##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939140_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2814061 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.846711567375404 33.0 31.0 34.0 30.0 34.0 2 31.96077021784531 34.0 31.0 34.0 30.0 34.0 3 32.02177742415676 34.0 31.0 34.0 30.0 34.0 4 35.46754921090908 37.0 35.0 37.0 33.0 37.0 5 35.49908264248714 37.0 35.0 37.0 33.0 37.0 6 35.5691319413474 37.0 35.0 37.0 33.0 37.0 7 35.48629080890571 37.0 35.0 37.0 33.0 37.0 8 35.47100969026613 37.0 35.0 37.0 33.0 37.0 9 37.11364465802269 39.0 37.0 39.0 34.0 39.0 10-11 37.00518147971917 39.0 37.0 39.0 33.0 39.0 12-13 36.89697256029631 39.0 37.0 39.0 33.0 39.0 14-15 38.1436759899661 40.0 38.0 41.0 33.0 41.0 16-17 38.042725441985795 40.0 38.0 41.0 33.0 41.0 18-19 37.91302853776091 40.0 38.0 41.0 33.0 41.0 20-21 37.880264855665885 40.0 38.0 41.0 32.5 41.0 22-23 37.923742591223146 40.0 38.0 41.0 33.0 41.0 24-25 37.86932053000983 40.0 38.0 41.0 32.5 41.0 26-27 37.7450103249361 40.0 38.0 41.0 32.0 41.0 28-29 37.592584346963335 40.0 38.0 41.0 32.0 41.0 30-31 37.38972413888683 40.0 37.5 41.0 31.5 41.0 32-33 37.186566673572464 40.0 37.0 41.0 31.0 41.0 34-35 36.87128193027799 40.0 37.0 41.0 30.0 41.0 36-37 36.6033891944773 40.0 36.0 41.0 30.0 41.0 38-39 36.405485524301 40.0 36.0 41.0 30.0 41.0 40-41 36.21261586013949 39.5 36.0 41.0 29.5 41.0 42-43 36.00871924951165 39.0 35.0 41.0 28.5 41.0 44-45 35.75875700633355 39.0 35.0 41.0 27.0 41.0 46-47 35.66415013747037 39.0 35.0 41.0 27.0 41.0 48-49 35.56182168758957 39.0 35.0 41.0 26.0 41.0 50-51 34.46150740868801 38.0 34.0 40.0 24.5 40.5 52-53 34.61955124640155 38.5 34.5 39.5 24.5 40.5 54-55 35.150282278884504 39.0 35.0 40.5 26.0 41.0 56-57 35.05694101869149 39.0 35.0 41.0 25.0 41.0 58-59 34.84747683152568 39.0 35.0 41.0 25.0 41.0 60-61 34.5385032520617 38.0 34.0 40.0 23.5 41.0 62-63 34.153932697265624 38.0 34.0 40.0 22.5 41.0 64-65 33.77490999662054 37.0 34.0 40.0 22.0 41.0 66-67 33.3808057110347 36.5 33.0 39.5 21.0 41.0 68-69 32.95722445249055 36.0 33.0 39.0 19.5 41.0 70-71 32.51160653589244 35.5 32.5 39.0 18.0 40.5 72-73 32.05876809351325 35.0 32.0 38.0 15.5 40.0 74-75 31.605289650792926 35.0 31.5 37.0 12.0 39.5 76-77 31.153529187888964 35.0 31.0 37.0 7.5 39.0 78-79 30.7052784214699 35.0 31.0 36.0 7.0 39.0 80-81 30.237500537479463 34.5 30.5 36.0 2.0 37.5 82-83 29.820038726950127 34.0 30.0 35.5 2.0 37.0 84-85 29.422853307017867 34.0 29.5 35.0 2.0 37.0 86-87 28.996309070769968 34.0 29.0 35.0 2.0 36.0 88-89 28.54096393077478 34.0 29.0 35.0 2.0 36.0 90-91 28.10268967161693 34.0 28.5 35.0 2.0 35.5 92-93 27.660299119315468 34.0 27.0 35.0 2.0 35.0 94-95 27.13929673166289 34.0 26.0 35.0 2.0 35.0 96-97 26.492955909626694 33.0 25.0 35.0 2.0 35.0 98-99 25.718657129323066 33.0 22.0 35.0 2.0 35.0 100-101 22.804108724011314 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27286885662319804 1101 2 0.30879639842276063 1101 3 0.31101649871297354 1101 4 0.3359237285262964 1101 5 0.24790202739530542 1101 6 0.2557209983130164 1101 7 0.20535950859670038 1101 8 0.384495749885275 1101 9 0.5082313524637669 1101 10-11 0.3890938388553522 1101 12-13 0.48375341563036756 1101 14-15 0.5560916266468112 1101 16-17 