##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939142_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1946821 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49826409310358 34.0 31.0 34.0 31.0 34.0 2 32.70747593127463 34.0 31.0 34.0 31.0 34.0 3 32.805925146687855 34.0 31.0 34.0 31.0 34.0 4 36.26149759017393 37.0 35.0 37.0 35.0 37.0 5 36.229164365907295 37.0 37.0 37.0 35.0 37.0 6 36.27434263345218 37.0 37.0 37.0 35.0 37.0 7 36.22573210377328 37.0 37.0 37.0 35.0 37.0 8 36.20631583489186 37.0 37.0 37.0 35.0 37.0 9 37.94167003540644 39.0 38.0 39.0 35.0 39.0 10-11 37.86223643570724 39.0 38.0 39.0 35.0 39.0 12-13 37.779363639492274 39.0 38.0 39.0 35.0 39.0 14-15 39.165645942795976 40.5 38.5 41.0 36.0 41.0 16-17 39.264942693755614 41.0 39.0 41.0 36.0 41.0 18-19 39.31416216488316 41.0 39.0 41.0 36.0 41.0 20-21 39.28843406764156 41.0 39.0 41.0 36.0 41.0 22-23 39.21211349168722 41.0 39.0 41.0 36.0 41.0 24-25 39.13452109875536 41.0 39.0 41.0 35.0 41.0 26-27 38.981181372093275 40.0 38.5 41.0 35.0 41.0 28-29 38.828644235910744 40.0 38.0 41.0 35.0 41.0 30-31 38.60690813382432 40.0 38.0 41.0 34.5 41.0 32-33 38.46192330984718 40.0 38.0 41.0 34.0 41.0 34-35 38.33745423950121 40.0 38.0 41.0 34.0 41.0 36-37 38.16980323306559 40.0 38.0 41.0 34.0 41.0 38-39 38.02300365570332 40.0 38.0 41.0 33.5 41.0 40-41 37.82559439208843 40.0 37.0 41.0 33.0 41.0 42-43 37.60066898805796 40.0 37.0 41.0 33.0 41.0 44-45 37.30114427571924 40.0 37.0 41.0 32.5 41.0 46-47 36.93451067149985 40.0 36.5 41.0 31.0 41.0 48-49 36.53161667148649 40.0 36.0 41.0 30.5 41.0 50-51 36.24529604930294 39.0 35.5 41.0 30.0 41.0 52-53 35.96497418098531 39.0 35.0 41.0 29.5 41.0 54-55 35.713700437790635 39.0 35.0 40.0 29.0 41.0 56-57 35.46126942333168 38.5 35.0 40.0 28.0 41.0 58-59 35.118537348836895 38.0 35.0 40.0 27.5 41.0 60-61 34.72549736210982 38.0 34.0 40.0 26.5 41.0 62-63 34.333313899942524 37.0 34.0 40.0 26.0 41.0 64-65 33.881770845907255 36.5 33.5 39.5 25.5 41.0 66-67 33.44528284829473 36.0 33.0 39.0 24.5 40.5 68-69 32.94849269655505 35.5 33.0 39.0 22.5 40.0 70-71 32.432675114969484 35.0 32.0 38.0 21.5 40.0 72-73 31.861976781635292 35.0 31.5 37.0 19.5 39.5 74-75 31.322970627499906 35.0 31.0 37.0 18.0 39.0 76-77 29.356248725486317 32.5 28.5 35.0 12.5 37.0 78-79 30.327831115444102 34.0 30.0 35.5 12.0 37.5 80-81 30.336649851218986 34.0 30.5 35.0 9.5 37.0 82-83 29.94939365252378 34.0 30.0 35.0 6.5 37.0 84-85 29.47575483313566 34.0 29.5 35.0 2.0 36.0 86-87 28.784617846222122 34.0 29.0 35.0 2.0 36.0 88-89 28.09690515974504 33.5 27.5 35.0 2.0 35.0 90-91 27.533192317115954 33.0 26.5 35.0 2.0 35.0 92-93 27.07737023588712 33.0 26.0 35.0 2.0 35.0 94-95 26.540998376327355 32.5 25.0 34.0 2.0 35.0 96-97 25.738707102501976 32.0 23.5 34.0 2.0 35.0 98-99 24.54532414639045 32.0 10.5 34.0 2.0 35.0 100-101 18.723582959090745 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04837518235535754 1101 2 0.12773731880950123 1101 3 0.1341962636354168 1101 4 0.11744989673460537 1101 5 0.09244834928528434 1101 6 0.13310027691866821 1101 7 0.12976292893235097 1101 8 0.13164431136615917 1101 9 0.1758975744289657 1101 10-11 0.19299668797882674 1101 12-13 0.131224973272424 1101 14-15 0.2787354180514541 1101 16-17 0.24448978963898327 1101 18-19 0.27585418657235294 1101 20-21 0.25411906982969157 1101 22-23 0.22837695270960978 1101 24-25 0.2350287111041709 1101 26-27 0.2633576967476401 1101 28-29 0.18559931988522038 1101 30-31 0.24628051480664226 