##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939141_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2856742 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46836326136557 34.0 31.0 34.0 31.0 34.0 2 32.67795761745373 34.0 31.0 34.0 31.0 34.0 3 32.77901154531981 34.0 31.0 34.0 31.0 34.0 4 36.24470078151965 37.0 35.0 37.0 35.0 37.0 5 36.198003529895246 37.0 35.0 37.0 35.0 37.0 6 36.229899304872475 37.0 37.0 37.0 35.0 37.0 7 36.18140980179519 37.0 37.0 37.0 35.0 37.0 8 36.16076460527412 37.0 37.0 37.0 35.0 37.0 9 37.907710951846546 39.0 38.0 39.0 35.0 39.0 10-11 37.843953006606824 39.0 38.0 39.0 35.0 39.0 12-13 37.78065240053179 39.0 38.0 39.0 35.0 39.0 14-15 39.154695628796716 40.0 39.0 41.0 36.0 41.0 16-17 39.094576794124215 40.0 38.0 41.0 36.0 41.0 18-19 39.10808046368905 40.5 38.5 41.0 36.0 41.0 20-21 39.1701522223568 41.0 39.0 41.0 36.0 41.0 22-23 39.13590796788789 40.5 39.0 41.0 36.0 41.0 24-25 39.085002600864904 40.5 39.0 41.0 35.0 41.0 26-27 38.95521069105996 40.0 38.5 41.0 35.0 41.0 28-29 38.80966744634272 40.0 38.0 41.0 35.0 41.0 30-31 38.635169364261806 40.0 38.0 41.0 34.5 41.0 32-33 38.511646834050815 40.0 38.0 41.0 34.0 41.0 34-35 38.470456730079235 40.0 38.0 41.0 34.0 41.0 36-37 38.37024011968879 40.0 38.0 41.0 34.0 41.0 38-39 38.2406529886143 40.0 38.0 41.0 34.0 41.0 40-41 38.08058795649029 40.0 38.0 41.0 33.0 41.0 42-43 37.93879531298241 40.0 38.0 41.0 33.0 41.0 44-45 37.75235915598958 40.0 37.5 41.0 33.0 41.0 46-47 37.51947008165246 40.0 37.0 41.0 32.5 41.0 48-49 37.23836436752076 40.0 37.0 41.0 32.0 41.0 50-51 36.99930252714456 40.0 36.5 41.0 31.0 41.0 52-53 36.77161413246279 39.5 36.0 41.0 31.0 41.0 54-55 36.53302748375597 39.0 36.0 41.0 31.0 41.0 56-57 36.25311981270973 39.0 35.0 40.5 30.0 41.0 58-59 35.95212763350698 38.5 35.0 40.0 29.5 41.0 60-61 35.58954886370557 38.0 35.0 40.0 28.5 41.0 62-63 35.20051740759229 38.0 34.0 40.0 28.0 41.0 64-65 34.794908675687196 37.0 34.0 40.0 28.0 41.0 66-67 34.43578961628316 36.5 34.0 39.0 27.5 41.0 68-69 33.966185780865054 36.0 33.5 39.0 26.0 40.0 70-71 33.51968238643882 35.5 33.0 39.0 26.0 40.0 72-73 33.04216516577276 35.0 32.5 38.0 25.5 40.0 74-75 32.548551986843755 35.0 32.0 37.0 24.5 39.0 76-77 30.568324685953442 33.5 29.5 35.5 20.5 37.5 78-79 31.538647697271927 34.5 31.0 36.0 22.0 38.5 80-81 31.565773528025982 35.0 32.0 36.0 23.0 37.5 82-83 31.264185565234804 35.0 31.0 35.5 21.5 37.0 84-85 30.874405879144845 34.0 31.0 35.0 20.0 37.0 86-87 30.323849861135518 34.0 31.0 35.0 17.5 36.0 88-89 29.766521092909336 34.0 30.0 35.0 9.0 36.0 90-91 29.30567951183551 34.0 29.5 35.0 4.5 35.0 92-93 29.019022193813793 34.0 29.0 35.0 2.0 35.0 94-95 28.682339882285483 34.0 29.0 35.0 2.0 35.0 96-97 28.122681012146003 34.0 29.0 35.0 2.0 35.0 98-99 27.337201609385797 33.5 28.0 35.0 2.0 35.0 100-101 23.678070508292315 29.5 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1275543652449045 1101 2 0.1642346729428681 1101 3 0.20075395450756872 1101 4 0.1183637548444878 1101 5 0.12327660290713993 1101 6 0.18480200866726193 1101 7 0.14884670343612072 1101 8 0.1886449586936152 1101 9 0.2262038210284132 1101 10-11 0.2570822670995625 1101 12-13 0.21913877736028553 1101 14-15 0.2669378562683846 1101 16-17 0.2656875925354143 1101 18-19 0.28839944513301674 1101 20-21 0.352445830852254 1101 22-23 0.3936011318277792 1101 24-25 0.3079762794752483 1101 26-27 