371634 37.0 34.0 40.0 25.0 41.0 66-67 33.75106790746183 36.5 34.0 39.5 23.5 41.0 68-69 33.34386437514316 36.0 33.5 39.0 22.5 41.0 70-71 32.93122627210745 35.5 33.0 39.0 22.0 40.5 72-73 32.48381264285471 35.0 33.0 38.0 20.5 40.0 74-75 32.0380279836835 35.0 32.5 37.0 19.5 39.5 76-77 31.609589358207717 35.0 32.0 37.0 18.0 39.0 78-79 31.201251516879395 35.0 32.0 36.0 15.5 39.0 80-81 30.80169695551949 35.0 31.0 36.0 10.0 37.5 82-83 30.400125980184317 35.0 31.0 35.0 7.0 37.0 84-85 30.048079594134247 34.0 31.0 35.0 3.5 36.5 86-87 29.687924299798723 34.0 30.5 35.0 2.0 36.0 88-89 29.308856748102986 34.0 30.0 35.0 2.0 36.0 90-91 28.948420617863356 34.0 29.5 35.0 2.0 35.5 92-93 28.5689363812253 34.0 29.0 35.0 2.0 35.0 94-95 28.118037584494445 34.0 29.0 35.0 2.0 35.0 96-97 27.56941459420735 34.0 28.0 35.0 2.0 35.0 98-99 26.904775306909173 34.0 26.0 35.0 2.0 35.0 100-101 24.431342017924763 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14325004873253988 1101 2 0.23107253981710585 1101 3 0.1623713297375602 1101 4 0.0627748020361878 1101 5 0.17003833918526823 1101 6 0.21069633046688807 1101 7 0.05336711830541674 1101 8 0.1730572342604475 1101 9 0.2940274432697194 1101 10-11 0.2436726388181043 1101 12-13 0.3219806868930064 1101 14-15 0.3867621809473647 1101 16-17 0.36022191199917586 1101 18-19 0.2355988141160097 1101 20-21 0.14509357052303073 1101 22-23 0.25284730428438706 1101 24-25 0.3357227291225229 1101 26-27 0.3241378394481771 1101 28-29 0.19980515772479635 1101 30-31 0.16974679233317147 1101 32-33 0.18379670617641608 1101 34-35 0.23922127909523994 1101 36-37 0.13910791598988936 1101 38-39 -0.04983494875862249 1101 40-41 -0.21563886412097588 1101 42-43 -0.1959912747331387 1101 44-45 -0.30006078042453765 1101 46-47 -0.47298485926278744 1101 48-49 0.003410214475216833 1101 50-51 -0.018934614294217056 1101 52-53 0.09680538046848142 1101 54-55 -0.08497992297484558 1101 56-57 -0.12991896158802518 1101 58-59 -0.29722730573353573 1101 60-61 -0.28315485648234784 1101 62-63 -0.32968775334485656 1101 64-65 -0.12803104583152702 1101 66-67 -0.26969798947300916 1101 68-69 -0.11057979664351336 1101 70-71 -0.526021388222091 1101 72-73 -0.7227214085659313 1101 74-75 -0.6155440797567344 1101 76-77 -0.5887454933750362 1101 78-79 -0.9296493461356867 1101 80-81 -1.0253045279243977 1101 82-83 -0.9418942164954984 1101 84-85 -1.0637936454713248 1101 86-87 -1.2544724279162836 1101 88-89 -1.2526923217243464 1101 90-91 -1.310302376343433 1101 92-93 -1.1588385975847508 1101 94-95 -1.555532223859064 1101 96-97 -1.756265277157926 1101 98-99 -1.9927946896654056 1101 100-101 -2.2015700749270515 1102 1 0.06851636258983262 1102 2 0.16326759801505375 1102 3 0.24756835615144723 1102 4 0.27871676599969675 1102 5 0.12873506422218384 1102 6 0.2587313331100347 1102 7 0.3745666961825904 1102 8 0.23603039122810543 1102 9 0.46005201768447535 1102 10-11 0.3128381659742203 1102 12-13 0.277899672081638 1102 14-15 0.38334950126719036 1102 16-17 0.4778759702497908 1102 18-19 0.5075715360864876 1102 20-21 0.475822122528605 1102 22-23 0.4330804861814812 1102 24-25 0.3523579576520959 1102 26-27 0.3535255077657453 1102 28-29 0.27419260171647153 1102 30-31 