##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 984892 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.391851086210465 33.0 31.0 34.0 31.0 34.0 2 32.58420212571531 34.0 31.0 34.0 31.0 34.0 3 32.68315307668252 34.0 31.0 34.0 31.0 34.0 4 36.12640878390727 37.0 35.0 37.0 35.0 37.0 5 36.10549176965596 37.0 35.0 37.0 35.0 37.0 6 36.18954971712635 37.0 36.0 37.0 35.0 37.0 7 36.15442099235246 37.0 36.0 37.0 35.0 37.0 8 36.16508409043834 37.0 37.0 37.0 35.0 37.0 9 37.84757415026216 39.0 38.0 39.0 35.0 39.0 10-11 37.78757772425809 39.0 38.0 39.0 35.0 39.0 12-13 37.773365506065645 39.0 38.0 39.0 35.0 39.0 14-15 38.96937329169087 40.0 38.0 41.0 36.0 41.0 16-17 38.97142732401116 40.0 38.0 41.0 35.5 41.0 18-19 39.09558713036556 40.5 38.5 41.0 36.0 41.0 20-21 39.177583938137374 41.0 39.0 41.0 36.0 41.0 22-23 39.136092079131515 40.5 39.0 41.0 35.5 41.0 24-25 39.0346748679043 40.5 38.5 41.0 35.0 41.0 26-27 38.839982962598945 40.0 38.0 41.0 35.0 41.0 28-29 38.737633669478484 40.0 38.0 41.0 35.0 41.0 30-31 38.61449631025533 40.0 38.0 41.0 35.0 41.0 32-33 38.41172534653546 40.0 38.0 41.0 34.0 41.0 34-35 38.25813185608168 40.0 38.0 41.0 34.0 41.0 36-37 38.10046380719916 40.0 37.5 41.0 33.5 41.0 38-39 37.9378530843991 40.0 37.0 41.0 33.0 41.0 40-41 37.90382448024758 40.0 38.0 41.0 33.0 41.0 42-43 37.68868007862791 40.0 37.0 41.0 33.0 41.0 44-45 37.420241508713644 40.0 37.0 41.0 33.0 41.0 46-47 37.07017317634827 40.0 37.0 41.0 31.5 41.0 48-49 36.680982280290635 40.0 37.0 41.0 31.0 41.0 50-51 36.426405128684166 40.0 37.0 41.0 30.0 41.0 52-53 36.320577789239834 40.0 37.0 41.0 30.0 41.0 54-55 36.16469013861418 40.0 36.0 41.0 29.5 41.0 56-57 35.85588166012111 39.5 35.5 41.0 28.5 41.0 58-59 35.60001350401872 39.0 35.0 40.5 28.0 41.0 60-61 35.1124280631785 39.0 35.0 40.0 26.5 41.0 62-63 34.83736034001698 38.0 35.0 40.0 26.0 41.0 64-65 34.45852540176994 38.0 34.0 40.0 26.0 41.0 66-67 33.97537496497078 37.0 34.0 39.5 25.0 41.0 68-69 33.44515388489296 36.5 33.5 39.0 23.5 40.0 70-71 32.911588783338686 36.0 33.0 38.5 22.0 40.0 72-73 32.39883103934238 35.5 32.5 37.5 21.0 40.0 74-75 31.928571356047158 35.0 32.0 37.0 19.5 39.0 76-77 29.925612148337077 33.5 29.5 35.5 13.0 37.5 78-79 30.866648830531673 34.5 31.0 36.0 13.5 38.0 80-81 30.825061021919154 35.0 31.0 36.0 12.5 37.0 82-83 30.49761801293949 34.5 31.0 35.0 7.5 37.0 84-85 30.111362971777616 34.0 31.0 35.0 4.0 36.5 86-87 29.717510650914008 34.0 30.5 35.0 2.0 36.0 88-89 29.147714165614097 34.0 29.5 35.0 2.0 36.0 90-91 28.675260333112668 34.0 29.0 35.0 2.0 35.0 92-93 28.480785202844576 34.0 29.0 35.0 2.0 35.0 94-95 28.17676557429647 34.0 29.0 35.0 2.0 35.0 96-97 27.60448861398001 34.0 29.0 35.0 2.0 35.0 98-99 26.977030984107902 34.0 27.0 35.0 2.0 35.0 100-101 23.48739658764616 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05416501124231132 1101 2 0.13696984760069597 1101 3 0.2056372524765422 1101 4 0.25508248555888 1101 5 0.08880093277921475 1101 6 0.21550416293131747 1101 7 0.1406340268909716 1101 8 0.18877142869558838 1101 9 0.29354707747908293 1101 10-11 0.11760405609994251 1101 12-13 0.19948642612212808 1101 14-15 0.1982004183335846 1101 16-17 0.3271712386847767 1101 18-19 0.3030802427440378 1101 20-21 0.1807565301272973 1101 22-23 0.2841724972327384 1101 24-25 0.2796130261159533 1101 26-27 0.32621300903822714 1101 28-29 0.2782120938102679 1101 30-31 0.2988114405091764 1101 32-33 0.30295180157927604 1101 34-35 0.4916572191939963 1101 36-37 0.4542333586734131 1101 38-39 0.3023497812288838 1101 40-41 0.27542498617209077 1101 42-43 0.44156777198517005 1101 44-45 0.4779029806159443 1101 46-47 0.17309079342622624 1101 48-49 0.05066915938667904 1101 50-51 0.1742894713239025 