##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939141_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2856742 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795031892974585 33.0 31.0 34.0 30.0 34.0 2 31.90904183856995 34.0 31.0 34.0 30.0 34.0 3 31.97047615780494 34.0 31.0 34.0 30.0 34.0 4 35.41638936942853 37.0 35.0 37.0 33.0 37.0 5 35.45832420288566 37.0 35.0 37.0 33.0 37.0 6 35.529169242444716 37.0 35.0 37.0 33.0 37.0 7 35.4360453271594 37.0 35.0 37.0 33.0 37.0 8 35.41672646672328 37.0 35.0 37.0 33.0 37.0 9 37.063657831193716 39.0 37.0 39.0 33.0 39.0 10-11 36.95873113497824 39.0 37.0 39.0 33.0 39.0 12-13 36.85404824796919 39.0 37.0 39.0 33.0 39.0 14-15 38.117765797541395 40.0 38.0 41.0 33.0 41.0 16-17 38.04398104554069 40.0 38.0 41.0 33.0 41.0 18-19 37.951474616888746 40.0 38.0 41.0 33.0 41.0 20-21 37.83076018065334 40.0 38.0 41.0 32.0 41.0 22-23 37.742693774936626 40.0 38.0 41.0 32.0 41.0 24-25 37.780599368091345 40.0 38.0 41.0 32.0 41.0 26-27 37.691611808136685 40.0 38.0 41.0 32.0 41.0 28-29 37.563083925674775 40.0 38.0 41.0 32.0 41.0 30-31 37.39878277422322 40.0 37.5 41.0 31.5 41.0 32-33 37.19494917636945 40.0 37.0 41.0 31.0 41.0 34-35 36.87960183313719 40.0 37.0 41.0 30.0 41.0 36-37 36.618262517231166 40.0 36.0 41.0 30.0 41.0 38-39 36.367213945116504 39.5 36.0 41.0 30.0 41.0 40-41 36.21400392475064 39.0 36.0 41.0 29.5 41.0 42-43 36.02614884368277 39.0 35.0 41.0 28.5 41.0 44-45 35.78982158696866 39.0 35.0 41.0 27.5 41.0 46-47 35.46860462022822 39.0 35.0 41.0 26.0 41.0 48-49 35.472190348305865 39.0 35.0 41.0 26.0 41.0 50-51 34.4964879572604 38.0 34.0 40.0 25.0 40.5 52-53 34.67145983081426 38.5 34.5 39.5 25.0 40.5 54-55 35.18768478217494 39.0 35.0 40.5 26.0 41.0 56-57 35.063356088859265 39.0 35.0 41.0 25.0 41.0 58-59 34.8656227968784 39.0 35.0 41.0 25.0 41.0 60-61 34.565596928249036 38.0 34.0 40.0 24.0 41.0 62-63 34.19875263499469 38.0 34.0 40.0 23.0 41.0 64-65 33.830332070589506 37.0 34.0 40.0 22.0 41.0 66-67 33.43431555947299 37.0 33.5 39.5 21.5 41.0 68-69 33.027784798207186 36.0 33.0 39.0 20.0 41.0 70-71 32.61149431765277 35.5 32.5 39.0 18.5 40.5 72-73 32.1504570591254 35.0 32.0 38.5 17.5 40.0 74-75 31.693572608236934 35.0 31.5 37.0 13.5 39.5 76-77 31.248972955905714 35.0 31.0 37.0 9.5 39.0 78-79 30.812042704591455 35.0 31.0 36.0 7.0 39.0 80-81 30.37784073605527 35.0 31.0 36.0 4.0 37.5 82-83 29.93615069194208 34.0 30.0 35.5 2.0 37.0 84-85 29.555161439149913 34.0 30.0 35.0 2.0 37.0 86-87 29.171057974433815 34.0 29.5 35.0 2.0 36.0 88-89 28.750990989035763 34.0 29.0 35.0 2.0 36.0 90-91 28.359521615882706 34.0 29.0 35.0 2.0 35.5 92-93 27.934081201592583 34.0 28.0 35.0 2.0 35.0 94-95 27.456845420412485 34.0 27.0 35.0 2.0 35.0 96-97 26.87066105374584 34.0 25.5 35.0 2.0 35.0 98-99 26.13639296093242 33.0 24.5 35.0 2.0 35.0 100-101 23.606242355802518 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19836110515691274 1101 2 0.21957969143428002 1101 3 0.24762501566679163 1101 4 0.14558011516152192 1101 5 0.27385117089571054 1101 6 0.2949004057741149 1101 7 0.19328612846103255 1101 8 0.3526881447947332 1101 9 0.4494413037919003 1101 10-11 0.37955619122336515 1101 12-13 0.4598932401131819 1101 14-15 0.48336681757919564 1101 16-17 0.5204944717943576 1101 18-19 0.5797660770796185 1101 20-21 0.5894871593159934 1101 22-23 0.6022739562546988 1101 24-25 0.5780174168784811 1101 26-27 0.5732042066856309 