##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939147_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 820764 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.494541670930985 34.0 31.0 34.0 31.0 34.0 2 32.695313147262794 34.0 31.0 34.0 31.0 34.0 3 32.78767465434644 34.0 31.0 34.0 31.0 34.0 4 36.24571740475947 37.0 35.0 37.0 35.0 37.0 5 36.20729954042819 37.0 37.0 37.0 35.0 37.0 6 36.248285743526765 37.0 37.0 37.0 35.0 37.0 7 36.20003679498613 37.0 37.0 37.0 35.0 37.0 8 36.191002529350705 37.0 37.0 37.0 35.0 37.0 9 37.928834598008685 39.0 38.0 39.0 35.0 39.0 10-11 37.869297995526125 39.0 38.0 39.0 35.0 39.0 12-13 37.80278923052181 39.0 38.0 39.0 35.0 39.0 14-15 39.1515673202041 40.5 38.5 41.0 36.0 41.0 16-17 39.09693468524448 40.0 38.0 41.0 36.0 41.0 18-19 39.13684445224206 40.5 38.5 41.0 36.0 41.0 20-21 39.20717892110278 41.0 39.0 41.0 36.0 41.0 22-23 39.16503038632298 41.0 39.0 41.0 36.0 41.0 24-25 39.106607989629175 41.0 39.0 41.0 35.0 41.0 26-27 38.971528356506866 40.0 38.5 41.0 35.0 41.0 28-29 38.81714719456507 40.0 38.0 41.0 35.0 41.0 30-31 38.63146470849111 40.0 38.0 41.0 34.5 41.0 32-33 38.48859781861778 40.0 38.0 41.0 34.0 41.0 34-35 38.43664013041507 40.0 38.0 41.0 34.0 41.0 36-37 38.33263703086393 40.0 38.0 41.0 34.0 41.0 38-39 38.20490786632942 40.0 38.0 41.0 34.0 41.0 40-41 38.03767343596941 40.0 38.0 41.0 33.0 41.0 42-43 37.85759487501888 40.0 37.5 41.0 33.0 41.0 44-45 37.619572130356595 40.0 37.0 41.0 33.0 41.0 46-47 37.293793343762644 40.0 37.0 41.0 32.5 41.0 48-49 36.95000450799499 40.0 36.5 41.0 31.0 41.0 50-51 36.68060916414463 40.0 36.0 41.0 31.0 41.0 52-53 36.4385237412947 39.0 36.0 41.0 30.5 41.0 54-55 36.19192788670068 39.0 35.0 41.0 30.0 41.0 56-57 35.91865871310145 39.0 35.0 40.5 29.5 41.0 58-59 35.62037504081563 38.5 35.0 40.0 29.0 41.0 60-61 35.25476507254217 38.0 35.0 40.0 28.0 41.0 62-63 34.861209190461565 37.5 34.0 40.0 28.0 41.0 64-65 34.44545569737464 37.0 34.0 39.5 27.0 41.0 66-67 34.08454074496444 36.0 34.0 39.0 26.0 41.0 68-69 33.62615258466502 36.0 33.5 39.0 26.0 40.0 70-71 33.19342405368657 35.0 33.0 38.5 25.5 40.0 72-73 32.742072629891176 35.0 33.0 37.5 24.5 39.5 74-75 32.26429704519204 35.0 32.0 37.0 23.5 39.0 76-77 30.29505558235985 33.5 29.5 35.0 20.0 37.5 78-79 31.274142140737165 34.5 31.0 36.0 21.0 38.0 80-81 31.326740086066152 35.0 32.0 35.5 21.5 37.0 82-83 31.060090354840124 35.0 31.5 35.0 20.0 37.0 84-85 30.714527562125042 34.0 31.0 35.0 19.0 36.5 86-87 30.201267964969222 34.0 31.0 35.0 14.5 36.0 88-89 29.686667543898125 34.0 30.0 35.0 7.0 35.5 90-91 29.25892948521134 34.0 29.5 35.0 3.5 35.0 92-93 29.020883591385587 34.0 29.0 35.0 2.0 35.0 94-95 28.72296908733814 34.0 29.0 35.0 2.0 35.0 96-97 28.1966454425389 34.0 29.0 35.0 2.0 35.0 98-99 27.476218498862035 34.0 28.5 35.0 2.0 35.0 100-101 23.93374526660526 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20880779994440957 1101 2 0.08052857025525384 1101 3 0.07163856092211773 1101 4 0.10648555305411378 1101 5 0.13548502804710694 1101 6 0.07994702416936406 1101 7 0.12504458269352625 1101 8 0.1728624130979881 1101 9 0.08376131249214325 1101 10-11 0.1506718880070963 1101 12-13 0.10490852197614942 1101 14-15 0.09801927908233665 1101 16-17 0.12010941907944073 1101 18-19 0.1743023532717629 1101 20-21 0.31494947092217274 1101 22-23 