##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939143_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1983858 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49094037980541 34.0 31.0 34.0 31.0 34.0 2 32.6943173352125 34.0 31.0 34.0 31.0 34.0 3 32.77727387746502 34.0 31.0 34.0 31.0 34.0 4 36.24309602804233 37.0 35.0 37.0 35.0 37.0 5 36.20980785923186 37.0 37.0 37.0 35.0 37.0 6 36.25285630322332 37.0 37.0 37.0 35.0 37.0 7 36.2077885614797 37.0 37.0 37.0 35.0 37.0 8 36.19582248326241 37.0 37.0 37.0 35.0 37.0 9 37.92843943467728 39.0 38.0 39.0 35.0 39.0 10-11 37.87375961384333 39.0 38.0 39.0 35.0 39.0 12-13 37.808013728805186 39.0 38.0 39.0 35.0 39.0 14-15 39.14672194280034 40.0 38.5 41.0 36.0 41.0 16-17 39.098302146625414 40.0 38.0 41.0 36.0 41.0 18-19 39.14594063688026 40.5 38.5 41.0 36.0 41.0 20-21 39.21514216239267 41.0 39.0 41.0 36.0 41.0 22-23 39.173524516371636 41.0 39.0 41.0 35.5 41.0 24-25 39.11161383526442 41.0 39.0 41.0 35.0 41.0 26-27 38.96920394504042 40.0 38.5 41.0 35.0 41.0 28-29 38.81654911793082 40.0 38.0 41.0 35.0 41.0 30-31 38.59720932647397 40.0 38.0 41.0 34.5 41.0 32-33 38.41469046675719 40.0 38.0 41.0 34.0 41.0 34-35 38.335276516766825 40.0 38.0 41.0 34.0 41.0 36-37 38.2123418107546 40.0 38.0 41.0 34.0 41.0 38-39 38.06541874468838 40.0 38.0 41.0 33.5 41.0 40-41 37.88489649964866 40.0 37.5 41.0 33.0 41.0 42-43 37.69262895832262 40.0 37.0 41.0 33.0 41.0 44-45 37.43231975272424 40.0 37.0 41.0 33.0 41.0 46-47 37.08391729650005 40.0 37.0 41.0 31.5 41.0 48-49 36.7182016051552 40.0 36.0 41.0 31.0 41.0 50-51 36.4448755908941 40.0 36.0 41.0 30.0 41.0 52-53 36.20451438560623 39.0 35.0 41.0 30.0 41.0 54-55 35.9648135098379 39.0 35.0 41.0 29.0 41.0 56-57 35.68630768936083 39.0 35.0 40.5 28.5 41.0 58-59 35.38804062589158 38.5 35.0 40.0 28.0 41.0 60-61 35.022574700406985 38.0 35.0 40.0 27.5 41.0 62-63 34.63750933786592 37.5 34.0 40.0 26.5 41.0 64-65 34.24235126707657 37.0 34.0 40.0 26.0 41.0 66-67 33.881873349806284 36.5 34.0 39.0 26.0 41.0 68-69 33.41809166785123 36.0 33.0 39.0 25.0 40.0 70-71 32.98963484281637 35.0 33.0 38.5 24.0 40.0 72-73 32.53749033448966 35.0 32.5 37.5 22.5 40.0 74-75 32.06021751556815 35.0 32.0 37.0 21.5 39.0 76-77 30.108094430145705 33.5 29.5 35.0 18.0 37.5 78-79 31.059299103060802 34.5 31.0 36.0 18.5 38.0 80-81 31.089464316498457 35.0 31.0 36.0 18.5 37.5 82-83 30.799042068535147 35.0 31.0 35.0 17.5 37.0 84-85 30.43335284077792 34.0 31.0 35.0 12.5 36.5 86-87 29.907659973647306 34.0 30.5 35.0 6.0 36.0 88-89 29.369462431282884 34.0 30.0 35.0 2.0 36.0 90-91 28.925191974425587 34.0 29.0 35.0 2.0 35.0 92-93 28.661964717232784 34.0 29.0 35.0 2.0 35.0 94-95 28.34171019296744 34.0 29.0 35.0 2.0 35.0 96-97 27.799891675714694 34.0 29.0 35.0 2.0 35.0 98-99 27.067216000338732 33.5 27.0 35.0 2.0 35.0 100-101 23.520519613803003 29.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1842094211038372 1101 2 0.18637835083710996 1101 3 0.2554980118972878 1101 4 0.11541676332839756 1101 5 0.13833566842761513 1101 6 0.17344834226354067 1101 7 0.1875363005459505 1101 8 0.1726396910620167 1101 9 0.3264366644018679 1101 10-11 0.19912473097505767 1101 12-13 0.18290786396261183 1101 14-15 0.26595648702144103 1101 16-17 0.329484634372605 1101 18-19 0.2993906870919716 1101 20-21 0.3934111995788001 1101 22-23 0.3342471541127594 1101 24-25 0.2996728852114927 