0.6812610094155218 1101 18-19 0.7645271973600103 1101 20-21 0.7036055446261216 1101 22-23 0.6868534563707271 1101 24-25 0.7550071718720517 1101 26-27 0.6413302613893421 1101 28-29 0.6431681962526099 1101 30-31 0.6633656729315902 1101 32-33 0.5506842265827032 1101 34-35 0.48794523055737216 1101 36-37 0.5581175601727253 1101 38-39 0.35882460011502104 1101 40-41 0.47458366882070635 1101 42-43 0.3701376776554426 1101 44-45 0.2696674825725154 1101 46-47 0.4479364034021671 1101 48-49 0.41169799646349503 1101 50-51 0.3399568079175239 1101 52-53 0.31851338065335 1101 54-55 0.42639845788259834 1101 56-57 0.36168751484799344 1101 58-59 0.2628407461217819 1101 60-61 0.11300600045816367 1101 62-63 0.09906223276704651 1101 64-65 0.07634747685387566 1101 66-67 0.11060257230967352 1101 68-69 0.0934651964008566 1101 70-71 -0.017980336739611857 1101 72-73 0.009790959039122527 1101 74-75 0.009269689490203348 1101 76-77 -0.07972351555175194 1101 78-79 -0.07908964821749365 1101 80-81 -0.27538649163163953 1101 82-83 -0.2986472597816707 1101 84-85 -0.24550703398656992 1101 86-87 -0.34574094374142916 1101 88-89 -0.25556037868518544 1101 90-91 -0.24518462407403518 1101 92-93 -0.1871868961667893 1101 94-95 -0.44006287565102653 1101 96-97 -0.4845548141677938 1101 98-99 -0.491710291688765 1101 100-101 -0.7656024175399061 1102 1 0.3025400685133519 1102 2 0.3793849636711748 1102 3 0.39878383515591764 1102 4 0.28916086368577965 1102 5 0.4123483182089771 1102 6 0.438099098504253 1102 7 0.39851502956204854 1102 8 0.4531626304035683 1102 9 0.48126255902515425 1102 10-11 0.4933817392355522 1102 12-13 0.5457149083147073 1102 14-15 0.5931183703701848 1102 16-17 0.5260318113065381 1102 18-19 0.4803324977251364 1102 20-21 0.6099448430174945 1102 22-23 0.6861416109188454 1102 24-25 0.7844516484526665 1102 26-27 0.7186722434433364 1102 28-29 0.7752086913366512 1102 30-31 0.7496002891993925 1102 32-33 0.8452822819352264 1102 34-35 0.7708450761284524 1102 36-37 0.688016296169387 1102 38-39 0.6211492899863487 1102 40-41 0.6531507402278649 1102 42-43 0.5902714576231531 1102 44-45 0.5748655670228828 1102 46-47 0.7795733850091793 1102 48-49 0.7826076897266461 1102 50-51 0.5055639449525913 1102 52-53 0.6416091809229485 1102 54-55 0.6120047535342295 1102 56-57 0.6649398124383055 1102 58-59 0.6213735136247465 1102 60-61 0.5552767541449057 1102 62-63 0.41178894616555084 1102 64-65 0.439399525184335 1102 66-67 0.540106646884297 1102 68-69 0.5861522596045248 1102 70-71 0.5040274495630186 1102 72-73 0.4084377339988521 1102 74-75 0.4960009849227198 1102 76-77 0.38431672010572626 1102 78-79 0.2914215272828251 1102 80-81 0.271782603653282 1102 82-83 0.13670574439544936 1102 84-85 -5.701465093359559E-4 1102 86-87 -0.034709092119403095 1102 88-89 0.04788482358864954 1102 90-91 0.06241823943429026 1102 92-93 -0.18076863043335933 1102 94-95 -0.2758324522773954 1102 96-97 -0.3924111444388423 1102 98-99 -0.5068024319403719 1102 100-101 -1.024078654592678 1103 1 0.1937645017002012 1103 2 0.252090423506683 1103 3 0.28177673566079164 1103 4 0.20163723663127087 1103 5 0.37284568649217675 1103 6 0.3461232369594356 1103 7 0.2590811857440656 1103 8 0.3550808767157747 1103 9 0.311037328036079 1103 10-11 