##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939146_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 808447 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49233159378413 34.0 31.0 34.0 31.0 34.0 2 32.7018295571633 34.0 31.0 34.0 31.0 34.0 3 32.80895469956596 34.0 31.0 34.0 31.0 34.0 4 36.25839294350774 37.0 35.0 37.0 35.0 37.0 5 36.21926483739812 37.0 37.0 37.0 35.0 37.0 6 36.263251641727905 37.0 37.0 37.0 35.0 37.0 7 36.21708535006005 37.0 37.0 37.0 35.0 37.0 8 36.193626793098375 37.0 37.0 37.0 35.0 37.0 9 37.93676889146722 39.0 38.0 39.0 35.0 39.0 10-11 37.85702958882895 39.0 38.0 39.0 35.0 39.0 12-13 37.76950870001373 39.0 38.0 39.0 35.0 39.0 14-15 39.16503555582493 40.5 38.5 41.0 36.0 41.0 16-17 39.2629584870746 41.0 39.0 41.0 36.0 41.0 18-19 39.303481860901215 41.0 39.0 41.0 36.0 41.0 20-21 39.273329606022415 41.0 39.0 41.0 36.0 41.0 22-23 39.19997909572304 41.0 39.0 41.0 36.0 41.0 24-25 39.128455545014084 41.0 39.0 41.0 35.0 41.0 26-27 38.97641898603124 40.0 38.5 41.0 35.0 41.0 28-29 38.8238264227587 40.0 38.0 41.0 35.0 41.0 30-31 38.63650987634316 40.0 38.0 41.0 35.0 41.0 32-33 38.54823136210537 40.0 38.0 41.0 34.5 41.0 34-35 38.441275680409476 40.0 38.0 41.0 34.0 41.0 36-37 38.29079024351627 40.0 38.0 41.0 34.0 41.0 38-39 38.15797263147739 40.0 38.0 41.0 34.0 41.0 40-41 37.95924655543283 40.0 38.0 41.0 33.0 41.0 42-43 37.76128119715949 40.0 37.0 41.0 33.0 41.0 44-45 37.48278303958082 40.0 37.0 41.0 33.0 41.0 46-47 37.13061400438124 40.0 37.0 41.0 32.0 41.0 48-49 36.75639714168028 40.0 36.0 41.0 31.0 41.0 50-51 36.4798650993819 39.5 36.0 41.0 30.5 41.0 52-53 36.20199716246087 39.0 35.0 41.0 30.0 41.0 54-55 35.95103946207976 39.0 35.0 40.0 29.5 41.0 56-57 35.707335793193614 39.0 35.0 40.0 29.0 41.0 58-59 35.37009166958378 38.0 35.0 40.0 28.0 41.0 60-61 34.97587968042432 38.0 34.0 40.0 27.5 41.0 62-63 34.56995263758787 37.0 34.0 40.0 26.5 41.0 64-65 34.10784380423206 36.5 34.0 39.5 26.0 41.0 66-67 33.67813598170319 36.0 33.0 39.0 26.0 40.5 68-69 33.18835124627836 35.5 33.0 39.0 25.0 40.0 70-71 32.67308555786588 35.0 32.0 38.0 23.5 40.0 72-73 32.10095899916754 35.0 31.5 37.0 22.0 39.0 74-75 31.57117225990077 35.0 31.0 36.5 20.0 39.0 76-77 29.5825855003482 32.5 28.5 35.0 18.0 36.5 78-79 30.57604209057613 34.0 30.5 35.0 18.0 37.0 80-81 30.607324908126323 34.0 31.0 35.0 18.0 37.0 82-83 30.25686656020741 34.0 30.5 35.0 15.0 36.5 84-85 29.809956620532947 34.0 30.0 35.0 8.5 36.0 86-87 29.12664281022751 34.0 29.0 35.0 3.5 36.0 88-89 28.460584923934405 33.5 29.0 35.0 2.0 35.0 90-91 27.921041824634145 33.0 27.0 35.0 2.0 35.0 92-93 27.486989252232984 33.0 27.0 35.0 2.0 35.0 94-95 26.94970975215444 32.5 25.5 34.0 2.0 35.0 96-97 26.167195252131556 32.0 25.0 34.0 2.0 35.0 98-99 24.978158741389358 32.0 19.0 34.0 2.0 35.0 100-101 19.165985525334374 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22460636103881626 1101 2 0.204684781199731 1101 3 0.2578578401464 1101 4 0.1710349157267217 1101 5 0.17448922998181615 1101 6 0.11112138202367561 1101 7 0.14845677208925423 1101 8 0.17929765213590798 1101 9 0.164995307665464 1101 10-11 0.14305151314412257 1101 12-13 0.268245479273439 1101 14-15 0.347194319388592 1101 16-17 0.2834636408109432 1101 18-19 0.31194141157440214 