1101 26-27 0.34146008956198415 1101 28-29 0.24664012091252374 1101 30-31 0.2907161773150406 1101 32-33 0.18655081963412812 1101 34-35 0.2764453851081967 1101 36-37 0.3145332030968362 1101 38-39 0.33226699603288523 1101 40-41 0.3829617402151726 1101 42-43 0.28451454738804927 1101 44-45 0.36347670420502 1101 46-47 0.45370543097605065 1101 48-49 0.40659991784199434 1101 50-51 0.3369741048709969 1101 52-53 0.26956946658358305 1101 54-55 0.35181426522816395 1101 56-57 0.31776101194066797 1101 58-59 0.4761981577145846 1101 60-61 0.37651559172190474 1101 62-63 0.3054380377197248 1101 64-65 0.18455513641851695 1101 66-67 0.21802335940377304 1101 68-69 0.21661361952551772 1101 70-71 0.3542641208991668 1101 72-73 0.24367898588248948 1101 74-75 0.3830979174621376 1101 76-77 0.22689898924700458 1101 78-79 0.2856403049378038 1101 80-81 0.20357946299391116 1101 82-83 0.2901032462790134 1101 84-85 0.2907096046184705 1101 86-87 0.4923557360505164 1101 88-89 0.46112008612633204 1101 90-91 0.4812054197748914 1101 92-93 0.28220789530419665 1101 94-95 0.2337117932813193 1101 96-97 0.22695735906621906 1101 98-99 0.1242668955768238 1101 100-101 -0.07855707250008237 1102 1 0.1387593005516905 1102 2 0.16747847953219264 1102 3 0.21892338282934531 1102 4 0.12650554089650967 1102 5 0.10351023945420224 1102 6 0.12156101842335687 1102 7 0.12700185471237546 1102 8 0.19164548172862794 1102 9 0.14472250953429722 1102 10-11 0.18107282681432935 1102 12-13 0.2051924098991691 1102 14-15 0.27535713649692184 1102 16-17 0.29242770485857505 1102 18-19 0.1878513236612207 1102 20-21 0.2490968345868012 1102 22-23 0.18267884347147145 1102 24-25 0.09798282852932516 1102 26-27 0.10041609200609258 1102 28-29 0.02841332735486901 1102 30-31 0.17809940581065575 1102 32-33 0.25091305650410334 1102 34-35 0.2572387762298334 1102 36-37 0.38551953276442674 1102 38-39 0.3890310493280822 1102 40-41 0.39062436317041005 1102 42-43 0.3710393392534428 1102 44-45 0.4392036906216319 1102 46-47 0.40043442067676693 1102 48-49 0.5316853268070574 1102 50-51 0.4853316200639952 1102 52-53 0.5183310365901619 1102 54-55 0.44652836866668366 1102 56-57 0.5520018333851127 1102 58-59 0.44797967852070286 1102 60-61 0.3425892528215755 1102 62-63 0.39560174129734804 1102 64-65 0.38089856127516697 1102 66-67 0.46322821234728906 1102 68-69 0.35986190308083366 1102 70-71 0.25691228861666104 1102 72-73 0.31932331846193307 1102 74-75 0.2980913541352166 1102 76-77 0.3692160395703361 1102 78-79 0.46796181244583224 1102 80-81 0.2601974945101233 1102 82-83 0.25600348738331746 1102 84-85 0.4122032451710176 1102 86-87 0.4560654974074616 1102 88-89 0.49339304215816426 1102 90-91 0.6003149563133974 1102 92-93 0.5602515769387608 1102 94-95 0.5629661087299986 1102 96-97 0.6109517802383344 1102 98-99 0.5323487877741826 1102 100-101 -0.06480561687417463 1103 1 0.08987746955879317 1103 2 0.09645043442775147 1103 3 0.11739826356506455 1103 4 0.08517377021439643 1103 5 0.07956650434010726 1103 6 0.10809259365603907 1103 7 0.0827790612490702 1103 8 0.14732531762664536 1103 9 0.13374028174128938 1103 10-11 0.16502673568484738 1103 12-13 0.1783603075667486 1103 14-15 0.2518716335097082 1103 16-17 0.1947451850716817 1103 18-19 0.2026401533015445 1103 20-21 0.28777215720818106 1103 22-23 0.2567156413111391 1103 24-25 0.21423215873736012 1103 26-27 0.24665380855248742 1103 