1101 28-29 0.5014179354287478 1101 30-31 0.5056351266921553 1101 32-33 0.4868108909226976 1101 34-35 0.46286354596520596 1101 36-37 0.49992936896529017 1101 38-39 0.4675575282390909 1101 40-41 0.3159710087716263 1101 42-43 0.39279454198226205 1101 44-45 0.3122634239094282 1101 46-47 0.3653163226053806 1101 48-49 0.41854650073853605 1101 50-51 0.4282355384167147 1101 52-53 0.4425299205250042 1101 54-55 0.5260461245758279 1101 56-57 0.446841922411501 1101 58-59 0.5349450052850884 1101 60-61 0.44581426072956276 1101 62-63 0.4580292027952524 1101 64-65 0.4164852150970617 1101 66-67 0.2434461641471799 1101 68-69 0.303134747239973 1101 70-71 0.38276792546189853 1101 72-73 0.2825593541120952 1101 74-75 0.0707005422792939 1101 76-77 0.19337543946736702 1101 78-79 0.20925351856596563 1101 80-81 0.17680813176604104 1101 82-83 0.173503782766403 1101 84-85 0.05445242445034282 1101 86-87 -0.06638474297114882 1101 88-89 0.027584266586135442 1101 90-91 -0.1001750077655359 1101 92-93 0.08746884988401504 1101 94-95 -0.14841217408308083 1101 96-97 -0.2286506897273668 1101 98-99 -0.4383626148115063 1101 100-101 -0.36836418614844746 1102 1 0.19199148448392123 1102 2 0.22860682952931555 1102 3 0.2461930979918563 1102 4 0.3350162485886585 1102 5 0.2933710493203634 1102 6 0.3910915241921842 1102 7 0.30722544702238963 1102 8 0.38926536599993966 1102 9 0.45305591615387897 1102 10-11 0.37818727015277176 1102 12-13 0.3426000406371088 1102 14-15 0.49953313897912466 1102 16-17 0.5739379406821215 1102 18-19 0.5023275416842736 1102 20-21 0.5849993395232076 1102 22-23 0.5895240265388324 1102 24-25 0.5089334620002504 1102 26-27 0.546983692011473 1102 28-29 0.4209162103906223 1102 30-31 0.45742086179937047 1102 32-33 0.47203648678312504 1102 34-35 0.552856219573485 1102 36-37 0.4335413124736931 1102 38-39 0.5366809976969122 1102 40-41 0.3993564883944032 1102 42-43 0.4823185467654696 1102 44-45 0.3347695681026579 1102 46-47 0.3565037616330855 1102 48-49 0.416773364952185 1102 50-51 0.5186458681755965 1102 52-53 0.4621160344539632 1102 54-55 0.4665289085269464 1102 56-57 0.42006755584316835 1102 58-59 0.44091617648426507 1102 60-61 0.39834737200391146 1102 62-63 0.3541575189429764 1102 64-65 0.3666434029713983 1102 66-67 0.35910517664544983 1102 68-69 0.3777904197845885 1102 70-71 0.3613981493217082 1102 72-73 0.16359779726357004 1102 74-75 0.0869920471629051 1102 76-77 0.08740235731720247 1102 78-79 0.1910926825670174 1102 80-81 0.17136321703332058 1102 82-83 0.027036029902244962 1102 84-85 0.2158017890429491 1102 86-87 0.2245188388635455 1102 88-89 0.17643471621022755 1102 90-91 0.07798424765022816 1102 92-93 0.10160340933532197 1102 94-95 -0.039758254431117024 1102 96-97 -0.19677797072266756 1102 98-99 -0.22294483558270173 1102 100-101 -0.27686056570606254 1103 1 0.37740701694404777 1103 2 0.4538999650130684 1103 3 0.5054059947600607 1103 4 0.4460699037007032 1103 5 0.4505923265012868 1103 6 0.3022507350795607 1103 7 0.37176611613674737 1103 8 0.3990153036774515 1103 9 0.5501923468365106 1103 10-11 0.48313096641336273 1103 12-13 0.5219112656555254 1103 14-15 0.5394404119039109 1103 16-17 0.5908679944395914 1103 18-19 0.6530344702498283 1103 20-21 0.6347620431073935 1103 22-23 0.5901416000369792 1103 24-25 0.6780110828572177 1103 26-27 0.6765140033476342 1103 28-29 0.7075729808108946 1103 30-31 0.6323009106275492 1103 32-33 0.6655536290787083 