1101 20-21 0.35809914019579736 1101 22-23 0.415099593478665 1101 24-25 0.3487796708680193 1101 26-27 0.2361057940628868 1101 28-29 0.3387263862667922 1101 30-31 0.35075890218034544 1101 32-33 0.4018763294317793 1101 34-35 0.36457468401020066 1101 36-37 0.3897762702458323 1101 38-39 0.40367888152428577 1101 40-41 0.42031370632813747 1101 42-43 0.5692152818173284 1101 44-45 0.47382762001621614 1101 46-47 0.5724604848846511 1101 48-49 0.6606284873596735 1101 50-51 0.528505373984899 1101 52-53 0.5601578504446962 1101 54-55 0.7633142001679332 1101 56-57 0.8657007122146183 1101 58-59 0.7615142076811239 1101 60-61 0.8002758999395425 1101 62-63 0.5887150210546679 1101 64-65 0.4196920788627523 1101 66-67 0.7506589424675028 1101 68-69 0.8434687004404609 1101 70-71 0.5886293233769564 1101 72-73 0.7758880684041642 1101 74-75 0.828207350731315 1101 76-77 0.9530569128432127 1101 78-79 1.0408455721856846 1101 80-81 1.2520762470644158 1101 82-83 1.0701377232232652 1101 84-85 1.1728347327058586 1101 86-87 1.126348074699738 1101 88-89 1.0468009951891482 1101 90-91 1.533652982194294 1101 92-93 1.4884218234423905 1101 94-95 1.1204693784413848 1101 96-97 1.3585415723970655 1101 98-99 1.5014503071529646 1101 100-101 0.2717789044247638 1102 1 -0.1267023070018709 1102 2 -0.10190128760168449 1102 3 -0.016049229342030458 1102 4 -0.07692919225095807 1102 5 -0.0397904999064167 1102 6 -0.03129052166103463 1102 7 0.03493275191465273 1102 8 -0.013374812215033671 1102 9 0.01359364208795455 1102 10-11 -0.05237736468560428 1102 12-13 0.1217157116834997 1102 14-15 0.1779911658505 1102 16-17 0.09230133916380368 1102 18-19 -0.006570962962705096 1102 20-21 0.0475962832444452 1102 22-23 -0.013202237986341459 1102 24-25 -0.07378312341472082 1102 26-27 0.035050984163255805 1102 28-29 0.1284493206323134 1102 30-31 0.013117101882073712 1102 32-33 -0.0911195317589204 1102 34-35 0.14797420477965062 1102 36-37 0.13833103825467674 1102 38-39 -0.06385785669183974 1102 40-41 -0.31023849796482494 1102 42-43 -0.12877731189234254 1102 44-45 -0.35510089755278074 1102 46-47 -0.32365665849911096 1102 48-49 -0.23608769421173292 1102 50-51 -0.33250101017033273 1102 52-53 0.06585499684460672 1102 54-55 -0.040354175642654866 1102 56-57 -0.02233988833163636 1102 58-59 -0.11417699022769057 1102 60-61 -0.34406297973337274 1102 62-63 -0.45402823871845754 1102 64-65 -0.05578086807000915 1102 66-67 -0.08446522181138505 1102 68-69 -0.10295508347012827 1102 70-71 -0.3447098746659023 1102 72-73 -0.5191759407112713 1102 74-75 -0.3940546677341423 1102 76-77 -0.16712983626451106 1102 78-79 -0.17077409302318003 1102 80-81 -0.005811787252511635 1102 82-83 0.05846485672081059 1102 84-85 0.1569288026467106 1102 86-87 0.21635460966178144 1102 88-89 0.2376344541948292 1102 90-91 0.44737053778336033 1102 92-93 -0.23302960838455178 1102 94-95 -0.12338314924842209 1102 96-97 -0.11618781314894022 1102 98-99 -0.3182559546811845 1102 100-101 -0.4199049374626682 1103 1 0.06467647002340016 1103 2 0.16268584446395096 1103 3 0.15175570032715058 1103 4 0.047903934865480835 1103 5 0.17171477490473563 1103 6 0.2379405606520706 1103 7 0.20600594805947736 1103 8 0.1255529684570149 1103 9 0.18289635763888157 1103 10-11 0.2622492470622504 1103 12-13 0.3462126509906156 