0.1656867298738689 1101 24-25 0.1666294031609823 1101 26-27 0.28578446682340797 1101 28-29 0.15146753473266727 1101 30-31 0.1614344540781758 1101 32-33 -0.137677285098178 1101 34-35 0.05784665659096788 1101 36-37 -0.02720253125558969 1101 38-39 -0.05667951995632592 1101 40-41 -0.0027137619178390082 1101 42-43 -0.07603149063845649 1101 44-45 -0.0922771497053887 1101 46-47 -0.12880448396850852 1101 48-49 -0.2403380630406744 1101 50-51 -0.18514759589653096 1101 52-53 -0.23780658879078231 1101 54-55 -0.20391771671025793 1101 56-57 -0.24865362624679932 1101 58-59 -0.2832131127486477 1101 60-61 -0.3010157565950067 1101 62-63 -0.4561333588150376 1101 64-65 -0.493110850618514 1101 66-67 -0.6241095260094838 1101 68-69 -0.6289260127933858 1101 70-71 -0.5270772886961055 1101 72-73 -0.5487752968840027 1101 74-75 -0.2121969453014927 1101 76-77 -0.29586799179807954 1101 78-79 -0.2098853997964909 1101 80-81 -0.41643622209761944 1101 82-83 -0.4496532810373104 1101 84-85 -0.31527680153007864 1101 86-87 -0.41247102510207867 1101 88-89 -0.1397150270776919 1101 90-91 -0.3175197402435401 1101 92-93 -0.3004656782709958 1101 94-95 -0.5054101621395262 1101 96-97 -0.3959210914280753 1101 98-99 -0.58682902032767 1101 100-101 -1.1254662531782849 1102 1 0.1554338408653564 1102 2 0.05167440744926921 1102 3 0.05600207031551463 1102 4 0.043672350206925614 1102 5 0.11585748706861665 1102 6 0.14383894199976055 1102 7 0.0553943165259696 1102 8 0.02775671658557144 1102 9 0.036110589870148146 1102 10-11 0.12668267125560817 1102 12-13 0.1374416295530949 1102 14-15 0.24098997821838708 1102 16-17 0.23910943273406104 1102 18-19 0.06652741262059436 1102 20-21 0.1525970139683679 1102 22-23 0.1328990611661638 1102 24-25 0.18223468320748282 1102 26-27 0.1791906508057579 1102 28-29 0.1633714374181423 1102 30-31 0.001001446570086273 1102 32-33 0.142229746445949 1102 34-35 0.15506950194068736 1102 36-37 0.17519307429754605 1102 38-39 0.194822975913155 1102 40-41 0.18373714874778102 1102 42-43 0.08132044939045358 1102 44-45 0.20269287840362438 1102 46-47 8.841711879625791E-4 1102 48-49 -0.025435800860272195 1102 50-51 0.11926070065060657 1102 52-53 0.11431387616853073 1102 54-55 0.20203803841948087 1102 56-57 0.14977940857053085 1102 58-59 0.24889475094703783 1102 60-61 0.30091208068873954 1102 62-63 0.29004285990856715 1102 64-65 0.19560282565385734 1102 66-67 0.17028895871782623 1102 68-69 0.25419824229832244 1102 70-71 0.06607062790148177 1102 72-73 -0.15481317479999746 1102 74-75 -0.07933359098556281 1102 76-77 0.00629607041237179 1102 78-79 0.21079003573712285 1102 80-81 0.29256724422521074 1102 82-83 0.1450317556448475 1102 84-85 0.20593077405312243 1102 86-87 4.2322766834601566E-4 1102 88-89 -0.21643350977631215 1102 90-91 -0.1243712384479565 1102 92-93 -0.16242722295964285 1102 94-95 -0.19357765361026225 1102 96-97 -0.2008190036366706 1102 98-99 0.2971662044566372 1102 100-101 -0.2623382138061636 1103 1 0.2657019335227844 1103 2 0.2536426583724918 1103 3 0.1482105974844785 1103 4 0.13224364175169967 1103 5 0.25103757435459784 1103 6 0.18460437291435028 1103 7 0.19655205476394144 1103 8 0.20919070056715583 1103 9 0.1933864998984447 1103 10-11 0.23481465533311052 1103 12-13 0.2211603960391173 1103 14-15 0.3346977140583931 1103 16-17 0.4078639289212944 1103 18-19 0.27019150546986026 