1101 52-53 0.15977779700406813 1101 54-55 0.18607456509744935 1101 56-57 0.09350665485415277 1101 58-59 0.34494515122816694 1101 60-61 0.39797430760901875 1101 62-63 0.17851503534887314 1101 64-65 0.2211605282320761 1101 66-67 0.24405214279106957 1101 68-69 -0.03206984076873454 1101 70-71 0.13531225334297403 1101 72-73 0.11852008872943998 1101 74-75 0.18838760527519227 1101 76-77 0.16476843351125936 1101 78-79 0.410871673075178 1101 80-81 0.38672013153720997 1101 82-83 0.48133365191140953 1101 84-85 0.23450807539853002 1101 86-87 0.3799050297154345 1101 88-89 0.5022333367898426 1101 90-91 0.6117462190963217 1101 92-93 0.6286319383116208 1101 94-95 0.5391960262508668 1101 96-97 0.654224532115883 1101 98-99 0.6164370038032878 1101 100-101 0.17280002042981124 1102 1 0.14343983835398433 1102 2 0.029111340217738757 1102 3 0.12723798641723505 1102 4 0.07488378737457424 1102 5 0.10406910565011884 1102 6 0.11191342777126323 1102 7 0.10251440152588742 1102 8 0.071143497142522 1102 9 0.15342188949387747 1102 10-11 0.233764396592683 1102 12-13 0.08864773629916556 1102 14-15 0.14136501498555276 1102 16-17 0.16754929943230223 1102 18-19 0.22374903458707962 1102 20-21 0.19846488160592202 1102 22-23 0.19902608651627673 1102 24-25 0.13589797394461556 1102 26-27 0.00615912478485825 1102 28-29 0.1763066931913002 1102 30-31 0.18218222572119913 1102 32-33 0.12995025124569537 1102 34-35 0.29839971865689563 1102 36-37 0.47580738274712786 1102 38-39 0.5327281903659795 1102 40-41 0.5427472058781717 1102 42-43 0.4526218826917656 1102 44-45 0.6706480581616603 1102 46-47 0.7287445754853294 1102 48-49 0.6981874643552928 1102 50-51 0.7409944608431829 1102 52-53 0.8380922882682 1102 54-55 0.7462719277884062 1102 56-57 0.9057740322355343 1102 58-59 0.8932435585071943 1102 60-61 1.001274718128066 1102 62-63 0.9896167592965952 1102 64-65 0.9769699242987571 1102 66-67 0.815736199903732 1102 68-69 0.8870326496360335 1102 70-71 0.9708593120640359 1102 72-73 0.7063026471863054 1102 74-75 0.835367358034727 1102 76-77 0.6697939530472006 1102 78-79 0.7804563927086754 1102 80-81 0.7737678783276714 1102 82-83 0.8005267236041753 1102 84-85 0.9357756327811977 1102 86-87 0.9644432103089748 1102 88-89 1.001482847592822 1102 90-91 1.1409325552574145 1102 92-93 1.1693702919386482 1102 94-95 1.1353312246296952 1102 96-97 1.1096785708219628 1102 98-99 0.9195388322678788 1102 100-101 0.8882150975690664 1103 1 0.20263106598472547 1103 2 0.12939521764948836 1103 3 0.1474879098816544 1103 4 0.19852910757161624 1103 5 0.152879325237663 1103 6 0.11728205096571287 1103 7 0.22533592054845997 1103 8 0.26833812016999303 1103 9 0.21118538062966508 1103 10-11 0.17505464735108234 1103 12-13 0.20033402390595967 1103 14-15 0.19476041171817826 1103 16-17 0.19736060443356251 1103 18-19 0.22075162288130912 1103 20-21 0.271478435361729 1103 22-23 0.312321012902963 1103 24-25 0.31057722027786383 1103 26-27 0.15913288003798698 1103 28-29 0.3977895353053498 1103 30-31 0.48675487886193736 1103 32-33 0.49610899995850133 1103 34-35 0.3092872530979065 1103 36-37 0.45803721214236504 1103 38-39 0.39164110678912323 1103 40-41 0.5879115486462538 1103 42-43 0.4819837150927526 1103 44-45 0.8213702757453589 1103 46-47 0.8453722785829285 1103 48-49 0.8885133667793852 1103 50-51 0.9197579241478522 1103 52-53 0.9841514015686883 1103 54-55 0.9326221546120408 1103 56-57 1.008221779306318 1103 58-59 1.010433450244129 1103 60-61 0.9020345077247853 1103 62-63 0.8387275549883384 1103 64-65 0.8250760165310922 1103 66-67 0.8987077286671976 1103 68-69 0.9023508985019149 1103 70-71 0.7537278656806876 1103 72-73 0.8716375947246391 1103 74-75 0.752383305266914 1103 76-77 0.9277800931490638 1103 