0.28763135101393544 1101 28-29 0.3453582635612946 1101 30-31 0.3624020597639799 1101 32-33 0.2749996748301129 1101 34-35 0.38925777538569406 1101 36-37 0.5033011026616592 1101 38-39 0.5306152076935007 1101 40-41 0.49844534353445624 1101 42-43 0.5456744342590127 1101 44-45 0.5124365959337283 1101 46-47 0.47309073398066914 1101 48-49 0.5145331403085933 1101 50-51 0.5306034526963046 1101 52-53 0.3919036781291112 1101 54-55 0.3629255894364505 1101 56-57 0.31028161395357756 1101 58-59 0.4121091977114375 1101 60-61 0.3555970495873595 1101 62-63 0.2328161683016461 1101 64-65 0.226866344022973 1101 66-67 0.2863753914448779 1101 68-69 0.16965669044448362 1101 70-71 0.22965493553592609 1101 72-73 0.18430250138346338 1101 74-75 0.23338030807587984 1101 76-77 0.17268055339875943 1101 78-79 0.30732974370175725 1101 80-81 0.25353136285113465 1101 82-83 0.28102086062229503 1101 84-85 0.14754659136129789 1101 86-87 0.2500098766886545 1101 88-89 0.5442338266627402 1101 90-91 0.4948174389889637 1101 92-93 0.4702769290775066 1101 94-95 0.302139109037185 1101 96-97 0.20824894262054627 1101 98-99 0.19412468951231077 1101 100-101 -0.2158721268949968 1102 1 0.07967176370863172 1102 2 0.06582555582813399 1102 3 0.09804714102804724 1102 4 0.09608433364886793 1102 5 0.03161476220142134 1102 6 0.10579098733845882 1102 7 0.0825937090731017 1102 8 0.06606781781312776 1102 9 0.1793362907414604 1102 10-11 0.18180666910145504 1102 12-13 0.10983772549514015 1102 14-15 0.1608344083945923 1102 16-17 0.1951519626924494 1102 18-19 0.1526338274812673 1102 20-21 0.17465516225261268 1102 22-23 0.21611929233989002 1102 24-25 0.2087880449737085 1102 26-27 0.22602990283698432 1102 28-29 0.04427805803365459 1102 30-31 0.16618707065315164 1102 32-33 0.13371529552642158 1102 34-35 0.14066734465087194 1102 36-37 0.16895885198893978 1102 38-39 0.2156708908617162 1102 40-41 0.17525899795937505 1102 42-43 0.11768619276144676 1102 44-45 0.1931425983927042 1102 46-47 0.21862591037763934 1102 48-49 0.21326605025291911 1102 50-51 0.38693751024766954 1102 52-53 0.31107531002942324 1102 54-55 0.18488908832939188 1102 56-57 0.10723335563964298 1102 58-59 0.09114247028301037 1102 60-61 0.16158094966340997 1102 62-63 0.14679195687661917 1102 64-65 0.006903902306980569 1102 66-67 0.05007075606080491 1102 68-69 0.044468834246458755 1102 70-71 0.08180012376136148 1102 72-73 0.017272430140621964 1102 74-75 0.056359202108140494 1102 76-77 0.08909717698805864 1102 78-79 0.13900346469254998 1102 80-81 0.17553784194662825 1102 82-83 0.18670490333949985 1102 84-85 0.09082373625802376 1102 86-87 0.09177471441328677 1102 88-89 0.08111534251974817 1102 90-91 0.24588190346276662 1102 92-93 0.31829754014133016 1102 94-95 0.2442816672758461 1102 96-97 0.23973412724432208 1102 98-99 -0.040036441874310214 1102 100-101 -0.29075225520725567 1103 1 0.16104892435246398 1103 2 0.15322397958716039 1103 3 0.14421947260504453 1103 4 0.07428119485589235 1103 5 0.11541700555991241 1103 6 0.09065808187963142 1103 7 0.14178060935648773 1103 8 0.17367725265331302 1103 9 0.1554760388016092 1103 10-11 0.1759921533927411 1103 12-13 0.20765876167483555 1103 14-15 0.24435659300354473 1103 16-17 0.18716243915024933 1103 18-19 0.1744204829958207 1103 20-21 0.20311220808491015 1103 22-23 0.2055709360524105 1103 24-25 0.1756097172509712 1103 26-27 0.17512624241889085 1103 28-29 0.15684330826865533 