1103 20-21 0.39224211492275174 1103 22-23 0.3654816711129243 1103 24-25 0.3113747724118383 1103 26-27 0.28677493932909215 1103 28-29 0.3192162410246837 1103 30-31 0.2625342267724733 1103 32-33 0.3561245236748647 1103 34-35 0.38999348640187037 1103 36-37 0.4714800629311853 1103 38-39 0.3787143959889292 1103 40-41 0.31261921675411486 1103 42-43 0.3974472538602569 1103 44-45 0.515539264668071 1103 46-47 0.5484262555004662 1103 48-49 0.4969439411185306 1103 50-51 0.4655270667721325 1103 52-53 0.5357785541216273 1103 54-55 0.6585159051884659 1103 56-57 0.5719625172943381 1103 58-59 0.6508160017263762 1103 60-61 0.537014905493308 1103 62-63 0.5397306446671024 1103 64-65 0.44188262038792203 1103 66-67 0.5156767099048238 1103 68-69 0.6082225097195533 1103 70-71 0.5866423031208896 1103 72-73 0.4967661259529592 1103 74-75 0.2637444388451442 1103 76-77 0.34621741711037046 1103 78-79 0.1734680847515584 1103 80-81 0.33880099800200725 1103 82-83 0.3602185572371006 1103 84-85 0.48609767252321845 1103 86-87 0.576138541082571 1103 88-89 0.7239798996524875 1103 90-91 0.8179941157316968 1103 92-93 0.5933264579189697 1103 94-95 0.32136935132954747 1103 96-97 0.2896744978740493 1103 98-99 0.6037711634514089 1103 100-101 0.3754690856039389 1104 1 0.07840363178688392 1104 2 0.09714456362722501 1104 3 0.10100355980277698 1104 4 0.061179992130398375 1104 5 0.02492476445668501 1104 6 0.08967093922191793 1104 7 -0.007190712324543824 1104 8 0.02583651135889653 1104 9 0.1057673626730562 1104 10-11 -0.04554569655097396 1104 12-13 0.0016402379735396266 1104 14-15 0.09436276557084256 1104 16-17 0.20224225135625318 1104 18-19 0.1713911103059118 1104 20-21 0.06833093272395274 1104 22-23 0.08940508457672536 1104 24-25 0.10123693546400858 1104 26-27 0.014338565428730021 1104 28-29 -0.049492419651798514 1104 30-31 -0.04411958138483385 1104 32-33 0.0117231500097148 1104 34-35 0.022618033609845156 1104 36-37 0.10500501941611162 1104 38-39 0.21221677167693542 1104 40-41 0.28995656445133733 1104 42-43 0.3493256939035376 1104 44-45 0.5149690995459935 1104 46-47 0.5865031242966268 1104 48-49 0.5434942539566592 1104 50-51 0.4834270526591382 1104 52-53 0.5351566413399524 1104 54-55 0.6720813189345236 1104 56-57 0.9288059100590758 1104 58-59 0.8567848590439624 1104 60-61 0.8994668036915456 1104 62-63 0.8715187615227222 1104 64-65 0.590268563841839 1104 66-67 0.7491169884013118 1104 68-69 0.6478649804350809 1104 70-71 0.60263327080213 1104 72-73 0.669318226437511 1104 74-75 0.6335454455723308 1104 76-77 0.5509076368027088 1104 78-79 0.7337964471733507 1104 80-81 0.7764855254517933 1104 82-83 0.8360608680053154 1104 84-85 0.7431508785259489 1104 86-87 0.9243683006911674 1104 88-89 1.017581066326997 1104 90-91 0.8224246562595212 1104 92-93 1.0203603761576687 1104 94-95 0.8506952204550124 1104 96-97 0.765190327953551 1104 98-99 0.9538374945423449 1104 100-101 0.43110064226762645 1105 1 -0.046264540307674906 1105 2 -0.011684176228705212 1105 3 -0.08747108611229493 1105 4 -0.08355374357149259 1105 5 -0.0012249865710103336 1105 6 0.04015942233811387 1105 7 -0.04568545263823154 1105 8 -0.028054322892053563 1105 9 -0.07068128127041717 1105 10-11 -0.11923627164726014 1105 12-13 -0.09077236941435984 1105 14-15 -0.04794702697927988 1105 16-17 -0.039801538213041 1105 18-19 -0.1448451637780792 1105 