78-79 1.0286918996525394 1103 80-81 0.9552184363416387 1103 82-83 0.6311145168746144 1103 84-85 0.5533454193164999 1103 86-87 0.9598148949267156 1103 88-89 0.9551523808649272 1103 90-91 1.1060156311528857 1103 92-93 1.1482589952867315 1103 94-95 0.8750184028323602 1103 96-97 0.7718421621497882 1103 98-99 0.8455985021907857 1103 100-101 0.4357910069509394 1104 1 0.12586495465836123 1104 2 0.11970904949760808 1104 3 0.028451360740497478 1104 4 0.001047511291105252 1104 5 0.12331714003812522 1104 6 0.18624756820709365 1104 7 0.16356399752120865 1104 8 0.1394733390773908 1104 9 0.32411318638970954 1104 10-11 0.24890845078643764 1104 12-13 0.2128227233764548 1104 14-15 0.19215806120804757 1104 16-17 0.09732185976094598 1104 18-19 0.10048815910757725 1104 20-21 0.2078467680493148 1104 22-23 0.18902254977497535 1104 24-25 0.1615910392110962 1104 26-27 0.1537990301090133 1104 28-29 0.18575931446071792 1104 30-31 0.2728003392778007 1104 32-33 0.23305850273521145 1104 34-35 0.19830313841367797 1104 36-37 0.1882322269944794 1104 38-39 0.27926218389102075 1104 40-41 0.17075411612655955 1104 42-43 0.24278702130481378 1104 44-45 0.40773215098751336 1104 46-47 0.5362479081838671 1104 48-49 0.24173563205123827 1104 50-51 0.3645273933458384 1104 52-53 0.4850425290386582 1104 54-55 0.40398338410965096 1104 56-57 0.5912257829224856 1104 58-59 0.6401570715795302 1104 60-61 0.5763080451134002 1104 62-63 0.593919341117747 1104 64-65 0.33678982254943435 1104 66-67 0.5643024593917261 1104 68-69 0.6339407215679103 1104 70-71 0.45201664264052255 1104 72-73 0.3690158718781973 1104 74-75 0.580705660943007 1104 76-77 0.4854902829979615 1104 78-79 0.7272325169843228 1104 80-81 0.5525515113771569 1104 82-83 0.727879336711883 1104 84-85 0.57863277563834 1104 86-87 0.5615906924212268 1104 88-89 0.6363224699776744 1104 90-91 0.6912345385531182 1104 92-93 0.6735205218268305 1104 94-95 0.7680120745358323 1104 96-97 0.7964321143646913 1104 98-99 0.8197687204524406 1104 100-101 0.5441146541161217 1105 1 0.00656087474737177 1105 2 0.08932917885169189 1105 3 0.040296184743134233 1105 4 0.07713779017264244 1105 5 -0.03646816641368389 1105 6 -0.07664688228256722 1105 7 -0.018998876144600274 1105 8 0.05148321791492094 1105 9 0.08968274783289587 1105 10-11 0.08861636486011548 1105 12-13 0.1777895290152216 1105 14-15 0.11549009040440694 1105 16-17 0.15559294093124976 1105 18-19 0.11929951977072761 1105 20-21 0.04340569262537031 1105 22-23 0.07309109032344452 1105 24-25 0.021007723198330552 1105 26-27 0.07825098692578081 1105 28-29 -0.006298491410348106 1105 30-31 -0.0030741456899860964 1105 32-33 -0.03363159764258228 1105 34-35 0.11878033028075663 1105 36-37 0.11830949710977734 1105 38-39 0.2279661977800771 1105 40-41 0.04668545331524854 1105 42-43 0.034792865468595835 1105 44-45 -0.06441637391131394 1105 46-47 -0.031744902761104754 1105 48-49 -0.04342227040746849 1105 50-51 0.031014537408140086 1105 52-53 0.14774563818270536 1105 54-55 0.1433709695408112 1105 56-57 0.020833563729659943 1105 58-59 -9.51363333847155E-4 1105 60-61 0.1150959313111457 1105 62-63 0.237825149825305 1105 64-65 -0.036303794107659826 1105 66-67 0.005758120772561881 1105 68-69 -0.016385108902433387 1105 70-71 -0.016518404002752618 1105 72-73 -0.10226519816617952 1105 74-75 -0.14994868978017095 1105 76-77 -0.09010666532153877 1105 78-79 0.043618794203087674 1105 80-81 -0.18008105210782333 1105 82-83 -0.3087976815419573 1105 84-85 -0.28562184895360687 1105 86-87 -5.255550876697157E-4 1105 88-89 0.05993081319391891 1105 90-91 0.151439817862574 1105 92-93 0.2227233681057612 1105 94-95 0.38132710919735047 1105 96-97 0.1953115754300896 1105 98-99 0.36565090797918387 1105 100-101 