##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 984892 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.029494604484555 33.0 31.0 34.0 30.0 34.0 2 32.10924040402399 34.0 31.0 34.0 30.0 34.0 3 32.1678711980603 34.0 31.0 34.0 30.0 34.0 4 35.57976712167425 37.0 35.0 37.0 33.0 37.0 5 35.64880007147992 37.0 35.0 37.0 35.0 37.0 6 35.789666278129985 37.0 36.0 37.0 35.0 37.0 7 35.73896934892354 37.0 36.0 37.0 35.0 37.0 8 35.74745048188025 37.0 36.0 37.0 35.0 37.0 9 37.3649354446985 39.0 37.0 39.0 35.0 39.0 10-11 37.29079533593531 39.0 37.0 39.0 34.0 39.0 12-13 37.26061436177774 39.0 37.0 39.0 34.0 39.0 14-15 38.42213765570235 40.0 38.0 41.0 34.0 41.0 16-17 38.426436096546624 40.0 38.0 41.0 34.0 41.0 18-19 38.461173407845735 40.0 38.0 41.0 34.0 41.0 20-21 38.37128487184381 40.0 38.0 41.0 34.0 41.0 22-23 38.3036353224516 40.0 38.0 41.0 34.0 41.0 24-25 38.30398256864712 40.0 38.0 41.0 34.0 41.0 26-27 38.1768600008935 40.0 38.0 41.0 34.0 41.0 28-29 38.10187665246545 40.0 38.0 41.0 34.0 41.0 30-31 37.99670573017143 40.0 38.0 41.0 34.0 41.0 32-33 37.74199353837781 40.0 37.0 41.0 33.0 41.0 34-35 37.36772255232046 40.0 37.0 41.0 32.5 41.0 36-37 37.049098784435245 40.0 36.5 41.0 31.5 41.0 38-39 36.794716578061355 39.5 36.0 41.0 30.5 41.0 40-41 36.78642074460956 40.0 36.0 41.0 31.0 41.0 42-43 36.5555294387608 40.0 36.0 41.0 30.0 41.0 44-45 36.24021466313057 40.0 36.0 41.0 30.0 41.0 46-47 35.7719927667196 39.0 35.0 41.0 28.0 41.0 48-49 35.592371549367854 39.5 35.5 41.0 26.5 41.0 50-51 34.55518777693392 38.0 34.0 40.0 24.5 40.5 52-53 34.81293075789021 38.5 35.0 39.5 25.0 40.5 54-55 35.40342900541379 39.5 36.0 41.0 26.0 41.0 56-57 35.25150879487294 39.5 35.0 41.0 24.5 41.0 58-59 35.10018306575746 39.0 35.0 41.0 24.0 41.0 60-61 34.68989950167125 39.0 35.0 41.0 22.0 41.0 62-63 34.42155028165524 39.0 35.0 40.0 22.0 41.0 64-65 34.06057720034278 38.0 34.0 40.0 21.0 41.0 66-67 33.55070555959435 37.5 34.0 40.0 18.5 41.0 68-69 33.06984775995744 37.0 33.5 39.0 15.5 41.0 70-71 32.494878626285924 36.0 32.5 39.0 10.5 40.5 72-73 31.985271989213032 36.0 32.0 38.5 7.5 40.0 74-75 31.518411155740935 35.0 32.0 37.0 3.5 39.5 76-77 31.074500554375504 35.0 31.5 37.0 2.0 39.0 78-79 30.579572684111557 35.0 31.0 36.0 2.0 39.0 80-81 30.056071630188896 35.0 31.0 36.0 2.0 37.5 82-83 29.55384499011059 34.5 29.5 35.5 2.0 37.0 84-85 29.13428071301219 34.0 29.0 35.0 2.0 37.0 86-87 28.88791766000739 34.0 29.0 35.0 2.0 36.0 88-89 28.38894163014828 34.0 29.0 35.0 2.0 36.0 90-91 27.925143569041072 34.0 28.5 35.0 2.0 35.5 92-93 27.459535664824166 34.0 27.0 35.0 2.0 35.0 94-95 26.95183685114713 34.0 25.5 35.0 2.0 35.0 96-97 26.33846198364897 34.0 25.0 35.0 2.0 35.0 98-99 25.710501252929255 33.5 22.0 35.0 2.0 35.0 100-101 23.21127544949091 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14556826062026573 1101 2 0.2267710429277301 1101 3 0.24979002524656124 1101 4 0.38810499136399557 1101 5 0.29257187912055826 1101 6 0.37059653728298514 1101 7 0.2225434985672763 1101 8 0.28437914084035043 1101 9 0.3532373967874136 1101 10-11 0.34254307065421585 1101 12-13 0.2980921185978289 1101 14-15 0.3401574094960509 1101 16-17 0.5313715024694616 1101 18-19 0.5068815236020896 1101 20-21 0.3771156237383835 1101 22-23 0.5205962346776971 1101 24-25 0.5634957200201782 1101 26-27 0.5393370585172335 1101 28-29 0.42548927636372724 1101 30-31 0.4640541603061692 1101 32-33 0.6099079213618737 1101 34-35 0.6120121985347069 1101 36-37 0.6064880818416327 1101 38-39 0.6564952898914456 1101 40-41 0.476915993248447 1101 42-43 0.39737121507359063 1101 44-45 0.4108352260084658 1101 46-47 0.40631371317211773 1101 48-49 0.5064700000436702 1101 50-51 0.39405399978875266 1101 52-53 0.5506655548288961 1101 54-55 0.6463983535417199 1101 56-57 0.5968281048945556 1101 58-59 0.541163027687702 1101 60-61 0.38777728582232385 1101 62-63 