FastQCFastQC Report
Fri 27 May 2016
SRR939147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820764
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.4243607175753322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.32262623604349117No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19060.23222266083795096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.20578387940991563No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12020.1464489183248778No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9550.11635500582384219No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8690.10587696341457471No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG650.005871482729.2018075
GTACATG47150.026.2502061
TACATGG46700.025.9111352
ACATGGG47600.024.3246173
GTAAGGT1903.3160904E-722.4777094
GTATAGG2154.3168257E-822.1412731
CATGGGG19750.022.104664
GAGTACT31000.021.50689712-13
TATAGGG2005.4337943E-721.353822
GGTATCA59100.021.1840671
TAAGGTG3151.4551915E-1121.0901975
GTGTAGC4550.020.924721
GTATCAA73850.020.5627541
GTACTTT32550.020.19118914-15
CATGGGA28200.020.0244674
AGTACTT31950.019.90201612-13
ATGGGGG9500.018.4816725
GTAGCCC3156.881237E-918.077313
TATCAAC86000.017.8776192
CAACGCA85450.017.7150235
Produced by FastQC (version 0.11.5)