0.19496102356831102 1102 32-33 0.3441038961141274 1102 34-35 0.2578022909806066 1102 36-37 0.17860780577759527 1102 38-39 0.253300542134717 1102 40-41 0.05464068496445407 1102 42-43 -0.035303706094289566 1102 44-45 -0.1027047049964196 1102 46-47 -0.14712320397172363 1102 48-49 -0.009702122083858455 1102 50-51 0.04548885838777039 1102 52-53 0.04419803077171025 1102 54-55 -0.05960573761922916 1102 56-57 -0.07112801967282678 1102 58-59 -0.0016242884526391776 1102 60-61 -0.03059997953973692 1102 62-63 -0.19809427869271445 1102 64-65 -0.15745480135921497 1102 66-67 -0.29067148585551195 1102 68-69 -0.3533043224868422 1102 70-71 -0.25965486310832375 1102 72-73 -0.4551435235885464 1102 74-75 -0.5787507307270907 1102 76-77 -0.5241952277926778 1102 78-79 -0.7558055510876329 1102 80-81 -0.9437922982614886 1102 82-83 -0.9495369023982754 1102 84-85 -0.9619184760077353 1102 86-87 -1.1890911811714489 1102 88-89 -1.1737900751492205 1102 90-91 -0.8461672112103109 1102 92-93 -0.9069059811839928 1102 94-95 -1.2685384445138226 1102 96-97 -1.3743601845698485 1102 98-99 -1.38433311669219 1102 100-101 -1.2660354867991614 1103 1 0.2405368891832822 1103 2 0.37244701387040635 1103 3 0.45825177661090066 1103 4 0.32970520780104806 1103 5 0.3915637055343524 1103 6 0.29769541157149604 1103 7 0.40493262587420986 1103 8 0.4110646821494015 1103 9 0.38884584052270554 1103 10-11 0.32223819474982207 1103 12-13 0.289031032687852 1103 14-15 0.3958553762851835 1103 16-17 0.5266860992603881 1103 18-19 0.541939192314004 1103 20-21 0.5183179260871853 1103 22-23 0.5613649809299375 1103 24-25 0.6818212927439404 1103 26-27 0.5817371043318573 1103 28-29 0.5742453569800148 1103 30-31 0.4942406744243897 1103 32-33 0.6114128980804665 1103 34-35 0.4802136861175299 1103 36-37 0.29538493876881944 1103 38-39 0.7065910317994835 1103 40-41 0.4625156070305465 1103 42-43 0.5103104131326077 1103 44-45 0.4162123733211729 1103 46-47 0.3898966032317688 1103 48-49 0.19809222830701145 1103 50-51 0.5329839425563918 1103 52-53 0.43246142341475036 1103 54-55 0.3081070819098173 1103 56-57 0.2956553480313673 1103 58-59 0.14563771336981546 1103 60-61 0.5391539890948636 1103 62-63 0.37476937293070733 1103 64-65 0.06188431906666381 1103 66-67 0.21008147563314594 1103 68-69 0.07902314382172904 1103 70-71 0.14368214710701466 1103 72-73 0.17076410674093268 1103 74-75 0.07950259045654207 1103 76-77 0.1165279989110779 1103 78-79 0.171712553559626 1103 80-81 0.1839569703587216 1103 82-83 0.4146710492587182 1103 84-85 0.4879256561681835 1103 86-87 0.41106996217899194 1103 88-89 0.6141510307929394 1103 90-91 0.5206305442930237 1103 92-93 0.5477532677728938 1103 94-95 0.23439474813880778 1103 96-97 0.023982340968490945 1103 98-99 0.11819529921112348 1103 100-101 -0.08046836771614352 1104 1 0.20085113860699977 1104 2 0.2513010384270231 1104 3 0.1867308658520912 1104 4 0.08079331995233474 1104 5 0.08546783123411927 1104 6 0.03669527044151977 1104 7 -0.1497138435241041 1104 8 0.04607997123064678 1104 9 0.09562666566265676 1104 10-11 0.19719703183877613 1104 12-13 -0.013372881316954022 1104 14-15 0.06569025420774466 1104 16-17 0.10098256630654134 1104 18-19 0.16010262083167248 1104 20-21 