1103 30-31 0.24432142098906695 1103 32-33 0.2802155344554649 1103 34-35 0.30622193946676646 1103 36-37 0.44097847117183875 1103 38-39 0.3628748872875178 1103 40-41 0.45723012176731714 1103 42-43 0.3375454977761905 1103 44-45 0.41353706051349803 1103 46-47 0.3707001661340712 1103 48-49 0.41219174865727126 1103 50-51 0.31590368530448387 1103 52-53 0.3879396630089431 1103 54-55 0.32805091393567665 1103 56-57 0.40647534460934764 1103 58-59 0.38274846278672214 1103 60-61 0.3871412712411839 1103 62-63 0.34923707069915366 1103 64-65 0.2852972127960598 1103 66-67 0.40988785132410044 1103 68-69 0.44959799093681596 1103 70-71 0.2118674760068231 1103 72-73 0.1465625565626567 1103 74-75 0.045765006608299075 1103 76-77 0.09226840277255377 1103 78-79 0.21568791446187774 1103 80-81 0.21602265755054617 1103 82-83 0.2615863964168348 1103 84-85 0.27040591497694777 1103 86-87 0.5329041360919682 1103 88-89 0.33991851208150337 1103 90-91 0.39945142804734246 1103 92-93 0.4572496934226322 1103 94-95 0.2682886988854385 1103 96-97 0.21114301741419794 1103 98-99 0.18219190270846752 1103 100-101 -0.09041935577660709 1104 1 0.045364108428799454 1104 2 0.06887491041622695 1104 3 0.10874061433052873 1104 4 0.07585963976902121 1104 5 0.05501881586956614 1104 6 0.129628391673954 1104 7 0.1253604317324175 1104 8 0.09315940507374165 1104 9 0.18530717705892386 1104 10-11 0.08050159771395471 1104 12-13 0.10457469722820179 1104 14-15 0.13894162620848505 1104 16-17 0.10068697306169128 1104 18-19 0.13162676417715602 1104 20-21 0.11446867805462801 1104 22-23 0.14874339586517493 1104 24-25 0.10204551388157057 1104 26-27 0.1330799258450952 1104 28-29 0.12819922063555822 1104 30-31 0.12639363239284762 1104 32-33 0.23071710125812217 1104 34-35 0.17141762741997724 1104 36-37 0.1875452309357044 1104 38-39 0.2363013443772246 1104 40-41 0.25015711378807737 1104 42-43 0.10131644924268812 1104 44-45 0.12191783093678055 1104 46-47 0.16817407216022673 1104 48-49 0.16878494247144005 1104 50-51 0.14471272533301516 1104 52-53 0.09298737787904088 1104 54-55 0.21366497557590947 1104 56-57 0.3584649756478484 1104 58-59 0.3472904380242525 1104 60-61 0.28290370425224864 1104 62-63 0.3188452658674876 1104 64-65 0.21215795179749364 1104 66-67 0.3356494693772021 1104 68-69 0.09474935865688394 1104 70-71 -0.017437646708692967 1104 72-73 -0.04446788119845024 1104 74-75 0.0022151538522123815 1104 76-77 0.0336049093357218 1104 78-79 0.2049320011145852 1104 80-81 0.1733095154034956 1104 82-83 0.2617846211565116 1104 84-85 0.05755191377840774 1104 86-87 0.21360283693866577 1104 88-89 0.2759334350036369 1104 90-91 0.2871992396094498 1104 92-93 0.3008698286443803 1104 94-95 0.06505671650323208 1104 96-97 0.06075983398002549 1104 98-99 0.12804422619653621 1104 100-101 -0.2592855409031962 1105 1 0.10139388563201379 1105 2 0.05161221081043976 1105 3 0.10073036215394637 1105 4 0.09307355046343702 1105 5 0.11543368128904774 1105 6 0.11451092571679311 1105 7 0.14436415624834353 1105 8 0.11493759234982548 1105 9 0.10402307656677579 1105 10-11 0.10007023868552523 1105 12-13 0.15816221980416145 1105 14-15 0.20122362848174902 1105 16-17 0.18782529938322057 1105 18-19 0.2918384607420563 1105 20-21 0.2094734031867702 1105 22-23 0.2121008240212916 1105 24-25 0.10560902639932834 1105 26-27 0.22237960094051346 1105 28-29 0.13686191361787792 1105 30-31 0.16295190291168637 1105 32-33 