##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939140_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2814061 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47050970110456 34.0 31.0 34.0 31.0 34.0 2 32.689588463078806 34.0 31.0 34.0 31.0 34.0 3 32.80731654360016 34.0 31.0 34.0 31.0 34.0 4 36.26106683543818 37.0 37.0 37.0 35.0 37.0 5 36.2169345298485 37.0 37.0 37.0 35.0 37.0 6 36.250866985470466 37.0 37.0 37.0 35.0 37.0 7 36.20378556115166 37.0 37.0 37.0 35.0 37.0 8 36.16842882936795 37.0 37.0 37.0 35.0 37.0 9 37.92031444947355 39.0 38.0 39.0 35.0 39.0 10-11 37.832424563646626 39.0 38.0 39.0 35.0 39.0 12-13 37.74971704593469 39.0 38.0 39.0 35.0 39.0 14-15 39.17016351102552 40.5 38.5 41.0 36.0 41.0 16-17 39.26943889986749 41.0 39.0 41.0 36.0 41.0 18-19 39.28527206766307 41.0 39.0 41.0 36.0 41.0 20-21 39.241680262083875 41.0 39.0 41.0 36.0 41.0 22-23 39.177545547164755 41.0 39.0 41.0 36.0 41.0 24-25 39.10577080596334 41.0 39.0 41.0 35.5 41.0 26-27 38.96265663750715 40.0 38.5 41.0 35.0 41.0 28-29 38.81396192193417 40.0 38.0 41.0 35.0 41.0 30-31 38.635001160244926 40.0 38.0 41.0 34.5 41.0 32-33 38.55171938348174 40.0 38.0 41.0 34.0 41.0 34-35 38.45795933350414 40.0 38.0 41.0 34.0 41.0 36-37 38.31016349681119 40.0 38.0 41.0 34.0 41.0 38-39 38.180721917541945 40.0 38.0 41.0 34.0 41.0 40-41 38.00367085148474 40.0 38.0 41.0 33.0 41.0 42-43 37.82134502414837 40.0 38.0 41.0 33.0 41.0 44-45 37.59909539985097 40.0 37.0 41.0 33.0 41.0 46-47 37.33716362225268 40.0 37.0 41.0 32.0 41.0 48-49 37.020429372355466 40.0 36.5 41.0 31.0 41.0 50-51 36.762653865712224 39.5 36.0 41.0 31.0 41.0 52-53 36.48810171492374 39.0 36.0 41.0 30.0 41.0 54-55 36.23198821916085 39.0 35.0 40.0 30.0 41.0 56-57 35.96030896274104 39.0 35.0 40.0 29.0 41.0 58-59 35.604994525705024 38.0 35.0 40.0 28.5 41.0 60-61 35.21238345579573 38.0 34.0 40.0 28.0 41.0 62-63 34.797070852408666 37.5 34.0 40.0 27.0 41.0 64-65 34.33105145908351 37.0 34.0 39.5 26.0 41.0 66-67 33.89542621855034 36.0 33.0 39.0 26.0 40.5 68-69 33.38089277382402 36.0 33.0 39.0 25.5 40.0 70-71 32.84726912458543 35.0 32.0 38.0 23.5 40.0 72-73 32.24287089014773 35.0 31.5 37.5 22.0 39.5 74-75 31.680243782917287 35.0 31.0 37.0 20.5 39.0 76-77 29.700068690763988 32.5 28.5 35.0 18.0 37.0 78-79 30.67499940477481 34.0 30.0 36.0 18.0 38.0 80-81 30.678904259715765 34.0 31.0 35.5 18.0 37.0 82-83 30.2659659829691 34.0 30.0 35.0 15.0 37.0 84-85 29.7642631058815 34.0 30.0 35.0 8.5 36.0 86-87 29.0307825594399 34.0 29.0 35.0 3.5 36.0 88-89 28.31205240398129 33.0 28.0 35.0 2.0 35.0 90-91 27.71570605612316 33.0 26.5 35.0 2.0 35.0 92-93 27.22518328493945 33.0 26.0 34.5 2.0 35.0 94-95 26.646056357698 32.0 25.0 34.0 2.0 35.0 96-97 25.796084200022673 32.0 24.0 34.0 2.0 35.0 98-99 24.501350894667883 32.0 10.5 34.0 2.0 35.0 100-101 18.62981044120934 24.