1103 14-15 0.16912248259965423 1103 16-17 0.2964886275201408 1103 18-19 0.2613699070555313 1103 20-21 0.23070264232232773 1103 22-23 0.27903084680136914 1103 24-25 0.2750198689009835 1103 26-27 0.12351192776836228 1103 28-29 0.23153606197120524 1103 30-31 0.4136160925046397 1103 32-33 0.3593416072095579 1103 34-35 0.5113134532818648 1103 36-37 0.4411992168168126 1103 38-39 0.5096231930607047 1103 40-41 0.5553067286566957 1103 42-43 0.36193275257756596 1103 44-45 0.46227708173869786 1103 46-47 0.5440721608548884 1103 48-49 0.3916392529099184 1103 50-51 0.6429890262974993 1103 52-53 0.6631947988763827 1103 54-55 0.5791410542349169 1103 56-57 0.6019987252396035 1103 58-59 0.6714524054482709 1103 60-61 0.6699014315716525 1103 62-63 0.5929057773013469 1103 64-65 0.601144763604907 1103 66-67 0.3612176545887209 1103 68-69 0.3273286818333432 1103 70-71 0.5003160923828105 1103 72-73 0.1632568217268684 1103 74-75 0.2777023002262631 1103 76-77 0.34187519434241764 1103 78-79 0.390763335343582 1103 80-81 0.20042071144506934 1103 82-83 0.31750448292555333 1103 84-85 0.36936001845811717 1103 86-87 0.289039960664649 1103 88-89 0.5302664058749507 1103 90-91 0.7831961108492642 1103 92-93 0.8722020600186795 1103 94-95 0.4287910823222987 1103 96-97 0.2698420774475707 1103 98-99 0.30835986324014897 1103 100-101 -0.46857280344339003 1104 1 -0.04986962292858976 1104 2 -0.0059811626861971945 1104 3 0.04946701289099309 1104 4 0.019127760197449106 1104 5 -0.07289068985522817 1104 6 -0.009394092054776593 1104 7 0.08177203590421556 1104 8 -0.0018410560577919455 1104 9 -0.11780908158481651 1104 10-11 0.10468253357910129 1104 12-13 0.057493294504396886 1104 14-15 -0.10815343670576283 1104 16-17 0.04348811679491149 1104 18-19 0.10106602554378696 1104 20-21 0.23947275212825758 1104 22-23 0.16382240706693807 1104 24-25 0.15369868657207775 1104 26-27 0.07083540836080005 1104 28-29 0.09638539802840995 1104 30-31 0.016000540921389472 1104 32-33 0.08765972518708054 1104 34-35 0.20253217136153268 1104 36-37 0.34831904572385497 1104 38-39 0.3409242655836877 1104 40-41 0.14173101364136897 1104 42-43 0.04337587055509573 1104 44-45 0.021321706355657 1104 46-47 -0.05180852096329858 1104 48-49 -0.1024554866202152 1104 50-51 -0.22565882865653464 1104 52-53 -0.09510448610829769 1104 54-55 -0.06803559285395266 1104 56-57 -0.12475529672158103 1104 58-59 -0.33323988260076476 1104 60-61 -0.37317504953150404 1104 62-63 -0.4375063141184725 1104 64-65 -0.17898802495484034 1104 66-67 -0.1444721436748111 1104 68-69 -0.3927779320583866 1104 70-71 -0.4218222503138165 1104 72-73 -0.5512318146367718 1104 74-75 -0.3435311012037694 1104 76-77 -0.45042721883429593 1104 78-79 -0.2441559062294516 1104 80-81 0.005571056184596301 1104 82-83 -0.324939975705707 1104 84-85 -0.4861606020730349 1104 86-87 -0.5244256292229927 1104 88-89 -0.5453289082210624 1104 90-91 -0.45785023746534037 1104 92-93 -0.5216149726677379 1104 94-95 -0.39626956629874144 1104 96-97 -1.0912087397127976 1104 98-99 -0.9533389365555607 1104 100-101 -1.029612448489356 1105 1 -0.12839101416362553 1105 2 -0.08247724619708663 1105 3 0.029815588086052003 1105 4 0.04378209746004558 1105 5 0.011480525865913194 1105 6 0.03484269075252655 1105 7 0.010204137319057338 1105 