28-29 0.3558167887925592 1103 30-31 0.26722245633244057 1103 32-33 0.38113772892567255 1103 34-35 0.3324502180470361 1103 36-37 0.3905010736518264 1103 38-39 0.37272972910024293 1103 40-41 0.4501597222851288 1103 42-43 0.40153523316311635 1103 44-45 0.35529055939434073 1103 46-47 0.4773271288551939 1103 48-49 0.4848810311685625 1103 50-51 0.3836725424058969 1103 52-53 0.49344693715644183 1103 54-55 0.5837610715103807 1103 56-57 0.6013979583877713 1103 58-59 0.520813801441399 1103 60-61 0.640201724761134 1103 62-63 0.5582189696450754 1103 64-65 0.6784228095148208 1103 66-67 0.6014620623941482 1103 68-69 0.4971668962471867 1103 70-71 0.3181554697213258 1103 72-73 0.22113860222948034 1103 74-75 0.10612573010154591 1103 76-77 0.20194862556926196 1103 78-79 0.37319008463040504 1103 80-81 0.40688872218273886 1103 82-83 0.3940853802213198 1103 84-85 0.39409260143896674 1103 86-87 0.5151978010905474 1103 88-89 0.43710691174996086 1103 90-91 0.6299814294190362 1103 92-93 0.517068288809007 1103 94-95 0.255257186746892 1103 96-97 0.3778848293674244 1103 98-99 0.11515986040913617 1103 100-101 -0.1102588211353499 1104 1 0.08166258282739847 1104 2 0.14880685298223284 1104 3 0.1867039809329185 1104 4 0.09268876482065735 1104 5 0.12261979452349436 1104 6 0.1338151395028575 1104 7 0.16046881313688033 1104 8 0.1407742928154221 1104 9 0.1563128815255439 1104 10-11 0.14845284140221793 1104 12-13 0.21300583075985458 1104 14-15 0.17862934656257323 1104 16-17 0.2406049477469736 1104 18-19 0.2388291500652997 1104 20-21 0.2588422758705349 1104 22-23 0.1652925560900016 1104 24-25 0.16129019541480716 1104 26-27 0.16852608471214126 1104 28-29 0.2104601544883522 1104 30-31 0.23781231609512332 1104 32-33 0.22949803097529298 1104 34-35 0.2881572299132742 1104 36-37 0.31917248681256893 1104 38-39 0.3759651120560079 1104 40-41 0.44839445799170363 1104 42-43 0.31373372291500345 1104 44-45 0.36447502541375343 1104 46-47 0.37040932950244354 1104 48-49 0.30493798909736114 1104 50-51 0.26738559526457806 1104 52-53 0.2954007667141596 1104 54-55 0.3175064868286128 1104 56-57 0.37546397780524643 1104 58-59 0.41512059755789465 1104 60-61 0.47726321343427003 1104 62-63 0.3893482037335261 1104 64-65 0.30488149344146365 1104 66-67 0.3266624938936076 1104 68-69 0.2879435952763103 1104 70-71 0.28487348482381236 1104 72-73 0.24084906609140688 1104 74-75 0.29046057368299216 1104 76-77 0.34750633862212155 1104 78-79 0.47804963155597235 1104 80-81 0.34812324213958945 1104 82-83 0.6002635894985602 1104 84-85 0.3312878549449003 1104 86-87 0.31113566516821933 1104 88-89 0.4410102558707827 1104 90-91 0.5239883334104114 1104 92-93 0.6582432618402905 1104 94-95 0.7257529796271491 1104 96-97 0.845628950187276 1104 98-99 0.6810563005385859 1104 100-101 0.35333384337382157 1105 1 0.012662477730181365 1105 2 0.08211832609485725 1105 3 0.07354426819863846 1105 4 0.04885117505011749 1105 5 0.10865643841909645 1105 6 0.12011018241755522 1105 7 0.023517893536251222 1105 8 0.05484288626436751 1105 9 0.09647725356968095 1105 10-11 0.10721759880281923 1105 12-13 0.1260258342630891 1105 14-15 0.1719443229156994 1105 16-17 0.18386926724251396 1105 18-19 0.165152078774355 1105 20-21 0.17869794236204228 1105 22-23 0.13928211643331423 1105 24-25 0.1275509065726368 1105 26-27 0.20690492513953274 1105 28-29 0.2050362974205555 1105 30-31 0.2175626051124624 