0.37909895750211575 1101 64-65 0.4217750277443315 1101 66-67 0.13857367233703854 1101 68-69 0.28251250174673004 1101 70-71 0.38687375255457823 1101 72-73 0.3219371114630931 1101 74-75 0.15485578584643278 1101 76-77 0.14208977463358963 1101 78-79 0.26560921076028166 1101 80-81 0.29931366752479605 1101 82-83 0.14980092031724368 1101 84-85 -0.09697767142179714 1101 86-87 -0.06301939457381422 1101 88-89 0.16855729369519068 1101 90-91 -0.22051347517772513 1101 92-93 -0.32713764574578263 1101 94-95 -0.3384267392893676 1101 96-97 -0.5274431147267116 1101 98-99 -0.4034317178027145 1101 100-101 -0.6644902304099389 1102 1 0.23594341812137998 1102 2 0.34894545835906854 1102 3 0.42242576653247 1102 4 0.4594754978643678 1102 5 0.4968123608271995 1102 6 0.5226105193176949 1102 7 0.3979489369284508 1102 8 0.43036598332762566 1102 9 0.5699386120863608 1102 10-11 0.475519014354461 1102 12-13 0.45512000718284185 1102 14-15 0.6616077552727049 1102 16-17 0.6628261450507935 1102 18-19 0.6112880710033934 1102 20-21 0.776554426207305 1102 22-23 0.833023290611635 1102 24-25 0.6250721247559525 1102 26-27 0.7833534676659397 1102 28-29 0.6968252342318166 1102 30-31 0.6646263901736091 1102 32-33 0.6799182569190947 1102 34-35 0.5663034870535242 1102 36-37 0.6890114094265343 1102 38-39 0.6655809995319615 1102 40-41 0.6912292883750482 1102 42-43 0.6299270590182999 1102 44-45 0.37828257563482026 1102 46-47 0.4655544561709348 1102 48-49 0.7653771600299706 1102 50-51 0.7259182904385568 1102 52-53 0.6348306682587292 1102 54-55 0.5768118919603822 1102 56-57 0.4597992292057569 1102 58-59 0.3167682094018929 1102 60-61 0.4737017665767098 1102 62-63 0.47932483007282656 1102 64-65 0.48926824866396856 1102 66-67 0.5768245244398571 1102 68-69 0.5969761850373914 1102 70-71 0.5667676098658205 1102 72-73 0.500971607836938 1102 74-75 0.38402499285371405 1102 76-77 0.4068382663970773 1102 78-79 0.536972749469264 1102 80-81 0.873532258753503 1102 82-83 0.6189757015576269 1102 84-85 0.47412863965525176 1102 86-87 0.44611567025567567 1102 88-89 0.681150124999153 1102 90-91 0.8499541216890911 1102 92-93 0.6519328508835507 1102 94-95 0.5368483433079909 1102 96-97 0.45852114050348547 1102 98-99 0.5516318342612045 1102 100-101 0.7493820615869708 1103 1 0.32554014518158425 1103 2 0.41283736228604084 1103 3 0.5449773236836748 1103 4 0.40444502278713657 1103 5 0.4745697291164177 1103 6 0.388945995647326 1103 7 0.33118486133913194 1103 8 0.40319167701830594 1103 9 0.47510128498719695 1103 10-11 0.46952975716707357 1103 12-13 0.4932959555282821 1103 14-15 0.5001218517353649 1103 16-17 0.5085359777857548 1103 18-19 0.568284814762265 1103 20-21 0.5834788090522025 1103 22-23 0.6593417899576082 1103 24-25 0.5840365287524989 1103 26-27 0.6424876414991161 1103 28-29 0.5780113965985763 1103 30-31 0.3895978632748296 1103 32-33 0.5007373268030406 1103 34-35 0.5429309118399246 1103 36-37 0.5271901408840449 1103 38-39 0.5679647350249866 1103 40-41 0.338579765678773 1103 42-43 0.44632515536963524 1103 44-45 0.34043085984622934 1103 46-47 0.3227529755553178 1103 48-49 0.48450688088382776 1103 50-51 0.5164223120355018 1103 52-53 0.6140658690602692 1103 54-55 0.5560568336541891 1103 56-57 0.49175492206151716 1103 58-59 0.6498705444329218 1103 60-61 0.6626263720705694 1103 62-63 0.6825017525074912 1103 64-65 0.5486459448037948 1103 66-67 0.6340007789276285 1103 68-69 0.5180165123313714 1103 70-71 0.6820858587317034 1103 72-73 0.5455334068405833 1103 74-75 0.47833631177052993 1103 76-77 0.4497677701681937 1103 78-79 0.387841080436953 1103 80-81 0.30176136453959757 1103 82-83 0.1720162515005761 1103 84-85 0.1716405374593748 1103 86-87 0.20571044913742398 1103 88-89 0.2707899902965316 1103 90-91 0.21750025177174948 1103 92-93 0.014580703712585574 1103 94-95 -0.0035387875817711745 1103 96-97 0.2115463833300133 1103 98-99 0.20177377297707366 1103 