0.12660407775310034 1105 34-35 0.32183310196162296 1105 36-37 0.15450597153364498 1105 38-39 0.25239177950334124 1105 40-41 0.29921820814998057 1105 42-43 0.21146939213068094 1105 44-45 0.2094161602861746 1105 46-47 0.1918720291763094 1105 48-49 0.3305485699536064 1105 50-51 0.30192227755133416 1105 52-53 0.16212244805819864 1105 54-55 0.18334384991814545 1105 56-57 0.20977603718811366 1105 58-59 0.13565212412172656 1105 60-61 0.13416361135816146 1105 62-63 0.14897883512231402 1105 64-65 0.10604369585499995 1105 66-67 0.2017768362307777 1105 68-69 0.27575976814074465 1105 70-71 0.25480137401210357 1105 72-73 0.1503577738444264 1105 74-75 0.04534617780431205 1105 76-77 0.16618774107962153 1105 78-79 0.22572938342685944 1105 80-81 0.13442637933506774 1105 82-83 0.1517969691864458 1105 84-85 0.3165750533241578 1105 86-87 0.35633213595141555 1105 88-89 0.4166325239224662 1105 90-91 0.5110480874223455 1105 92-93 0.5363261498540162 1105 94-95 0.32780378059353055 1105 96-97 0.3278015050327667 1105 98-99 0.3742400217160018 1105 100-101 -0.135034524615385 1106 1 0.02108336640429087 1106 2 0.02498222310060072 1106 3 -0.005090016528718877 1106 4 0.026745645634399295 1106 5 0.01679526844337431 1106 6 0.08559051185932276 1106 7 0.06825191079268933 1106 8 0.027667927743664222 1106 9 0.08362225199094553 1106 10-11 0.0817590916366342 1106 12-13 0.16340123328409817 1106 14-15 0.061636280759920226 1106 16-17 8.208471782111815E-4 1106 18-19 -0.009333308606535695 1106 20-21 0.06546916539389969 1106 22-23 0.11712927917481153 1106 24-25 0.1454010466241371 1106 26-27 0.1713210636236937 1106 28-29 0.15758253460027305 1106 30-31 0.12266750552890926 1106 32-33 0.10082914603566451 1106 34-35 0.032911552981062187 1106 36-37 0.17603393129703448 1106 38-39 0.08879405591562772 1106 40-41 0.10947502624580352 1106 42-43 0.2152634359576453 1106 44-45 0.21712792030153594 1106 46-47 0.294842127791874 1106 48-49 0.23779016478753334 1106 50-51 0.2863915514509401 1106 52-53 0.2425819346742557 1106 54-55 0.26174568068951487 1106 56-57 0.2899180396853822 1106 58-59 0.25462388478171505 1106 60-61 0.1559612679596043 1106 62-63 0.23460348214808135 1106 64-65 0.29530704301068056 1106 66-67 0.2640528421109991 1106 68-69 0.06564294855944297 1106 70-71 0.1386246087205265 1106 72-73 0.009038942993186083 1106 74-75 0.00657359621208542 1106 76-77 0.12570035285771652 1106 78-79 0.25070685613835764 1106 80-81 0.20469337149710753 1106 82-83 0.11531018916629066 1106 84-85 0.2551775766607456 1106 86-87 0.49060640691510926 1106 88-89 0.579840872262313 1106 90-91 0.5846532523725472 1106 92-93 0.609574241515908 1106 94-95 0.5046349373400183 1106 96-97 0.4771398403817635 1106 98-99 0.40873311918663546 1106 100-101 0.3259353189478382 1107 1 -0.01419466947946546 1107 2 -0.019780484599287718 1107 3 0.10410821835750994 1107 4 0.043458859681926754 1107 5 0.07321917125747746 1107 6 0.0771298603461048 1107 7 0.04753268587446513 1107 8 0.08956360377124639 1107 9 0.1341241623431415 1107 10-11 0.06226833344966565 1107 12-13 0.10153694941946867 1107 14-15 0.18274837131239963 1107 16-17 0.15136964709026302 1107 18-19 0.10727692060261518 1107 20-21 0.10543529629656945 1107 22-23 0.0641257313305843 1107 24-25 0.1139221247396236 1107 26-27 0.06864001395983621 1107 28-29 0.045510393555424855 1107 30-31 0.08764961398287596 1107 32-33 0.1772073381517245 1107 34-35 0.0916956298693421 