8 3.0879755288992783E-4 1105 9 0.03692672784848128 1105 10-11 0.04218828185079104 1105 12-13 0.02442252944775447 1105 14-15 -0.03154142971585827 1105 16-17 0.06687496015067751 1105 18-19 0.1347576039425462 1105 20-21 0.10665510263486766 1105 22-23 0.3000363270816564 1105 24-25 0.2780826634563667 1105 26-27 0.0448685490631604 1105 28-29 0.13789845810167378 1105 30-31 0.2435161762203748 1105 32-33 0.05311931866931019 1105 34-35 0.113681135906198 1105 36-37 0.11142606321392634 1105 38-39 0.17353938430466087 1105 40-41 0.0673273010242994 1105 42-43 0.25516902474673486 1105 44-45 0.1616552210788953 1105 46-47 0.14515600836821108 1105 48-49 0.18221425601049646 1105 50-51 0.1827501263843132 1105 52-53 0.05151955393033347 1105 54-55 -0.08711123244498253 1105 56-57 -0.08652737391703624 1105 58-59 -0.1205643687052742 1105 60-61 -0.2715111164873818 1105 62-63 -0.3682041926230042 1105 64-65 -0.5783712354029049 1105 66-67 -0.5576061780418158 1105 68-69 -0.26855490678131844 1105 70-71 -0.08653801743983536 1105 72-73 -0.24538256497783806 1105 74-75 -0.17620753482272633 1105 76-77 -0.42500069707516985 1105 78-79 -0.09522106097292848 1105 80-81 -0.043654268401141394 1105 82-83 0.02236580935491972 1105 84-85 -0.06298816925117023 1105 86-87 -0.12142004956727348 1105 88-89 -0.30694233311797703 1105 90-91 0.16695719543316656 1105 92-93 -0.07979897867659602 1105 94-95 -0.21511278410459056 1105 96-97 -0.5047106634677192 1105 98-99 -0.48530603344842405 1105 100-101 -1.116739002438802 1106 1 -0.057064840137030615 1106 2 0.00359493537304445 1106 3 -0.003408069726326346 1106 4 0.06570139475852699 1106 5 0.12709579094751433 1106 6 0.02328481198896526 1106 7 0.1324765362947744 1106 8 0.07090724653188119 1106 9 -0.048470620970746836 1106 10-11 0.05966736203551193 1106 12-13 0.021513453129649918 1106 14-15 -0.04553326606344399 1106 16-17 -0.09722795001996332 1106 18-19 -0.03274774000329472 1106 20-21 -0.1374875314744628 1106 22-23 0.05756527193505434 1106 24-25 -0.1194904920615798 1106 26-27 -0.14056728346158565 1106 28-29 0.09249862881612358 1106 30-31 -0.07476960268252952 1106 32-33 -0.0750250371754646 1106 34-35 -0.03305486222615883 1106 36-37 0.006414791683113208 1106 38-39 0.0706191823655189 1106 40-41 -0.057744741931550436 1106 42-43 -0.11095327683420209 1106 44-45 0.08289038387773218 1106 46-47 0.33054442021317243 1106 48-49 0.2594534240329125 1106 50-51 0.11541549688573127 1106 52-53 0.06259319459830692 1106 54-55 0.13349432963598673 1106 56-57 0.10905921989201062 1106 58-59 -0.04395534856242733 1106 60-61 -0.1491995042572185 1106 62-63 -0.16590939649545078 1106 64-65 -0.20066004922931313 1106 66-67 -0.37746048712251223 1106 68-69 -0.4135636978458095 1106 70-71 -0.3347022899701315 1106 72-73 -0.13980133335335054 1106 74-75 -0.17082711153843988 1106 76-77 -0.23152888319769005 1106 78-79 -0.1431186833729967 1106 80-81 0.014550056364853958 1106 82-83 -0.0020977898017200403 1106 84-85 -0.1105447275311846 1106 86-87 -0.2360686623300019 1106 88-89 -0.047649703750717975 1106 90-91 0.04096683277439794 1106 92-93 0.24093534878873513 1106 94-95 0.09130779355224661 1106 96-97 -0.2640817193438707 1106 98-99 -0.44506593889888535 1106 100-101 -1.2884675226947273 1107 1 0.0621392703372976 1107 2 0.09039923333271815 