100-101 0.14371286228831792 1104 1 0.4157377430118814 1104 2 0.3734986046985398 1104 3 0.42711602961161077 1104 4 0.3941001952009273 1104 5 0.3241241653814342 1104 6 0.3223439034350051 1104 7 0.45490951586580053 1104 8 0.4617994517826105 1104 9 0.4218144452609991 1104 10-11 0.5280858723678961 1104 12-13 0.44704127388034465 1104 14-15 0.5591170732257424 1104 16-17 0.5914043750477944 1104 18-19 0.6842088752039572 1104 20-21 0.668626684552649 1104 22-23 0.556603833093348 1104 24-25 0.4808723804895507 1104 26-27 0.6546147240252722 1104 28-29 0.6571021882813781 1104 30-31 0.620561314133667 1104 32-33 0.5848520110339663 1104 34-35 0.5696389061573726 1104 36-37 0.6169938345428392 1104 38-39 0.6542103762493241 1104 40-41 0.7671862489539905 1104 42-43 0.660137631154214 1104 44-45 0.7223177254022133 1104 46-47 0.7343699198921314 1104 48-49 0.914895619098985 1104 50-51 1.0998525070503575 1104 52-53 1.0729771437599993 1104 54-55 1.0080263545117347 1104 56-57 1.046042665830079 1104 58-59 1.1545909370456044 1104 60-61 1.1638274569214033 1104 62-63 1.342575367385443 1104 64-65 1.1934089565563397 1104 66-67 1.1459147657544406 1104 68-69 1.3951765278292356 1104 70-71 1.3428891649437702 1104 72-73 1.168928731650091 1104 74-75 1.2564778589744456 1104 76-77 1.1013304719633652 1104 78-79 1.191424962653148 1104 80-81 1.0688283928232067 1104 82-83 0.9792744201690482 1104 84-85 1.1107339121203594 1104 86-87 0.9995435887473931 1104 88-89 1.0895553267294389 1104 90-91 0.8199992831549352 1104 92-93 0.850968408736474 1104 94-95 0.6909659263533996 1104 96-97 0.8508205664631667 1104 98-99 0.9418745091258565 1104 100-101 0.9759484298977696 1105 1 0.18090016919568797 1105 2 0.16367723070873552 1105 3 0.2092901074441329 1105 4 0.2700523170725262 1105 5 0.24704355749059204 1105 6 0.2524107812088445 1105 7 0.2608710519761317 1105 8 0.24714625033012538 1105 9 0.20666607930385794 1105 10-11 0.2198905941588123 1105 12-13 0.23879831316340017 1105 14-15 0.41169865310617126 1105 16-17 0.3182416798857375 1105 18-19 0.3506813477253772 1105 20-21 0.3201109554382029 1105 22-23 0.4253155495413665 1105 24-25 0.1584658809969497 1105 26-27 0.32566838518603447 1105 28-29 0.3508160443291146 1105 30-31 0.3118947126738547 1105 32-33 0.3249615585391865 1105 34-35 0.39427670249192914 1105 36-37 0.35384079814518543 1105 38-39 0.5177020807155799 1105 40-41 0.35308078502763607 1105 42-43 0.26084431037320144 1105 44-45 0.13105034879598776 1105 46-47 0.2996362694905059 1105 48-49 0.4782638380961899 1105 50-51 0.4318407453650863 1105 52-53 0.3325980066911285 1105 54-55 0.2722369644810456 1105 56-57 0.2940937961500367 1105 58-59 0.26202268381481275 1105 60-61 0.4271192616431989 1105 62-63 0.6009625982543696 1105 64-65 0.6229177044625942 1105 66-67 0.5132210708491343 1105 68-69 0.21940107576238432 1105 70-71 0.19082536819351503 1105 72-73 0.24835006162526696 1105 74-75 0.4921150317685239 1105 76-77 0.5216474995091822 1105 78-79 0.10953820249616086 1105 80-81 -0.0016775308114240772 1105 82-83 0.06856297269927936 1105 84-85 0.2334937512224613 1105 86-87 -0.03348632896971537 1105 88-89 0.020482934901483674 1105 90-91 0.12721095681095917 1105 92-93 0.22923618881007712 1105 94-95 -0.013829833571286088 1105 96-97 0.15603752187390896 1105 98-99 0.009923560977522783 1105 100-101 -0.4595084052063356 1106 1 0.04582952126031614 1106 2 0.30209225560615494 1106 3 0.23358956708274548 1106 4 0.21199156900284777 1106 5 0.17212765941644648 1106 6 0.12296971861012906 1106 7 0.12404593878762427 1106 8 0.16970410770392164 1106 9 0.1469679075864434 1106 10-11 0.12149626592547236 1106 12-13 0.24830128034192 1106 14-15 0.2612984519336692 1106 16-17 0.2096780585025826 1106 18-19 0.24421114871989147 1106 20-21 0.29673392270943566 1106 22-23 0.37658684155321964 1106 24-25 0.44834375213761035 1106 26-27 0.4786284369161109 1106 28-29 0.3795005082727627 