20-21 -0.12322365285385928 1105 22-23 -0.09483081465211995 1105 24-25 -0.22709832921436401 1105 26-27 -0.07687558679490536 1105 28-29 -0.03620488592834903 1105 30-31 -0.17552253084607372 1105 32-33 -0.1376467148802334 1105 34-35 0.07540322538970656 1105 36-37 0.1273521533060844 1105 38-39 0.10107762652690155 1105 40-41 0.07887166516663058 1105 42-43 -0.006902037808309558 1105 44-45 0.14477080477647775 1105 46-47 0.12853375789193677 1105 48-49 0.26971212769895203 1105 50-51 0.3816205391904859 1105 52-53 0.43297347053395896 1105 54-55 0.5089436371613161 1105 56-57 0.29820403326026224 1105 58-59 0.2471181016671551 1105 60-61 0.39924603563497385 1105 62-63 0.3783076045359053 1105 64-65 0.1923021530258282 1105 66-67 -0.018739542999114178 1105 68-69 0.06427498546318589 1105 70-71 0.15055976314913266 1105 72-73 0.014497643285452 1105 74-75 -0.1335388481771318 1105 76-77 0.11920516365981726 1105 78-79 0.037910302216140224 1105 80-81 0.16084992070049964 1105 82-83 0.23032378203552994 1105 84-85 0.20364060515202098 1105 86-87 0.3885687328559051 1105 88-89 0.29774293887264136 1105 90-91 0.38403155936126865 1105 92-93 0.19314607255022764 1105 94-95 -0.07759571039322566 1105 96-97 0.036322336438253444 1105 98-99 0.11552081042448847 1105 100-101 -0.20166205820328642 1106 1 0.008983896735145436 1106 2 -0.03421742207221712 1106 3 -0.06556804399426142 1106 4 0.042235958830382 1106 5 -0.04747916989450829 1106 6 0.01024725265403248 1106 7 0.013221282404380474 1106 8 -0.00987017528587586 1106 9 0.01800257980082165 1106 10-11 -0.03355972890334158 1106 12-13 -0.09658444058582205 1106 14-15 -0.016922478466575797 1106 16-17 -0.17738938151391892 1106 18-19 -0.18688356119077554 1106 20-21 -0.10173266701785622 1106 22-23 -0.07649690709469326 1106 24-25 -0.06840558642578287 1106 26-27 -0.013264831087141715 1106 28-29 0.09772033105044642 1106 30-31 -0.16022710553647102 1106 32-33 -0.07178436591703985 1106 34-35 -0.33049731668937454 1106 36-37 -0.1746201765951838 1106 38-39 -0.034723485332534665 1106 40-41 -0.01867919694860376 1106 42-43 0.08840761902499139 1106 44-45 0.023094739879347514 1106 46-47 0.2408563183196435 1106 48-49 0.2264020691973414 1106 50-51 0.18236622255466983 1106 52-53 0.17432331843671278 1106 54-55 0.08182046522824038 1106 56-57 0.014707579887549116 1106 58-59 -0.0485521909793718 1106 60-61 -0.04428667176524925 1106 62-63 0.1292827755479209 1106 64-65 0.1504576644517357 1106 66-67 0.06388206987227818 1106 68-69 -0.171233358676524 1106 70-71 -0.12282486368889067 1106 72-73 -0.004009397163585504 1106 74-75 0.16339012059142277 1106 76-77 -0.030512370027512503 1106 78-79 0.21678168352228866 1106 80-81 0.08982734590276209 1106 82-83 0.1726549471679526 1106 84-85 0.24575691236594466 1106 86-87 0.34671385630831253 1106 88-89 0.14206097706922094 1106 90-91 0.36458786996860226 1106 92-93 0.5303518115593207 1106 94-95 0.31905001296465585 1106 96-97 0.3353851680959501 1106 98-99 0.6690967837343216 1106 100-101 0.3429594819522883 1107 1 -0.05916475684337286 1107 2 -0.017196303589827266 1107 3 -0.09079402391616043 1107 4 0.0230986008700782 1107 5 0.007678171581758875 1107 6 0.07911201600002471 1107 7 0.0866107110028338 1107 8 -0.04166781347478832 1107 9 0.0080665567772229 1107 10-11 0.03842112529400765 1107 12-13 -0.018018416616406796 1107 14-15 0.0833379557931977 