1107 36-37 0.1816490223177496 1107 38-39 0.10454517301965183 1107 40-41 0.19207979427824284 1107 42-43 0.23920631811583348 1107 44-45 0.07744948466440604 1107 46-47 0.10686916743806307 1107 48-49 0.2126458370003732 1107 50-51 0.15392993565524904 1107 52-53 0.09981858985916148 1107 54-55 0.1097906575791825 1107 56-57 0.09906554377663213 1107 58-59 0.027996641550082302 1107 60-61 0.06678706066139029 1107 62-63 0.04132226598654398 1107 64-65 -0.0269986782441336 1107 66-67 0.0681830308414817 1107 68-69 -0.015076435306440317 1107 70-71 -0.11264568111391071 1107 72-73 -0.05302740308487586 1107 74-75 0.07810812328650485 1107 76-77 0.024993962963076655 1107 78-79 0.19314223894724236 1107 80-81 0.14225296859128278 1107 82-83 0.07104379553638651 1107 84-85 0.23971945307761544 1107 86-87 0.12063274586702377 1107 88-89 0.19655799377316185 1107 90-91 0.3863621384077369 1107 92-93 0.3334449349449429 1107 94-95 0.08484654965872451 1107 96-97 0.17900184599658786 1107 98-99 0.3006184437478261 1107 100-101 0.030338632741646165 1108 1 0.006664308216542736 1108 2 0.030944473127689776 1108 3 0.08433519761652519 1108 4 0.06221643684730083 1108 5 0.11947072964771621 1108 6 0.07116817049423929 1108 7 0.053972059323633914 1108 8 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-0.04249578926256348 2316 84-85 -0.11927761787461932 2316 86-87 -0.1658311610192591 2316 88-89 -0.21866011950266184 2316 90-91 -0.3251144555961645 2316 92-93 -0.3753176673604415 2316 94-95 -0.040058966529404216 2316 96-97 -0.1645633649944429 2316 98-99 -0.19312692629740624 2316 100-101 0.07207741461170514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 14.0 5 44.0 6 78.0 7 189.0 8 556.0 9 1061.0 10 1901.0 11 3053.0 12 4437.0 13 6239.0 14 8070.0 15 10512.0 16 13435.0 17 18212.0 18 18139.0 19 14044.0 20 13230.0 21 13855.0 22 14956.0 23 16728.0 24 19278.0 25 22808.0 26 27030.0 27 32455.0 28 39396.0 29 48172.0 30 58867.0 31 73169.0 32 91466.0 33 117979.0 34 162921.0 35 240475.0 36 392977.0 37 670238.0 38 631660.0 39 69080.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.353962662575434 19.298589458680755 14.621383509953942 23.726064368789874 2 15.44273161524562 20.25531182024838 40.36339298403566 23.93856358047034 3 19.80616380478181 23.5110486001186 29.58933638389466 27.093451211204933 4 13.259405294562827 16.535759967123386 36.888630474855624 33.31620426345817 5 13.921558194614706 37.00470676035848 34.488868788291 14.58486625673582 6 32.707608877525516 36.061254393991476 17.064124096610755 14.167012631872252 7 28.89480394099292 32.159817022328234 20.592829173933104 18.352549862745743 8 25.7275595766086 35.98634388404693 19.5356808560241 18.75041568332037 9 25.60063176863714 17.564554306969267 20.506016994184286 36.32879693020931 10-11 25.005005702300032 26.490824162630016 27.280762490977484 21.223407644092465 12-13 25.963335155922373 23.502122347765393 27.76955356836564 22.764988927946593 14-15 22.379625265317905 25.315655449643288 25.959419465963435 26.345299819075375 16-17 22.224538425105724 28.384173694913024 26.881138885709216 22.510148994272036 18-19 22.159541995421357 27.332623899969892 28.66146376639106 21.84637033821769 20-21 22.670353150547022 26.39422811020386 28.854005717002096 22.08141302224702 22-23 22.289289687343132 26.38727963533284 28.721267093773257 22.602163583550773 24-25 22.395473984406642 26.511965258167645 