0.05206869054124752 1104 22-23 0.11318226464824477 1104 24-25 0.2996187241782877 1104 26-27 0.23514162989990695 1104 28-29 0.27317747345928467 1104 30-31 0.21353502452082296 1104 32-33 0.15428724535430405 1104 34-35 0.2551819318718671 1104 36-37 0.2639392973330601 1104 38-39 0.3717403943623978 1104 40-41 0.3897772149714598 1104 42-43 0.555415554968242 1104 44-45 0.39047591003848936 1104 46-47 0.44581700592264895 1104 48-49 0.5462580089910247 1104 50-51 0.3442781044795211 1104 52-53 0.5098354649069634 1104 54-55 0.5702908331447034 1104 56-57 0.505463881690865 1104 58-59 0.4958084806464811 1104 60-61 0.5646712304741683 1104 62-63 0.44573611329859375 1104 64-65 0.5902241600602238 1104 66-67 0.4570871678756987 1104 68-69 0.3524991752466704 1104 70-71 0.33240303904849355 1104 72-73 0.28563920670800513 1104 74-75 0.3169453152060413 1104 76-77 0.22599593888379488 1104 78-79 0.36363709606327177 1104 80-81 0.2098355044886553 1104 82-83 0.20296666952498654 1104 84-85 0.0737609104373611 1104 86-87 0.06162178040357702 1104 88-89 0.0932863744444461 1104 90-91 0.13154930046746216 1104 92-93 0.1005499935573404 1104 94-95 -0.07736702445374988 1104 96-97 0.035184297970889844 1104 98-99 -0.09495989672433325 1104 100-101 -0.1927052779104308 1105 1 0.09211070597369542 1105 2 0.13043908784662506 1105 3 -2.677879133159422E-4 1105 4 -0.025393411977567837 1105 5 -0.06877064512386966 1105 6 -0.056667090136699017 1105 7 0.13383163739883486 1105 8 0.04100500921176575 1105 9 -0.1074799463856877 1105 10-11 0.08429804989490464 1105 12-13 0.16201877923729313 1105 14-15 0.19104145563652253 1105 16-17 -0.18819204951198287 1105 18-19 0.11849820474966322 1105 20-21 0.04137901113250564 1105 22-23 0.046739189466350695 1105 24-25 0.16118994395333885 1105 26-27 0.02860905805116687 1105 28-29 0.1769784686276239 1105 30-31 0.11177176356937935 1105 32-33 0.11895264207900169 1105 34-35 -0.0043214571430709725 1105 36-37 0.16302216374946 1105 38-39 0.3416213237869101 1105 40-41 0.21975607530367824 1105 42-43 0.134152257071932 1105 44-45 0.22538105186168877 1105 46-47 -0.07647245794438362 1105 48-49 0.052546744774012666 1105 50-51 0.045816812779143845 1105 52-53 0.054470260475738996 1105 54-55 0.11487426634929676 1105 56-57 0.116868849602902 1105 58-59 0.08381563893807709 1105 60-61 0.02768311950983815 1105 62-63 0.18728520220233946 1105 64-65 0.22713189343007656 1105 66-67 -0.00825314833623736 1105 68-69 -0.027539968281395488 1105 70-71 -0.026952390313837782 1105 72-73 0.07210333144826109 1105 74-75 -0.03386323653748491 1105 76-77 0.09930210723340238 1105 78-79 -0.08096544033642417 1105 80-81 -0.33998508767435 1105 82-83 -0.5208867332766971 1105 84-85 -0.6360255122370511 1105 86-87 -0.5190252412100662 1105 88-89 -0.4944026976268603 1105 90-91 -0.33773961012778386 1105 92-93 -0.43996139537109613 1105 94-95 -0.5239744952447865 1105 96-97 -0.34874667101164647 1105 98-99 -0.558120466287896 1105 100-101 -0.5653806803841164 1106 1 0.0941727187128123 1106 2 0.20495029041038038 1106 3 0.19007624445561078 1106 4 0.26660040371554317 1106 5 0.21004429485961396 1106 6 0.298695095215912 1106 7 0.17768446751564682 1106 8 0.21671931223934138 1106 9 0.14256030701161393 1106 