1105 32-33 0.2700375300119049 1105 34-35 0.4270647793965452 1105 36-37 0.2683802640064741 1105 38-39 0.27147984318230556 1105 40-41 0.3018554494087695 1105 42-43 0.42721489299732696 1105 44-45 0.4360483307211638 1105 46-47 0.2878375305884475 1105 48-49 0.22848719211014412 1105 50-51 0.23044672681125888 1105 52-53 0.131499838355424 1105 54-55 0.17281494715441426 1105 56-57 0.17262172872484172 1105 58-59 0.3364166564123394 1105 60-61 0.3615070389728956 1105 62-63 0.24933320986419716 1105 64-65 0.16296830374098903 1105 66-67 0.15685804477817555 1105 68-69 0.18624452363503963 1105 70-71 0.22403635047454173 1105 72-73 0.19927835318195974 1105 74-75 0.010381260318133911 1105 76-77 0.1664686787867744 1105 78-79 0.15507510011439152 1105 80-81 0.06054138892536898 1105 82-83 0.09877779513877982 1105 84-85 0.08978146153099331 1105 86-87 0.14371145777638006 1105 88-89 0.39151773528924494 1105 90-91 0.535278436756002 1105 92-93 0.46415561076304357 1105 94-95 0.1966229743722927 1105 96-97 0.22184026565408388 1105 98-99 0.12512496405233975 1105 100-101 -0.30904079316497857 1106 1 0.1413516765293963 1106 2 0.10856025800015345 1106 3 0.15179891180849125 1106 4 0.027532799638883887 1106 5 0.10896613348919004 1106 6 0.15487648102871532 1106 7 0.16682948902013806 1106 8 0.09390240341450351 1106 9 0.21417913856598858 1106 10-11 0.20902426997364643 1106 12-13 0.20949861109779278 1106 14-15 0.12405607467317736 1106 16-17 0.10938733381969001 1106 18-19 0.19135306199566315 1106 20-21 0.19066613659762055 1106 22-23 0.22834877728815428 1106 24-25 0.26632900749878274 1106 26-27 0.28146085173722923 1106 28-29 0.2858920029266514 1106 30-31 0.2557564021166243 1106 32-33 0.36056817946479924 1106 34-35 0.2356300409731844 1106 36-37 0.34420187749356757 1106 38-39 0.3129088908578552 1106 40-41 0.18169174421802126 1106 42-43 0.2446543578356355 1106 44-45 0.4331406680148788 1106 46-47 0.3944847007262027 1106 48-49 0.36298243354180926 1106 50-51 0.4038791948549587 1106 52-53 0.46999533916679326 1106 54-55 0.4039642441250777 1106 56-57 0.35550043453847024 1106 58-59 0.35837016233003993 1106 60-61 0.27312394052080435 1106 62-63 0.36808082022098176 1106 64-65 0.441470945566401 1106 66-67 0.44878681545019106 1106 68-69 0.2512308456603378 1106 70-71 0.23518254780383785 1106 72-73 0.24060206148310925 1106 74-75 0.1603440350245222 1106 76-77 0.17333941593907198 1106 78-79 0.43867062489954733 1106 80-81 0.4103548857647823 1106 82-83 0.4623522735743393 1106 84-85 0.5202689097324082 1106 86-87 0.5966955217990453 1106 88-89 0.748753420233303 1106 90-91 0.8076331874810698 1106 92-93 0.842406702700508 1106 94-95 0.7592018275534187 1106 96-97 0.7283103633977213 1106 98-99 0.7593952662323886 1106 100-101 0.35413998776705924 1107 1 0.013010037002551655 1107 2 -0.005976310629442594 1107 3 -0.03473770270591814 1107 4 0.059617753279582075 1107 5 0.1030545535187386 1107 6 0.09093312999844727 1107 7 0.10357023879879534 1107 8 0.10040579315487008 1107 9 0.21429984690232118 1107 10-11 0.10665786516324971 1107 12-13 0.07769449335319933 1107 14-15 0.12801794126725952 1107 16-17 0.08623427564921826 1107 18-19 0.10867449969191512 1107 20-21 0.22576827722830473 1107 22-23 0.2677472743520539 1107 24-25 0.10422149208827136 1107 26-27 0.17632011050983465 1107 28-29 0.03495058218692293 1107 30-31 0.07798665418065553 1107 32-33 