1107 3 0.08142905237351528 1107 4 0.08476595647600504 1107 5 -0.039904656460713284 1107 6 0.06512990931800999 1107 7 0.1300974607964136 1107 8 0.15003458743564835 1107 9 0.15533803034200844 1107 10-11 0.10385158769970104 1107 12-13 0.12636854257578278 1107 14-15 0.03779740412566923 1107 16-17 0.1485500786851759 1107 18-19 0.22432113245695717 1107 20-21 0.19475835130457853 1107 22-23 0.22634292424914548 1107 24-25 0.017773287046587427 1107 26-27 0.1744285265248493 1107 28-29 0.1930838103715402 1107 30-31 0.2579867160709739 1107 32-33 0.3526288748058377 1107 34-35 0.3817049931828578 1107 36-37 0.5148374291191118 1107 38-39 0.5077858454813224 1107 40-41 0.36492997808323224 1107 42-43 0.180605976349149 1107 44-45 0.26472543227594514 1107 46-47 0.35904805274188334 1107 48-49 0.49522868306807055 1107 50-51 0.5346911571679556 1107 52-53 0.43161354812181685 1107 54-55 0.4817474845741643 1107 56-57 0.2944797481177872 1107 58-59 0.41096028302884946 1107 60-61 0.3661410941335319 1107 62-63 0.252099494855365 1107 64-65 0.25390493504037437 1107 66-67 0.3693508415378943 1107 68-69 0.04469595516425784 1107 70-71 0.21005007312650292 1107 72-73 0.1093757550843435 1107 74-75 0.112016558345303 1107 76-77 0.13049627818884701 1107 78-79 0.25280475300626293 1107 80-81 0.2293193215501006 1107 82-83 0.4282112769615303 1107 84-85 0.30363900101274766 1107 86-87 0.39383951245747895 1107 88-89 0.2852681790851399 1107 90-91 0.5268672595876431 1107 92-93 0.5988805732385281 1107 94-95 0.7407572328716157 1107 96-97 0.488410983640982 1107 98-99 0.294640120997002 1107 100-101 -0.9997897915515637 1108 1 -0.14553109904687744 1108 2 -0.13729695961720978 1108 3 -0.07157808678736899 1108 4 -0.08284774173660736 1108 5 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-1.2816254066765076 2316 96-97 -1.2142904059227178 2316 98-99 -1.4322939189532633 2316 100-101 0.011070457259322097 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 15.0 6 34.0 7 74.0 8 178.0 9 351.0 10 598.0 11 926.0 12 1605.0 13 2230.0 14 2745.0 15 3748.0 16 5127.0 17 7477.0 18 7416.0 19 4809.0 20 4430.0 21 4735.0 22 4961.0 23 5348.0 24 6065.0 25 7137.0 26 8306.0 27 9968.0 28 12068.0 29 15004.0 30 18031.0 31 22645.0 32 28894.0 33 37694.0 34 53060.0 35 80601.0 36 132116.0 37 198480.0 38 116812.0 39 4750.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.77314993081416 18.805134409336734 14.617217213735811 22.804498446113296 2 15.909020628439466 20.58762046244219 39.486942248533296 24.016416660585048 3 20.056973431777223 24.5588146161715 29.376817527927003 26.007394424124282 4 13.542508043198872 16.4655196939317 35.89487004095507 34.09710222191436 5 14.288135152953748 37.45427962500943 32.969755593131026 15.287829628905792 6 32.498110574966574 34.97953483654464 17.162782470588674 15.359572117900122 7 29.086260447499964 32.9478617645931 19.884667764244284 18.081210023662653 8 26.73619915714945 34.44233202671295 19.332250598987937 19.48921821714967 9 27.105178199684087 17.14410468466084 20.09989523122728 35.6508218844278 10-11 25.660680291967193 26.39134043419049 26.119275598771473 21.828703675070845 12-13 26.86879906784242 23.67551614391543 27.052855660296842 22.402829127945306 14-15 23.482571372025134 24.83672257681461 25.987890257780418 