10-11 0.34207820921178467 1106 12-13 0.3563379427976656 1106 14-15 0.1906752795707547 1106 16-17 0.30379189760630254 1106 18-19 0.33770002749274397 1106 20-21 0.3276386654553747 1106 22-23 0.3459846763247043 1106 24-25 0.38466035813915767 1106 26-27 0.2873284633050588 1106 28-29 0.30623342585514024 1106 30-31 0.31947409965148665 1106 32-33 0.5044702488284187 1106 34-35 0.4063220605017719 1106 36-37 0.4602134288488102 1106 38-39 0.6628607475313899 1106 40-41 0.633541954931303 1106 42-43 0.5464111045955278 1106 44-45 0.5690853339574957 1106 46-47 0.8454350856288428 1106 48-49 0.7966848978962062 1106 50-51 0.7888469153950055 1106 52-53 0.8702360878518647 1106 54-55 0.9438977449007311 1106 56-57 1.028782297007723 1106 58-59 0.8600309885539801 1106 60-61 1.0011063549826886 1106 62-63 0.9251801832529409 1106 64-65 0.6586041636004936 1106 66-67 0.5793633484582017 1106 68-69 0.6932763186759772 1106 70-71 0.47504383192647026 1106 72-73 0.5879874085893491 1106 74-75 0.46412409359750484 1106 76-77 0.5767376974694081 1106 78-79 0.5578308705479209 1106 80-81 0.723573728165924 1106 82-83 0.9207631539272256 1106 84-85 0.756742034348683 1106 86-87 0.7668154315150986 1106 88-89 0.9062276792314989 1106 90-91 0.7577752091820749 1106 92-93 0.5853679466719761 1106 94-95 0.7226299359184445 1106 96-97 0.7576028046553915 1106 98-99 0.7968937219525714 1106 100-101 0.6047795194804912 1107 1 0.11077329160062988 1107 2 0.11523051902504733 1107 3 0.07112770977833094 1107 4 0.12109162799301032 1107 5 0.1638281918478981 1107 6 0.2920626351761868 1107 7 0.26715089818135596 1107 8 0.24124776071321463 1107 9 0.4298554388163396 1107 10-11 0.41559937498444555 1107 12-13 0.4466074635445878 1107 14-15 0.42991421027024757 1107 16-17 0.46361020164219013 1107 18-19 0.33902415498731386 1107 20-21 0.32658223465454483 1107 22-23 0.3909534985268053 1107 24-25 0.20439239121532182 1107 26-27 0.13678242960736497 1107 28-29 0.09908094176493876 1107 30-31 0.42664735768070017 1107 32-33 0.2678677505511331 1107 34-35 0.3687668322248925 1107 36-37 0.5030955679902931 1107 38-39 0.4266745725033516 1107 40-41 0.34554084698788756 1107 42-43 0.3582752193892773 1107 44-45 0.35385634772571706 1107 46-47 0.2436292729793692 1107 48-49 0.16901384224231464 1107 50-51 0.36330121948494565 1107 52-53 0.2454404493656881 1107 54-55 0.20440462901255074 1107 56-57 0.14049626520164082 1107 58-59 0.07732807952694287 1107 60-61 -0.08674629529902944 1107 62-63 -0.033341185192284684 1107 64-65 -0.0032045540225595914 1107 66-67 0.2092460685943962 1107 68-69 -0.04608083769266358 1107 70-71 -0.07507540710557237 1107 72-73 -0.16720692063197617 1107 74-75 -0.0675139399380491 1107 76-77 -0.09767303201675404 1107 78-79 -0.07374247665722322 1107 80-81 -0.1904120307077548 1107 82-83 -0.26689222838080795 1107 84-85 -0.26726453224276625 1107 86-87 -0.21085680639548343 1107 88-89 -0.2421607360040703 1107 90-91 -0.2674693490592652 1107 92-93 -0.32222095903123105 1107 94-95 -0.5498498306027493 1107 96-97 -0.37747340023307174 1107 98-99 -0.32323200260188756 1107 100-101 -0.24819588282456806 1108 1 0.0905601027906755 1108 2 0.24001127335633043 1108 3 0.16047268574017437 1108 4 2.0366726164411375E-4 