0.22934346902240677 1106 1 -0.019424679305572568 1106 2 0.032013557042091634 1106 3 0.039445605155421504 1106 4 -0.02225602701764018 1106 5 0.0034430210255465 1106 6 0.07591742976194382 1106 7 0.11828513284326903 1106 8 0.06388731043109175 1106 9 0.08641948881518857 1106 10-11 0.12478106209897533 1106 12-13 0.03515463277086184 1106 14-15 0.028982438085193962 1106 16-17 0.005221131039270688 1106 18-19 -0.080919266913412 1106 20-21 0.144501827818857 1106 22-23 0.10083226845728888 1106 24-25 0.16711388804621663 1106 26-27 0.15851777225640973 1106 28-29 0.07397292923138821 1106 30-31 0.2097874520322236 1106 32-33 0.30394014828210913 1106 34-35 0.020840262535685383 1106 36-37 0.22125743908959095 1106 38-39 0.2585905394210499 1106 40-41 0.11330356509809292 1106 42-43 0.19406736975104621 1106 44-45 0.3057410296655263 1106 46-47 0.4537946648338931 1106 48-49 0.5098328188744219 1106 50-51 0.4944007944060118 1106 52-53 0.5359471490623591 1106 54-55 0.5515674742844752 1106 56-57 0.41286905630679627 1106 58-59 0.4380068041422618 1106 60-61 0.43548168190127967 1106 62-63 0.43818589291203125 1106 64-65 0.4409623225793453 1106 66-67 0.5044144599660854 1106 68-69 0.4133163422975912 1106 70-71 0.5728127321931211 1106 72-73 0.36913493231782724 1106 74-75 0.36629575798624714 1106 76-77 0.4607131473887165 1106 78-79 0.6164650258689832 1106 80-81 0.614735163601015 1106 82-83 0.6485174767365329 1106 84-85 0.5964709013039418 1106 86-87 0.4641041241140371 1106 88-89 0.49672081390080436 1106 90-91 0.6199556260116843 1106 92-93 0.6657409092818085 1106 94-95 0.5486734283547428 1106 96-97 0.7532622348610332 1106 98-99 0.6943095300634319 1106 100-101 0.15856284786754316 1107 1 -0.06388461685182278 1107 2 -0.08363079053286526 1107 3 0.01434452394750707 1107 4 -0.0588934523348712 1107 5 -0.03742605173486879 1107 6 0.04154417837658997 1107 7 0.10508704075106579 1107 8 0.06796553104082648 1107 9 0.07503205898539989 1107 10-11 0.030828561241598607 1107 12-13 -0.04444851067420075 1107 14-15 -0.04000605038974925 1107 16-17 0.0666110427737081 1107 18-19 0.11267155274688179 1107 20-21 0.1389158501289245 1107 22-23 0.11738262404654165 1107 24-25 -0.0862569897357659 1107 26-27 0.15726384633450152 1107 28-29 0.058419283990325255 1107 30-31 0.174993969062605 1107 32-33 0.1801923819962994 1107 34-35 0.028650929310650497 1107 36-37 0.053516220519526314 1107 38-39 -0.022356555396733313 1107 40-41 0.10396088626557543 1107 42-43 0.07300626525834986 1107 44-45 0.10504575791952675 1107 46-47 -0.009602882141791724 1107 48-49 0.31516688324148845 1107 50-51 0.1434611678649773 1107 52-53 0.025289624829945012 1107 54-55 0.13648076556080468 1107 56-57 0.35084061819195966 1107 58-59 0.43970239627959273 1107 60-61 0.23114759948399666 1107 62-63 0.13141458011983786 1107 64-65 0.10545055383871471 1107 66-67 0.21184697472214253 1107 68-69 0.3073564995149738 1107 70-71 0.1643369149696099 1107 72-73 -0.03877907194202379 1107 74-75 0.16368152268362834 1107 76-77 0.3916462532893341 1107 78-79 0.29574089634063583 1107 80-81 0.19204874999837074 1107 82-83 0.1703057792942495 1107 84-85 0.6019378603841012 1107 86-87 0.4606599173307657 1107 88-89 0.5696577870601338 1107 90-91 0.6348036206327201 1107 92-93 0.18404946869409144 1107 94-95 0.19904187838272236 1107 96-97 0.49353147639097017 1107 98-99 0.7851562656819233 1107 100-101 0.8416805939371059 1108 1 -0.20396656399285717 1108 2 -0.05674762424226287 1108 3 0.05990739673265466 1108 4 0.02365276865129573 1108 5 0.02874495279539957 1108 6 -0.11468122436256323 1108 7 0.027723735928695703 1108 8 -0.04952957137123093 1108 9 -0.1297811330175378 1108 10-11 -0.051325196782578075 1108 12-13 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-1.0710958614707558 