25.692815793379843 16-17 22.535310572854787 28.239675866969886 26.580912344676467 22.644101215498864 18-19 22.450327603417417 26.679671023579775 28.921129028866453 21.94887234413635 20-21 23.526155703466028 25.623386567084793 28.76533650319687 22.08512122625231 22-23 22.95227763848465 25.70762214467986 28.647394325169124 22.692705891666368 24-25 22.972810833610612 25.83063577451583 28.898307495729465 22.29824589614409 26-27 22.796140907946715 25.818857202585015 29.07202059303946 22.312981296428813 28-29 22.365411709116366 26.35781937467762 29.06714973275923 22.209619183446783 30-31 22.56857901631152 25.995705346176063 28.850623479337546 22.58509215817487 32-33 22.383842107151118 26.081054169290006 28.846479732128387 22.688623991430486 34-35 22.97690149487112 25.98971502936796 28.597513001568604 22.435870474192317 36-37 23.046734845879183 25.7697998916193 28.716678502762715 22.466786759738795 38-39 22.76030538506553 26.06889103058181 28.834479166537797 22.336324417814872 40-41 22.774985202132378 25.988704758264387 28.772188296912333 22.464121742690903 42-43 22.613637839633196 26.177200251611072 28.30228707536766 22.906874833388073 44-45 22.74174534866029 26.25079939586083 28.119375623899863 22.88807963157901 46-47 22.925249546489958 26.46352468065657 27.67787396719389 22.933351805659584 48-49 23.120602203900027 26.44593443715941 27.567428492616248 22.866034866324316 50-51 22.92270450730602 26.65766170280403 27.673626959476426 22.746006830413524 52-53 23.177586162110813 26.750114726135415 27.225532409669402 22.846766702084366 54-55 22.589112211437484 26.593270801920227 27.48609370806002 23.33152327858227 56-57 22.91771935434194 26.871289565852535 27.152198692183095 23.058792387622436 58-59 23.176359844539718 26.369987556250447 27.343098666811805 23.11055393239803 60-61 22.953731592521354 26.414430349997215 27.80804888457328 22.82378917290815 62-63 22.853149275147196 26.407030824798376 27.741885606847756 22.997934293206672 64-65 22.634070132274985 26.533420663149972 27.60265428216066 23.229854922414383 66-67 22.587029475540955 26.641787260045053 27.345741700301694 23.4254415641123 68-69 22.51657554241607 26.624897949085874 27.524615917468644 23.333910591029415 70-71 22.711473617285506 26.569307040915536 27.2055894889702 23.51362985282876 72-73 22.701426314897574 26.442673422005402 27.249529035298544 23.606371227798483 74-75 22.505680644494937 26.918276646459198 26.985813541271103 23.59022916777476 76-77 22.587384665859854 26.558314736460908 27.142127564743422 23.71217303293581 78-79 22.571564127037345 26.559373312458597 27.271411401640577 23.597651158863485 80-81 22.600781376110078 26.81027972819442 27.144934321484875 23.44400457421063 82-83 22.52677046238034 26.637058458996076 27.167396254794685 23.668774823828898 84-85 22.33250911933621 26.5364334334842 27.041846899054605 24.089210548124985 86-87 22.45988907504337 26.47240559057819 27.197045963646634 23.870659370731808 88-89 22.39274929725124 26.404410641286972 27.024618254947192 24.178221806514603 90-91 22.288663878849835 26.30946754271937 27.247980095658598 24.1538884827722 92-93 22.305481992016794 26.356644282185478 27.125649547836776 24.212224177960955 94-95 22.181863611259374 26.150338952546644 27.194873130132912 