1103 34-35 0.6581711820575435 1103 36-37 0.6230633790309739 1103 38-39 0.7158115345871181 1103 40-41 0.7164282041561236 1103 42-43 0.6858510860129385 1103 44-45 0.5451571103861994 1103 46-47 0.6500441061053479 1103 48-49 0.5894369622754283 1103 50-51 0.4898907104792869 1103 52-53 0.5482245239388774 1103 54-55 0.5475545828814177 1103 56-57 0.5831898466986232 1103 58-59 0.557075566332955 1103 60-61 0.701769991331858 1103 62-63 0.6610560692307885 1103 64-65 0.5896758175738839 1103 66-67 0.6181001801713037 1103 68-69 0.5773654023751078 1103 70-71 0.5945252375961019 1103 72-73 0.5022458105104732 1103 74-75 0.4412285322874574 1103 76-77 0.543991367334641 1103 78-79 0.4515575235771614 1103 80-81 0.31886940689682675 1103 82-83 0.2930909000673836 1103 84-85 0.4402859880152725 1103 86-87 0.3419672210847118 1103 88-89 0.24717969132204942 1103 90-91 0.2734889593561185 1103 92-93 0.12266181791089892 1103 94-95 0.10974900543262933 1103 96-97 0.18656986789567753 1103 98-99 -0.021987769342526775 1103 100-101 -0.2447506683921823 1104 1 0.33578502999073123 1104 2 0.3450528302950069 1104 3 0.38754959158013236 1104 4 0.30877920390302904 1104 5 0.349956046533201 1104 6 0.28483269350322615 1104 7 0.35602946153309034 1104 8 0.3976954394139085 1104 9 0.5502511708705384 1104 10-11 0.48821219872785093 1104 12-13 0.5666785001783694 1104 14-15 0.5868473749538339 1104 16-17 0.6585220674524734 1104 18-19 0.660819157128266 1104 20-21 0.5511401554196311 1104 22-23 0.6497591457757395 1104 24-25 0.6599159521391798 1104 26-27 0.7095724977219717 1104 28-29 0.669332609876335 1104 30-31 0.6232055507408845 1104 32-33 0.5730422656233927 1104 34-35 0.4400191881066178 1104 36-37 0.47721807153445894 1104 38-39 0.6460459238527108 1104 40-41 0.5070043794419377 1104 42-43 0.5544095479115114 1104 44-45 0.41013205502420647 1104 46-47 0.2991088039642804 1104 48-49 0.5078268871890046 1104 50-51 0.5288921173541041 1104 52-53 0.4724283774033111 1104 54-55 0.5371666261219872 1104 56-57 0.5689398776425136 1104 58-59 0.6102183322635426 1104 60-61 0.5578271204984162 1104 62-63 0.5304671732476223 1104 64-65 0.44174899712322 1104 66-67 0.3478025183041069 1104 68-69 0.35761872562665786 1104 70-71 0.37822711984264146 1104 72-73 0.3601768834486023 1104 74-75 0.4135941040509401 1104 76-77 0.392709125942865 1104 78-79 0.4072313161219512 1104 80-81 0.46532991574923344 1104 82-83 0.43737936210076 1104 84-85 0.5133152603252036 1104 86-87 0.43085891500855666 1104 88-89 0.3874618383502728 1104 90-91 0.2099496581611362 1104 92-93 0.15746527210719563 1104 94-95 0.1341977445762268 1104 96-97 0.056973474299162774 1104 98-99 -0.14727760026019254 1104 100-101 -0.2071221286865672 1105 1 0.31485695253405055 1105 2 0.269969948283741 1105 3 0.38033415512340696 1105 4 0.3392291027177876 1105 5 0.38158190595726893 1105 6 0.27949134262402 1105 7 0.3067739507516265 1105 8 0.32637314755331914 1105 9 0.3176061258723806 1105 10-11 0.4426247270106316 1105 12-13 0.43607294995181434 1105 14-15 0.5683708663711258 1105 16-17 0.3489924990066555 1105 18-19 0.5156053197650721 1105 20-21 0.47229890093548477 1105 22-23 0.4855182243572287 1105 24-25 0.4467663647455211 1105 26-27 0.4664875127607857 1105 28-29 0.6037391711274154 1105 30-31 0.5042475399564665 1105 32-33 0.5638284057877243 1105 34-35 0.49084244924024745 1105 36-37 0.5304393815847419 1105 38-39 0.6363348606565822 1105 40-41 0.5605321725627661 