1106 30-31 0.28698686845275034 1106 32-33 0.2470889113941368 1106 34-35 0.39080833095784584 1106 36-37 0.11517442350312024 1106 38-39 0.28262633393799774 1106 40-41 0.31492290401376266 1106 42-43 0.08885572714444834 1106 44-45 0.2722291779395789 1106 46-47 0.2610861252743888 1106 48-49 0.23190690291753668 1106 50-51 0.3114408570760787 1106 52-53 0.29272621939628607 1106 54-55 0.3440466247011571 1106 56-57 0.5787256539479699 1106 58-59 0.572947185026365 1106 60-61 0.5727839131083599 1106 62-63 0.46368496281926497 1106 64-65 0.41509411049730716 1106 66-67 0.5292157912297881 1106 68-69 0.35800035427620713 1106 70-71 0.3680779265955323 1106 72-73 0.3114656164546261 1106 74-75 0.3092294115465144 1106 76-77 0.26121428333333796 1106 78-79 0.30789359411621575 1106 80-81 0.42564774403355443 1106 82-83 0.37849783498998235 1106 84-85 0.5077813328763625 1106 86-87 0.4987771745606544 1106 88-89 0.7688400068218151 1106 90-91 0.8647295125407268 1106 92-93 0.7256067402150954 1106 94-95 0.5535982988271044 1106 96-97 0.37868310251036164 1106 98-99 0.3416878415019191 1106 100-101 0.534372404721573 1107 1 0.10670612998578832 1107 2 0.03493214116778631 1107 3 0.09993787306999735 1107 4 0.08689680537042221 1107 5 0.06597095646884554 1107 6 -0.02505897220773079 1107 7 0.02294893692845079 1107 8 0.0036105353857323053 1107 9 0.10277759513721207 1107 10-11 0.13396332428182234 1107 12-13 0.16864906287291603 1107 14-15 0.21820581822669993 1107 16-17 0.013764401709387641 1107 18-19 0.12150296204455202 1107 20-21 -0.07839412100818777 1107 22-23 0.16451542136224617 1107 24-25 0.13422769376321497 1107 26-27 -0.005463118290478519 1107 28-29 -0.09895261080450979 1107 30-31 -0.0771260311339006 1107 32-33 -0.081053292717705 1107 34-35 -0.1431396122200752 1107 36-37 -0.04699040655409448 1107 38-39 0.15748475255860228 1107 40-41 0.0741742036292905 1107 42-43 0.17729388710546345 1107 44-45 0.36087942793506755 1107 46-47 0.3008298798997515 1107 48-49 0.3638184433229483 1107 50-51 0.4235575156201534 1107 52-53 0.4787319999778603 1107 54-55 0.5017513592727312 1107 56-57 0.4796993502711331 1107 58-59 0.4265593716294944 1107 60-61 0.38630769878010085 1107 62-63 0.4423242247459527 1107 64-65 0.39483725592788943 1107 66-67 0.2493426842461588 1107 68-69 0.43913720198654005 1107 70-71 0.40393468008373645 1107 72-73 0.4245490896771287 1107 74-75 0.5386860098028627 1107 76-77 0.5986512203922345 1107 78-79 0.512154347532217 1107 80-81 0.3189771740077454 1107 82-83 0.606869164027362 1107 84-85 0.6116135064833372 1107 86-87 0.5536368566963539 1107 88-89 0.6352966141032717 1107 90-91 0.4263766398489004 1107 92-93 0.41487171286902225 1107 94-95 0.23221759270266418 1107 96-97 0.12513736326377511 1107 98-99 0.2484387349875945 1107 100-101 0.46654307853612664 1108 1 -0.07924260276332973 1108 2 -0.07272187048148027 1108 3 0.08704344232714334 1108 4 -0.05172023823560323 1108 5 -0.03867890972205856 1108 6 0.15967301603806305 1108 7 0.07903725093380842 1108 8 0.11939038880792197 1108 9 0.09609697909061055 1108 10-11 0.14586511889797293 1108 12-13 0.20841463457885112 1108 14-15 0.14408825862479802 1108 16-17 0.2110661368548321 1108 18-19 0.1255123155629505 1108 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1.1263833342971772 2201 82-83 1.197208933581809 2201 84-85 1.004451162309362 2201 86-87 1.0829672963518675 2201 88-89 0.8365899209576497 2201 90-91 0.9178602805740539 2201 92-93 1.022058635280441 2201 94-95 0.9821414001665829 2201 96-97 1.1544911522448622 2201 98-99 1.4299920304376705 2201 100-101 1.4236712589600486 2202 1 0.27077479233020085 2202 2 0.2731593579283995 2202 3 0.35747096335646233 2202 4 0.28513311234053873 2202 5 0.3751450941013488 2202 6 0.33519829515024213 2202 7 0.27665957006875885 2202 8 0.1760503668561384 2202 9 0.3949833416887003 2202 10-11 0.42158789381952033 2202 12-13 0.5039467311620314 2202 