0.16283475874175934 1107 34-35 0.16075424281212491 1107 36-37 0.10798674343170944 1107 38-39 0.0020406042488190224 1107 40-41 0.12968678657283306 1107 42-43 0.20537187634337783 1107 44-45 0.01694549384551891 1107 46-47 -0.037094268596675306 1107 48-49 0.03394871550604961 1107 50-51 0.021918521265938296 1107 52-53 0.09612799006784911 1107 54-55 -0.014277627224338119 1107 56-57 0.058267236461041705 1107 58-59 -0.011547834213992303 1107 60-61 -0.02044147088344772 1107 62-63 0.055282894840111396 1107 64-65 0.035252980203175355 1107 66-67 0.13160193720657531 1107 68-69 0.12716627008961012 1107 70-71 0.11415223567241384 1107 72-73 0.024777511441030242 1107 74-75 0.1634567719178861 1107 76-77 0.07156064955219676 1107 78-79 0.18210244893547056 1107 80-81 0.17899180968881012 1107 82-83 0.008774926905214642 1107 84-85 0.12161711495847882 1107 86-87 0.2476966970758916 1107 88-89 0.30059165437723223 1107 90-91 0.3997279125006976 1107 92-93 0.40040497263458974 1107 94-95 0.4114315946984348 1107 96-97 0.3740836684493267 1107 98-99 0.5900080995881467 1107 100-101 0.27001435932765716 1108 1 -0.03923766880823365 1108 2 -0.010042199190223755 1108 3 0.028992992843669185 1108 4 -0.025928111542370402 1108 5 -0.05719352538927325 1108 6 -0.08124381033777439 1108 7 -0.05567129758279776 1108 8 0.00634557280200454 1108 9 -0.005976398796555316 1108 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2316 98-99 -0.3789052547257228 2316 100-101 -0.0013681225501649408 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 12.0 5 42.0 6 66.0 7 202.0 8 505.0 9 956.0 10 1702.0 11 2756.0 12 4102.0 13 6013.0 14 7277.0 15 9811.0 16 13370.0 17 19540.0 18 19112.0 19 11897.0 20 10563.0 21 10361.0 22 11090.0 23 11799.0 24 13188.0 25 15451.0 26 18138.0 27 21937.0 28 26588.0 29 32600.0 30 39878.0 31 48860.0 32 61179.0 33 79428.0 34 111711.0 35 167410.0 36 267415.0 37 457851.0 38 432401.0 39 48640.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19369198716668 19.57197650428089 15.386630828596878 21.84770067995555 2 15.386232280737836 21.047121316142587 40.38474527914801 23.181901123971574 3 19.744759957617937 25.407262011696403 30.1007935043738 24.747184526311862 4 13.141565575761973 17.236163072155367 37.203317979411835 32.41895337267083 5 13.377469556792876 38.0236387886633 33.77459475426164 14.824296900282178 6 30.871564396242068 36.47791323774182 17.4571466304544 15.193375735561718 7 27.655608415521677 33.822985314473115 20.69356778559756 17.827838484407653 8 26.060433760884095 34.90239724819014 19.588700400935956 19.448468589989808 9 26.50960905468033 17.64229093009681 20.701582472132582 35.14651754309028 10-11 25.032814848643405 27.007048891604136 26.632551321717585 21.327584938034878 12-13 26.48969835542665 24.485825094336388 27.42373698117506 21.6007395690619 14-15 22.989127147350594 25.645540971046053 26.824517098153077 24.54081478345028 16-17 21.568402592774248 28.911076150519627 27.41507424308947 22.105447013616654 18-19 21.47208153811451 27.262920849909833 30.252651715155952 21.012345896819706 20-21 22.59413224131969 25.938146782683035 30.320012823498455 21.14770815249882 22-23 22.084241916508137 26.03762466870109 30.317870533072426 21.560262881718348 24-25 22.012735861182843 26.1982148824985 30.391784245458446 21.39726501086021 26-27 21.58602643772255 26.278938942410445 30.675858641255914 21.459175978611093 