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10608352503922447 1101 2 0.16156782761112964 1101 3 0.23224594690213252 1101 4 0.15337510704431878 1101 5 0.17883368871723349 1101 6 0.13569422655849195 1101 7 0.14017110609000127 1101 8 0.19772252727926087 1101 9 0.18614058309835713 1101 10-11 0.23587840541979688 1101 12-13 0.2507491328560505 1101 14-15 0.29677398401302213 1101 16-17 0.2771731625599543 1101 18-19 0.3197525149222571 1101 20-21 0.30554900942386354 1101 22-23 0.30202629197135167 1101 24-25 0.29893052027884437 1101 26-27 0.25866178768293935 1101 28-29 0.32460083291054076 1101 30-31 0.31515922925617446 1101 32-33 0.37464517410349885 1101 34-35 0.36140667191498466 1101 36-37 0.399476312010016 1101 38-39 0.3519208481497955 1101 40-41 0.35724885529530326 1101 42-43 0.2790773624924583 1101 44-45 0.16500112603763029 1101 46-47 0.19450245814035583 1101 48-49 0.29400244232930817 1101 50-51 0.24751966633049705 1101 52-53 0.23652782125117966 1101 54-55 0.2246401989411666 1101 56-57 0.20944308777576026 1101 58-59 0.3028086893331121 1101 60-61 0.32391970919006496 1101 62-63 0.1905541803430495 1101 64-65 0.21937223452314214 1101 66-67 0.2761955385901018 1101 68-69 0.27528033833518606 1101 70-71 0.27261148127649193 1101 72-73 0.36365281139390504 1101 74-75 0.1290442851687068 1101 76-77 0.2062260870130359 1101 78-79 0.22917672813771617 1101 80-81 0.4076667689995439 1101 82-83 0.2497095360832624 1101 84-85 0.33112164367933516 1101 86-87 0.5301895938208965 1101 88-89 0.39722336239026745 1101 90-91 0.47459151455912263 1101 92-93 0.250456888249488 1101 94-95 0.1059940902530947 1101 96-97 -0.0835703238908252 1101 98-99 -0.17109817361798463 1101 100-101 -1.0516494777464942 1102 1 0.10537798996317349 1102 2 0.08754887194326955 1102 3 0.05951395732827791 1102 4 0.09221459387053699 1102 5 0.11901237752631033 1102 6 0.11263891786718006 1102 7 0.12239057044426715 1102 8 0.13511290641014995 1102 9 0.15750479870987277 1102 10-11 0.18675458357572694 1102 12-13 0.21161717763867927 1102 14-15 0.24340108812896233 1102 16-17 0.20646505074711285 1102 18-19 0.20175023968119632 1102 20-21 0.1845038183684693 1102 22-23 0.15275356278639407 1102 24-25 0.21078990757641236 1102 26-27 0.19178232112004423 1102 28-29 0.21784040457625053 1102 30-31 0.21609342781214735 1102 32-33 0.1428790927241934 1102 34-35 0.19687571689219396 1102 36-37 0.22136209771316118 1102 38-39 0.16221140589937733 1102 40-41 0.1688376852289153 1102 42-43 0.14784520902411913 1102 44-45 0.242291923932072 1102 46-47 0.25677973254880726 1102 48-49 0.21083983971996645 1102 50-51 0.21280514948617935 1102 52-53 0.24902955222233203 1102 54-55 0.13654910516022056 1102 56-57 0.17239215427835575 1102 58-59 0.09180751314917046 1102 60-61 0.13332847956074545 1102 62-63 4.2807799927402357E-4 1102 64-65 -0.005727285362425505 1102 66-67 0.1202594907434289 1102 68-69 0.09593717834436433 1102 70-71 0.08265961542105771 1102 72-73 -0.027436587349974673 1102 74-75 -0.06464472548706368 1102 76-77 0.07465711723447654 1102 78-79 0.020962459326224092 1102 80-81 0.25686167021586925 1102 82-83 0.10873083184882759 1102 84-85 0.1527785029702251 1102 86-87 0.3505126850636735 1102 88-89 0.2222190240068791 1102 90-91 0.27245071955693945 