2316 100-101 -0.9977223568490885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 18.0 6 62.0 7 119.0 8 231.0 9 468.0 10 907.0 11 1565.0 12 2303.0 13 3390.0 14 3995.0 15 5715.0 16 7860.0 17 11591.0 18 10921.0 19 6270.0 20 5245.0 21 5207.0 22 5492.0 23 5940.0 24 7020.0 25 7780.0 26 8957.0 27 10393.0 28 12567.0 29 15638.0 30 18885.0 31 23173.0 32 29114.0 33 37443.0 34 51431.0 35 75765.0 36 120975.0 37 229979.0 38 234971.0 39 23496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.333024306804255 22.367985394930813 15.76992940951143 18.529060888753506 2 13.959500127932808 25.538231603059014 37.39476003460278 23.107508234405397 3 23.017041462414152 26.59144352883362 32.70886554058719 17.68264946816504 4 11.196760660052067 18.914358122514958 42.90165825288458 26.987222964548398 5 9.909614455188995 41.80793427096575 36.09471901487676 12.187732258968497 6 24.708089821015907 41.32737396587646 17.09385394540721 16.870682267700417 7 22.962213115752796 36.52948749710628 23.44378876059507 17.064510626545854 8 22.827680598481866 30.887041421800564 21.119879133955806 25.165398845761764 9 21.73517502426662 21.945451887110465 26.294050515183393 30.02532257343952 10-11 23.364643026849645 26.720493211438413 28.40098203660909 21.513881725102856 12-13 27.72588263484727 26.175560366009677 27.23191984501854 18.866637154124515 14-15 22.267484156771683 28.64711547394644 30.572874281197436 18.512526088084442 16-17 17.700069297958375 31.358468438968295 30.763369048434956 20.178093214638366 18-19 17.1803608923618 33.41879109587651 31.196872347424897 18.2039756643368 20-21 20.15302185417284 28.470329741738183 29.529278337117166 21.84737006697181 22-23 18.53233654045317 28.77620084232586 31.09229235286712 21.59917026435386 24-25 22.060946047137364 29.179830183650417 30.09435087763831 18.66487289157391 26-27 20.597554121993355 26.56832319421169 35.04726203101591 17.78686065277904 28-29 17.344033660543488 32.580881964723034 31.784601763442083 18.290482611291388 30-31 19.02528822463575 28.6225377983085 31.034136133309577 21.318037843746175 32-33 19.53833516771382 29.437846992360583 32.04148272094808 18.98233511897751 34-35 21.924098423163958 27.844252852262187 31.214265804281833 19.01738292029202 36-37 20.35457009696038 26.896120264586965 34.153879278388104 18.59543036006455 38-39 19.020370608274682 29.934795976554785 32.582360638523134 18.462472776647402 40-41 18.60508805467336 30.396417392099067 32.06677786155475 18.931716691672822 42-43 17.921989697327255 30.577085742892162 29.305854123658477 22.19507043612211 44-45 20.023008241235214 27.503377368155725 30.231468389814033 22.24214600079503 46-47 22.243567223554592 29.992598067984055 30.02737394885458 17.73646075960677 48-49 20.560207252518186 28.87543780469912 30.900038633932574 19.664316308850115 50-51 16.51041410187224 30.048061180405334 33.8255369505544 19.615987767168026 52-53 19.20886716963239 32.21969120456416 28.650617859936855 19.920823765866597 54-55 17.926026406956296 28.637860800981223 30.436027503523228 23.000085288539253 56-57 20.943565385849414 28.244518180673616 29.31321403768129 21.498702395795682 58-59 23.292106942553993 27.746202073114944 29.11321713586838 19.84847384846268 60-61 20.05454934090444 29.632162583843463 32.15527268831683 18.15801538693527 62-63 18.420635790961484 29.560634491273817 31.783608095429567 20.235121622335132 64-65 17.954243846668554 32.51739056710865 29.670729119389 19.857636466833796 66-67 19.699253540769426 29.038193145247064 27.845422067026487 23.417131246957023 68-69 18.123526437313807 29.22091306563992 29.826837980703818 22.828722516342456 70-71 21.67334083330964 29.26265011798248 28.508354215487586 