1101 32-33 0.2827240524328616 1101 34-35 0.21895949994301844 1101 36-37 0.28274457899885874 1101 38-39 0.2539174851224004 1101 40-41 0.4054731589447087 1101 42-43 0.4757287101369201 1101 44-45 0.5696486701129899 1101 46-47 0.5819648418752763 1101 48-49 0.5451803035944991 1101 50-51 0.5205650184502488 1101 52-53 0.5019970823599067 1101 54-55 0.3260212451019484 1101 56-57 0.5029445361987541 1101 58-59 0.42958522322029324 1101 60-61 0.4205789819593164 1101 62-63 0.279614647038386 1101 64-65 0.210923041397983 1101 66-67 0.19627638367459355 1101 68-69 0.2965869069373497 1101 70-71 0.325795856822495 1101 72-73 0.4254138923821422 1101 74-75 0.26828250575827894 1101 76-77 0.46521953998099974 1101 78-79 0.4314481299097288 1101 80-81 0.5600315112953744 1101 82-83 0.3579858429519298 1101 84-85 0.38643515848992394 1101 86-87 0.4651310897075298 1101 88-89 0.46800362138106166 1101 90-91 0.5540220268917011 1101 92-93 0.41034788341517725 1101 94-95 0.0065635905456815635 1101 96-97 -0.011855320811342551 1101 98-99 -0.22198501713538477 1101 100-101 -1.0370807324455633 1102 1 0.11504299011122754 1102 2 0.16620365541611903 1102 3 0.0765724656055724 1102 4 0.11335909192767701 1102 5 0.07258813051873858 1102 6 0.09396870724842898 1102 7 0.12521294543974903 1102 8 0.20158133969995617 1102 9 0.2691645007592598 1102 10-11 0.1926214451819206 1102 12-13 0.2594574513264831 1102 14-15 0.25853468290473813 1102 16-17 0.26723476969680604 1102 18-19 0.2464590154204913 1102 20-21 0.23321080451459864 1102 22-23 0.14582831021625964 1102 24-25 0.23010299107414056 1102 26-27 0.3170595492291639 1102 28-29 0.3010312457398783 1102 30-31 0.19697986534036005 1102 32-33 0.23137816514328335 1102 34-35 0.306091864864662 1102 36-37 0.4630668467293262 1102 38-39 0.40987793716488596 1102 40-41 0.32894123845536427 1102 42-43 0.4153983911443291 1102 44-45 0.4423857338644339 1102 46-47 0.32662972866222617 1102 48-49 0.46729536949404604 1102 50-51 0.5059170537968072 1102 52-53 0.47612804143771115 1102 54-55 0.37192561809592206 1102 56-57 0.3022385201679896 1102 58-59 0.26509869525960283 1102 60-61 0.15401439888862 1102 62-63 0.0885497376052129 1102 64-65 0.2678182429971727 1102 66-67 0.32648003847360485 1102 68-69 0.3266501090158016 1102 70-71 0.38644743631488154 1102 72-73 0.25904308600841475 1102 74-75 0.16827947748307537 1102 76-77 0.23535913359305027 1102 78-79 0.3292175089783562 1102 80-81 0.4362101110670018 1102 82-83 0.48270436970920727 1102 84-85 0.5066380990109778 1102 86-87 0.6727985179415192 1102 88-89 0.4859920698278053 1102 90-91 0.6365242577759602 1102 92-93 0.7732022368734874 1102 94-95 0.5532370466931269 1102 96-97 0.40552214019714583 1102 98-99 0.14119619254864446 1102 100-101 -0.18881262607542126 1103 1 0.04913831778434741 1103 2 0.10822005104966337 1103 3 0.06546721027800118 1103 4 0.1145388992247689 1103 5 0.15194884706490797 1103 6 0.13950586471661097 1103 7 0.09100609871193654 1103 8 0.01372437155569628 1103 9 0.09372340475830754 1103 10-11 0.08737462810847774 1103 12-13 0.15023686171912942 1103 14-15 0.05710252355793699 1103 16-17 0.16048868641747305 1103 18-19 0.20167267306897685 1103 20-21 0.23425680785828717 1103 22-23 0.17868939492031188 1103 24-25 0.18938561276166155 1103 26-27 0.1645085429730031 1103 28-29 0.14277079799938264 1103 30-31 0.1319582360652518 1103 32-33 0.14798441777523408 1103 34-35 0.28999189235042167 1103 36-37 0.3817101030100787 1103 38-39 0.5328715082710502 1103 40-41 