14-15 0.6479971762751404 2202 16-17 0.5626098489092115 2202 18-19 0.5177681604900854 2202 20-21 0.5844775435101752 2202 22-23 0.5183199626192874 2202 24-25 0.3957858470558975 2202 26-27 0.5471825414952036 2202 28-29 0.38557659810678757 2202 30-31 0.3913931064024112 2202 32-33 0.420287994148147 2202 34-35 0.6296772946076814 2202 36-37 0.7049244292449757 2202 38-39 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0.5306885179314627 2204 84-85 0.5554196414970995 2204 86-87 0.27850738400332276 2204 88-89 0.025398743612178976 2204 90-91 0.2093695414858665 2204 92-93 0.3359703294125538 2204 94-95 0.40160046971266183 2204 96-97 0.6519825754903437 2204 98-99 0.5781368734781047 2204 100-101 0.20896485167823897 2205 1 0.45147686003429044 2205 2 0.31104828850191524 2205 3 0.2763904977724465 2205 4 0.28533567566555007 2205 5 0.23892904892853295 2205 6 0.15614550043558495 2205 7 0.20525300150347192 2205 8 0.25242033618397386 2205 9 0.2543121019651977 2205 10-11 0.3162483725162133 2205 12-13 0.3799731818570393 2205 14-15 0.46733106655773327 2205 16-17 0.43089454507022396 2205 18-19 0.45420223092219203 2205 20-21 0.35239031422886313 2205 22-23 0.27213840115570065 2205 24-25 0.33330584868598834 2205 26-27 0.45901715393403464 2205 28-29 0.23297929279830498 2205 30-31 0.40868050780262166 2205 32-33 0.3818027602953791 2205 34-35 0.41987382897691816 2205 36-37 0.42326971461355356 2205 38-39 0.521788060954421 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1216.0 9 1552.0 10 1834.0 11 2635.0 12 3911.0 13 6240.0 14 5737.0 15 6536.0 16 8688.0 17 12368.0 18 11078.0 19 5977.0 20 5930.0 21 6091.0 22 6435.0 23 7261.0 24 7954.0 25 9019.0 26 10091.0 27 11877.0 28 13686.0 29 16409.0 30 19540.0 31 23656.0 32 29283.0 33 38049.0 34 51604.0 35 77368.0 36 118572.0 37 198236.0 38 219791.0 39 37960.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.740742627209286 21.925431671165892 15.549432078643102 18.784393622981714 2 13.82253652556561 25.16500417275299 37.57490371156468 23.437555590116727 3 23.03894712554418 26.61347948458104 32.420633428235426 17.926939961639363 4 11.088962371652233 18.466932248237107 43.3112783391766 27.132827040934053 5 10.09708402256652 41.4882963375665 36.179226723574374 12.235392916292609 6 24.87772408782119 40.795288833335874 17.485277604174605 16.841709474668335 7 22.9462113374297 36.37609989003641 23.651624757866667 17.026064014667234 8 22.814313867181347 30.523532060253295 21.47811849218376 25.184035580381597 9 21.96441564670924 21.734340892738825 26.755031360896236 29.5462120996557 10-11 23.357810732726513 26.482388365478126 28.562391454792856 21.597409447002498 12-13 27.544487855492534 26.201811068953447 27.416795350021268 18.83690572553276 14-15 22.439835180330583 28.34927785955978 30.79006833949112 18.420818620618515 16-17 17.74232228615095 31.13841122995848 30.93842789523637 20.180838588654197 18-19 17.169673255754866 33.2223690228162 31.467808323593676 18.140149397835255 20-21 20.177018044843013 28.520934752039047 29.60021019368209 21.701837009435845 22-23 18.508112778420134 28.673468609836984 31.18305528378842 21.63536332795446 24-25 21.949363575505547 29.05873424696249 30.059893692888647 18.932008484643315 26-27 20.400869608091806 26.54993613148425 35.018194775336845 18.030999485087097 28-29 17.389644682435403 32.32045420467166 31.875093715293573 18.414807397599365 30-31 18.91517309202351 28.581644088341353 31.07453515586287 21.42864766377227 32-33 19.444066115232076 29.318051995308263 32.12430045799292 19.113581431466738 34-35 21.83020437183685 27.86947949787897 31.05212253448173 19.248193595802448 36-37 20.29118576942029 26.896936234376895 34.08501743013409 18.726860566068726 38-39 19.079971012911088 29.922590601227107 32.473291233037536 18.524147152824277 40-41 18.634457187783934 30.460881278915974 32.00507218223145 18.899589351068634 42-43 