1105 42-43 0.4706737307002058 1105 44-45 0.4504559424300396 1105 46-47 0.5466108117019033 1105 48-49 0.6032766263309526 1105 50-51 0.6297265260147569 1105 52-53 0.5780577011357764 1105 54-55 0.5508526549884678 1105 56-57 0.5276048770097361 1105 58-59 0.45579388765877127 1105 60-61 0.4788655200328762 1105 62-63 0.4789030598870241 1105 64-65 0.5745515268134866 1105 66-67 0.4268784030984065 1105 68-69 0.36073234524346987 1105 70-71 0.25242831230276863 1105 72-73 0.4342996588220842 1105 74-75 0.5592550252565509 1105 76-77 0.4300231810524551 1105 78-79 0.47301695361265317 1105 80-81 0.43245032914802906 1105 82-83 0.48877611471082005 1105 84-85 0.4623298130225528 1105 86-87 0.3425249646679802 1105 88-89 0.3221707430066836 1105 90-91 0.35614503971407885 1105 92-93 0.2869848994427038 1105 94-95 0.1310649050852426 1105 96-97 0.03906262139802408 1105 98-99 0.03297037159954286 1105 100-101 0.02443728911091725 1106 1 0.20999512223915673 1106 2 0.24111778195036848 1106 3 0.2772641376481104 1106 4 0.25477552434734463 1106 5 0.2896147936963942 1106 6 0.2999110859180334 1106 7 0.2717362044053928 1106 8 0.24896237733727133 1106 9 0.32480687028890287 1106 10-11 0.3896928161780622 1106 12-13 0.4109444778415039 1106 14-15 0.4319066486741434 1106 16-17 0.41000830804812693 1106 18-19 0.47305151703265125 1106 20-21 0.4670759333313441 1106 22-23 0.6189492109753871 1106 24-25 0.5759788275562343 1106 26-27 0.576938212683288 1106 28-29 0.6083394326261384 1106 30-31 0.5797755473669994 1106 32-33 0.46628966095358493 1106 34-35 0.492083026667558 1106 36-37 0.48235416607251835 1106 38-39 0.5745861264589678 1106 40-41 0.558663423622562 1106 42-43 0.44618050710477775 1106 44-45 0.4371580454908113 1106 46-47 0.5143201333047003 1106 48-49 0.5159214771463567 1106 50-51 0.4651820957554591 1106 52-53 0.5771777004631105 1106 54-55 0.5457899359342306 1106 56-57 0.5478259036935356 1106 58-59 0.5799145526447091 1106 60-61 0.5322268857681394 1106 62-63 0.6306810690492881 1106 64-65 0.5511932861701965 1106 66-67 0.5389695660950764 1106 68-69 0.5215850328926237 1106 70-71 0.4887837824048944 1106 72-73 0.4187873609545818 1106 74-75 0.3031017472032467 1106 76-77 0.3016902984984533 1106 78-79 0.4245965264420093 1106 80-81 0.500928030986227 1106 82-83 0.5884481201930427 1106 84-85 0.5657384755532604 1106 86-87 0.43603192045003425 1106 88-89 0.6324983311223669 1106 90-91 0.68230086829087 1106 92-93 0.5671788233109787 1106 94-95 0.5027180787352528 1106 96-97 0.6328660405008861 1106 98-99 0.6219194578254594 1106 100-101 0.4410113474842703 1107 1 0.11204812995937274 1107 2 0.17535398214760178 1107 3 0.1868814645325898 1107 4 0.15980707876936862 1107 5 0.2464301713527277 1107 6 0.17930266076483292 1107 7 0.25320145016827666 1107 8 0.17601454064804045 1107 9 0.20218788968647772 1107 10-11 0.22565512174036684 1107 12-13 0.22254388205269748 1107 14-15 0.19091153035415687 1107 16-17 0.25188028762864434 1107 18-19 0.33186691985569183 1107 20-21 0.2295190515930301 1107 22-23 0.30487048817281703 1107 24-25 0.36257258581405694 1107 26-27 0.3120127293333823 1107 28-29 0.19222929249391285 1107 30-31 0.33965344907243633 1107 32-33 0.3985189482521392 1107 34-35 0.22765604291796393 1107 36-37 0.3757883031291058 1107 38-39 0.5843600167890557 1107 40-41 0.5278984416465633 1107 42-43 0.4388877235272517 1107 44-45 0.42618264330355515 1107 46-47 0.6672812951061289 1107 