20.55565483322029 72-73 20.497932768262917 26.512043960149946 32.56793638287244 20.422086888714702 74-75 17.900439845180994 32.56524573252702 28.818540510025464 20.71577391226652 76-77 19.126170965400018 28.479496372315737 29.1346214016328 23.259711260651443 78-79 19.374508510853246 28.979951558017426 30.22521815899144 21.42032177213789 80-81 21.227321026683473 27.999701480565015 29.135395317407898 21.637582175343613 82-83 19.42763905042769 27.790133980041336 31.773591955613114 21.00863501391786 84-85 18.75302063576514 29.178326151496815 30.363583012147526 21.70507020059052 86-87 18.62922079242138 29.892612469760728 29.6323006240979 21.84586611371999 88-89 18.589478277003128 29.329766693647535 27.90812300829203 24.17263202105731 90-91 19.644215896378707 26.62117223752577 29.47993140160386 24.254680464491667 92-93 21.112061017857794 29.074355360790825 29.57019652916259 20.24338709218879 94-95 19.910558721159273 27.765785487139706 29.9887195753443 22.33493621635672 96-97 17.108779439776136 28.39260548364694 31.88344508839548 22.615169988181446 98-99 19.15584596897387 29.571384113487998 27.78746029336584 23.485309624172295 100-101 18.379233738727123 27.11070113698438 29.781264024465777 24.72880109982272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1923.0 1 1157.5 2 482.0 3 564.5 4 465.5 5 463.5 6 760.0 7 1406.5 8 2180.5 9 2350.5 10 1827.0 11 1413.0 12 1305.0 13 1281.5 14 1339.0 15 1344.0 16 1391.0 17 1477.0 18 1566.5 19 1740.5 20 2006.5 21 2333.0 22 2663.5 23 3075.5 24 3588.0 25 4754.0 26 7489.0 27 10267.5 28 10931.0 29 11955.5 30 15123.0 31 18323.0 32 22284.0 33 27492.0 34 33255.0 35 36764.5 36 39614.5 37 43001.5 38 41034.0 39 36842.5 40 34269.5 41 31867.0 42 29182.5 43 27947.5 44 27786.5 45 27432.0 46 29805.5 47 54857.5 48 105832.0 49 99656.0 50 47469.5 51 22211.0 52 11547.0 53 7422.5 54 5877.5 55 4653.0 56 3724.5 57 3151.5 58 2643.5 59 2201.5 60 1801.5 61 1410.0 62 1150.0 63 874.0 64 548.0 65 384.5 66 270.5 67 181.5 68 123.5 69 77.0 70 55.5 71 33.0 72 31.0 73 37.5 74 33.5 75 21.0 76 12.5 77 10.5 78 9.5 79 5.0 80 3.0 81 3.0 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33658512811557 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.615048147411087E-4 16-17 0.0014722426418328101 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.569028888446652E-4 26-27 0.0010661067406375522 28-29 0.0 30-31 0.0026906503454185837 32-33 0.0 34-35 0.0105087664434273 36-37 0.026703435503588214 38-39 0.021677503726296895 40-41 0.013656319677690548 42-43 0.010051863554582634 44-45 0.0027921843207173985 46-47 0.0014214756541834027 48-49 6.599708394422942E-4 50-51 1.5230096294822172E-4 52-53 5.076698764940724E-5 54-55 0.0 56-57 0.0 58-59 0.00461979587609606 60-61 0.009696494641036784 62-63 0.004061359011952579 64-65 0.0019291455306774754 66-67 0.004315193950199616 68-69 0.003452155160159693 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21347518306575747 78-79 2.538349382470362E-4 80-81 0.0031983202219126564 82-83 6.599708394422942E-4 84-85 0.0 86-87 0.10884442152032914 88-89 0.4010592024303173 90-91 0.38780901865382195 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012691746912351812 100-101 6.092038517928869E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 984892.