FastQCFastQC Report
Fri 27 May 2016
SRR939140_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939140_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814061
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.18251203509803093No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49130.1745875444775362No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.1403665378966554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125500.023.0778241
TACATGG71300.020.5240782
GTACATG76500.020.1090851
GTATAGC11950.019.8660181
AGAGTAC104350.019.84647410-11
TCAACGC144200.019.8365614
CAACGCA144000.019.7979835
ATCAACG144550.019.755773
TATCAAC145150.019.7393592
TAAGGTG15650.019.6691685
GTGTAGC19400.019.579291
AACGCAG148150.019.3626526
GAGTACT70400.019.09723312-13
ACATGGG71950.018.8247813
TAGCCCT21300.018.6764534
AGTACTT75800.018.64231912-13
GTAAGGT14800.018.2392864
GTATAGA10850.017.941671
GTACTTT75150.017.73361814-15
ACGCAGA161750.017.6468377
Produced by FastQC (version 0.11.5)