1107 16-17 0.14123133027216284 1107 18-19 0.01399705115063199 1107 20-21 0.07148313874074574 1107 22-23 0.1807891720016528 1107 24-25 -0.03593237298453289 1107 26-27 -0.006393386301908777 1107 28-29 -0.038457061499308054 1107 30-31 0.017183178070020233 1107 32-33 0.02586412552342665 1107 34-35 -0.05364320040936832 1107 36-37 -0.16349683006561122 1107 38-39 -0.06155311200964775 1107 40-41 0.01965885415190627 1107 42-43 0.20802581767113537 1107 44-45 0.03610716370824463 1107 46-47 -0.09937857919559434 1107 48-49 -0.12512591312960097 1107 50-51 -0.08961868509420157 1107 52-53 -0.2299670910970022 1107 54-55 -0.40783141084423846 1107 56-57 -0.4139626515645318 1107 58-59 -0.4489369310855764 1107 60-61 -0.3422474478661002 1107 62-63 -0.45837902472501213 1107 64-65 -0.32114706322388287 1107 66-67 -0.33375983470946835 1107 68-69 -0.34265466767113395 1107 70-71 -0.29676775731280713 1107 72-73 -0.24598271869687238 1107 74-75 -0.4157383197755493 1107 76-77 -0.03112883244526543 1107 78-79 0.10955944628728531 1107 80-81 9.241761036768992E-4 1107 82-83 -0.07681219285177221 1107 84-85 -0.1192631522190446 1107 86-87 -0.1796808688001157 1107 88-89 0.007918754967100483 1107 90-91 -0.0705163712928325 1107 92-93 -0.3189949071716782 1107 94-95 -0.20234376633842288 1107 96-97 -0.308069014390437 1107 98-99 0.028469883678923225 1107 100-101 -0.35460912271458156 1108 1 0.0841461997617543 1108 2 0.06061855842735753 1108 3 0.0032513268138174567 1108 4 0.0495863168033992 1108 5 0.07353704677261419 1108 6 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-0.36012839004220254 2316 90-91 -0.6112414670499788 2316 92-93 -0.7774820919608132 2316 94-95 -0.3948833246247361 2316 96-97 -0.6033413437753268 2316 98-99 -0.6396916037323344 2316 100-101 -0.12041274425477155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 14.0 6 23.0 7 63.0 8 187.0 9 357.0 10 553.0 11 1015.0 12 1474.0 13 2129.0 14 2549.0 15 3547.0 16 4786.0 17 7128.0 18 7194.0 19 4387.0 20 4033.0 21 4040.0 22 4046.0 23 4333.0 24 5077.0 25 6140.0 26 7026.0 27 8749.0 28 10821.0 29 12992.0 30 16234.0 31 20189.0 32 25037.0 33 33100.0 34 46463.0 35 70104.0 36 115624.0 37 199208.0 38 175862.0 39 16273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7169606486349 18.809018489811287 14.647588402423578 22.82643245913023 2 15.829397975544737 20.708145094083076 39.502950909152936 23.959506021219255 3 20.00441052482809 24.59537699021887 29.405529482287236 25.99468300266581 4 13.454293804309156 16.467827536295452 36.03739930113894 34.040479358256455 5 14.255254860105952 37.43305018251288 32.905317484685 15.40637747269617 6 32.465483378900636 34.98752381927083 17.204701960612308 15.342290841216233 7 28.997738692242837 32.952225000121835 19.956162794664483 18.09387351297084 8 26.648951464732857 34.47495260513375 19.34063872197124 19.535457208162153 9 27.10060382765326 17.20043764102714 20.047419233787057 35.651539297532544 10-11 25.692830094887203 26.386695810245087 26.118165514082005 21.802308580785706 12-13 26.87733623794416 23.638768269563478 27.067159378335308 22.416736114157054 14-15 23.49930307815272 24.86018919235043 25.999449285533267 25.641058443963583 16-17 22.569974322188006 28.268072702018586 26.42323004334464 22.738722932448773 18-19 22.476585811007563 26.795433029997696 28.85102033108218 21.876960827912566 