17.949798157276668 30.48633513287448 29.227744385253114 22.336122324595742 44-45 19.980974774999936 27.578466301633974 30.217259908546307 22.223299014819784 46-47 22.164313977742083 30.00462665165598 30.07180747007852 17.759251900523417 48-49 20.414470448752876 28.914624515607684 30.859744065365156 19.81116097027428 50-51 16.512208708001 30.048194147736893 33.86852913991198 19.571068004350124 52-53 19.225750503736577 32.07212997678986 28.677787129849264 20.024332389624302 54-55 17.871662821757205 28.562109271595805 30.646776167103862 22.919451739543128 56-57 20.904868094888663 28.156245330809444 29.43269047875235 21.50619609554955 58-59 23.184422340880598 27.582667855629705 29.30528767348179 19.927622130007908 60-61 20.15369153238959 29.153126834757646 32.48596410398591 18.207217528866853 62-63 18.570320432758386 29.279988902584993 32.05638262010172 20.0933080445549 64-65 18.092787423330567 31.94988677181575 30.175272841917746 19.782052962935946 66-67 19.71177368821417 28.778907960329725 28.14612024038076 23.36319811107534 68-69 18.11986841507726 28.82719095172515 30.351735050380167 22.701205582817423 70-71 21.587390348471576 28.816368097495914 28.991524822713128 20.60471673131938 72-73 20.31409109182443 26.095640497680765 33.10282164670543 20.48744676378938 74-75 17.932386010258274 32.039220828386426 29.50965290954648 20.518740251808822 76-77 18.98582313115325 28.05385710503778 29.744746988824488 23.21557277498448 78-79 19.28501815299926 28.404968704627066 30.872695430972446 21.437317711401228 80-81 21.099293854607286 27.62529924224265 29.77765039122066 21.4977565119294 82-83 19.401432050051362 27.111325110453926 32.43254685174436 21.054695987750353 84-85 18.766245424757923 28.544358935646695 31.10139389314846 21.58800174644692 86-87 18.709369731932682 29.212171291726918 30.479134309097354 21.599324667243046 88-89 18.734533278034462 28.605585900216145 28.803008993208547 23.85687182854085 90-91 19.459446221414492 26.02353219694554 30.4361890310635 24.080832550576467 92-93 21.212498431216897 28.11371560283615 30.588464572030865 20.085321393916082 94-95 19.841501051268537 26.786301927589257 31.045763471976972 22.326433549165234 96-97 17.254348944090253 27.166002726508353 32.94416984351148 22.635478485889912 98-99 19.289674692722265 28.40316593507731 29.11572580398303 23.19143356821739 100-101 18.418723611769288 25.606116330874812 31.317068890848816 24.65809116650709 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2693.0 1 1579.5 2 576.5 3 632.0 4 492.0 5 494.5 6 873.5 7 1622.5 8 2363.5 9 2529.0 10 2070.0 11 1665.0 12 1620.0 13 1659.0 14 1656.0 15 1761.5 16 1928.0 17 1873.5 18 1812.5 19 1939.5 20 2184.0 21 2496.0 22 2888.0 23 3345.5 24 3843.5 25 5021.0 26 7679.0 27 10312.0 28 11076.5 29 12256.0 30 15290.0 31 18300.0 32 22074.5 33 27024.5 34 32760.0 35 36429.0 36 39202.5 37 42836.5 38 40856.5 39 36574.5 40 33789.0 41 31245.0 42 28956.5 43 27902.5 44 27409.0 45 26674.5 46 30545.0 47 53755.5 48 103709.5 49 99710.0 50 45464.0 51 19454.0 52 11338.0 53 8378.5 54 6538.0 55 5081.0 56 4074.0 57 3444.5 58 2900.0 59 2392.0 60 1963.0 61 1620.5 62 1350.0 63 1058.0 64 750.5 65 533.0 66 395.0 67 306.0 68 245.5 69 194.0 70 146.5 71 121.0 72 105.5 73 89.5 74 65.0 75 52.0 76 42.5 77 34.0 78 27.5 79 20.0 80 21.0 81 17.0 82 9.0 83 5.0 84 3.5 85 2.5 86 2.0 87 1.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 1.5 95 1.