0.33084641136803583 1103 12-13 0.46416638193012716 1103 14-15 0.4703172859087701 1103 16-17 0.44772550506212383 1103 18-19 0.49535436643729014 1103 20-21 0.45089056069169686 1103 22-23 0.48310575447515447 1103 24-25 0.4523130146438916 1103 26-27 0.5492968564577296 1103 28-29 0.47670021221906467 1103 30-31 0.4622000681506435 1103 32-33 0.47259210829945175 1103 34-35 0.4759910292788234 1103 36-37 0.4143896281057735 1103 38-39 0.5416905932121594 1103 40-41 0.5455180315390393 1103 42-43 0.5172850923980477 1103 44-45 0.4667222305607339 1103 46-47 0.5142040987630665 1103 48-49 0.46084228823632856 1103 50-51 0.3537949108429004 1103 52-53 0.4391635607197415 1103 54-55 0.3729486849558654 1103 56-57 0.4051224224109191 1103 58-59 0.5173771170457613 1103 60-61 0.5196662636897642 1103 62-63 0.3153721859639447 1103 64-65 0.26898656592205583 1103 66-67 0.17717696941451777 1103 68-69 0.20218241635677714 1103 70-71 0.22040601144078664 1103 72-73 0.24608790158884375 1103 74-75 0.16822140848785594 1103 76-77 0.17937979285515837 1103 78-79 0.16319238199608677 1103 80-81 0.10609720223166619 1103 82-83 0.11615044313278844 1103 84-85 0.08466948184707945 1103 86-87 0.02808884922452748 1103 88-89 0.08729668275980984 1103 90-91 0.12156011683725154 1103 92-93 0.04276644924810924 1103 94-95 -0.07455133133521485 1103 96-97 -0.011323780372233472 1103 98-99 -0.10369770172032844 1103 100-101 -0.2694937822625043 1104 1 0.09789668236869886 1104 2 0.2682595977435547 1104 3 0.25604515537778383 1104 4 0.27211884178260703 1104 5 0.25714935521911286 1104 6 0.23502139105640651 1104 7 0.18158047416514478 1104 8 0.22643479391260968 1104 9 0.21895047962323844 1104 10-11 0.3954146889662198 1104 12-13 0.3764363334705365 1104 14-15 0.45227010141400115 1104 16-17 0.36542003531005207 1104 18-19 0.49676545945206385 1104 20-21 0.4387072953865072 1104 22-23 0.5260270133017713 1104 24-25 0.5769704800834745 1104 26-27 0.49288719644964374 1104 28-29 0.5530271879052222 1104 30-31 0.5624066312227995 1104 32-33 0.4808282034563405 1104 34-35 0.5928809582785135 1104 36-37 0.568257121112282 1104 38-39 0.39738394685162604 1104 40-41 0.6139368785480954 1104 42-43 0.512943558151818 1104 44-45 0.5709360417249272 1104 46-47 0.8017810469545381 1104 48-49 0.6755036538178345 1104 50-51 0.6037393469523025 1104 52-53 0.5771845932527668 1104 54-55 0.582496366981367 1104 56-57 0.6115913560405488 1104 58-59 0.5888294898454873 1104 60-61 0.6058867307022169 1104 62-63 0.46863922782777223 1104 64-65 0.573805801894764 1104 66-67 0.5964402562427153 1104 68-69 0.5777246362138229 1104 70-71 0.47721189041229195 1104 72-73 0.38045887074456886 1104 74-75 0.33718914794524224 1104 76-77 0.2796192674784628 1104 78-79 0.28472669717154275 1104 80-81 0.18718880205478072 1104 82-83 0.030996523769211137 1104 84-85 -0.04176963933407407 1104 86-87 -0.05128642551715856 1104 88-89 0.11334367224748476 1104 90-91 0.1339177887414209 1104 92-93 -0.009303153840228617 1104 94-95 0.10992948525207424 1104 96-97 0.10372031125746517 1104 98-99 0.1813646726453655 1104 100-101 -4.814410178255457E-5 1105 1 0.2351610415451546 1105 2 0.35130652251742234 1105 3 0.3388181061918729 1105 4 0.33600059152581707 1105 5 0.33089354836425144 1105 6 