FastQCFastQC Report
Fri 27 May 2016
SRR939142_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939142_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946821
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.5730881267461159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83990.4314212760186992No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61300.3148722969394721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56660.2910385700585724No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33390.17151037511923284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23300.11968229231141435No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22370.11490527377709608No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22190.1139806895446474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21940.11269654477735755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA180450.023.3395441
GTACATG108000.023.0813481
TACATGG106350.022.9379962
GAGTACT92600.022.90701912-13
AGTACTT95850.021.6116312-13
ACATGGG107250.021.553033
GTACTTT99900.020.99804514-15
GGTATCA137350.020.7764551
TAAGGTG8550.020.4979425
TATCAAC208500.019.9922832
ATCAACG209300.019.9158673
TCAACGC209750.019.8505574
CAACGCA210850.019.7689555
CATGGGG44700.019.7102094
AACGCAG213900.019.5006876
AGAGTAC159500.019.23481410-11
GTAAGGT7900.019.187074
GGACCGA3105.817128E-918.3275476
TACTTTT113200.018.30089214-15
ACTTTTT117550.018.0880716-17
Produced by FastQC (version 0.11.5)