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32917314791875657 2 0.11463185811236155 3 0.10874288754503032 4 0.1156471978653497 5 0.13311104161674578 6 0.08599927707809588 7 0.09442659702789748 8 0.09940176181753939 9 0.08772535465817573 10-11 0.0868115488804864 12-13 0.09011140307769785 14-15 0.09214208258367415 16-17 0.08224251999203974 18-19 0.09564500473148324 20-21 0.10534149937252003 22-23 0.1121442757175406 24-25 0.12514062455578887 26-27 0.12493755660519122 28-29 0.1212823334944339 30-31 0.11112893596455246 32-33 0.10498613045897419 34-35 0.32252267253668426 36-37 0.4764989460773364 38-39 0.45238462694386794 40-41 0.43141786104466273 42-43 0.4619288206219565 44-45 0.4685285290163795 46-47 0.4773619848673763 48-49 0.4871600134837119 50-51 0.47619434415144 52-53 0.47893576148450795 54-55 0.4661932475845067 56-57 0.4462925884259391 58-59 0.4270011331191643 60-61 0.38948432924625237 62-63 0.4552275782522348 64-65 0.48650004264426966 66-67 0.4938104888657843 68-69 0.5069083716793313 70-71 0.49878565365542615 72-73 0.4900029647920788 74-75 0.49777031390243803 76-77 0.4740113636825154 78-79 0.45441530644984424 80-81 0.45680135486936635 82-83 0.4689854319052241 84-85 0.4686808299793277 86-87 0.4705592085223557 88-89 0.48365709133590284 90-91 0.49909025558132264 92-93 0.4907137026191704 94-95 0.4960442363223582 96-97 0.49589193535940995 98-99 0.4908152365944693 100-101 0.47685431499088227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 984892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.063385166910567 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64281130161986 18.206747592597026 2 12.283302718975854 6.157222943349034 3 4.8649393245058565 3.6579554431122006 4 2.5453879281417624 2.551841521688859 5 1.5064099451670567 1.887786633749329 6 0.9853856336487652 1.4818259804447542 7 0.7215598157576226 1.265931210830883 8 0.48452361841383523 0.9715041656616923 9 0.38303599771783586 0.8640160869234599 >10 2.8057079003201526 14.32532202042503 >50 0.4349217728189132 7.557520323772486 >100 0.3014429820687763 13.950647844847477 >500 0.022314083464041314 3.5841862694353575 >1k 0.012171318253113444 7.060790007292679 >5k 0.004462816692808263 8.374049360156889 >10k+ 0.0016228424337484592 8.102652595712865 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32900 3.3404677873309967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20881 2.1201309382145452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14671 1.4896049516089074 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11232 1.1404296105562843 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9077 0.9216238937873391 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9055 0.9193901463307652 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8432 0.8561344797196039 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8414 0.8543068681642251 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8285 0.841208985350678 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7725 0.7843499591833419 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7703 0.7821162117267679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6698 0.6800745665514595 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5884 0.5974259106582245 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5596 0.5681841257721659 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5484 0.5568123205386987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4997 0.507365274568176 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4920 0.4995471584701673 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4625 0.469594635757017 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4467 0.45355226765980433 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3736 0.37933093171637094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.3621716898908713 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3440 0.3492768750279218 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3114 0.31617679908050833 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3099 0.3146537894510261 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2899 0.29434699439126316 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2800 0.28429513083668057 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2783 