0.5328480617320182 1103 42-43 0.634795740659797 1103 44-45 0.6764573897427297 1103 46-47 0.6455713882473191 1103 48-49 0.5260446035491526 1103 50-51 0.7323333583995364 1103 52-53 0.5488805132648054 1103 54-55 0.4875032351058266 1103 56-57 0.46121321940065485 1103 58-59 0.27360619962832544 1103 60-61 0.404747404998524 1103 62-63 0.29259919053774297 1103 64-65 0.36090828533532715 1103 66-67 0.3544444343290536 1103 68-69 0.4029382202902596 1103 70-71 0.30571668706316757 1103 72-73 0.4339467597701052 1103 74-75 0.38661900351763734 1103 76-77 0.4126251011800264 1103 78-79 0.4741270329199665 1103 80-81 0.5323724434732178 1103 82-83 0.5311694539694507 1103 84-85 0.28030075292492285 1103 86-87 0.07331630299279723 1103 88-89 0.43202400248013006 1103 90-91 0.532726431924722 1103 92-93 0.24778626260419045 1103 94-95 0.23494785868297186 1103 96-97 0.09357548022120454 1103 98-99 -0.1958786607555716 1103 100-101 -0.6594184664209877 1104 1 0.07624027388647647 1104 2 0.0672683251412991 1104 3 0.11622608684522362 1104 4 0.07596206827006569 1104 5 0.04629220753067642 1104 6 0.10251835160852352 1104 7 0.10145715014407841 1104 8 0.07545953126246019 1104 9 0.1634584336869196 1104 10-11 0.21330702791182432 1104 12-13 0.16570622209286512 1104 14-15 0.1333770656527662 1104 16-17 0.14298060965554527 1104 18-19 0.1928230485718032 1104 20-21 0.2336312220811294 1104 22-23 0.21648867103901637 1104 24-25 0.1885458474842352 1104 26-27 0.24805883080603763 1104 28-29 0.2815837864497368 1104 30-31 0.26591638826680963 1104 32-33 0.22801671271387391 1104 34-35 0.2721219052102768 1104 36-37 0.34884134494240016 1104 38-39 0.2812012108960573 1104 40-41 0.31668115732357904 1104 42-43 0.21461234246211802 1104 44-45 0.2759249228550331 1104 46-47 0.32814162701431826 1104 48-49 0.45933654398212553 1104 50-51 0.404897100640639 1104 52-53 0.1741069532170485 1104 54-55 0.3892149758357135 1104 56-57 0.550314464699305 1104 58-59 0.5875953147688051 1104 60-61 0.5308685552166068 1104 62-63 0.3662534772761106 1104 64-65 0.46268729205102943 1104 66-67 0.4866325405402918 1104 68-69 0.5075292600492318 1104 70-71 0.3830158093964471 1104 72-73 0.23767448207480513 1104 74-75 0.13768717647417006 1104 76-77 -0.0552099815129381 1104 78-79 0.3083206160718639 1104 80-81 0.4527418675407553 1104 82-83 0.40132430499133775 1104 84-85 0.49580589565474753 1104 86-87 0.4774730200251973 1104 88-89 0.8326315457156319 1104 90-91 0.9222540079516328 1104 92-93 0.7031271879322887 1104 94-95 0.7107336174263388 1104 96-97 0.3180425592426914 1104 98-99 0.25040406102843704 1104 100-101 -0.47949754482248963 1105 1 0.038556265373358656 1105 2 0.08014273596344168 1105 3 0.0027666797462799764 1105 4 0.01456766372560736 1105 5 8.931026459180202E-5 1105 6 0.0020866546518334417 1105 7 0.03725084143689372 1105 8 5.675010360874921E-4 1105 9 0.13969430700279162 1105 10-11 0.075989541377119 1105 12-13 0.05623836501396795 1105 14-15 0.016385247586569562 1105 16-17 0.14461089480138867 1105 18-19 0.09381096403193823 1105 20-21 0.1443782329720591 1105 22-23 0.16105927964338207 1105 24-25 0.15443948082686632 1105 26-27 0.24008559656376605 1105 28-29 0.15657456572330375 1105 30-31 0.1556377143878862 1105 32-33 0.16852671071340097 1105 34-35 0.1551727377470158 1105 36-37 0.3010808658713984 1105 38-39 0.31999489399267134 1105 40-41 0.1987854803947684 1105 42-43 0.15266211404613017 1105 44-45 0.1842510187402624 1105 46-47 0.10176937801615793 1105 48-49 0.0887557763380542 