48-49 0.6257676886619734 1107 50-51 0.5912463331278914 1107 52-53 0.6429213006673606 1107 54-55 0.5847474114745026 1107 56-57 0.6245918618730784 1107 58-59 0.6871409093457714 1107 60-61 0.622009001639654 1107 62-63 0.640785901654219 1107 64-65 0.5122609111146446 1107 66-67 0.3929800473687237 1107 68-69 0.4833535507917901 1107 70-71 0.5330946151515548 1107 72-73 0.5602027633802535 1107 74-75 0.4689387291580438 1107 76-77 0.5470572059929033 1107 78-79 0.451043231622581 1107 80-81 0.4598894007441814 1107 82-83 0.4852587029282809 1107 84-85 0.4573951141678734 1107 86-87 0.585169265136642 1107 88-89 0.4875542906330992 1107 90-91 0.3811640613771772 1107 92-93 0.4644714810549466 1107 94-95 0.3200023784813979 1107 96-97 0.4198920039389513 1107 98-99 0.3438893359192221 1107 100-101 0.25648337898769924 1108 1 0.14238924797827934 1108 2 0.1662028983225916 1108 3 0.24208554921737147 1108 4 0.16709630390496955 1108 5 0.12003277500198095 1108 6 0.20312892801690197 1108 7 0.30164923303798474 1108 8 0.3819967226552947 1108 9 0.3178373414576825 1108 10-11 0.2788406018391072 1108 12-13 0.36424751839202685 1108 14-15 0.40426853361190496 1108 16-17 0.42219020920478556 1108 18-19 0.38503018951272594 1108 20-21 0.37043692196169786 1108 22-23 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6469.0 9 7893.0 10 9335.0 11 10614.0 12 13003.0 13 17367.0 14 15701.0 15 15973.0 16 17836.0 17 21296.0 18 20482.0 19 16703.0 20 16830.0 21 17742.0 22 19669.0 23 21563.0 24 24374.0 25 27193.0 26 30839.0 27 36247.0 28 42143.0 29 49291.0 30 59412.0 31 72220.0 32 89831.0 33 116242.0 34 158737.0 35 234392.0 36 373990.0 37 588272.0 38 565343.0 39 103860.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05253764115323 18.725523284992462 14.273276651369397 23.948662422484915 2 15.069521471489624 19.69265113801436 41.02515038891722 24.2126770015788 3 19.806384578049794 23.327346732805463 29.24714263937244 27.6191260497723 4 12.894185149178986 16.01002727255523 37.11895698422033 33.97683059404546 5 14.042415371155528 36.82697214931467 34.444332278977946 14.686280200551854 6 32.8948147483785 35.514750165276546 17.31362463813458 14.276810448210373 7 29.00040994089143 31.967646203491856 20.647181043211972 18.38476281240474 8 25.83844737703344 35.521036782570974 19.768871154130515 18.87164468626507 9 25.773626776341672 17.39808447119752 20.540029590950482 36.288259161510325 10-11 25.052517512260508 26.217099477767817 27.38735711838267 21.343025891588997 12-13 25.976094026239394 23.363751856048424 27.74970201619939 22.9104521015128 14-15 22.466429396797768 25.092795222648974 26.040928702016092 26.39984667853717 16-17 22.322821763412954 28.16459399069941 26.88395840588108 22.62862584000656 18-19 22.29551270910885 27.18376491232004 28.534493149302598 21.98622922926851 20-21 22.82435429448874 26.295204291632835 28.73330392162703 22.147137492251392 22-23 22.332333981072466 26.296076943699266 28.609259106750795 22.762329968477474 24-25 22.451323238580752 26.379602068954032 28.76108978464898 22.407984907816232 26-27 22.16333033256325 26.572341895721458 28.622422219040562 22.64190555267473 28-29 22.072638590427438 27.245525823606116 28.390537171031134 22.29129841493532 30-31 22.344782150078675 26.676969550422804 28.303440952911618 22.674807346586906 32-33 21.984644444837688 26.627263618063985 28.502406740871812 22.885685196226515 34-35 22.7024385567183 26.7884262277638 