FastQCFastQC Report
Fri 27 May 2016
SRR939146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808447
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.4008920807424605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24300.3005762900969389No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16660.20607411493888902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.18974651399535158No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11370.14064001721819736No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9460.11701447342868487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8840.10934544874308397No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG2053.274181E-1127.7194632
GTGTAGC4600.021.6732141
GAGTACT28800.020.70971512-13
GTATCAA66950.020.7059021
GTACATG44900.019.9837681
AGTACTT29850.019.98123412-13
TACATGG44100.019.8650932
GTATAGG2451.764256E-719.3774131
ACATGGG44650.019.3034213
GTACTTT30800.019.21123914-15
CGCGGAA1152.034667E-618.60998282-83
GGTATCA53250.018.276631
ATAGGGG1851.5561268E-417.9188733
TAGCCCT4550.017.693834
GTAGCCC3751.7826096E-1017.6799533
TACTTTT34050.017.65560714-15
ATCAACG78500.017.6156043
TATCAAC78850.017.5963822
CATGGGA25600.017.5738954
AGTACAT35950.017.5190352
Produced by FastQC (version 0.11.5)