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.364085081452455 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45305543801189 16.46071181501996 2 13.200256988565695 6.168238547557726 3 5.231194579528671 3.6666622570122223 4 2.7287288059201624 2.5501700794291535 5 1.6624380059365242 1.9420671506670553 6 1.0784222744127474 1.511780986386774 7 0.7685888087390095 1.2570162024021285 8 0.5881410012676406 1.0993101114806234 9 0.42477923367265463 0.8932120340725868 >10 3.0303635513310683 14.573212112789294 >50 0.460020395919764 7.551377654182069 >100 0.3297030032182582 14.485991125989411 >500 0.02389152197233755 3.6201022726171352 >1k 0.01346613056622662 6.844387481027065 >5k 0.005212695703055465 9.19330802820952 >10k+ 0.001737565234351822 8.18245214115727 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35345 3.588718356936598 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20187 2.049666359357168 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14358 1.4578248173403785 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10732 1.089662622906877 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9664 0.9812243372877432 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9467 0.9612221441538769 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9233 0.9374631939339542 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9188 0.9328941650455076 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8335 0.8462856841156188 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8284 0.8411074513753792 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7619 0.7735873578016677 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6440 0.6538788009243653 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6352 0.6449438110980696 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5752 0.584023425918781 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5189 0.5268597978255484 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5059 0.5136603810367025 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4972 0.5048269251857056 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4932 0.5007655661737531 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4521 0.45903510232594036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.35709499112593057 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3497 0.3550643116199543 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3224 0.32734553636337793 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3205 0.3254163908327004 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3194 0.32429951710441346 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2960 0.3005405668844909 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2876 0.2920117129593905 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2802 0.2844981987872782 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2481 0.25190579271635877 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1860 0.18885319405579495 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1735 0.17616144714344312 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1735 0.17616144714344312 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1644 0.166921855391251 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1599 0.16235282650280436 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1414 0.1435690410725237 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1411 0.14326443914662723 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1320 0.13402484739443513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1286 0.13057269223427542 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1283 0.130268090308379 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1218 0.12366838191395606 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1205 0.12234844023507145 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1144 0.11615486774184379 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1124 0.1141241882358675 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1089 0.11057049910040899 