1108 5 0.038154681772056165 1108 6 0.10907767637544907 1108 7 0.11375464081533693 1108 8 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2316 52-53 -1.6810431709929219 2316 54-55 -1.528069843953908 2316 56-57 -1.5508980228476688 2316 58-59 -1.5646768141801601 2316 60-61 -1.4506105269279388 2316 62-63 -1.592876775341857 2316 64-65 -1.5708831940249226 2316 66-67 -1.5084278943237024 2316 68-69 -1.4043276449672533 2316 70-71 -1.2836056703023324 2316 72-73 -1.3337117355671424 2316 74-75 -1.5436281937215668 2316 76-77 -1.4826506066285567 2316 78-79 -1.1019046779392383 2316 80-81 -1.0071835625266985 2316 82-83 -1.1566921230233334 2316 84-85 -1.1308241379891832 2316 86-87 -0.9805079451424206 2316 88-89 -1.1886905856481214 2316 90-91 -1.1563381346912642 2316 92-93 -1.4443193205747669 2316 94-95 -1.2947222692837101 2316 96-97 -1.3893470401862054 2316 98-99 -1.2765239314314343 2316 100-101 -0.8287765059106569 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2715.0 3 617.0 4 652.0 5 715.0 6 787.0 7 954.0 8 1188.0 9 1449.0 10 1752.0 11 2239.0 12 3075.0 13 4634.0 14 4053.0 15 4125.0 16 5192.0 17 7446.0 18 6977.0 19 4298.0 20 4303.0 21 4540.0 22 4894.0 23 5220.0 24 6068.0 25 6720.0 26 7651.0 27 9187.0 28 10747.0 29 12892.0 30 15465.0 31 19216.0 32 24042.0 33 31941.0 34 45066.0 35 67588.0 36 110671.0 37 179064.0 38 172355.0 39 30254.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46428877039341 18.368674162687615 14.254541987252365 22.912495079666613 2 15.569363329869192 20.300599637972844 40.147689515361755 23.982347516796207 3 20.052495615201284 24.53682133195427 29.138603548332803 26.272079504511648 4 13.190382731755989 16.08590697680092 36.27260717940711 34.45110311203598 5 14.270202873584953 37.25571050894686 33.038174979534524 15.435911637933664 6 32.42572899175803 34.69553245999432 17.499338471912797 15.379400076334854 7 29.080711801405844 32.69032833986176 20.134413978468398 18.094545880263997 8 26.616502841532718 34.11753945218176 19.62608844118149 19.639869265104025 9 27.257617458298633 17.15429537215096 20.159818158981633 35.42826901056878 10-11 25.71006306794071 26.186060180375286 26.354399879034425 21.749476872649584 12-13 26.904402892296176 23.540422698635005 27.125977314535078 22.429197094533745 14-15 23.56609991738082 24.695025471706742 26.118026529598094 25.620848081314346 16-17 22.59750774751662 28.11914135018653 26.549884374068334 22.73346652822852 18-19 22.553646603154164 26.577751598788286 28.910689808242967 21.957911989814587 20-21 23.585686913739252 25.6427753405511 28.620623206382078 22.15091453932757 22-23 23.047354076419563 25.679830165193746 28.539602405545857 22.733213352840835 24-25 23.00755842039261 25.82415571614691 28.71949672338328 22.448789140077192 26-27 22.799705765388154 25.839032197464118 28.93166730506957 22.429594732078158 28-29 22.431394266601448 26.276084328604902 28.967495453120485 22.325025951673165 30-31 22.58841193628986 26.03664363657875 28.69029766322567 22.684646763905718 32-33 22.39088119502593 26.036976237053803 28.732199743393355 22.83994282452691 34-35 23.057201754487824 25.981307383113084 28.44228396325525 22.51920689914384 36-37 23.15895856275637 