1102 92-93 -0.08063450878007572 1102 94-95 -0.1288430406994685 1102 96-97 0.02887417547726301 1102 98-99 -0.14906321764161845 1102 100-101 -0.5744591200007712 1103 1 0.06454322605337381 1103 2 0.014097823421607814 1103 3 0.044416859902803196 1103 4 0.042863699155525126 1103 5 0.09949533556054035 1103 6 0.07906492707839874 1103 7 0.08082121017172028 1103 8 0.059237191801777556 1103 9 0.1289766278754314 1103 10-11 0.12734674347281327 1103 12-13 0.03259482457728069 1103 14-15 -0.11863202796285321 1103 16-17 0.036659589108175794 1103 18-19 0.09285453020829948 1103 20-21 0.09497538045491183 1103 22-23 0.11505350035343298 1103 24-25 0.04385215418403021 1103 26-27 0.06883361319037107 1103 28-29 0.08878872433950136 1103 30-31 0.07591399530802079 1103 32-33 0.07027823171610947 1103 34-35 0.13283564300316897 1103 36-37 0.08144974431683494 1103 38-39 0.13463604622283043 1103 40-41 0.21110789199624236 1103 42-43 0.24214259774616664 1103 44-45 0.19763921694362097 1103 46-47 0.18187864199008885 1103 48-49 0.21908717386684629 1103 50-51 0.27675986111373874 1103 52-53 0.1837050081675855 1103 54-55 0.03401176775857806 1103 56-57 0.06842444079326526 1103 58-59 -0.07524222453792362 1103 60-61 0.17947665779783506 1103 62-63 -0.01381720023962174 1103 64-65 0.09773649879642932 1103 66-67 0.10412722561201804 1103 68-69 0.061572255535388365 1103 70-71 -0.09525869615806926 1103 72-73 -0.023534999560389736 1103 74-75 -0.044764335706144465 1103 76-77 -0.05277705978830127 1103 78-79 -0.022523601463042553 1103 80-81 0.12390470945588916 1103 82-83 -0.0649459028128021 1103 84-85 -0.18004689341897162 1103 86-87 -0.3198715911047145 1103 88-89 -0.1604969214746319 1103 90-91 -0.11540731458624975 1103 92-93 -0.08057911777540383 1103 94-95 -0.2047141179214016 1103 96-97 -0.3768834832952592 1103 98-99 -0.7896972857716982 1103 100-101 -1.0327849891457461 1104 1 0.09237704192531027 1104 2 0.078746615313257 1104 3 0.08914488902123452 1104 4 0.05350865722078879 1104 5 0.022294521258181987 1104 6 0.029243076237818855 1104 7 0.07483672472618252 1104 8 0.09588063380177658 1104 9 0.07476925128882073 1104 10-11 0.13791988705614244 1104 12-13 0.08869473846556275 1104 14-15 0.055409178940657 1104 16-17 0.06186236858305705 1104 18-19 0.14859972159266732 1104 20-21 0.20753805603106912 1104 22-23 0.15395043975973266 1104 24-25 0.1539247138951083 1104 26-27 0.16097733867290742 1104 28-29 0.08840732788515027 1104 30-31 0.04531265448650146 1104 32-33 0.11367486137628902 1104 34-35 0.03402613834417423 1104 36-37 0.16028546659652676 1104 38-39 0.16231712295090261 1104 40-41 0.18006335322905187 1104 42-43 0.15231006747657005 1104 44-45 0.07797371977236622 1104 46-47 0.15576788124246121 1104 48-49 0.10442267307023911 1104 50-51 0.15130390070871869 1104 52-53 0.011291897124834804 1104 54-55 -0.037447499231411996 1104 56-57 0.02923293890048484 1104 58-59 0.05569010307531386 1104 60-61 -0.014753118341232607 1104 62-63 -0.14458455972863504 1104 64-65 0.07068123398237702 1104 66-67 0.003837275667123663 1104 68-69 