FastQCFastQC Report
Fri 27 May 2016
SRR939146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808447
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31780.39309936211031765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25540.3159143394681408No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18410.22772055558373028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16440.2033528481149661No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11660.1442271416679139No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9430.11664339158905902No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8540.10563463034682545No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGG550.00259191834.510753
TCGCGGA1200.027.83977382-83
CGATCGT1202.3283064E-1023.72760824-25
GTAAGGT2453.0013325E-1023.2419364
GTACATG46650.023.1864781
GAGTACT31150.023.0025512-13
AATTCGC1253.8744474E-1022.78202678-79
TACATGG46700.022.6592252
CGCGGAA1305.8935257E-1022.02707382-83
ACATGGG47150.021.939763
TCGCGCT1306.330083E-1021.90240960-61
GTACTTT32650.021.36442614-15
GTAAGCG1005.437996E-721.35484794-95
ATGGGCG1353.8204412E-421.0899035
GTACACG1404.74814E-420.4213181
ATTCGCG1401.5697879E-920.36377180-81
GTATCAA74950.020.344121
TCTAAGC2801.5934347E-920.3366933
CAATTCG1452.4647306E-919.63967978-79
CCTCGCG1452.4701876E-919.63664458-59
Produced by FastQC (version 0.11.5)