25.775651357378397 28.559334535974244 22.506055543890984 38-39 22.853032091684582 25.98380575225653 28.771441527945818 22.39172062811307 40-41 22.862687920528682 25.957766790402108 28.70966182717705 22.469883461892152 42-43 22.69324882273952 26.07703879971454 28.26851209209011 22.96120028545583 44-45 22.758684009181334 26.18680948737567 28.12266258607498 22.93184391736802 46-47 22.929645057524738 26.34207554272807 27.719547392487353 23.008732007259837 48-49 23.174315341469118 26.370939402807082 27.578695784729423 22.876049470994378 50-51 22.89840878276013 26.63506064621967 27.627069362870373 22.83946120814982 52-53 23.154101327084483 26.689157133928465 27.29304515662217 22.86369638236488 54-55 22.685775141844623 26.44051094667132 27.551091606837918 23.32262230464614 56-57 22.932740321538372 26.81195782011867 27.185119548828013 23.07018230951495 58-59 23.21430210254857 26.343437680680598 27.313920015662806 23.12834020110802 60-61 23.011604972248982 26.37304099200343 27.811323639588398 22.8040303961592 62-63 22.990029400172826 26.298099637454985 27.774044616781847 22.93782634559034 64-65 22.64936047618552 26.408510414262548 27.702556687221954 23.23957242232998 66-67 22.59809005562814 26.56583479556689 27.397256919232056 23.438818229572913 68-69 22.556742719596944 26.48561003283904 27.607819892879686 23.349827354684326 70-71 22.743717897616474 26.460476681171478 27.408095877486073 23.38770954372598 72-73 22.727601098563373 26.300442143594427 27.392577228196785 23.579379529645415 74-75 22.580485182595535 26.80400567691311 27.131566992105466 23.48394214838589 76-77 22.72750365887474 26.42010553963735 27.201216380740885 23.65117442074703 78-79 22.635461182145484 26.495245800411134 27.341620007968036 23.527673009475354 80-81 22.642076402403948 26.710424852201374 27.272794036646715 23.374704708747966 82-83 22.64799980903053 26.482874873987694 27.363843970598044 23.50528134638373 84-85 22.547792454689517 26.365802557622402 27.17900724882068 23.9073977388674 86-87 22.579170204079386 26.3171876329399 27.400232719420075 23.703409443560645 88-89 22.61123647231531 26.23155649641078 27.141136037788748 24.01607099348516 90-91 22.48617948998966 26.147811035713215 27.400703255633974 23.965306218663155 92-93 22.55400145220163 26.043754614658422 27.46044709688242 23.94179683625753 94-95 22.420449786568117 25.903687485765087 27.42807847631922 24.24778425134757 96-97 22.350911371836403 25.722047355457363 27.509402702615 24.41763857009123 98-99 22.25846541897013 25.847031669942023 27.21766121182067 24.676841699267175 100-101 22.514584085747693 25.237048780935705 27.752183787026436 24.496183346290163 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 876.0 1 508.5 2 167.5 3 202.0 4 180.0 5 172.5 6 310.5 7 648.5 8 1013.5 9 1169.5 10 1067.0 11 1004.5 12 1093.5 13 1137.5 14 1189.5 15 1285.5 16 1397.5 17 1440.5 18 1468.5 19 1474.5 20 1565.5 21 1771.5 22 2052.0 23 2409.5 24 2700.0 25 3193.0 26 3986.5 27 4924.5 28 5737.0 29 6739.5 30 7993.0 31 9243.5 32 10645.0 33 11792.5 34 12868.5 35 14012.0 36 15453.0 37 17291.5 38 18679.5 39 19348.0 40 19864.5 41 21398.0 42 23140.0 43 24538.5 44 26049.5 45 27404.5 46 28619.0 47 31088.0 48 37824.5 49 38739.0 50 33801.0 51 31966.5 52 30891.0 53 30545.0 54 30142.5 55 28716.5 56 26182.5 57 23349.0 58 21321.0 59 19083.0 60 16241.0 61 13579.0 62 11237.5 63 8927.5 64 6657.5 65 4957.0 66 3623.0 67 2882.0 68 2173.0 69 1518.0 70 1154.0 71 841.0 72 651.0 73 494.5 74 392.5 75 324.0 76 229.5 77 129.5 78 89.5 79 60.5 80 37.0 81 29.0 82 19.5 83 11.0 84 8.5 85 9.5 86 8.5 87 8.5 88 7.0 89 5.0 90 4.0 91 2.5 92 2.0 93 1.0 94 3.0 95 3.