28-29 21.358937988505225 27.062042747011127 30.28445080242638 21.294568462057264 30-31 21.703829313610935 26.416598027424108 30.156865453306324 21.72270720565863 32-33 21.46464111846715 26.531258789691602 30.10104553854157 21.90305455329968 34-35 22.132662788651892 26.564947337271843 29.959907464027374 21.342482410048895 36-37 22.19039513211912 26.12255620218163 30.251284988650152 21.4357636770491 38-39 21.6962950769337 26.66040142107467 30.087598317761394 21.555705184230238 40-41 21.96312461480485 26.654231191448762 29.87460755782811 21.508036635918273 42-43 21.69070311657174 26.734501077304813 29.467613807649922 22.107181998473525 44-45 21.86879113461923 26.72833766801349 29.5209235376535 21.881947659713788 46-47 22.330080203323565 27.084663880477844 28.890990320253774 21.69426559594482 48-49 22.066805656089894 27.189814320077588 28.736785755331567 22.00659426850095 50-51 21.857696253002363 27.450872165556454 28.83078652421674 21.860645057224446 52-53 22.288092768759853 27.53584871897301 28.287414796677684 21.888643715589453 54-55 21.817337732841764 27.255201733188567 28.547431318168943 22.38002921580073 56-57 22.03174319936205 27.489064237460543 28.21678769347403 22.262404869703374 58-59 22.386214871650363 27.12272294898428 28.26693840639069 22.224123772974668 60-61 21.95475572989147 27.34122802131117 28.874509144611626 21.829507104185733 62-63 21.826733461842466 27.455367944767957 28.79465725969061 21.923241333698968 64-65 21.56475889949693 27.644418344406247 28.552010767105884 22.238811988990935 66-67 21.79510057299614 27.4722840149351 28.182165085596562 22.5504503264722 68-69 21.912694520043168 27.37677267739124 28.342649661532228 22.367883141033364 70-71 22.13936736887604 27.315142342632974 28.09418015154818 22.4513101369428 72-73 21.877044627186017 27.310447622763323 28.34847554613284 22.46403220391782 74-75 21.51182191467333 28.087973030327774 27.96747549471787 22.43272956028103 76-77 21.710315109892537 27.38178800963351 28.225162568718353 22.68273431175559 78-79 21.87660908753411 27.53225484090401 28.096082893384803 22.495053178177074 80-81 22.02919963383258 27.66572806019986 27.82358231104874 22.48148999491882 82-83 21.844865982540984 27.592665962983016 28.137869572537383 22.424598481938617 84-85 21.748487479569476 27.73545983015413 27.918058630723223 22.59799405955317 86-87 21.701416484629394 27.698402966690335 27.824529692890632 22.775650855789642 88-89 21.557258986113542 27.75707253773252 27.56819486183891 23.117473614315028 90-91 21.63205005296925 27.470407684855346 27.75616585106418 23.14137641111122 92-93 21.86527463155125 27.499044790504158 27.780667769568186 22.855012808376408 94-95 21.517316259530674 27.337742923132602 27.92825897821316 23.216681839123567 96-97 21.287360284859098 27.190983427241267 28.209554312859087 23.31210197504055 98-99 21.329556458300043 27.416654778869326 27.60749803284258 23.64629072998805 100-101 21.359607227379154 26.799044280173096 28.262414654370676 23.578933838077067 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1893.0 1 1272.0 2 911.0 3 1140.5 4 925.5 5 846.0 6 1408.5 7 2971.0 8 4662.5 9 4994.0 10 3971.0 11 3014.0 12 2833.0 13 2992.0 14 3208.0 15 3287.0 16 3372.5 17 3561.5 18 3797.0 19 4042.0 20 4361.0 21 4950.5 22 5882.0 23 7160.5 24 8563.5 25 10363.5 26 13116.5 27 16514.5 28 19176.5 29 21726.0 30 24547.0 31 28366.5 