28.21268087421197 22.296454341305928 36-37 22.731439981695736 26.40119153579066 28.355551522333293 22.511816960180315 38-39 22.322172671898638 26.78739840171489 28.294537797691678 22.595891128694795 40-41 22.682857889169238 26.710753488444038 28.269641756633852 22.33674686575287 42-43 22.34007890573636 26.713356458431182 28.035186676229934 22.91137795960253 44-45 22.492826573568482 26.815346850576958 28.151942113873407 22.539884461981146 46-47 22.887190620776497 27.064525688137124 27.646444848347357 22.401838842739025 48-49 22.630319791667034 27.075828148519204 27.673118032951816 22.620734026861953 50-51 22.532700851030228 27.22749789093426 27.80303497945101 22.436766278584503 52-53 22.8816944088062 27.09958415369693 27.561385474310125 22.457335963186743 54-55 22.438081083324306 27.0459162213072 27.80485025652424 22.711152438844255 56-57 22.567371283165258 27.226918766985005 27.581658991587005 22.624050958262735 58-59 22.708103181738686 26.92737184675837 27.606080660059394 22.758444311443554 60-61 22.310351522520442 27.08753668922415 28.02284505441118 22.57926673384422 62-63 22.308314188869616 27.106468302710983 28.10397896564687 22.481238542772537 64-65 22.122984361022965 27.298297156392938 27.82768104915062 22.75103743343348 66-67 22.16032618255247 27.204669701468386 27.639978470262932 22.995025645716215 68-69 22.495862845795635 26.908786282890752 27.874224882807752 22.721125988505865 70-71 22.53377182705954 26.890634795659253 27.55949252372418 23.016100853557028 72-73 22.374208277708302 26.82197703235838 27.864614388810615 22.939200301122703 74-75 22.24109644269317 27.371331202463743 27.57282625884278 22.814746096000302 76-77 22.379645147732006 26.93643153468594 27.606092527491466 23.077830790090587 78-79 22.416388603763792 27.024795509921475 27.67132912133529 22.887486764979442 80-81 22.5844271364568 27.017562799575696 27.486914538914753 22.91109552505275 82-83 22.478650905329634 27.038095134259876 27.71779136357418 22.765462596836315 84-85 22.386279948032982 27.18264281961157 27.5829815759061 22.848095656449345 86-87 22.431989037844723 27.153210786369687 27.51770737326986 22.897092802515726 88-89 22.279814434690447 27.146621182283255 27.302677689055166 23.270886693971132 90-91 22.28537162544277 26.994171081408027 27.485346787850197 23.23511050529901 92-93 22.509109950333027 26.828502513602263 27.69231418682711 22.970073349237598 94-95 22.253082814898832 26.706911303021393 27.83585197985458 23.2041539022252 96-97 22.271366687346628 26.370589684915867 28.053194930636533 23.304848697100976 98-99 22.126017379839514 26.652423260969517 27.626596227062894 23.59496313212808 100-101 22.340215613513656 25.960939835739786 28.148326621058374 23.550517929688187 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2306.0 1 1279.5 2 258.5 3 273.5 4 262.0 5 259.0 6 399.5 7 743.5 8 1200.5 9 1499.0 10 1523.0 11 1556.0 12 1763.0 13 2031.5 14 2243.5 15 2439.5 16 2687.5 17 2959.5 18 3181.5 19 3457.5 20 3994.5 21 4661.5 22 5599.5 23 7002.5 24 8972.5 25 11613.0 26 14942.5 27 19351.5 28 23654.5 29 27742.5 30 32473.0 31 38658.5 32 47386.0 33 52718.5 34 54727.0 35 59025.5 36 64769.5 37 70380.0 38 75494.0 39 80098.5 40 84528.0 41 89243.0 42 92191.5 43 94693.0 44 98854.0 45 105152.5 46 108456.5 47 112281.0 48 120624.0 49 118685.5 50 109043.5 51 103909.5 52 101798.5 53 100456.0 54 97806.5 55 90510.0 56 81611.5 57 73274.0 58 65886.5 59 57858.5 60 47571.0 61 39511.5 62 33205.5 63 24870.5 64 17305.5 65 12898.0 66 9697.0 67 7513.0 68 5814.0 69 4479.0 70 3391.5 71 2287.5 72 1672.5 73 1366.0 74 1006.5 75 790.0 76 633.0 77 419.0 78 274.0 79 182.0 80 125.0 81 91.5 82 80.0 83 61.5 84 40.5 85 30.0 86 22.0 87 20.0 88 16.5 89 16.0 90 11.5 91 7.0 92 6.5 93 4.5 94 5.5 95 6.5 96 4.5 97 2.0 98 2.