1105 50-51 0.24099022648663038 1105 52-53 0.2873427861590372 1105 54-55 0.3943561189750824 1105 56-57 0.20990163721931765 1105 58-59 0.23261024855109014 1105 60-61 -0.014371288399864568 1105 62-63 0.053709859066096044 1105 64-65 -0.018749374535694585 1105 66-67 0.14033605479819045 1105 68-69 0.11391640791993751 1105 70-71 0.08649424686049656 1105 72-73 -0.17886451104919132 1105 74-75 -0.13776255971439966 1105 76-77 -0.30678221023309504 1105 78-79 -0.45335145755795025 1105 80-81 -0.09587344496673822 1105 82-83 -0.039888593158114105 1105 84-85 -0.28863340373396795 1105 86-87 -0.3216414259616833 1105 88-89 -0.303485956240074 1105 90-91 -0.20733491612695332 1105 92-93 -0.011430692275069987 1105 94-95 -0.3736977286115106 1105 96-97 -0.4975395729171055 1105 98-99 -0.5084023777858597 1105 100-101 -1.2845575759255574 1106 1 0.07610663721447253 1106 2 0.0496320859170325 1106 3 -0.021209141769986672 1106 4 0.02039699276680551 1106 5 0.04962201231001018 1106 6 0.06433949486001467 1106 7 0.039883687989281214 1106 8 -0.004899679433151505 1106 9 0.0728498458493192 1106 10-11 0.05759559539688297 1106 12-13 0.10287534705045687 1106 14-15 0.06846371017336139 1106 16-17 0.043073745935707564 1106 18-19 -0.0390609945662348 1106 20-21 0.06088301462555279 1106 22-23 0.05740225460725412 1106 24-25 0.10857672720538147 1106 26-27 0.0931593068684009 1106 28-29 0.06884400073628427 1106 30-31 0.10365544292624662 1106 32-33 0.09426804323216942 1106 34-35 0.053138625877465984 1106 36-37 0.03987511378897324 1106 38-39 0.07667481692228506 1106 40-41 -0.07739774387300713 1106 42-43 -0.03826640199649489 1106 44-45 0.17408369023949177 1106 46-47 0.13698928120822984 1106 48-49 0.21498335132307744 1106 50-51 0.1920285032116098 1106 52-53 0.2959303773570383 1106 54-55 0.21740997425663267 1106 56-57 0.05621509399058766 1106 58-59 0.09600989257049264 1106 60-61 0.02921900496104257 1106 62-63 0.19650653893027936 1106 64-65 0.07397652040889824 1106 66-67 0.01859524508920174 1106 68-69 -0.1351698498194125 1106 70-71 -0.002287811952669472 1106 72-73 0.17211458243389544 1106 74-75 0.05325950403315005 1106 76-77 0.17078308224666472 1106 78-79 0.2132192627446905 1106 80-81 0.4282460023822061 1106 82-83 0.44498986825382403 1106 84-85 0.3314969756262087 1106 86-87 0.3557291345608924 1106 88-89 0.33506083817232124 1106 90-91 0.5177871217738144 1106 92-93 0.46356093689419 1106 94-95 0.34754978951060167 1106 96-97 -0.0862012092529767 1106 98-99 -0.09606494813020916 1106 100-101 -0.7366925783340044 1107 1 0.013682923907396116 1107 2 -0.012685077054769067 1107 3 0.031918194248490295 1107 4 0.02459234248620845 1107 5 -0.007505424122392412 1107 6 0.048349413524732654 1107 7 0.10777800790805969 1107 8 0.10950469861413126 1107 9 0.05669659188018272 1107 10-11 0.03711491949957235 1107 12-13 -0.029517070582230076 1107 14-15 0.008360813789380472 1107 16-17 0.048865816582484456 1107 18-19 0.05984965377303553 1107 20-21 0.03751627506581201 1107 22-23 -0.006789301697068595 1107 24-25 0.02909798706546951 1107 26-27 0.09170581525296484 1107 28-29 0.09454062236544303 1107 30-31 0.09751652583468484 1107 32-33 0.1454788678536616 1107 34-35 -0.03189258611865142 1107 36-37 0.20066961110568826 1107 38-39 0.26792960053026604 1107 40-41 0.21474082176980858 1107 42-43 0.19684123615633098 1107 44-45 0.11348960238846217 1107 46-47 0.28491224698939277 1107 48-49 0.18080454200912754 1107 50-51 0.12287761454834367 1107 52-53 0.08036231344915024 