32 33553.0 33 36662.0 34 38885.5 35 41679.0 36 45678.5 37 50380.0 38 52633.0 39 53767.0 40 55701.5 41 58832.5 42 60667.5 43 62215.5 44 64750.5 45 67568.5 46 69060.0 47 74179.0 48 85528.5 49 84174.5 50 73998.0 51 69282.5 52 65753.5 53 64052.5 54 63347.5 55 59531.0 56 54285.0 57 48653.0 58 43667.0 59 38886.5 60 32219.5 61 27025.5 62 23256.0 63 17449.5 64 12143.0 65 9195.0 66 6846.5 67 5410.0 68 4140.5 69 2805.5 70 1923.0 71 1298.0 72 990.0 73 814.5 74 618.5 75 488.5 76 367.5 77 228.0 78 146.0 79 87.0 80 55.0 81 44.5 82 20.5 83 7.5 84 4.0 85 4.5 86 4.5 87 3.5 88 1.5 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34362338433496753 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.073230039650015E-4 16-17 0.0018650528414836142 18-19 2.5203416776805595E-5 20-21 0.0 22-23 0.0 24-25 5.544751690897231E-4 26-27 0.001310577672393891 28-29 0.0 30-31 0.002822782679002227 32-33 0.0 34-35 0.009930146210061407 36-37 0.028832708792665602 38-39 0.021649735011276008 40-41 0.014391150979555997 42-43 0.010282994044936683 44-45 0.0025959519280109766 46-47 0.00126017083884028 48-49 7.056956697505568E-4 50-51 1.512205006608336E-4 52-53 2.5203416776805595E-5 54-55 0.0 56-57 0.0 58-59 0.004889462854700286 60-61 0.01025779062815988 62-63 0.004133360351396118 64-65 0.0019154596750372256 66-67 0.0037805125165208397 68-69 0.003276444180984728 70-71 2.5203416776805595E-5 72-73 0.0 74-75 0.0 76-77 0.21727865603284108 78-79 4.284580852056952E-4 80-81 0.003326851014538339 82-83 6.048820026433344E-4 84-85 2.5203416776805595E-5 86-87 0.10691289396720935 88-89 0.40204490442360286 90-91 0.3887122969486727 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0015626118401619472 100-101 6.552888361969455E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1983858.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.55264859900828 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8265912695716 39.5360949952198 2 14.665629966504573 15.7076665615861 3 5.03780291285729 8.093630673099204 4 2.2528688061477413 4.8258836606118924 5 1.2003273975144126 3.2140355661425737 6 0.7070434996555218 2.2718431248759114 7 0.45643000875135226 1.711012510809241 8 0.3198122770730948 1.3701435593395295 9 0.2418629795791139 1.1657162839058464 >10 1.1773994130845522 11.379958073508696 >50 0.07314021799263455 2.672772666046233 >100 0.0350751960885777 3.386742427859979 >500 0.0035720327628137663 1.237960084900701 >1k 0.0020680189679448085 1.770556770743632 >5k 2.8200258653792847E-4 1.0994245386398533 >10k+ 9.400086217930948E-5 0.5565585027107243 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11056 0.5572979517687254 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9070 0.4571899803312535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6498 0.32754360443136554 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6272 0.3161516600482494 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3497 0.17627269693697836 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2422 0.12208535086684631 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2339 0.1179015836818966 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2317 0.11679263334371714 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2206 0.1111974748192663 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.008136671072224E-4 2 0.0 0.0 0.0 0.0 