0.38336699078695347 1105 7 0.30093343744891854 1105 8 0.2646236681551528 1105 9 0.3489936901151083 1105 10-11 0.4502272814904771 1105 12-13 0.4038603529068965 1105 14-15 0.5931088146465413 1105 16-17 0.47726595730827626 1105 18-19 0.47518018110999805 1105 20-21 0.48816702492931796 1105 22-23 0.4452930788561531 1105 24-25 0.5219202886731935 1105 26-27 0.5882143461236282 1105 28-29 0.40839912071256634 1105 30-31 0.5500474646732556 1105 32-33 0.5778883545166096 1105 34-35 0.5186889647573025 1105 36-37 0.6308055309178897 1105 38-39 0.6633974926195165 1105 40-41 0.5462538562035846 1105 42-43 0.524273232720283 1105 44-45 0.47298802928991535 1105 46-47 0.5468064847401592 1105 48-49 0.5681677766027562 1105 50-51 0.5649613513024576 1105 52-53 0.579453004767224 1105 54-55 0.742051835718307 1105 56-57 0.7619439311171519 1105 58-59 0.6973293694207783 1105 60-61 0.7729611593658987 1105 62-63 0.7058884487820407 1105 64-65 0.7473026754958738 1105 66-67 0.7183874588292696 1105 68-69 0.6790611028598903 1105 70-71 0.5435281387088509 1105 72-73 0.5543027780280667 1105 74-75 0.5130115496659862 1105 76-77 0.5011611124345876 1105 78-79 0.4715117721846873 1105 80-81 0.4982936631886794 1105 82-83 0.423421345005071 1105 84-85 0.14224953698565912 1105 86-87 0.18320432654600083 1105 88-89 0.17866850629975417 1105 90-91 0.21068266088381193 1105 92-93 0.1474005091142203 1105 94-95 0.07631969103902492 1105 96-97 0.14701949639389866 1105 98-99 0.29883836031009636 1105 100-101 0.038377555089049054 1106 1 0.18844981638552127 1106 2 0.2114589961105473 1106 3 0.29353732800962007 1106 4 0.30114978290851724 1106 5 0.280122527298424 1106 6 0.12576330277597236 1106 7 0.18747279413567952 1106 8 0.2142787376783417 1106 9 0.28628825358112664 1106 10-11 0.20232625505376234 1106 12-13 0.18165918324645958 1106 14-15 0.294287874144068 1106 16-17 0.22807412702839258 1106 18-19 0.20134984155041025 1106 20-21 0.26553782519189895 1106 22-23 0.2437865442735898 1106 24-25 0.30939343422430454 1106 26-27 0.35087233979791677 1106 28-29 0.20582094442803367 1106 30-31 0.17831997765290453 1106 32-33 0.23471057806497697 1106 34-35 0.24257062615253489 1106 36-37 0.1658077687886248 1106 38-39 0.1622963110237592 1106 40-41 0.3975172088323333 1106 42-43 0.35670714093775047 1106 44-45 0.21923765631146352 1106 46-47 0.23932647638543614 1106 48-49 0.31409917840498025 1106 50-51 0.24046397707078881 1106 52-53 0.2067396610899621 1106 54-55 0.24001984908586138 1106 56-57 0.26624541706332394 1106 58-59 0.3204159899375725 1106 60-61 0.4473102373631477 1106 62-63 0.3452528934917112 1106 64-65 0.3592056115822828 1106 66-67 0.2828916958645422 1106 68-69 0.28850491738516126 1106 70-71 0.1992233705522608 1106 72-73 0.2818314227735428 1106 74-75 0.26861219417275706 1106 76-77 0.19768913057626136 1106 78-79 0.11393076126387669 1106 80-81 0.3228814473982631 1106 82-83 0.3679254328489563 1106 84-85 0.47671185124238846 1106 86-87 0.5443578743170825 1106 88-89 0.5639595786874132 1106 90-91 0.575112153036347 1106 92-93 0.4521782139539887 1106 94-95 0.5035420458758075 1106 96-97 0.590952032050641 1106 98-99 0.36919925426486344 1106 100-101 0.11319852542980158 1107 1 0.07009091805627321 1107 2 0.09501519158297711 1107 