1107 54-55 0.10084753352874287 1107 56-57 0.09586450991623963 1107 58-59 0.11415929176048678 1107 60-61 0.029150109848963268 1107 62-63 0.05879748270963603 1107 64-65 0.2401911384454749 1107 66-67 0.16181465510158688 1107 68-69 -0.030928198121848993 1107 70-71 -0.05064646261261174 1107 72-73 -0.37067723159950816 1107 74-75 -0.23948419499283702 1107 76-77 -0.2963896520246081 1107 78-79 -0.34046313915647985 1107 80-81 -0.09787919147246171 1107 82-83 -0.36563838822566197 1107 84-85 -0.21116039531400332 1107 86-87 -0.11001576943223412 1107 88-89 -0.41692442887294234 1107 90-91 0.03868869938820296 1107 92-93 0.10910911326567785 1107 94-95 0.0012196396707082613 1107 96-97 -0.2922292526317918 1107 98-99 -0.022966118510328926 1107 100-101 -0.7893554999330412 1108 1 -0.04170160494943076 1108 2 -0.011345720199706477 1108 3 0.02891167170742648 1108 4 0.04536119618090595 1108 5 0.05515761872655389 1108 6 0.07657130012658797 1108 7 0.05085534156782501 1108 8 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-1.3916836214461341 2316 52-53 -1.2729029120101245 2316 54-55 -1.2878648294874608 2316 56-57 -1.1776906713392066 2316 58-59 -1.1197295805631384 2316 60-61 -1.0745576135247674 2316 62-63 -1.0035318935695159 2316 64-65 -1.0089599045044508 2316 66-67 -1.0166633897156387 2316 68-69 -0.9517965679113374 2316 70-71 -0.9046987122282886 2316 72-73 -0.5862299319521398 2316 74-75 -0.753477553993104 2316 76-77 -0.850924002977969 2316 78-79 -0.8700769267525494 2316 80-81 -0.6899466695179548 2316 82-83 -0.709245893528351 2316 84-85 -0.7766301336130539 2316 86-87 -0.8558929443417149 2316 88-89 -1.0625921641108853 2316 90-91 -1.3990114055109828 2316 92-93 -1.3936317395541913 2316 94-95 -1.0789902394161075 2316 96-97 -0.9249028502524936 2316 98-99 -0.5529734742324699 2316 100-101 0.37648431122599746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 17.0 5 46.0 6 102.0 7 218.0 8 518.0 9 965.0 10 1725.0 11 2766.0 12 4382.0 13 6021.0 14 7581.0 15 10296.0 16 13767.0 17 20085.0 18 20055.0 19 13326.0 20 11564.0 21 12344.0 22 13198.0 23 13872.0 24 15511.0 25 18531.0 26 21213.0 27 25240.0 28 30316.0 29 36589.0 30 44494.0 31 54822.0 32 69109.0 33 89717.0 34 127427.0 35 191779.0 36 303751.0 37 456181.0 38 294444.0 39 14841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.183869593428504 19.592293782023706 15.335377899090055 21.88845872545773 2 15.478875561749128 20.996486066258786 40.37027543877942 23.154362933212656 3 19.782352871681578 25.377371622763466 30.150589088570545 24.689686416984408 4 13.17414389920799 17.152218925109192 37.22735680373285 32.44628037194997 5 13.432565192177401 38.04140185461324 33.736999960448344 14.789032992761019 6 30.84469501818606 36.49066863363401 17.454301140166457 15.210335208013474 7 27.640753823797876 33.85390850006241 20.65074292911367 17.85459474702605 8 26.0585333731247 34.88245709287089 19.561274508544958 19.497735025459452 9 26.495758983491548 17.627968878494734 20.745666910311733 35.130605227701984 10-11 25.05625838225497 26.989974938630723 26.640815976404607 21.3129507027097 12-13 26.53993356348632 24.441409867676587 27.413229053929456 21.605427514907635 14-15 22.949961179855027 25.615568329233867 26.83043468545923 24.604035805451872 16-17 21.55815563783404 28.8832664877726 27.43155863848743 22.12701923590593 18-19 21.45732527917322 27.260763706968287 30.273805487923894 21.0081055259346 20-21 22.65187708577214 25.954543329869566 30.261282367510933 