20-21 23.528687905414955 25.59401971821376 28.72774634365055 22.149546032720732 22-23 22.96183799484383 25.705196621684184 28.646846109234808 22.686119274237175 24-25 22.95466756989567 25.895421189411266 28.8430586810501 22.306852559642966 26-27 22.78031981548685 25.876921783554035 28.982844443740486 22.359913957218634 28-29 22.328342861041666 26.41995750300939 29.007546627288722 22.244153008660223 30-31 22.579271498447437 26.129301684639277 28.73829028577277 22.553136531140513 32-33 22.379575614914884 26.095564620280616 28.810900575561305 22.713959189243194 34-35 23.025740600498253 26.05168834919337 28.53868523039449 22.38388581991389 36-37 23.092269509034637 25.78666518795934 28.69902801398841 22.422037289017613 38-39 22.770108387015007 26.036403608188373 28.793179929269368 22.40030807552725 40-41 22.790016706249062 26.020013379621787 28.77520407578861 22.414765838340536 42-43 22.601403117565166 26.225077604038148 28.259019279919823 22.91449999847686 44-45 22.69986639934351 26.217529648928178 28.165979168532385 22.916624783195925 46-47 22.96187317049397 26.479581113558066 27.683910094151976 22.874635621795985 48-49 23.25974255411176 26.40946445650651 27.593618071166183 22.737174918215544 50-51 22.961256781799374 26.64289204069872 27.593410034321725 22.80244114318019 52-53 23.17337648017759 26.78934113745381 27.248365240635845 22.788917141732753 54-55 22.715725835928477 26.54551125536695 27.40867045825597 23.330092450448607 56-57 22.897203093702938 26.90158194072839 27.125458718949663 23.075756246619004 58-59 23.167641016768243 26.473526838694898 27.23639544886307 23.122436695673787 60-61 22.984559576590115 26.470855233616902 27.750357786712204 22.794227403080775 62-63 22.98442958220983 26.38880596742141 27.603283933799833 23.023480516568927 64-65 22.614318324011524 26.55116598263408 27.552085268819624 23.282430424534766 66-67 22.636247164849063 26.62193783899206 27.317056231483562 23.424758764675314 68-69 22.507213108362333 26.59504732383932 27.487547640631245 23.4101919271671 70-71 22.692089321656407 26.605638161517803 27.231518438917885 23.470754077907902 72-73 22.668391888533122 26.446030771330125 27.26185602682379 23.623721313312963 74-75 22.515485571979276 26.936732117880414 26.981141960417364 23.56664034972294 76-77 22.65045603838781 26.568051672140758 27.128972784215087 23.652519505256343 78-79 22.553101795473335 26.64395190546306 27.24705257393009 23.55589372513352 80-81 22.5653106803569 26.87890429385511 27.156035290491037 23.39974973529695 82-83 22.5427120678154 26.75321273335912 27.10435177260031 23.599723426225165 84-85 22.380245722278268 26.539723964501366 27.05259977289452 24.02743054032584 86-87 22.413088608027245 26.466839379502304 27.218067031120025 23.902004981350427 88-89 22.469064216637033 26.464491513035206 26.99271340048039 24.073730869847374 90-91 22.34688017730581 26.36121288622816 27.198204925446795 24.093702011019232 92-93 22.318291250590914 26.37774073911624 27.160304301845596 24.143663708447253 94-95 22.20217992017194 26.245303156571193 27.124666773883842 24.427850149373022 96-97 22.03044967859214 26.13229868756427 27.227802388993673 24.60944924484992 98-99 21.944955156814082 26.157753466650586 26.993636300501127 24.90365507603421 100-101 22.091313263243567 25.611173587310248 27.484931593638557 