FastQCFastQC Report
Fri 27 May 2016
SRR939141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939141_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856742
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57390.20089318531389955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54250.18990164320054104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43460.15213134402756706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.1000440361782758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1755.0240487E-927.1145785
GTATCAA136850.020.8767381
ATACCGA1856.6171124E-620.519146
GTACATG77950.020.1583041
TACATGG74850.019.6521822
GAGTACT79500.019.18977712-13
AGAGTAC111900.019.14625210-11
ACATGGG73100.018.564773
GTACTTT81400.018.50867814-15
TAAGGTG14950.018.4088945
GTAAGGT13700.018.3567664
GGTATCA109550.018.3424051
TCAACGC154050.018.1731934
CAACGCA154750.018.0909865
ATCAACG155150.018.0443443
TATCAAC156950.017.9583322
AGTACTT83500.017.81587612-13
GTCCGTA1600.00119996317.7939412
AACGCAG158700.017.6706076
AGGCGAC11000.017.2547325
Produced by FastQC (version 0.11.5)