28.832468883117716 22.260091874307992 26-27 22.143032419472895 26.647236382844735 28.741761936550063 22.467969261132303 28-29 22.062072808815078 27.313212043649727 28.509767420369077 22.114947727166122 30-31 22.28698130139203 26.75598203704225 28.403239941519043 22.55379672004667 32-33 22.019734368731932 26.74844280652576 28.53351825261084 22.69830457213147 34-35 22.62901197841152 26.86431544544844 28.30735773196652 22.19931484417352 36-37 22.73593738729496 26.47918033716796 28.439031256630997 22.345851018906085 38-39 22.296413704871952 26.867610393529972 28.393735143759564 22.442240757838512 40-41 22.583661099442097 26.775718006022064 28.392197611585914 22.248423282949926 42-43 22.371024357062574 26.789022911557232 28.077427133525102 22.76252559785509 44-45 22.48367056388757 26.898893144454817 28.213614473731653 22.403821817925955 46-47 22.88808007120085 27.150049488790856 27.723699398695878 22.23817104131242 48-49 22.651074431139758 27.194430257627538 27.659788967841102 22.494706343391602 50-51 22.48128022896036 27.355386683907774 27.82531060765439 22.338022479477473 52-53 22.922799091692248 27.101144275942413 27.598301701134265 22.377754931231078 54-55 22.479453867377593 27.141180407611188 27.764757895532743 22.614607829478476 56-57 22.56770124848516 27.316800047046602 27.574103646741637 22.5413950577266 58-59 22.70408006985914 26.980641884222372 27.59479739151941 22.720480654399076 60-61 22.343773876929866 27.195119667124633 28.01726061892678 22.443845837018713 62-63 22.331848941386177 27.215692150109238 28.00263808923365 22.449820819270933 64-65 22.092304417928794 27.388281073330635 27.74831465079368 22.77109985794689 66-67 22.15028987934865 27.266179482426693 27.628408333696523 22.955122304528132 68-69 22.49374964556237 26.988211316939704 27.78677242268709 22.73126661481084 70-71 22.546492218494393 27.039758410062653 27.48488093864986 22.928868432793095 72-73 22.408778951686923 26.953904132749823 27.762202537015945 22.87511437854731 74-75 22.22437307954306 27.503656262973696 27.41073572622239 22.861234931260856 76-77 22.372293072338447 27.043202969782108 27.548188008789086 23.03631594909035 78-79 22.4196232480868 27.12137356855766 27.58420980042244 22.8747933829331 80-81 22.57925564351431 27.130315944385053 27.402803711490627 22.88762470061002 82-83 22.51442781524205 27.153119406284443 27.570328434185832 22.762124344287674 84-85 22.361662341226474 27.320335543076695 27.45282913192721 22.86517298376962 86-87 22.49282801588218 27.249684919796252 27.294732671990513 22.962754392331053 88-89 22.326401611104814 27.275435132734266 27.09701511553017 23.301148140630755 90-91 22.346300394555467 27.140250368330836 27.23432518363829 23.2791240534754 92-93 22.5263779508265 26.98927659550635 27.37543327328824 23.10891218037891 94-95 22.217477112038818 26.873550359115384 27.572843469938835 23.336129058906966 96-97 22.225930798090975 26.563511860714055 27.783835572130773 23.426721769064198 98-99 22.108826153786374 26.859582532730915 27.267395315508825 23.76419599797389 100-101 22.27010546195065 26.130567688264588 27.89264251715474 23.706684332630022 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 158.0 1 114.5 2 118.0 3 160.0 4 129.5 5 133.0 6 250.0 7 527.5 8 899.5 9 1116.0 10 1079.0 11 1061.0 12 1218.0 13 1391.5 14 1494.0 15 1633.0 16 1827.0 17 2064.5 18 2334.5 19 2736.5 20 3285.0 21 4021.5 22 5248.5 23 6763.5 