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32880113079865103 2 0.1157612413021547 3 0.11016045551190833 4 0.11635632479236836 5 0.13350873127499788 6 0.08523695874531197 7 0.0935331226971144 8 0.09927392813211694 9 0.0871972337718982 10-11 0.08597206188028181 12-13 0.08961257264394194 14-15 0.09129280838101586 16-17 0.08051129573479159 18-19 0.09542338790132256 20-21 0.10511974830068659 22-23 0.11296084840703151 24-25 0.12635022693683923 26-27 0.12551010906830226 28-29 0.12081945096897094 30-31 0.11103557829163432 32-33 0.10454216726606742 34-35 0.32265776888497455 36-37 0.4791647268111716 38-39 0.4531035704309315 40-41 0.42982530449022 42-43 0.462852438197079 44-45 0.47030848428034455 46-47 0.47779953527479907 48-49 0.4895436829787219 50-51 0.47811457947550046 52-53 0.48112500183775786 54-55 0.4671755447289255 56-57 0.4441773180777263 58-59 0.4252921684912393 60-61 0.38678676618329555 62-63 0.4555189093029752 64-65 0.48688330972835486 66-67 0.4957395522591819 68-69 0.5079387638085624 70-71 0.5000101514242448 72-73 0.4917139874724424 74-75 0.4984874377875216 76-77 0.47561172832548404 78-79 0.4536986539211451 80-81 0.4562015050711615 82-83 0.46952087377859114 84-85 0.4690658099331336 86-87 0.4727588280635773 88-89 0.4852205764468755 90-91 0.499747614590327 92-93 0.4938667895105683 94-95 0.4974197879962559 96-97 0.49761231500779557 98-99 0.49087386960390544 100-101 0.47928724400033323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2856742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.833608499774925 #Duplication Level Percentage of deduplicated Percentage of total 1 76.48092784269281 44.99648966396525 2 13.54971725443328 15.943575204599455 3 4.554197864449806 8.038196825626526 4 1.931606868453089 4.545736090961811 5 1.0236503682099252 3.0112522501956596 6 0.5914831539721725 2.087945298900653 7 0.40131363737312997 1.652751059878196 8 0.2706180352910297 1.2737148433032572 9 0.19555321150443364 1.035459097787298 >10 0.9246604403771121 9.594178347187714 >50 0.04775989699697734 1.8991714867185174 >100 0.024045661517542682 2.6016895153351474 >500 0.0022626541294233017 0.9381464397858983 >1k 0.0020840235398532727 2.0112696616809336 >5k 1.1908705942018704E-4 0.37042421407371484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.18934156462151638 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5165 0.18080036629139068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 0.14197991978274552 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 3.500491118903982E-5 6 0.0 0.0 0.0 0.0 7.000982237807964E-5 7 0.0 0.0 0.0 0.0 7.000982237807964E-5 8 0.0 0.0 0.0 0.0 7.000982237807964E-5 9 0.0 0.0 0.0 3.500491118903982E-5 7.000982237807964E-5 10-11 0.0 0.0 0.0 3.500491118903982E-5 7.000982237807964E-5 12-13 0.0 0.0 0.0 3.500491118903982E-5 1.750245559451991E-4 14-15 0.0 0.0 1.750245559451991E-5 3.500491118903982E-5 2.625368339177987E-4 16-17 0.0 0.0 3.500491118903982E-5 7.000982237807964E-5 3.325466562958783E-4 18-19 0.0 0.0 3.500491118903982E-5 