FastQCFastQC Report
Fri 27 May 2016
SRR939143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983858
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116150.585475371725194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87170.4393963680868288No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64150.3233598372464158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.2966946222965555No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34800.17541578076656697No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23870.12032111169246994No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.11356659599628602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22480.11331456182851797No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22260.11220561149033852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186900.022.7094361
GTACATG114200.022.6573221
TACATGG112150.022.343852
GAGTACT95450.022.2761712-13
AGTACTT98500.021.49024812-13
ACATGGG114200.021.1546423
TACCTGG14050.020.566252
GTACTTT103250.020.5258214-15
GTAAGGT7450.020.3467644
GGTATCA143550.019.4469851
CGTACAC1951.0580352E-519.4337673
AGAGTAC165350.019.37268810-11
TATCAAC218050.019.356342
ATCAACG219850.019.2194083
TCAACGC220450.019.145614
CAACGCA221150.019.063595
TAAGGTG10250.018.9479245
AACGCAG225100.018.7860366
CATGGGG46400.018.7845784
TACTTTT114000.018.52795614-15
Produced by FastQC (version 0.11.5)