1.008136671072224E-4 3 0.0 0.0 0.0 0.0 1.008136671072224E-4 4 0.0 0.0 0.0 0.0 1.008136671072224E-4 5 0.0 0.0 0.0 0.0 1.008136671072224E-4 6 0.0 0.0 0.0 0.0 1.008136671072224E-4 7 0.0 0.0 0.0 0.0 1.008136671072224E-4 8 0.0 0.0 0.0 0.0 1.008136671072224E-4 9 0.0 0.0 0.0 0.0 1.008136671072224E-4 10-11 0.0 0.0 0.0 2.52034167768056E-5 1.26017083884028E-4 12-13 0.0 0.0 0.0 5.04068335536112E-5 4.5366150198250077E-4 14-15 0.0 0.0 0.0 5.04068335536112E-5 6.048820026433344E-4 16-17 0.0 0.0 0.0 1.008136671072224E-4 8.065093368577792E-4 18-19 0.0 0.0 0.0 1.764239174376392E-4 8.569161704113903E-4 20-21 0.0 0.0 0.0 2.52034167768056E-4 0.0011089503381794463 22-23 0.0 0.0 0.0 5.292717523129176E-4 0.0012097640052866688 24-25 0.0 0.0 0.0 9.325264207418072E-4 0.0012097640052866688 26-27 0.0 0.0 0.0 0.0016634255072691694 0.0013609845059475022 28-29 0.0 0.0 0.0 0.004259377435280146 0.0014113913395011135 30-31 0.0 0.0 0.0 0.011366740966339325 0.0014870015898315304 32-33 0.0 0.0 0.0 0.025052196276144766 0.0015626118401619472 34-35 0.0 0.0 0.0 0.045088912613705213 0.0016130186737155583 36-37 0.0 0.0 0.0 0.07704684508669471 0.0016130186737155583 38-39 0.0 0.0 0.0 0.1270252205551002 0.0017138323408227806 40-41 0.0 0.0 0.0 0.18464023130687782 0.0017138323408227806 42-43 0.0 0.0 0.0 0.24149913955535124 0.0017138323408227806 44-45 0.0 0.0 0.0 0.31262318169949666 0.0017642391743763917 46-47 0.0 0.0 0.0 0.3984912226580733 0.0018398494247068085 48-49 0.0 0.0 0.0 0.49270159456977264 0.0019910699253676426 50-51 0.0 0.0 0.0 0.5975730117780607 0.002066680175698059 52-53 0.0 0.0 0.0 0.7027468699876704 0.0021170870092516703 54-55 0.0 0.0 0.0 0.8175232299892432 0.0021674938428052812 56-57 0.0 0.0 0.0 0.9578810580192736 0.002192697259582087 58-59 0.0 0.0 0.0 1.1121511721101007 0.0022179006763588926 60-61 0.0 0.0 0.0 1.2833327788581643 0.002318714343466115 62-63 0.0 0.0 0.0 1.4606136124662148 0.002570748511234171 64-65 0.0 0.0 0.0 1.6484042708701931 0.002621155344787782 66-67 0.0 0.0 0.0 1.8697154735873234 0.00274717242867181 68-69 0.0 0.0 0.0 2.1237659146975236 0.002873189512555838 70-71 0.0 0.0 0.0 2.401583177828252 0.0029235963461094494 72-73 0.0 0.0 0.0 2.6876419582449955 0.003074816846770283 74-75 0.0 0.0 0.0 2.9898813322324482 0.003276444180984728 76-77 0.0 0.0 0.0 3.3098639116307718 0.003326851014538339 78-79 0.0 0.0 0.0 3.687159060779552 0.0034276646816455616 80-81 0.0 0.0 0.0 4.092808053802237 0.003503274931975978 82-83 0.0 0.0 0.0 4.517082371822983 0.0036796988494136175 84-85 0.0 0.0 0.0 4.952849447893952 0.0036796988494136175 86-87 0.0 0.0 0.0 5.404041015032326 0.0037805125165208397 88-89 0.0 0.0 0.0 5.901531258789692 0.003881326183628062 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19415 0.0 22.68655 1 GAGTACT 9770 0.0 22.197008 12-13 GGTATCA 15015 0.0 21.216068 1 GTACCGT 180 5.21887E-6 21.089071 6 AGTACTT 10110 0.0 20.793396 12-13 GTACTTT 10300 0.0 20.755365 14-15 GTACATG 11410 0.0 20.407383 1 TACATGG 11215 0.0 20.308691 2 TATCAAC 22605 0.0 19.374798 2 ACATGGG 11440 0.0 19.328577 3 AGAGTAC 17145 0.0 19.277754 10-11 ATCAACG 22585 0.0 19.244886 3 TCAACGC 22650 0.0 19.210608 4 CAACGCA 22735 0.0 19.138784 5 CATGGGG 4575 0.0 18.980165 4 ACCGTAT 175 1.0021402E-4 18.980165 8 AACGCAG 23175 0.0 18.79589 6 ACTTTTT 11850 0.0 18.059643 16-17 TACTTTT 11605 0.0 17.910255 14-15 GTAAGGT 665 0.0 17.8385 4 >>END_MODULE