0.03840785831577165 1104 70-71 0.015047937727267424 1104 72-73 -0.28512027593370703 1104 74-75 -0.393632440982401 1104 76-77 -0.22932405863237548 1104 78-79 -0.03383737805779319 1104 80-81 0.10499017016671885 1104 82-83 0.036963032812860064 1104 84-85 -0.06894422677900636 1104 86-87 -0.1689923192099947 1104 88-89 -0.02233242412511771 1104 90-91 0.1211083879169692 1104 92-93 -0.018467634122679044 1104 94-95 0.14129609926857256 1104 96-97 -0.14960361561118418 1104 98-99 -0.6862942806269885 1104 100-101 -0.9840273878906132 1105 1 0.016115384101141217 1105 2 0.10609912290249213 1105 3 0.08389449539649974 1105 4 0.07516993624887647 1105 5 -0.011424707751238827 1105 6 0.03652304419060215 1105 7 0.12577297268840226 1105 8 0.11214013698327818 1105 9 0.20636467131102165 1105 10-11 0.14089707075243751 1105 12-13 0.12843241691238205 1105 14-15 0.12244967817384378 1105 16-17 0.15062002514910233 1105 18-19 0.16581302564431866 1105 20-21 0.20348618238971028 1105 22-23 0.2150893541541663 1105 24-25 0.1670910975069475 1105 26-27 0.20963573308024763 1105 28-29 0.17543987722986998 1105 30-31 0.27543050353396836 1105 32-33 0.21069476767410578 1105 34-35 0.18661044028638685 1105 36-37 0.2625522064054664 1105 38-39 0.19331828261920947 1105 40-41 0.24550536145508062 1105 42-43 0.2022140372818555 1105 44-45 0.3001340957698986 1105 46-47 0.1786940841119744 1105 48-49 0.17667270201550878 1105 50-51 0.1822875693841084 1105 52-53 0.2940728040188887 1105 54-55 0.14951573624936287 1105 56-57 0.05689641467344586 1105 58-59 0.025009198843598313 1105 60-61 -0.05779643966099002 1105 62-63 -0.08109333896877757 1105 64-65 -0.040436103027879255 1105 66-67 0.10932498956029235 1105 68-69 -0.011025866918991767 1105 70-71 -0.09072040599024689 1105 72-73 -0.22809050657334495 1105 74-75 -0.19373113352293814 1105 76-77 -0.2676089011546239 1105 78-79 -0.440735608959951 1105 80-81 -0.17138764023339448 1105 82-83 -0.13245085484976826 1105 84-85 -0.3388174000537596 1105 86-87 -0.23299697026623534 1105 88-89 -0.33639651359975176 1105 90-91 -0.16045449576266435 1105 92-93 -0.2103631748172532 1105 94-95 -0.3039464125742235 1105 96-97 -0.2964008755000975 1105 98-99 -0.2929980054964574 1105 100-101 -0.9839852856775835 1106 1 -0.026590052432844402 1106 2 0.009998687263653494 1106 3 0.03008325233390252 1106 4 0.014572872335286036 1106 5 0.03252289623515736 1106 6 0.006658509966101178 1106 7 0.03291088520257546 1106 8 0.04541160562325075 1106 9 0.08516338229748044 1106 10-11 0.11498968876281168 1106 12-13 0.09105430627205635 1106 14-15 0.05615201152706817 1106 16-17 0.07883256319291121 1106 18-19 0.04260257627987585 1106 20-21 0.13510804191110282 1106 22-23 0.010242722895590362 1106 24-25 0.04379456471468046 1106 26-27 0.044136986287860225 1106 28-29 0.0970065359396628 1106 30-31 0.017651963222455436 1106 32-33 0.04120171999253586 1106 34-35 0.006280727330612024 1106 36-37 -0.045988552680292116 1106 38-39 0.02179667970698773 1106 40-41 0.02649867768113978 1106 42-43 -0.040423218790238025 1106 44-45 