21.132297216847363 22-23 22.08839436188535 26.020317224850153 30.326645336165985 21.56464307709851 24-25 22.063929895932855 26.16451443893118 30.397340277991802 21.37421538714416 26-27 21.590382574516195 26.27013732574876 30.680855042075077 21.458625057659965 28-29 21.33126808557851 27.080154539157565 30.25933823893882 21.3292391363251 30-31 21.72993819154406 26.37399637665713 30.1484317253615 21.747633706437313 32-33 21.485442545935797 26.536344773439563 30.08366718965616 21.89454549096848 34-35 22.161037944269907 26.58513386436766 29.964704737055296 21.289123454307134 36-37 22.18504639428579 26.134475736478297 30.210298326599638 21.47017954263627 38-39 21.70299373694233 26.666944100007527 30.020573737286554 21.60948842576359 40-41 21.99519838942875 26.624357835314573 29.841031090261495 21.539412684995185 42-43 21.733837038346035 26.755715740233693 29.381802098177275 22.128645123243 44-45 21.864853244624484 26.732727432434583 29.491925744692544 21.91049357824839 46-47 22.3291116092148 27.083681676299555 28.860237225091584 21.726969489394065 48-49 22.10303543199397 27.171771522647276 28.734472578120286 21.99072046723847 50-51 21.885770847223608 27.449268185941484 28.82608744785048 21.83887351898443 52-53 22.270357675410324 27.52595128160216 28.240038503796704 21.963652539190814 54-55 21.776912207131524 27.30546362505849 28.52103506177507 22.396589106034913 56-57 22.042530371957763 27.471073989615373 28.192840582888866 22.293555055537993 58-59 22.35747895961124 27.123217868991105 28.29907568427333 22.220227487124326 60-61 21.949568246266445 27.316818965984417 28.87778958936844 21.8558231983807 62-63 21.80920996027059 27.327409236054446 28.861495139831145 22.00188566384382 64-65 21.592654699313247 27.635977261805913 28.53017706008701 22.24119097879383 66-67 21.730382448793097 27.48551649425835 28.183513986018475 22.600587070930075 68-69 21.913365578826465 27.36778868380445 28.329297509878092 22.389548227490998 70-71 22.089675516830276 27.36425420134075 28.10315185952058 22.44291842230839 72-73 21.9197347881495 27.31722639112687 28.261355306933716 22.501683513789917 74-75 21.534208846113742 28.05902545739952 27.91625424217224 22.490511454314497 76-77 21.73058686134539 27.33478096458308 28.147910313357237 22.786721860714295 78-79 21.863131153562612 27.534465087964 28.07082842075791 22.531575337715477 80-81 22.025016777416017 27.63395158293283 27.85551877465407 22.485512864997084 82-83 21.880285860898358 27.50558474559294 28.122719037857102 22.4914103556516 84-85 21.72174534792875 27.666333987562286 27.92950661617067 22.68241404833829 86-87 21.695756494945662 27.638936454897657 27.814673573765365 22.85063347639132 88-89 21.581427162470547 27.626693476170626 27.577689444428394 23.21418991693043 90-91 21.627444947180088 27.372214861585498 27.728164765008845 23.272175426225573 92-93 21.821266567393714 27.42134484885873 27.743048795960185 23.01433978778737 94-95 21.488981267409795 27.239638364287217 27.87066710293345 23.400713265369543 96-97 21.271889216288816 27.114723725171373 28.149436478607043 23.463950579932767 98-99 21.333555576330195 27.29376860086562 27.628211779426735 23.74446404337745 100-101 21.62087820363003 26.310871859935332 28.322644935445844 23.745605000988796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1710.0 1 1189.5 2 913.5 3 1087.5 4 840.5 5 778.5 6 1325.0 7 2825.0 8 4444.5 9 4781.5 10 3871.0 11 3003.5 12 2871.0 13 2923.0 14 2983.0 15 3140.5 16 3311.5 