24.47292430606107 96-97 22.046157633091596 25.992180068699618 27.27921558246861 24.682446715740177 98-99 21.921329267093455 26.04603526149877 27.010911681739834 25.02172378966795 100-101 22.38450210929494 24.99160425780093 27.616580214745458 25.007313418158677 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 198.0 1 136.5 2 112.5 3 166.0 4 153.0 5 155.5 6 271.0 7 533.0 8 826.0 9 955.0 10 888.5 11 802.5 12 840.0 13 947.5 14 1007.5 15 1043.5 16 1137.0 17 1248.0 18 1302.5 19 1323.5 20 1445.0 21 1677.5 22 1979.5 23 2275.0 24 2644.0 25 3187.0 26 3927.5 27 4864.5 28 5773.5 29 6787.5 30 7903.5 31 9016.0 32 10371.5 33 11534.5 34 12707.5 35 13902.5 36 15382.0 37 17165.0 38 18476.5 39 19101.0 40 19724.0 41 21397.5 42 22957.0 43 24394.0 44 26015.5 45 27591.5 46 28890.0 47 31121.5 48 37495.5 49 38270.0 50 33609.5 51 31823.0 52 30646.0 53 30327.5 54 30176.0 55 28431.0 56 25548.5 57 23087.0 58 21074.0 59 18706.0 60 15922.0 61 13301.0 62 10888.0 63 8535.5 64 6362.5 65 4716.0 66 3408.5 67 2732.5 68 2048.0 69 1346.0 70 993.5 71 710.5 72 557.0 73 452.5 74 375.0 75 310.0 76 192.5 77 98.5 78 65.5 79 35.5 80 18.0 81 9.5 82 6.0 83 4.5 84 3.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41672490589983013 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.658576257936513E-4 16-17 0.0016698682783163276 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0022883380110260785 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010266597562981865 36-37 0.022883380110260785 38-39 0.01929625566054423 40-41 0.006617626139994334 42-43 0.006988707979620185 44-45 0.0029686547170068044 46-47 0.0037726653695294805 48-49 0.002040950117942178 50-51 4.947757861678007E-4 52-53 0.0 54-55 0.0 56-57 6.184697327097509E-5 58-59 0.0011132455188775517 60-61 0.0014843273585034022 62-63 8.658576257936513E-4 64-65 0.0021646440644841284 66-67 0.0017317152515873026 68-69 0.003339736556632655 70-71 1.2369394654195018E-4 72-73 0.0 74-75 0.0 76-77 0.09147167346777216 78-79 0.016513141863350348 80-81 1.8554091981292528E-4 82-83 0.0 84-85 0.0 86-87 0.10810850927766445 88-89 0.4198172545633789 90-91 0.4201264894297338 92-93 0.0 94-95 6.184697327097509E-5 96-97 0.0 98-99 1.8554091981292528E-4 100-101 1.8554091981292528E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 808447.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.17936171160079 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82764750430196 51.0663917797206 2 11.610602395970556 14.671008969292052 3 3.417937472361539 6.478293236218927 4 1.3920525629605207 3.5179596958737473 5 0.7460656332809462 2.3567975252825706 6 0.4429880515381227 1.6792621405226582 7 0.2921252367102276 1.291940019664173 8 0.19823390541669114 1.001943329105951 9 0.14580920468310668 0.8290919235199349 >10 0.8415126870118599 9.682547985712876 >50 0.05485604482372064 2.348285784336229 >100 0.02801435088268884 3.2430541627130918 >500 0.0011754273044255873 0.5483066141095709 >1k 9.795227536879894E-4 1.285116833927692 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3178 0.39309936211031765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2554 0.3159143394681408 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1841 0.22772055558373028 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1644 