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33261692764302525 2 0.11404008947760867 3 0.10794820435594153 4 0.11538030420437545 5 0.1329249333547768 6 0.08443352778630642 7 0.09259665384934036 8 0.09552075870774059 9 0.08613925562037321 10-11 0.08479904089360644 12-13 0.08814957771052336 14-15 0.08973346784215683 16-17 0.08071747786208947 18-19 0.09357135546880711 20-21 0.1027091831513078 22-23 0.11068955266069175 24-25 0.12378710567227608 26-27 0.12305607945767602 28-29 0.11830440906277567 30-31 0.10898382482662494 32-33 0.10136896842454104 34-35 0.3208595893582077 36-37 0.48131984346291995 38-39 0.45634311446408465 40-41 0.43416865262121634 42-43 0.4660292118075354 44-45 0.4738268247632693 46-47 0.4802233041410199 48-49 0.4925898309380041 50-51 0.4796141156288531 52-53 0.4841221106188868 54-55 0.46895331666593565 56-57 0.4498247973839009 58-59 0.43148822316768276 60-61 0.39310934690117993 62-63 0.4583534365542348 64-65 0.4902139957405539 66-67 0.4984380406548045 68-69 0.5123884575834223 70-71 0.5042253315203883 72-73 0.49478290958180426 74-75 0.5023368471326715 76-77 0.47693368617531956 78-79 0.4568304652738181 80-81 0.45981548898343494 82-83 0.47266936659015263 84-85 0.4718165026731192 86-87 0.4752279583412528 88-89 0.4889346998650039 90-91 0.5036161430082217 92-93 0.4962449620110044 94-95 0.5007529570010381 96-97 0.5008138758522548 98-99 0.49453923417693757 100-101 0.48113708690926993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 820764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.88906885965404 #Duplication Level Percentage of deduplicated Percentage of total 1 81.11772347271527 51.82535820686681 2 11.482399704503846 14.67199650790235 3 3.3588972355190636 6.437904503177371 4 1.416143324080732 3.6190431338933307 5 0.7037013232746768 2.2479411149662747 6 0.4347266271856614 1.6664567651633926 7 0.2766750191371308 1.2373556544578774 8 0.19450303383872297 0.9941294177867035 9 0.13918831463374617 0.8003350636285143 >10 0.7946808735309514 9.258851631493178 >50 0.05271414712416637 2.277911040263754 >100 0.02635517051992364 3.076242722875992 >500 0.0011458769680480067 0.5028239009661939 >1k 0.0011458769680480067 1.383650336558272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3349 0.40803446544926436 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2492 0.3036195544638898 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1723 0.2099263612926493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 0.20103220901501526 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1116 0.13597087591561033 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1010 0.12305607945767602 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 864 0.105267774902408 