17 3451.0 18 3614.5 19 3876.5 20 4274.0 21 4858.0 22 5765.0 23 7010.5 24 8390.0 25 10170.0 26 13055.0 27 16430.5 28 18869.0 29 21269.0 30 24073.0 31 27738.0 32 32696.5 33 35779.5 34 37914.5 35 41150.5 36 45054.0 37 48961.5 38 51064.0 39 52546.5 40 54832.0 41 57604.5 42 59456.5 43 61106.5 44 63332.5 45 66091.5 46 68189.5 47 72916.5 48 83644.0 49 82951.5 50 72742.5 51 67877.5 52 64550.5 53 62956.5 54 62396.0 55 58745.0 56 53415.0 57 47685.5 58 42842.0 59 38039.5 60 31575.5 61 26638.0 62 22867.0 63 17283.0 64 12174.0 65 9268.5 66 6879.5 67 5375.5 68 4073.0 69 2796.5 70 1920.5 71 1322.0 72 1058.5 73 908.0 74 696.0 75 515.5 76 352.5 77 213.5 78 145.0 79 97.0 80 53.0 81 30.0 82 20.0 83 9.5 84 7.5 85 5.5 86 0.5 87 0.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4121077387186598 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011557302905608681 16-17 0.0023114605811217362 18-19 5.136579069159414E-5 20-21 0.0 22-23 0.0 24-25 5.136579069159414E-5 26-27 0.0032360448135704306 28-29 2.568289534579707E-5 30-31 0.0 32-33 2.568289534579707E-5 34-35 0.012456204242711579 36-37 0.026427699310825187 38-39 0.02093155970682461 40-41 0.009374256801215931 42-43 0.009194476533795352 44-45 0.004006531673944344 46-47 0.005367725127271588 48-49 0.003261727708916228 50-51 7.704868603739122E-4 52-53 0.0 54-55 0.0 56-57 2.568289534579707E-5 58-59 0.0015152908254020272 60-61 0.0026453382206170985 62-63 0.0013611934533272448 64-65 0.0026967040113086926 66-67 0.0019262171509347803 68-69 0.004571555371551878 70-71 1.0273158138318829E-4 72-73 0.0 74-75 0.0 76-77 0.09268956930298164 78-79 0.014690616137795926 80-81 3.852434301869561E-4 82-83 0.0 84-85 0.0 86-87 0.11069327894038539 88-89 0.4214563126245299 90-91 0.42148199551987575 92-93 0.0 94-95 0.0 96-97 2.568289534579707E-5 98-99 1.5409737207478243E-4 100-101 3.0819474414956486E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1946821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.85707585491619 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15082423233133 39.93546565385226 2 14.41721206121871 15.529377671949373 3 5.018422301228275 8.10832651647763 4 2.2563369275357443 4.860788362421645 5 1.18808239253026 3.199332176819627 6 0.6963704798142486 2.250268665268823 7 0.4589798487569443 1.7303518771266502 8 0.30463920165840275 1.3125581273678166 9 0.2255604116326184 1.0933221779247646 >10 1.1704971356694418 11.376882407911506 >50 0.07353879105510654 2.69797886452127 >100 0.03382164533979045 3.3709706714613876 >500 0.0032382570299045147 1.1535223204514413 >1k 0.0020953427840558606 1.7352078022159076 >5k 2.857285614621628E-4 1.090583335784333 >10k+ 9.524285382072093E-5 0.5550633684456172 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10821 0.5558292210737402 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8813 0.4526867133650192 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6435 0.3305388631004083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6013 0.3088624994285556 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3407 0.17500324888626123 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2325 0.11942546335795638 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2291 0.11767902647444217 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2179 0.11192605791698364 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2085 0.10709767359197378 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0546316276637657E-4 