0.2033528481149661 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1166 0.1442271416679139 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 943 0.11664339158905902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 854 0.10563463034682545 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.2369394654195018E-4 0.0 16-17 0.0 0.0 0.0 1.8554091981292525E-4 0.0 18-19 0.0 0.0 0.0 2.4738789308390035E-4 0.0 20-21 0.0 0.0 0.0 2.4738789308390035E-4 0.0 22-23 0.0 0.0 0.0 2.4738789308390035E-4 0.0 24-25 0.0 0.0 0.0 5.566227594387759E-4 0.0 26-27 0.0 0.0 0.0 0.0011750924921485268 1.2369394654195018E-4 28-29 0.0 0.0 0.0 0.004267441155697281 1.2369394654195018E-4 30-31 0.0 0.0 0.0 0.013297099253259646 1.2369394654195018E-4 32-33 0.0 0.0 0.0 0.03011947598296487 1.2369394654195018E-4 34-35 0.0 0.0 0.0 0.05250808030705785 1.2369394654195018E-4 36-37 0.0 0.0 0.0 0.09307969477281752 1.2369394654195018E-4 38-39 0.0 0.0 0.0 0.1572150060548187 1.2369394654195018E-4 40-41 0.0 0.0 0.0 0.22283464469532327 1.2369394654195018E-4 42-43 0.0 0.0 0.0 0.29383497001040265 1.2369394654195018E-4 44-45 0.0 0.0 0.0 0.382399835734439 1.2369394654195018E-4 46-47 0.0 0.0 0.0 0.48296301427304444 1.2369394654195018E-4 48-49 0.0 0.0 0.0 0.5888450325129538 1.2369394654195018E-4 50-51 0.0 0.0 0.0 0.712477132081633 1.2369394654195018E-4 52-53 0.0 0.0 0.0 0.8395108151802159 1.2369394654195018E-4 54-55 0.0 0.0 0.0 0.9816351597569166 1.2369394654195018E-4 56-57 0.0 0.0 0.0 1.1438597706466842 1.2369394654195018E-4 58-59 0.0 0.0 0.0 1.332864120962784 1.2369394654195018E-4 60-61 0.0 0.0 0.0 1.542092431538493 1.2369394654195018E-4 62-63 0.0 0.0 0.0 1.7544130907777504 1.2369394654195018E-4 64-65 0.0 0.0 0.0 1.9798453083504546 1.2369394654195018E-4 66-67 0.0 0.0 0.0 2.241581699233221 1.2369394654195018E-4 68-69 0.0 0.0 0.0 2.532571708473159 1.2369394654195018E-4 70-71 0.0 0.0 0.0 2.8539904285624162 3.7108183962585056E-4 72-73 0.0 0.0 0.0 3.1906853510496047 3.7108183962585056E-4 74-75 0.0 0.0 0.0 3.543584180533789 3.7108183962585056E-4 76-77 0.0 0.0 0.0 3.9270354148138344 3.7108183962585056E-4 78-79 0.0 0.0 0.0 4.363427658213835 3.7108183962585056E-4 80-81 0.0 0.0 0.0 4.843607558689685 3.7108183962585056E-4 82-83 0.0 0.0 0.0 5.331765718717492 3.7108183962585056E-4 84-85 0.0 0.0 0.0 5.83903459348603 4.3292881289682566E-4 86-87 0.0 0.0 0.0 6.365104948128944 4.947757861678007E-4 88-89 0.0 0.0 0.0 6.930571824745469 4.947757861678007E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTACGG 55 0.002591918 34.51075 3 TCGCGGA 120 0.0 27.839773 82-83 CGATCGT 120 2.3283064E-10 23.727608 24-25 GTAAGGT 245 3.0013325E-10 23.241936 4 GTACATG 4665 0.0 23.186478 1 GAGTACT 3115 0.0 23.00255 12-13 AATTCGC 125 3.8744474E-10 22.782026 78-79 TACATGG 4670 0.0 22.659225 2 CGCGGAA 130 5.8935257E-10 22.027073 82-83 ACATGGG 4715 0.0 21.93976 3 TCGCGCT 130 6.330083E-10 21.902409 60-61 GTACTTT 3265 0.0 21.364426 14-15 GTAAGCG 100 5.437996E-7 21.354847 94-95 ATGGGCG 135 3.8204412E-4 21.089903 5 GTACACG 140 4.74814E-4 20.421318 1 ATTCGCG 140 1.5697879E-9 20.363771 80-81 GTATCAA 7495 0.0 20.34412 1 TCTAAGC 280 1.5934347E-9 20.336693 3 CAATTCG 145 2.4647306E-9 19.639679 78-79 CCTCGCG 145 2.4701876E-9 19.636644 58-59 >>END_MODULE