0.14703658428215505 1106 46-47 0.07000801261946066 1106 48-49 -0.08076165377609357 1106 50-51 -0.008731706142533824 1106 52-53 0.03800056555137843 1106 54-55 0.07324253698934768 1106 56-57 0.02698264729264821 1106 58-59 -0.13875415378515044 1106 60-61 -0.09692605713428293 1106 62-63 -0.07210082837618614 1106 64-65 -0.10530854439566895 1106 66-67 -0.1770225070083029 1106 68-69 -0.3490941368957152 1106 70-71 -0.27928995901286413 1106 72-73 -0.19204795719099366 1106 74-75 -0.372508062567519 1106 76-77 -0.40994180680599257 1106 78-79 -0.3456827952104753 1106 80-81 -0.1339264299929006 1106 82-83 -0.1715594363381001 1106 84-85 -0.26690312542226025 1106 86-87 -0.34817167337538635 1106 88-89 -0.29780667054908605 1106 90-91 -0.07038571853524189 1106 92-93 -0.05138125681283867 1106 94-95 -0.04457734293168514 1106 96-97 -0.40167986338575545 1106 98-99 -0.29042743797243986 1106 100-101 -0.9511951084382204 1107 1 0.02827765584109443 1107 2 0.00660899689471961 1107 3 0.04179078497916322 1107 4 0.03321488636277081 1107 5 0.03496744205257585 1107 6 0.030125465545729924 1107 7 0.07625866418415939 1107 8 0.04823249859240519 1107 9 0.11257799140964408 1107 10-11 0.10949159900972205 1107 12-13 0.10846863027273912 1107 14-15 0.12337844706336654 1107 16-17 0.13482232769083424 1107 18-19 0.07706613903291526 1107 20-21 0.07372710446456665 1107 22-23 0.07826832590637167 1107 24-25 0.11451817764733363 1107 26-27 0.07789489813139028 1107 28-29 0.11935115018383868 1107 30-31 0.12085633113031236 1107 32-33 0.08043277433478835 1107 34-35 0.05594344045143629 1107 36-37 0.175438572825783 1107 38-39 0.10534503461375522 1107 40-41 0.19880266353960963 1107 42-43 0.13571993568316287 1107 44-45 0.18574930595632821 1107 46-47 0.13239624891985358 1107 48-49 0.11771456792658341 1107 50-51 0.07828773846030401 1107 52-53 0.14979862109110087 1107 54-55 0.023020720259005145 1107 56-57 -0.006097982233498556 1107 58-59 -0.0274903971563063 1107 60-61 -0.0887864095320765 1107 62-63 -0.00887379410578859 1107 64-65 -0.1455510317082016 1107 66-67 -0.08090715090406064 1107 68-69 -0.045113410431667944 1107 70-71 -0.23351474090258506 1107 72-73 -0.3379146960859032 1107 74-75 -0.3860015335146585 1107 76-77 -0.3909977171669432 1107 78-79 -0.23742881790534653 1107 80-81 -0.16138195260223753 1107 82-83 -0.16769375235566386 1107 84-85 -0.37724226552603923 1107 86-87 -0.28957941130091314 1107 88-89 -0.4255023995791376 1107 90-91 -0.34268614050022705 1107 92-93 -0.3586151052475728 1107 94-95 -0.35477278685025837 1107 96-97 -0.5141386647771 1107 98-99 -0.5954439744381972 1107 100-101 -1.3003890982882496 1108 1 -0.11080385333782061 1108 2 -0.028564661152636006 1108 3 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-0.256392109146244 2316 84-85 -0.2302759672967447 2316 86-87 -0.3212089122395625 2316 88-89 -0.470570546848176 2316 90-91 -0.6542882102611074 2316 92-93 -0.43362423170585274 2316 94-95 -0.23903309477096712 2316 96-97 -0.2097503568823953 2316 98-99 -0.11673548865017835 2316 100-101 0.9353952113543471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 18.0 5 47.0 6 86.0 7 218.0 8 571.0 9 1112.0 10 2013.0 11 3137.0 12 4786.0 13 6637.0 14 8733.0 15 11607.0 16 14554.0 17 19857.0 18 20211.0 19 16303.0 20 16498.0 21 17094.0 22 18681.0 23 20788.0 24 23735.0 25 27272.0 26 32065.0 27 38374.0 28 46031.0 29 56171.0 30 68258.0 31 83590.0 32 104358.0 33 135128.0 34 187124.0 35 277729.0 36 443668.0 37 664927.0 38 422431.0 39 20241.