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.0918851216671295E-5 0.0 20-21 0.0 0.0 0.0 2.4367540486668518E-4 0.0 22-23 0.0 0.0 0.0 3.045942560833565E-4 0.0 24-25 0.0 0.0 0.0 4.873508097333704E-4 0.0 26-27 0.0 0.0 0.0 0.0011574581731167545 0.0 28-29 0.0 0.0 0.0 0.0035942122217836065 0.0 30-31 0.0 0.0 0.0 0.010660798962917478 0.0 32-33 1.2183770243334259E-4 0.0 0.0 0.026804294535335373 0.0 34-35 1.2183770243334259E-4 0.0 0.0 0.05019713340253715 0.0 36-37 1.2183770243334259E-4 0.0 0.0 0.08839325311539005 0.0 38-39 1.2183770243334259E-4 0.0 0.0 0.14992129284422806 0.0 40-41 1.2183770243334259E-4 0.0 0.0 0.2181504062068999 6.0918851216671295E-5 42-43 1.2183770243334259E-4 0.0 0.0 0.28308990160387154 1.2183770243334259E-4 44-45 1.2183770243334259E-4 0.0 0.0 0.3656349450024611 1.2183770243334259E-4 46-47 1.2183770243334259E-4 0.0 0.0 0.4651154290392854 1.2183770243334259E-4 48-49 1.2183770243334259E-4 0.0 0.0 0.5738555784610436 2.4367540486668518E-4 50-51 1.2183770243334259E-4 0.0 0.0 0.6943530661676194 2.4367540486668518E-4 52-53 1.2183770243334259E-4 0.0 0.0 0.8177746587325955 2.4367540486668518E-4 54-55 1.2183770243334259E-4 0.0 0.0 0.955755856738356 2.4367540486668518E-4 56-57 1.2183770243334259E-4 0.0 0.0 1.1128655740261513 2.4367540486668518E-4 58-59 1.2183770243334259E-4 0.0 0.0 1.2879463524228645 2.4367540486668518E-4 60-61 1.2183770243334259E-4 0.0 0.0 1.488003859818413 2.4367540486668518E-4 62-63 1.2183770243334259E-4 0.0 0.0 1.7005497317133793 2.4367540486668518E-4 64-65 1.2183770243334259E-4 0.0 0.0 1.919857596093396 2.4367540486668518E-4 66-67 1.2183770243334259E-4 0.0 0.0 2.1699294803378315 2.4367540486668518E-4 68-69 1.2183770243334259E-4 0.0 0.0 2.4609997514510873 2.4367540486668518E-4 70-71 1.2183770243334259E-4 0.0 0.0 2.7737571335974773 2.4367540486668518E-4 72-73 1.2183770243334259E-4 0.0 0.0 3.100952283482219 2.4367540486668518E-4 74-75 1.2183770243334259E-4 0.0 0.0 3.4492862747391455 2.4367540486668518E-4 76-77 1.2183770243334259E-4 0.0 0.0 3.82070851060719 2.4367540486668518E-4 78-79 1.2183770243334259E-4 0.0 0.0 4.246653118314156 2.4367540486668518E-4 80-81 1.2183770243334259E-4 0.0 0.0 4.709027199048691 2.4367540486668518E-4 82-83 1.2183770243334259E-4 0.0 0.0 5.187727531909294 2.4367540486668518E-4 84-85 1.2183770243334259E-4 0.0 0.0 5.673189857254949 2.4367540486668518E-4 86-87 1.2183770243334259E-4 0.0 0.0 6.193863278603837 2.4367540486668518E-4 88-89 1.2183770243334259E-4 0.0 0.0 6.761139621133481 3.045942560833565E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAT 105 0.0022075148 22.598143 1 GTATCAA 6760 0.0 22.18362 1 TACATGG 4680 0.0 22.067863 2 GTACATG 4735 0.0 21.949072 1 GAGTACT 2930 0.0 21.818775 12-13 ACATGGG 4745 0.0 21.366196 3 AGTACTT 2960 0.0 21.117693 12-13 GTACTTT 3065 0.0 20.318235 14-15 TAGTACA 210 8.821753E-7 20.30359 4 GGTATCA 5145 0.0 20.015497 1 AGTACAT 3670 0.0 19.621271 2 GTCCTAC 220 1.3557383E-6 19.413857 1 TATCAAC 7700 0.0 19.319174 2 CATGGGG 2065 0.0 19.271206 4 TCAACGC 7810 0.0 18.986414 4 GTACACG 175 1.0003048E-4 18.982439 1 GTATAGA 275 2.8645445E-8 18.982439 1 ATCAACG 7830 0.0 18.877413 3 CAACGCA 7890 0.0 18.793903 5 GTATATA 330 6.039045E-10 18.694826 1 >>END_MODULE