24.812581555807625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 161.0 2 128.0 3 162.5 4 143.5 5 146.0 6 255.0 7 542.5 8 904.5 9 1035.5 10 916.0 11 840.0 12 886.5 13 987.0 14 1073.5 15 1112.5 16 1159.0 17 1261.0 18 1334.5 19 1370.5 20 1489.5 21 1676.5 22 1981.5 23 2333.0 24 2672.5 25 3214.0 26 3963.0 27 4931.5 28 5828.5 29 6771.0 30 7957.5 31 9155.5 32 10605.5 33 11918.5 34 12936.0 35 13919.5 36 15543.5 37 17337.5 38 18602.5 39 19330.5 40 20110.0 41 21728.5 42 23355.5 43 24870.0 44 26444.5 45 27830.5 46 28977.0 47 31502.5 48 38244.0 49 39184.5 50 34285.5 51 32392.5 52 31133.5 53 30866.0 54 30602.5 55 28704.0 56 25917.5 57 23254.0 58 21360.0 59 19048.0 60 16037.5 61 13436.5 62 11129.0 63 8715.5 64 6547.5 65 4877.0 66 3373.5 67 2643.5 68 2033.0 69 1408.5 70 1044.0 71 728.5 72 612.5 73 517.0 74 381.0 75 298.5 76 202.0 77 112.0 78 66.5 79 37.5 80 18.5 81 14.5 82 7.5 83 3.5 84 2.0 85 1.0 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3411455668133593 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014620524292001111 16-17 0.0020103220901501527 18-19 1.218377024333426E-4 20-21 0.0 22-23 0.0 24-25 6.09188512166713E-4 26-27 0.0011574581731167548 28-29 0.0 30-31 0.0029850237096168933 32-33 0.0 34-35 0.011635500582384217 36-37 0.02771807730358544 38-39 0.022661812652601723 40-41 0.013767660374967714 42-43 0.011148149772650847 44-45 0.004203400733950319 46-47 0.0020103220901501527 48-49 0.0010965393219000833 50-51 1.218377024333426E-4 52-53 1.218377024333426E-4 54-55 0.0 56-57 0.0 58-59 0.00560453431193376 60-61 0.011391825177517533 62-63 0.004995345799767046 64-65 0.002253997495016838 66-67 0.004934426948550375 68-69 0.004386157287600333 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21370333006808293 78-79 6.701073633833842E-4 80-81 0.004020644180300305 82-83 7.919450658167269E-4 84-85 0.0 86-87 0.1103849584046084 88-89 0.4026126876909806 90-91 0.38908870272087953 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001827565536500139 100-101 8.528639170333983E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 820764.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.79113841371312 #Duplication Level Percentage of deduplicated Percentage of total 1 80.48753210250992 50.539037688268785 2 11.780889304736187 14.794709019406445 3 3.489834310078937 6.573920076152745 4 1.4488228441518192 3.638929429763456 5 0.7523343675899314 2.3619965704366352 6 0.45352061532306687 1.7086245438133818 7 0.2863470129941162 1.25860384490868 8 0.21539910001161516 1.082012376241485 9 0.16023636770450653 0.905528154909987 >10 0.8381949371238722 9.579303660349925 >50 0.05656663303690729 2.397187277988686 >100 0.027989912081806692 3.203443503962734 >500 0.0013606207167832871 0.6229426440730851 >1k 9.718719405594908E-4 1.3337612097238782 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3483 0.4243607175753322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.32262623604349117 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1906 0.23222266083795096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1689 0.20578387940991563 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1202 0.1464489183248778 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 955 0.11635500582384219 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 869 0.10587696341457471 