FastQCFastQC Report
Fri 27 May 2016
SRR939140_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939140_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814061
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53630.19057866904804124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53050.18851759077006502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42830.15219997007882913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG1658.139614E-825.9995961
GTACATG74750.020.7243161
TACATGG72200.020.6390782
GTATCAA135400.020.0661581
TAAGGTG15700.019.6476925
ACATGGG71400.019.4745433
GAGTACT78300.019.00155312-13
GTAAGCG2450.018.40175494-95
GTACTTT81350.018.20163314-15
GGTATCA108200.018.194111
AGAGTAC111100.018.13330310-11
ATCAACG152100.017.5349663
CAACGCA153050.017.488145
TCAACGC152800.017.4856974
TATCAAC154350.017.2793542
AGTACTT82900.017.1170912-13
AACGCAG157000.017.078386
GTAAGGT14250.016.6514874
GTGTAGC17300.016.5315361
CATGGGG44850.016.1892554
Produced by FastQC (version 0.11.5)