3 0.1551196967157864 1107 4 0.11935101459010866 1107 5 0.08083359532602685 1107 6 0.1781248811531171 1107 7 0.12073732056551023 1107 8 0.20118242702087485 1107 9 0.10578161048367463 1107 10-11 0.07541900181774253 1107 12-13 0.09580784037139978 1107 14-15 0.1727615298434486 1107 16-17 0.15615518422175256 1107 18-19 0.1579744067554003 1107 20-21 0.10420735247706858 1107 22-23 0.1973815149071072 1107 24-25 0.20671409910427485 1107 26-27 0.09494145082032901 1107 28-29 0.29607027441605993 1107 30-31 0.31044277516673446 1107 32-33 0.2442663743813398 1107 34-35 0.3557749940603827 1107 36-37 0.3969571957145561 1107 38-39 0.33385088136076746 1107 40-41 0.43937613567272393 1107 42-43 0.44723602273052876 1107 44-45 0.39604355241684175 1107 46-47 0.43507788768726385 1107 48-49 0.38081683250774034 1107 50-51 0.34258820014200353 1107 52-53 0.40913217406252755 1107 54-55 0.2759324909288168 1107 56-57 0.3012839071129392 1107 58-59 0.4560399564084605 1107 60-61 0.324967904010812 1107 62-63 0.43738495712859304 1107 64-65 0.42198958261495534 1107 66-67 0.33674654561079365 1107 68-69 0.26217772345233215 1107 70-71 0.3572101984019511 1107 72-73 0.41441695621711716 1107 74-75 0.36216582364187744 1107 76-77 0.32002932559251107 1107 78-79 0.27555390091147913 1107 80-81 0.2873527081612224 1107 82-83 0.3434340717267581 1107 84-85 0.4498552052938223 1107 86-87 0.347112051200245 1107 88-89 0.37461861387017237 1107 90-91 0.2536606504988512 1107 92-93 0.18619429424137834 1107 94-95 0.1092291645392045 1107 96-97 0.2951587093614698 1107 98-99 0.1144357542363501 1107 100-101 0.06722287395007243 1108 1 0.14037854941719274 1108 2 0.2229691771060267 1108 3 0.2629547488241002 1108 4 0.2833782896958539 1108 5 0.25675148657444424 1108 6 0.2853843434999561 1108 7 0.20859270363794025 1108 8 0.2774687829272082 1108 9 0.318765629146732 1108 10-11 0.31556720528000426 1108 12-13 0.3386586176355948 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5 4089.0 6 4604.0 7 5344.0 8 6289.0 9 7796.0 10 8991.0 11 10692.0 12 12922.0 13 18523.0 14 16103.0 15 15621.0 16 17807.0 17 22063.0 18 19494.0 19 16734.0 20 17138.0 21 17865.0 22 19488.0 23 21994.0 24 24397.0 25 27815.0 26 31162.0 27 35815.0 28 42187.0 29 48920.0 30 58989.0 31 72045.0 32 89804.0 33 115673.0 34 158820.0 35 233543.0 36 372263.0 37 579868.0 38 544879.0 39 93575.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07479254022376 18.736662535664674 14.25067262558353 23.93787229852804 2 15.085589499142932 19.67754422141911 41.00842411007485 24.228442169363106 3 19.755356839171366 23.28496826064341 29.28513334372218 27.67454155646304 4 12.87621621775493 15.973799723308682 37.120616919161726 34.02936713977466 5 14.031934543899233 36.8619700808914 34.45764543497143 14.648449940237931 6 32.90823819931593 35.47360275505175 17.293717847594486 14.324441198037837 7 28.9961098572334 31.949922503007055 20.645106450779984 18.408861188979564 8 25.82743113409358 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22.691900697691374 32-33 22.007883096976176 26.662975818423075 28.468266559865636 22.860874524735113 34-35 22.7266033625964 26.79435519095872 28.173288563371283 22.305752883073595 36-37 22.758541850310753 26.38232086830944 