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.370002762216025 19.319131593541698 14.624684789753964 23.686180854488313 2 15.413489615186023 20.171844178217885 40.39390048758716 24.020765719008935 3 19.85696116750845 23.49515522229262 29.527433840275673 27.120449769923255 4 13.234254694549977 16.50991218740461 36.869669847242115 33.3861632708033 5 13.960571572542316 36.983455582519355 34.47519438988707 14.580778455051258 6 32.72541000355003 36.01695201347803 17.046076826337455 14.211561156634486 7 28.85033409012811 32.099481852028084 20.645572359661003 18.404611698182805 8 25.723038697455387 36.005793762111054 19.565709485330988 18.70545805510257 9 25.55815243521729 17.534481306553058 20.51156673576017 36.39579952246949 10-11 25.01951805593411 26.472596009823523 27.271885719605937 21.236000214636427 12-13 25.94090888577042 23.47688980444987 27.80316418158668 22.77903712819303 14-15 22.334923281802894 25.305731188250313 26.019244624928817 26.340100905017977 16-17 22.236675761083653 28.35977999269722 26.885027438723736 22.518516807495388 18-19 22.189231150284233 27.309429326514245 28.627275670285755 21.874063852915768 20-21 22.667436846607092 26.38057597187836 28.89361673396561 22.058370447548935 22-23 22.26852936023775 26.401861935473324 28.7075333477135 22.622075356575426 24-25 22.429317377253774 26.494763078799373 28.844501428186625 22.231418115760228 26-27 22.150962398528197 26.682460547577275 28.70635286441629 22.46022418947824 28-29 22.058054544314167 27.324359231082628 28.504486666153767 22.113099558449438 30-31 22.259414419232563 26.747430137441942 28.409565393216425 22.583590050109077 32-33 21.995440396537315 26.749069538483628 28.543611624554625 22.71187844042443 34-35 22.697927943232568 26.83935820332186 28.29618093588359 22.16653291756198 36-37 22.73182494592231 26.472236386026655 28.465757548883193 22.33018111916784 38-39 22.26156837237737 26.834305435597784 28.453930882995852 22.450195309029 40-41 22.646755122011548 26.729295832361196 28.383209038700706 22.240740006926547 42-43 22.39217559388317 26.757838390709253 28.124330312163824 22.72565570324375 44-45 22.48989854119654 26.855860978340058 28.23818831180369 22.416052168659714 46-47 22.850959779508983 27.107558668986815 27.738639749827776 22.302841801676426 48-49 22.641561398517997 27.172499866291844 27.67844086163845 22.50749787355171 50-51 22.510931349755396 27.350131368092022 27.802932403756238 22.33600487839634 52-53 22.952292078956354 27.139248225251688 27.525273972383683 22.383185723408268 54-55 22.49037956177922 27.109860091874342 27.771999256590384 22.62776108975605 