FastQCFastQC Report
Fri 27 May 2016
SRR939143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983858
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110560.5572979517687254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90700.4571899803312535No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64980.32754360443136554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62720.3161516600482494No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34970.17627269693697836No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24220.12208535086684631No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23390.1179015836818966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23170.11679263334371714No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22060.1111974748192663No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA194150.022.686551
GAGTACT97700.022.19700812-13
GGTATCA150150.021.2160681
GTACCGT1805.21887E-621.0890716
AGTACTT101100.020.79339612-13
GTACTTT103000.020.75536514-15
GTACATG114100.020.4073831
TACATGG112150.020.3086912
TATCAAC226050.019.3747982
ACATGGG114400.019.3285773
AGAGTAC171450.019.27775410-11
ATCAACG225850.019.2448863
TCAACGC226500.019.2106084
CAACGCA227350.019.1387845
CATGGGG45750.018.9801654
ACCGTAT1751.0021402E-418.9801658
AACGCAG231750.018.795896
ACTTTTT118500.018.05964316-17
TACTTTT116050.017.91025514-15
GTAAGGT6650.017.83854
Produced by FastQC (version 0.11.5)