1.0501473356711946E-4 3.500491118903982E-4 20-21 0.0 0.0 3.500491118903982E-5 1.0501473356711946E-4 5.600785790246371E-4 22-23 0.0 0.0 3.500491118903982E-5 2.450343783232787E-4 7.176006793753163E-4 24-25 0.0 0.0 3.500491118903982E-5 4.02556478673958E-4 7.701080461588761E-4 26-27 0.0 0.0 3.500491118903982E-5 5.250736678355974E-4 8.051129573479159E-4 28-29 0.0 0.0 3.500491118903982E-5 0.0018027529262355508 8.051129573479159E-4 30-31 0.0 0.0 3.500491118903982E-5 0.005985839813325809 8.051129573479159E-4 32-33 0.0 0.0 3.500491118903982E-5 0.014702062699396726 9.101276909150354E-4 34-35 0.0 0.0 3.500491118903982E-5 0.02879153945298525 9.801375132931151E-4 36-37 0.0 0.0 3.500491118903982E-5 0.051719756281806334 9.801375132931151E-4 38-39 0.0 0.0 3.500491118903982E-5 0.0924654729058487 9.801375132931151E-4 40-41 0.0 0.0 3.500491118903982E-5 0.1363266266257156 9.801375132931151E-4 42-43 0.0 0.0 3.500491118903982E-5 0.18165798661552215 0.0010151424244821548 44-45 0.0 0.0 3.500491118903982E-5 0.23626564807042427 0.0010676497912657146 46-47 0.0 0.0 3.500491118903982E-5 0.30466524453380806 0.0010851522468602345 48-49 0.0 0.0 3.500491118903982E-5 0.3789631685325451 0.001190166980427354 50-51 0.0 0.0 3.500491118903982E-5 0.4672980619180871 0.0012601768028054337 52-53 0.0 0.0 3.500491118903982E-5 0.5517999175284292 0.0013651915363725531 54-55 0.0 0.0 3.500491118903982E-5 0.6436002971216861 0.0014001964475615929 56-57 0.0 0.0 3.500491118903982E-5 0.7555460031042356 0.0014001964475615929 58-59 0.0 0.0 3.500491118903982E-5 0.8808460827054037 0.0014176989031561128 60-61 0.0 0.0 3.500491118903982E-5 1.022283426364719 0.0014352013587506328 62-63 0.0 0.0 3.500491118903982E-5 1.1675538077992342 0.0015402160923177523 64-65 0.0 0.0 3.500491118903982E-5 1.3179349062673493 0.001575221003506792 66-67 0.0 0.0 3.500491118903982E-5 1.4918918124212825 0.001592723459101312 68-69 0.0 0.0 3.500491118903982E-5 1.6999610045289355 0.0016277283702903517 70-71 0.0 0.0 3.500491118903982E-5 1.9283680500374203 0.0016627332814793915 72-73 0.0 0.0 3.500491118903982E-5 2.166768997690376 0.0017152406482629514 74-75 0.0 0.0 3.500491118903982E-5 2.4087404462846136 0.0017852504706410309 76-77 0.0 0.0 3.500491118903982E-5 2.6715748219475195 0.0018552602930191106 78-79 0.0 0.0 3.500491118903982E-5 2.9805106656463902 0.0019077676598026703 80-81 0.0 0.0 3.500491118903982E-5 3.3311723634825965 0.00196027502658623 82-83 0.0 0.0 3.500491118903982E-5 3.6869272758968084 0.0022053094049095086 84-85 0.0 0.0 3.500491118903982E-5 4.051345903830308 0.0022228118605040286 86-87 0.0 0.0 3.500491118903982E-5 4.432444371945384 0.0023103241384766285 88-89 0.0 0.0 3.500491118903982E-5 4.844259649628842 0.002380333960854708 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 7370 0.0 23.141644 2 GTACATG 7695 0.0 23.07513 1 ACATGGG 7570 0.0 22.029568 3 GTATCAA 12735 0.0 21.622715 1 GAGTACT 6960 0.0 19.733118 12-13 TAAGGTG 1610 0.0 19.421253 5 TCAACGC 14025 0.0 19.153112 4 ATCAACG 14095 0.0 19.091604 3 CAACGCA 14130 0.0 19.010786 5 TATCAAC 14315 0.0 18.864386 2 GGTATCA 9785 0.0 18.82574 1 AACGCAG 14420 0.0 18.621283 6 CATGGGG 4660 0.0 18.60477 4 AGAGTAC 10495 0.0 18.298176 10-11 GTGTAGC 1900 0.0 17.74132 1 GTACTTT 7580 0.0 17.713879 14-15 AGTACTT 7555 0.0 17.45813 12-13 ACGCAGA 15770 0.0 16.937698 7 CGCAGAG 15800 0.0 16.875563 8 CGTACAC 285 8.8311936E-7 16.623188 3 >>END_MODULE