FastQCFastQC Report
Fri 27 May 2016
SRR939142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939142_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946821
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108210.5558292210737402No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88130.4526867133650192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64350.3305388631004083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60130.3088624994285556No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34070.17500324888626123No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23250.11942546335795638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22910.11767902647444217No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21790.11192605791698364No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20850.10709767359197378No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA950.001233210224.977872
GTACATG107250.022.7096561
TACATGG105250.022.4551662
GAGTACT95600.022.43943212-13
GTAAGCG2950.021.71804894-95
ACATGGG105400.021.38763
GTACTTT101900.020.95895814-15
GGTATCA148150.020.8478031
GTATCAA187200.020.5727581
GTAAGGT7950.020.2964864
AGTACTT100150.019.76134112-13
TAAGGTG10250.018.9831835
CATGGGA61150.018.5485734
AGAGTAC162400.018.5428710-11
ACTTTTT116550.018.26269116-17
CATGGGG44900.017.75714
TATCAAC216100.017.7226142
ATCAACG217100.017.6191233
TCAACGC217600.017.5786364
GCGATAC1350.00945073717.577023
Produced by FastQC (version 0.11.5)