24 8734.5 25 11483.0 26 15111.5 27 19498.0 28 23761.5 29 28196.5 30 33236.5 31 39671.5 32 48479.5 33 53658.0 34 55450.0 35 59715.0 36 65652.5 37 71482.5 38 76403.0 39 80619.0 40 85325.0 41 90223.5 42 92876.5 43 95390.5 44 99558.5 45 105393.5 46 108607.5 47 113350.5 48 121745.0 49 118993.0 50 109789.0 51 105102.0 52 102675.5 53 101508.5 54 98899.5 55 91336.5 56 82515.5 57 73627.0 58 65923.0 59 57457.5 60 46606.5 61 38840.0 62 32550.0 63 23953.5 64 16179.5 65 11879.5 66 8972.5 67 6971.5 68 5264.0 69 4002.0 70 3085.0 71 2084.0 72 1416.5 73 1085.0 74 816.5 75 637.0 76 464.5 77 294.0 78 190.5 79 123.0 80 74.0 81 46.5 82 28.5 83 13.5 84 6.5 85 5.0 86 6.5 87 6.5 88 3.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3388125353987164 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.626350576985951E-4 16-17 0.0019252701153971903 18-19 7.000982237807964E-5 20-21 0.0 22-23 0.0 24-25 4.550638454575177E-4 26-27 0.0011551620692383142 28-29 0.0 30-31 0.0029929199066629046 32-33 0.0 34-35 0.010904029835385905 36-37 0.029264105754037293 38-39 0.02166804002601565 40-41 0.014404520954289889 42-43 0.010553980723495506 44-45 0.0031854469182026235 46-47 0.001207669436021874 48-49 7.351031349698363E-4 50-51 1.0501473356711946E-4 52-53 3.500491118903982E-5 54-55 0.0 56-57 0.0 58-59 0.004778170377303936 60-61 0.010413961078739346 62-63 0.004515633543386137 64-65 0.0022403143160985486 66-67 0.004288101620657378 68-69 0.0036405107636601416 70-71 1.750245559451991E-5 72-73 0.0 74-75 0.0 76-77 0.2157177652024579 78-79 2.8003928951231857E-4 80-81 0.0035004911189039823 82-83 7.176006793753164E-4 84-85 0.0 86-87 0.10863774187518509 88-89 0.402188927106473 90-91 0.3897621836343639 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0013651915363725531 100-101 8.576203241314756E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2856742.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.85346405249191 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69045762743461 42.46411247785524 2 14.57402911068707 16.571680802888363 3 4.864650810738296 8.29716749988706 4 2.0867933636238782 4.745657259350754 5 1.1023116144015193 3.133511687201055 6 0.646963408325392 2.2069266527103193 7 0.4274832675890238 1.7012733210840043 8 0.2851144814323264 1.2967796736766115 9 0.21233464614946992 1.086476415476173 >10 1.0215921659570617 10.151763920616444 >50 0.05737121802072907 2.214417714367673 >100 0.02634340814361004 2.769939299189607 >500 0.0021543339514013423 0.872837079520964 >1k 0.002277438748404829 2.096775691201078 >5k 1.2310479721107186E-4 0.3906805049746956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5739 0.20089318531389955 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5425 0.18990164320054104 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4346 0.15213134402756706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2858 0.1000440361782758 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 3.500491118903982E-5 4 0.0 0.0 0.0 0.0 3.500491118903982E-5 5 0.0 0.0 0.0 0.0 3.500491118903982E-5 6 0.0 0.0 0.0 3.500491118903982E-5 3.500491118903982E-5 7 0.0 0.0 0.0 3.500491118903982E-5 3.500491118903982E-5 8 0.0 0.0 0.0 3.500491118903982E-5 7.000982237807964E-5 9 0.0 0.0 0.0 7.000982237807964E-5 7.000982237807964E-5 10-11 0.0 0.0 0.0 7.000982237807964E-5 1.0501473356711946E-4 12-13 0.0 0.0 0.0 7.000982237807964E-5 