28.35193218141044 22.507205099969365 38-39 22.289705723201724 26.771130864257504 28.32069959807542 22.618463814465358 40-41 22.67247692696076 26.659746629516995 28.303362573882467 22.364413869639783 42-43 22.36145330534028 26.69329888253989 28.026707460901758 22.918540351218077 44-45 22.53928320233872 26.796753967434654 28.14889842002744 22.515064410199184 46-47 22.88142010684521 27.069679421509925 27.62022943572642 22.42867103591845 48-49 22.67024271767493 27.062669781898634 27.62356514257616 22.643522357850276 50-51 22.519740057098275 27.285842170991902 27.735601832403127 22.45881593950669 52-53 22.87887645237752 27.084589174501776 27.525966553411003 22.5105678197097 54-55 22.46762053747077 27.015738636089647 27.784569482051836 22.732071344387748 56-57 22.568873843472918 27.238386521973567 27.562106977192624 22.630632657360884 58-59 22.69091702406582 26.89788724033701 27.622141602725204 22.789054132871968 60-61 22.332423671756704 27.078847923783993 28.02219802643764 22.566530378021664 62-63 22.346949817732987 27.039017318848163 28.122565859515653 22.491467003903196 64-65 22.132073574300115 27.285604027199277 27.810795367570528 22.771527030930077 66-67 22.20163737926427 27.15913089923669 27.64611100095213 22.993120720546912 68-69 22.484330384564398 26.852812991210868 27.89254079495628 22.77031582926846 70-71 22.480679845063587 26.904274900833297 27.5784280289056 23.036617225197517 72-73 22.417204347173694 26.81753874515016 27.842757925875116 22.922498981801034 74-75 22.22204358354895 27.366319321630357 27.56076751748694 22.850869577333754 76-77 22.365443682319107 26.970575008742237 27.617804086044316 23.046177222894336 78-79 22.451241684799697 26.971021849445886 27.63902322140818 22.938713244346236 80-81 22.584631097961104 27.01343758900137 27.451911358874064 22.950019954163455 82-83 22.466484938491725 27.012760975303113 27.73497688751926 22.7857771986859 84-85 22.366893766446758 27.191311683376522 27.59567691353827 22.846117636638446 86-87 22.47654888772735 27.1014123436719 27.485083951490765 22.936954817109985 88-89 22.327980131681546 27.08379991234565 27.295917000549714 23.292302955423086 90-91 22.30964527303721 26.995192913141796 27.475097768154832 23.220064045666163 92-93 22.52032251639244 26.82734726069182 27.643445569776283 23.00888465313946 94-95 22.259940816500787 26.66011930301659 27.83584027814744 23.244099602335186 96-97 22.303587202432258 26.33085004809672 28.002025689096023 23.363537060375002 98-99 22.147293500374506 26.587902489808524 27.652931970551947 23.611872039265023 100-101 22.491685220847284 25.76446587623208 28.188864919909694 23.55498398301094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2150.0 1 1162.0 2 205.0 3 238.5 4 224.5 5 233.0 6 388.5 7 728.5 8 1172.0 9 1431.5 10 1489.5 11 1581.0 12 1810.0 13 2099.5 14 2382.0 15 2633.5 16 2868.0 17 3065.0 18 3244.0 19 3526.5 20 3868.0 21 4440.5 22 5505.5 23 6958.0 24 8748.0 25 11231.5 26 14809.0 27 19014.0 28 22968.5 29 27021.5 30 31877.0 31 38304.5 32 46504.5 33 51320.5 34 53660.0 35 58052.5 36 63524.0 37 69391.0 38 74574.0 39 78441.0 40 82710.0 41 87967.0 42 91066.0 43 93518.0 44 97769.0 45 103501.0 46 106431.0 47 110487.0 48 119105.0 49 117578.0 50 107894.5 51 102427.5 52 99904.0 53 98615.5 54 96133.5 55 89281.0 56 80752.5 57 72443.0 58 65202.0 59 57047.0 60 46754.5 61 38972.5 62 32797.0 63 24588.5 64 17105.5 65 12630.5 66 9543.5 67 7431.0 68 5772.5 69 4408.5 70 3354.0 71 2374.5 72 1731.0 73 1334.0 74 976.0 75 740.0 76 558.5 77 408.5 78 277.0 79 193.0 80 139.5 81 94.5 82 70.0 83 47.5 84 37.5 85 28.0 86 20.0 87 18.0 88 12.0 89 6.5 90 4.0 91 4.5 92 5.5 93 5.0 94 2.0 95 2.0 96 3.5 97 3.