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2183770243334259E-4 0.0 5 0.0 0.0 0.0 1.2183770243334259E-4 0.0 6 0.0 0.0 0.0 1.2183770243334259E-4 0.0 7 0.0 0.0 0.0 1.2183770243334259E-4 0.0 8 0.0 0.0 0.0 1.2183770243334259E-4 0.0 9 0.0 0.0 0.0 1.2183770243334259E-4 0.0 10-11 0.0 0.0 0.0 1.2183770243334259E-4 0.0 12-13 0.0 0.0 0.0 1.2183770243334259E-4 0.0 14-15 0.0 0.0 0.0 1.2183770243334259E-4 6.0918851216671295E-5 16-17 0.0 0.0 0.0 1.2183770243334259E-4 1.2183770243334259E-4 18-19 0.0 0.0 0.0 1.827565536500139E-4 1.2183770243334259E-4 20-21 0.0 0.0 0.0 2.4367540486668518E-4 1.2183770243334259E-4 22-23 0.0 0.0 0.0 2.4367540486668518E-4 1.2183770243334259E-4 24-25 0.0 0.0 0.0 3.655131073000278E-4 1.2183770243334259E-4 26-27 0.0 0.0 0.0 0.001035620470683412 1.2183770243334259E-4 28-29 0.0 0.0 0.0 0.0036551310730002775 1.2183770243334259E-4 30-31 0.0 0.0 0.0 0.010843555516567491 1.2183770243334259E-4 32-33 0.0 0.0 0.0 0.027352564196285414 2.4367540486668518E-4 34-35 0.0 0.0 0.0 0.05111091617078722 2.4367540486668518E-4 36-37 0.0 0.0 0.0 0.0895507112885068 2.4367540486668518E-4 38-39 0.0 0.0 0.0 0.15223620919046157 2.4367540486668518E-4 40-41 0.0 0.0 0.0 0.22107451106530013 2.4367540486668518E-4 42-43 0.0 0.0 0.0 0.2862576818671384 2.4367540486668518E-4 44-45 0.0 0.0 0.0 0.3700820211412781 2.4367540486668518E-4 46-47 0.0 0.0 0.0 0.47078088220243575 2.4367540486668518E-4 48-49 0.0 0.0 0.0 0.5801302201363607 2.4367540486668518E-4 50-51 0.0 0.0 0.0 0.7014805717599699 2.4367540486668518E-4 52-53 0.0 0.0 0.0 0.8253895151346794 2.4367540486668518E-4 54-55 0.0 0.0 0.0 0.9644672524623399 2.4367540486668518E-4 56-57 0.0 0.0 0.0 1.1219424828574351 2.4367540486668518E-4 58-59 0.0 0.0 0.0 1.3000692038149821 2.4367540486668518E-4 60-61 0.0 0.0 0.0 1.501345088234864 2.4367540486668518E-4 62-63 0.0 0.0 0.0 1.7155357691126802 2.4367540486668518E-4 64-65 0.0 0.0 0.0 1.9359401728145973 2.4367540486668518E-4 66-67 0.0 0.0 0.0 2.1862557324638994 2.4367540486668518E-4 68-69 0.0 0.0 0.0 2.4786052994527052 2.4367540486668518E-4 70-71 0.0 0.0 0.0 2.7931293282843788 2.4367540486668518E-4 72-73 0.0 0.0 0.0 3.123614096134821 2.4367540486668518E-4 74-75 0.0 0.0 0.0 3.4754204619110975 3.045942560833565E-4 76-77 0.0 0.0 0.0 3.850802423108226 3.655131073000278E-4 78-79 0.0 0.0 0.0 4.280158486483325 3.655131073000278E-4 80-81 0.0 0.0 0.0 4.746614130249377 3.655131073000278E-4 82-83 0.0 0.0 0.0 5.22732478520013 3.655131073000278E-4 84-85 0.0 0.0 0.0 5.715589377701751 3.655131073000278E-4 86-87 0.0 0.0 0.0 6.239004147355391 4.264319585166991E-4 88-89 0.0 0.0 0.0 6.807559785760584 4.8735080973337036E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 65 0.0058714827 29.201807 5 GTACATG 4715 0.0 26.250206 1 TACATGG 4670 0.0 25.911135 2 ACATGGG 4760 0.0 24.324617 3 GTAAGGT 190 3.3160904E-7 22.477709 4 GTATAGG 215 4.3168257E-8 22.141273 1 CATGGGG 1975 0.0 22.10466 4 GAGTACT 3100 0.0 21.506897 12-13 TATAGGG 200 5.4337943E-7 21.35382 2 GGTATCA 5910 0.0 21.184067 1 TAAGGTG 315 1.4551915E-11 21.090197 5 GTGTAGC 455 0.0 20.92472 1 GTATCAA 7385 0.0 20.562754 1 GTACTTT 3255 0.0 20.191189 14-15 CATGGGA 2820 0.0 20.024467 4 AGTACTT 3195 0.0 19.902016 12-13 ATGGGGG 950 0.0 18.481672 5 GTAGCCC 315 6.881237E-9 18.07731 3 TATCAAC 8600 0.0 17.877619 2 CAACGCA 8545 0.0 17.715023 5 >>END_MODULE