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1074 0.10904748947092675 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1069 0.1085398195944327 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1045 0.10610300418726114 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1002 0.10173704324941212 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 992 0.10072170349642398 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.0153397529881449E-4 7 0.0 0.0 0.0 0.0 1.0153397529881449E-4 8 0.0 0.0 0.0 0.0 1.0153397529881449E-4 9 0.0 0.0 0.0 0.0 1.0153397529881449E-4 10-11 0.0 0.0 0.0 5.0766987649407247E-5 1.0153397529881449E-4 12-13 0.0 0.0 0.0 1.0153397529881449E-4 1.0153397529881449E-4 14-15 0.0 0.0 0.0 2.0306795059762899E-4 1.0153397529881449E-4 16-17 0.0 0.0 0.0 6.599708394422943E-4 1.0153397529881449E-4 18-19 0.0 0.0 0.0 8.630387900399232E-4 1.0153397529881449E-4 20-21 0.0 0.0 0.0 0.0013199416788845883 1.0153397529881449E-4 22-23 0.0 0.0 0.0 0.0025383493824703623 1.0153397529881449E-4 24-25 0.0 0.0 0.0 0.004873630814343095 1.0153397529881449E-4 26-27 0.0 0.0 0.0 0.010457999455777894 1.0153397529881449E-4 28-29 0.0 0.0 0.0 0.02431738708406607 1.0153397529881449E-4 30-31 0.0 0.0 0.0 0.06122498710518513 1.0153397529881449E-4 32-33 0.0 0.0 0.0 0.1311818960860683 1.0153397529881449E-4 34-35 0.0 0.0 0.0 0.22880681333587843 1.0153397529881449E-4 36-37 0.0 0.0 0.0 0.35613041836059184 1.0153397529881449E-4 38-39 0.0 0.0 0.0 0.5315811276769432 1.0153397529881449E-4 40-41 0.0 0.0 0.0 0.7176421374120208 1.0153397529881449E-4 42-43 0.0 0.0 0.0 0.9116735642080553 1.0153397529881449E-4 44-45 0.0 0.0 0.0 1.1543397651722218 1.0153397529881449E-4 46-47 0.0 0.0 0.0 1.4437115947738433 1.0153397529881449E-4 48-49 0.0 0.0 0.0 1.7011509891439873 1.0153397529881449E-4 50-51 0.0 0.0 0.0 1.9671192374392317 1.0153397529881449E-4 52-53 0.0 0.0 0.0 2.2232386901304917 1.0153397529881449E-4 54-55 0.0 0.0 0.0 2.5138289274356986 1.0153397529881449E-4 56-57 0.0 0.0 0.0 2.9347887890245836 1.0153397529881449E-4 58-59 0.0 0.0 0.0 3.3114798373831853 1.0153397529881449E-4 60-61 0.0 0.0 0.0 3.7339627086015525 1.0153397529881449E-4 62-63 0.0 0.0 0.0 4.152333453820317 1.0153397529881449E-4 64-65 0.0 0.0 0.0 4.587406537975737 1.0153397529881449E-4 66-67 0.0 0.0 0.0 5.109545005949891 1.0153397529881449E-4 68-69 0.0 0.0 0.0 5.700878878090187 1.0153397529881449E-4 70-71 0.0 0.0 0.0 6.328561913387457 1.0153397529881449E-4 72-73 0.0 0.0 0.0 6.906747135726556 1.0153397529881449E-4 74-75 0.0 0.0 0.0 7.588293944919849 1.0153397529881449E-4 76-77 0.0 0.0 0.0 8.24425419233784 1.0153397529881449E-4 78-79 0.0 0.0 0.0 9.153135572225178 1.0153397529881449E-4 80-81 0.0 0.0 0.0 10.079836164777458 1.0153397529881449E-4 82-83 0.0 0.0 0.0 10.879010084354427 1.0153397529881449E-4 84-85 0.0 0.0 0.0 11.761847999577618 1.0153397529881449E-4 86-87 0.0 0.0 0.0 12.664992709860574 1.0153397529881449E-4 88-89 0.0 0.0 0.0 13.662360949220828 1.0153397529881449E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCGTGC 40 5.420985E-4 47.44088 8 CCCGCAC 25 0.0015715963 38.216576 90-91 GTAAGCG 320 0.0 37.06507 94-95 CGTCAGA 65 0.0058793416 29.194386 2 GTATGGT 150 3.2647222E-8 28.563137 1 GACCGTG 120 5.433012E-6 27.673847 6 TGACCGT 120 5.433012E-6 27.673847 5 CGCTTAC 35 0.008195114 27.27594 82-83 GGATATG 460 0.0 26.9073 1 ACCGTGC 125 7.461318E-6 26.56689 7 GGCATTC 380 0.0 26.217327 7 CATTCCG 390 0.0 25.545088 9 CATGGGT 520 0.0 24.632763 4 GATTGGC 450 0.0 24.24756 9 GAGTACT 5030 0.0 24.239178 12-13 CCGTGCA 140 1.7963761E-5 23.72044 8 CTTTAGG 455 0.0 22.938448 2 CATATAG 295 5.456968E-12 22.514315 3 GTACTTT 5390 0.0 22.40019 14-15 TACTCCC 170 3.1824402E-6 22.325119 5 >>END_MODULE