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3861678904615074 2 0.11794342766556945 3 0.11922271763120985 4 0.13180240229334048 5 0.13325937142087538 6 0.07331042219767091 7 0.08311831193424733 8 0.08016883784679862 9 0.07945812119922063 10-11 0.07762802583170728 12-13 0.08205223696288033 14-15 0.07556694755373107 16-17 0.07750365041838113 18-19 0.086973949747358 20-21 0.0856591239493387 22-23 0.09557362118305184 24-25 0.10836652083945587 26-27 0.10984125788318022 28-29 0.1038001663787672 30-31 0.09402781247456968 32-33 0.09248200376608752 34-35 0.3376614792643088 36-37 0.5269430904305201 38-39 0.5075227580354512 40-41 0.47966266545039354 42-43 0.5088375838334706 44-45 0.5181835077491214 46-47 0.5151629619969148 48-49 0.5222878963888842 50-51 0.5215594118251168 52-53 0.5203334256080447 54-55 0.5147542999245575 56-57 0.4850996478043653 58-59 0.47573595597252516 60-61 0.4235871219564892 62-63 0.49920737325878867 64-65 0.5257704079620165 66-67 0.5357382089442979 68-69 0.5505211152139203 70-71 0.5459902965856106 72-73 0.5330730215158804 74-75 0.537124106407075 76-77 0.512924204557044 78-79 0.4865743848480896 80-81 0.49371708715624857 82-83 0.5072740072087989 84-85 0.5016593456929328 86-87 0.5024944377538368 88-89 0.5127109895627707 90-91 0.5317759636340506 92-93 0.5377815193060848 94-95 0.5319714107121345 96-97 0.5335172194206167 98-99 0.534121328571058 100-101 0.5109164300276362 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2814061.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.883583202071286 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43031577483337 45.004908580879835 2 13.675239711192551 16.104942306845523 3 4.45550820442417 7.870688641881657 4 1.9564931865845188 4.6082131734614045 5 1.0142820418333967 2.9862280500331786 6 0.6076836897652255 2.1469555864099394 7 0.3772816443700191 1.5550986567813405 8 0.2839329864831931 1.3375193306716544 9 0.18765404522289195 0.9944768326578665 >10 0.9338287986107876 9.585692191814562 >50 0.04937591732380918 1.9721077670550262 >100 0.02398932254999156 2.588932448749882 >500 0.002237575915478822 0.9386047296848998 >1k 0.00211662586583916 2.1227394543669513 >5k 6.0475024738261704E-5 0.1828922487062566 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5136 0.18251203509803093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4913 0.1745875444775362 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.1403665378966554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.066074971367003E-4 2 0.0 0.0 0.0 0.0 1.066074971367003E-4 3 0.0 0.0 0.0 0.0 1.066074971367003E-4 4 0.0 0.0 0.0 3.5535832378900104E-5 1.066074971367003E-4 5 0.0 0.0 0.0 3.5535832378900104E-5 1.066074971367003E-4 6 0.0 0.0 0.0 3.5535832378900104E-5 1.4214332951560042E-4 7 0.0 0.0 