1.575221003506792E-4 14-15 0.0 0.0 0.0 7.000982237807964E-5 1.92527011539719E-4 16-17 0.0 0.0 0.0 1.4001964475615929E-4 2.1002946713423893E-4 18-19 0.0 0.0 0.0 1.750245559451991E-4 2.1002946713423893E-4 20-21 0.0 0.0 0.0 1.750245559451991E-4 2.450343783232788E-4 22-23 0.0 0.0 0.0 3.500491118903982E-4 2.450343783232788E-4 24-25 0.0 0.0 0.0 6.125859458081969E-4 2.8003928951231857E-4 26-27 0.0 0.0 0.0 7.351031349698363E-4 3.6755156748491814E-4 28-29 0.0 0.0 0.0 0.0020302848489643097 3.8505402307943806E-4 30-31 0.0 0.0 0.0 0.006370893836405248 3.8505402307943806E-4 32-33 0.0 0.0 0.0 0.015139624089259724 4.02556478673958E-4 34-35 0.0 0.0 0.0 0.02949163767676605 4.550638454575177E-4 36-37 0.0 0.0 0.0 0.052804908528666566 4.550638454575177E-4 38-39 0.0 0.0 0.0 0.09390067426459933 4.550638454575177E-4 40-41 0.0 0.0 0.0 0.13861944830859768 4.550638454575177E-4 42-43 0.0 1.750245559451991E-5 0.0 0.1845983991554015 4.550638454575177E-4 44-45 0.0 3.500491118903982E-5 0.0 0.23978364164492277 4.7256630105203763E-4 46-47 0.0 3.500491118903982E-5 0.0 0.30895334615446546 4.900687566465576E-4 48-49 0.0 3.500491118903982E-5 0.0 0.3836888315430655 5.250736678355973E-4 50-51 0.0 3.500491118903982E-5 0.0 0.47284634034154993 5.250736678355973E-4 52-53 0.0 3.500491118903982E-5 0.0 0.5580657966312674 6.650933125917566E-4 54-55 0.0 3.500491118903982E-5 0.0 0.6508463137378173 7.000982237807964E-4 56-57 0.0 3.500491118903982E-5 0.0 0.7642622259903065 7.176006793753163E-4 58-59 0.0 3.500491118903982E-5 0.0 0.8911375265949812 7.351031349698363E-4 60-61 0.0 3.500491118903982E-5 0.0 1.0339400617906693 7.351031349698363E-4 62-63 0.0 3.500491118903982E-5 0.0 1.18047062002799 8.05112957347916E-4 64-65 0.0 3.500491118903982E-5 0.0 1.3324794468663954 8.401178685369557E-4 66-67 0.0 3.500491118903982E-5 0.0 1.507153953699704 8.751227797259956E-4 68-69 0.0 3.500491118903982E-5 0.0 1.716448317698973 9.101276909150354E-4 70-71 0.0 3.500491118903982E-5 0.0 1.946203052288236 9.276301465095553E-4 72-73 0.0 3.500491118903982E-5 0.0 2.186441757778616 9.626350576985951E-4 74-75 0.0 3.500491118903982E-5 0.0 2.4307410329669255 0.0010326448800766748 76-77 0.0 3.500491118903982E-5 0.0 2.6956932057567675 0.0011376596136437943 78-79 0.0 3.500491118903982E-5 0.0 3.007026885872088 0.0011551620692383142 80-81 0.0 3.500491118903982E-5 0.0 3.35984138574642 0.001207669436021874 82-83 0.0 3.500491118903982E-5 0.0 3.717556573187218 0.0012951817139944734 84-85 0.0 3.500491118903982E-5 0.0 4.084565564548706 0.0012951817139944734 86-87 0.0 3.500491118903982E-5 0.0 4.469094513960309 0.0013476890807780331 88-89 1.750245559451991E-5 3.500491118903982E-5 0.0 4.884777834330157 0.0014001964475615929 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 175 5.0240487E-9 27.114578 5 GTATCAA 13685 0.0 20.876738 1 ATACCGA 185 6.6171124E-6 20.51914 6 GTACATG 7795 0.0 20.158304 1 TACATGG 7485 0.0 19.652182 2 GAGTACT 7950 0.0 19.189777 12-13 AGAGTAC 11190 0.0 19.146252 10-11 ACATGGG 7310 0.0 18.56477 3 GTACTTT 8140 0.0 18.508678 14-15 TAAGGTG 1495 0.0 18.408894 5 GTAAGGT 1370 0.0 18.356766 4 GGTATCA 10955 0.0 18.342405 1 TCAACGC 15405 0.0 18.173193 4 CAACGCA 15475 0.0 18.090986 5 ATCAACG 15515 0.0 18.044344 3 TATCAAC 15695 0.0 17.958332 2 AGTACTT 8350 0.0 17.815876 12-13 GTCCGTA 160 0.001199963 17.793941 2 AACGCAG 15870 0.0 17.670607 6 AGGCGAC 1100 0.0 17.254732 5 >>END_MODULE