0.28256905325660076 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2320 0.23555882269324963 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2063 0.2094645910414543 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1921 0.19504676654902264 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1597 0.16214975855220673 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1543 0.15666692388607076 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1507 0.15301170077531345 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1321 0.13412638136973395 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1284 0.1303696242836778 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1272 0.12915121658009204 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1205 0.12234844023507145 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.12021622675379634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.11981009085260111 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1173 0.11909935302550939 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1143 0.11605333376654497 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1087 0.11036743114981136 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1074 0.10904748947092675 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1066 0.10823521766853625 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1051 0.10671220803905404 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.0766987649407247E-5 0.0 12-13 0.0 0.0 0.0 1.0153397529881449E-4 0.0 14-15 0.0 0.0 0.0 2.0306795059762899E-4 0.0 16-17 0.0 0.0 0.0 6.599708394422943E-4 0.0 18-19 0.0 0.0 0.0 8.630387900399232E-4 0.0 20-21 0.0 0.0 0.0 0.0013199416788845883 0.0 22-23 0.0 0.0 0.0 0.0025383493824703623 0.0 24-25 0.0 0.0 0.0 0.004873630814343095 0.0 26-27 0.0 0.0 0.0 0.010356465480479079 0.0 28-29 0.0 0.0 0.0 0.024215853108767255 0.0 30-31 0.0 0.0 0.0 0.060818851203989874 0.0 32-33 0.0 0.0 0.0 0.13011578934543078 0.0 34-35 0.0 0.0 0.0 0.2256084931139658 0.0 36-37 0.0 0.0 0.0 0.3507491176697547 0.0 38-39 0.0 0.0 0.0 0.5232553417024405 0.0 40-41 0.0 0.0 0.0 0.7071841379562429 0.0 42-43 0.0 0.0 0.0 0.8989818172957035 0.0 44-45 0.0 0.0 0.0 1.138906600926802 0.0 46-47 0.0 0.0 0.0 1.4240647705535225 0.0 48-49 0.0 0.0 0.0 1.678305844701754 0.0 50-51 0.0 0.0 0.0 1.9422941804786718 0.0 52-53 0.0 0.0 0.0 2.196738322577501 0.0 54-55 0.0 0.0 0.0 2.4852471133890823 0.0 56-57 0.0 0.0 0.0 2.902704052830158 0.0 58-59 0.0 0.0 0.0 3.2773644216827833 0.0 60-61 0.0 0.0 0.0 3.696242836778043 0.0 62-63 0.0 0.0 0.0 4.109536883231867 0.0 64-65 0.0 0.0 0.0 4.541716249091271 0.0 66-67 0.0 0.0 0.0 5.059590290102874 0.0 68-69 0.0 0.0 0.0 5.647015104194166 0.0 70-71 0.0 0.0 0.0 6.270586013491835 0.0 72-73 0.0 0.0 0.0 6.843542236103045 0.0 74-75 0.0 0.0 0.0 7.520012346531397 0.0 76-77 0.0 0.0 0.0 8.170388225307953 0.0 78-79 0.0 0.0 0.0 9.077340459664612 0.0 80-81 0.0 0.0 0.0 9.999776625254343 0.0 82-83 0.0 0.0 0.0 10.796919865325336 0.0 84-85 0.0 0.0 0.0 11.677320965141355 0.0 86-87 0.0 0.0 0.0 12.574119801968134 0.0 88-89 0.0 0.0 0.0 13.56798511918058 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCCGT 55 5.0364113E-5 43.07419 6 GCCCGTG 50 0.0016356327 37.905285 7 CATTCCG 295 0.0 32.118225 9 TCCGCGC 30 0.003934942 31.69405 76-77 CATATAG 335 0.0 29.707943 3 CCCGTGC 65 0.005918301 29.15495 8 GTAAGCG 150 0.0 28.52901 94-95 GACATAT 355 0.0 28.091373 1 TATCGAG 35 0.0083493665 27.171871 66-67 GCCGGAT 35 0.008351444 27.170485 92-93 CCCTATA 160 6.380105E-8 26.657576 2 AAACGTA 45 8.898133E-4 26.415075 84-85 GTGTAGC 245 1.0913936E-11 25.197609 1 TAGGCAT 385 0.0 24.618826 5 CATGGGG 1040 0.0 24.151306 4 GGATATG 455 0.0 23.94749 9 GAGTACT 4600 0.0 23.895597 12-13 CCTATAC 260 2.5465852E-11 23.695621 3 ACTATAT 240 2.3646862E-10 23.688395 8 GGCATTC 385 0.0 23.38313 7 >>END_MODULE