2 0.0 0.0 0.0 0.0 2.0546316276637657E-4 3 0.0 0.0 0.0 0.0 2.0546316276637657E-4 4 0.0 0.0 0.0 0.0 2.0546316276637657E-4 5 0.0 0.0 0.0 0.0 2.0546316276637657E-4 6 0.0 0.0 0.0 0.0 2.568289534579707E-4 7 0.0 0.0 0.0 0.0 2.568289534579707E-4 8 0.0 0.0 0.0 0.0 2.568289534579707E-4 9 0.0 0.0 0.0 0.0 2.568289534579707E-4 10-11 0.0 0.0 0.0 2.568289534579707E-5 2.568289534579707E-4 12-13 0.0 0.0 0.0 5.136579069159414E-5 7.448039650281151E-4 14-15 0.0 0.0 0.0 1.5409737207478243E-4 0.001027315813831883 16-17 0.0 0.0 0.0 1.7978026742057949E-4 0.0014382421393646359 18-19 0.0 0.0 0.0 2.568289534579707E-4 0.0014896079300562302 20-21 0.0 0.0 0.0 3.081947441495648E-4 0.0015409737207478244 22-23 0.0 0.0 0.0 5.907065929533326E-4 0.0015923395114394185 24-25 0.0 0.0 0.0 0.001078681604523477 0.0015923395114394185 26-27 0.0 0.0 0.0 0.001849168464897389 0.0017464368835142007 28-29 0.0 0.0 0.0 0.00446882379016869 0.001797802674205795 30-31 0.0 0.0 0.0 0.01158298580095448 0.001797802674205795 32-33 0.0 0.0 0.0 0.02516923743888113 0.001849168464897389 34-35 0.0 0.0 0.0 0.0447396036923785 0.001849168464897389 36-37 0.0 0.0 0.0 0.0780760018512231 0.001849168464897389 38-39 0.0 0.0 0.0 0.13039205967061174 0.0019005342555889833 40-41 0.0 0.0 0.0 0.18812720840796354 0.0019005342555889833 42-43 0.0 0.0 0.0 0.24688967295914727 0.0019262171509347803 44-45 0.0 0.0 0.0 0.3204198023341642 0.0020289487323179685 46-47 0.0 0.0 0.0 0.40653455042862185 0.00210599741835536 48-49 0.0 0.0 0.0 0.502331750068445 0.002157363209046954 50-51 0.0 0.0 0.0 0.6042928445912592 0.002157363209046954 52-53 0.0 0.0 0.0 0.7130085405900183 0.0023628263718133307 54-55 0.0 0.0 0.0 0.8317662486689839 0.0024141921625049248 56-57 0.0 0.0 0.0 0.9726882954313725 0.002491240848542316 58-59 0.0 0.0 0.0 1.137289971702586 0.002568289534579707 60-61 0.0 0.0 0.0 1.3168904588557448 0.002568289534579707 62-63 0.0 0.0 0.0 1.5013706961246052 0.0026196553252713015 64-65 0.0 0.0 0.0 1.6889842466256528 0.0026196553252713015 66-67 0.0 0.0 0.0 1.910293755820386 0.0026453382206170985 68-69 0.0 0.0 0.0 2.168612317208413 0.0026710211159628955 70-71 0.0 0.0 0.0 2.451663506814443 0.0027480698020002866 72-73 0.0 0.0 0.0 2.7375911806992015 0.0029535329647666633 74-75 0.0 0.0 0.0 3.0344854508966153 0.0031333132321872425 76-77 0.0 0.0 0.0 3.3524140123822375 0.003184679022878837 78-79 0.0 0.0 0.0 3.727769527861062 0.003184679022878837 80-81 0.0 0.0 0.0 4.133662005906039 0.003261727708916228 82-83 2.568289534579707E-5 0.0 0.0 4.555118318530569 0.003544239557719996 84-85 5.136579069159414E-5 0.0 0.0 4.988568543281586 0.003544239557719996 86-87 5.136579069159414E-5 0.0 0.0 5.439739965821203 0.0037497027204863725 88-89 5.136579069159414E-5 0.0 0.0 5.92941004848417 0.003929482987906952 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTATA 95 0.0012332102 24.97787 2 GTACATG 10725 0.0 22.709656 1 TACATGG 10525 0.0 22.455166 2 GAGTACT 9560 0.0 22.439432 12-13 GTAAGCG 295 0.0 21.718048 94-95 ACATGGG 10540 0.0 21.3876 3 GTACTTT 10190 0.0 20.958958 14-15 GGTATCA 14815 0.0 20.847803 1 GTATCAA 18720 0.0 20.572758 1 GTAAGGT 795 0.0 20.296486 4 AGTACTT 10015 0.0 19.761341 12-13 TAAGGTG 1025 0.0 18.983183 5 CATGGGA 6115 0.0 18.548573 4 AGAGTAC 16240 0.0 18.54287 10-11 ACTTTTT 11655 0.0 18.262691 16-17 CATGGGG 4490 0.0 17.7571 4 TATCAAC 21610 0.0 17.722614 2 ATCAACG 21710 0.0 17.619123 3 TCAACGC 21760 0.0 17.578636 4 GCGATAC 135 0.009450737 17.57702 3 >>END_MODULE