0.0 3.5535832378900104E-5 1.4214332951560042E-4 8 0.0 0.0 0.0 3.5535832378900104E-5 1.7767916189450052E-4 9 0.0 0.0 0.0 3.5535832378900104E-5 1.7767916189450052E-4 10-11 0.0 0.0 0.0 5.330374856835016E-5 1.7767916189450052E-4 12-13 0.0 0.0 0.0 7.107166475780021E-5 3.55358323789001E-4 14-15 0.0 0.0 0.0 7.107166475780021E-5 4.7973373711515134E-4 16-17 0.0 0.0 0.0 8.883958094725025E-5 5.685733180624017E-4 18-19 0.0 0.0 0.0 1.4214332951560042E-4 6.041091504413017E-4 20-21 0.0 0.0 0.0 1.4214332951560042E-4 7.995562285252522E-4 22-23 0.0 0.0 0.0 2.309829104628507E-4 9.594674742303027E-4 24-25 0.0 0.0 0.0 3.198224914101009E-4 0.001030539138988103 26-27 0.0 0.0 0.0 5.685733180624017E-4 0.0011371466361248033 28-29 0.0 0.0 0.0 0.0020966141103551062 0.0012082183008826034 30-31 0.0 0.0 0.0 0.006787343984369919 0.0012082183008826034 32-33 0.0 0.0 0.0 0.014978353347706395 0.0012082183008826034 34-35 0.0 0.0 0.0 0.02812661132789943 0.0012792899656404037 36-37 0.0 0.0 0.0 0.0521843698484148 0.0012792899656404037 38-39 0.0 0.0 0.0 0.09283736208987652 0.0012792899656404037 40-41 0.0 0.0 0.0 0.138376531283437 0.0012792899656404037 42-43 0.0 0.0 0.0 0.18396900422556584 0.0012792899656404037 44-45 0.0 0.0 0.0 0.24121723018797392 0.0012792899656404037 46-47 0.0 0.0 0.0 0.30830888171933724 0.0012970578818298538 48-49 0.0 0.0 0.0 0.3835382388654688 0.0014214332951560042 50-51 0.0 0.0 0.0 0.4721113010698773 0.0014214332951560042 52-53 0.0 0.0 0.0 0.5598492712133816 0.0014392012113454541 54-55 0.0 0.0 0.0 0.6526688653870687 0.001456969127534904 56-57 0.0 0.0 0.0 0.766419064831928 0.0014747370437243543 58-59 0.0 0.0 0.0 0.8942947576474 0.0014925049599138042 60-61 0.0 0.0 0.0 1.036935588816305 0.0014925049599138042 62-63 0.0 0.0 0.0 1.1810511570289344 0.0014925049599138042 64-65 0.0 0.0 0.0 1.3315454071535764 0.0015280407922927044 66-67 0.0 0.0 0.0 1.5086737636462038 0.0015813445408610544 68-69 0.0 0.0 0.0 1.7168071338894217 0.0015991124570505046 70-71 0.0 0.0 0.0 1.944414140276277 0.0016168803732399548 72-73 0.0 0.0 0.0 2.186981732094649 0.001759023702755555 74-75 0.0 0.0 0.0 2.4296381634939683 0.0018833991160817055 76-77 0.0 0.0 0.0 2.690417869406527 0.0018833991160817055 78-79 0.0 0.0 0.0 3.00466123513314 0.0018833991160817055 80-81 0.0 0.0 0.0 3.3546714161491167 0.0019367028646500555 82-83 0.0 0.0 0.0 3.717581104318634 0.0022387574398707063 84-85 0.0 0.0 0.0 4.082622942430886 0.0022387574398707063 86-87 0.0 0.0 0.0 4.455820254074094 0.0023275970208179566 88-89 0.0 0.0 0.0 4.868497875490261 0.0024164366017652068 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12550 0.0 23.077824 1 TACATGG 7130 0.0 20.524078 2 GTACATG 7650 0.0 20.109085 1 GTATAGC 1195 0.0 19.866018 1 AGAGTAC 10435 0.0 19.846474 10-11 TCAACGC 14420 0.0 19.836561 4 CAACGCA 14400 0.0 19.797983 5 ATCAACG 14455 0.0 19.75577 3 TATCAAC 14515 0.0 19.739359 2 TAAGGTG 1565 0.0 19.669168 5 GTGTAGC 1940 0.0 19.57929 1 AACGCAG 14815 0.0 19.362652 6 GAGTACT 7040 0.0 19.097233 12-13 ACATGGG 7195 0.0 18.824781 3 TAGCCCT 2130 0.0 18.676453 4 AGTACTT 7580 0.0 18.642319 12-13 GTAAGGT 1480 0.0 18.239286 4 GTATAGA 1085 0.0 17.94167 1 GTACTTT 7515 0.0 17.733618 14-15 ACGCAGA 16175 0.0 17.646837 7 >>END_MODULE