FastQCFastQC Report
Fri 27 May 2016
SRR939141_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939141_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856742
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.18934156462151638No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51650.18080036629139068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.14197991978274552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG73700.023.1416442
GTACATG76950.023.075131
ACATGGG75700.022.0295683
GTATCAA127350.021.6227151
GAGTACT69600.019.73311812-13
TAAGGTG16100.019.4212535
TCAACGC140250.019.1531124
ATCAACG140950.019.0916043
CAACGCA141300.019.0107865
TATCAAC143150.018.8643862
GGTATCA97850.018.825741
AACGCAG144200.018.6212836
CATGGGG46600.018.604774
AGAGTAC104950.018.29817610-11
GTGTAGC19000.017.741321
GTACTTT75800.017.71387914-15
AGTACTT75550.017.4581312-13
ACGCAGA157700.016.9376987
CGCAGAG158000.016.8755638
CGTACAC2858.8311936E-716.6231883
Produced by FastQC (version 0.11.5)