56-57 22.589513080398486 27.289309602005364 27.60207856412341 22.519098753472743 58-59 22.733176617750793 27.002645685968268 27.566359809738046 22.697817886542893 60-61 22.33865833543444 27.185087222251543 27.993121509515227 22.483132932798792 62-63 22.388366534062754 27.170867152839563 28.02594296166036 22.41482335143733 64-65 22.155878968061124 27.320939401052136 27.758273011098677 22.764908619788063 66-67 22.17094899127899 27.29287646201558 27.578891894091313 22.957282652614115 68-69 22.480537923064293 27.003742802780735 27.78075107660663 22.734968197548344 70-71 22.488612972549422 26.986602086133633 27.511911181630083 23.012873759686858 72-73 22.4073856252583 26.96713042112449 27.70693314750462 22.918550806112588 74-75 22.25365761438718 27.460190095381726 27.351326072888966 22.934826217342124 76-77 22.36094966685969 26.99910863803021 27.563234768950156 23.076706926159947 78-79 22.447486857720047 27.052145112237614 27.569626272689113 22.930741757353225 80-81 22.620928064863122 27.071188170497386 27.362547702616407 22.945336062023085 82-83 22.5072590821592 27.097298175128397 27.573122970681872 22.82231977203053 84-85 22.349408914732123 27.25903951620096 27.453633734307818 22.937917834759087 86-87 22.500486917266755 27.207976371839372 27.287750762392378 23.003785948501495 88-89 22.29080226698342 27.2092784673151 27.084546206335457 23.415373059366026 90-91 22.311772321192805 27.067920970709437 27.218488126622713 23.401818581475048 92-93 22.50283487102803 26.94259292886686 27.338124511160206 23.216447688944907 94-95 22.22867592422481 26.786039819321616 27.521738157061982 23.463546099391593 96-97 22.21766376380323 26.46414318384413 27.731955300889943 23.5862377514627 98-99 22.08461736369141 26.734507060271724 27.307629983198627 23.873245592838238 100-101 22.542286420638412 25.528263766129534 28.07636741803239 23.853082395199664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 109.5 2 91.0 3 154.5 4 153.5 5 147.5 6 260.5 7 517.5 8 812.5 9 1028.0 10 1041.5 11 999.0 12 1116.0 13 1266.5 14 1435.0 15 1622.0 16 1803.0 17 2066.0 18 2332.5 19 2646.5 20 3176.5 21 3972.5 22 5108.5 23 6615.0 24 8572.5 25 11170.0 26 14741.0 27 19248.0 28 23385.5 29 27542.0 30 32485.5 31 38993.0 32 47649.0 33 52668.0 34 54696.0 35 58772.0 36 64259.0 37 70067.0 38 75319.5 39 79517.5 40 83985.5 41 89149.0 42 91713.5 43 93583.0 44 97983.0 45 103721.5 46 106859.0 47 111590.5 48 119606.0 49 116863.0 50 107712.0 51 103349.0 52 101086.0 53 100247.0 54 97832.0 55 90131.0 56 81415.0 57 72696.0 58 64993.5 59 56806.5 60 46353.5 61 38661.5 62 32333.0 63 23834.0 64 16184.5 65 11883.5 66 9021.5 67 7123.0 68 5391.5 69 4019.0 70 3030.0 71 2028.0 72 1409.0 73 1116.0 74 844.0 75 631.0 76 481.0 77 320.0 78 181.5 79 101.0 80 56.5 81 33.5 82 25.5 83 13.0 84 6.5 85 4.0 86 3.5 87 2.5 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4251151627487819 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010127712227986528 16-17 0.0022565253560601563 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0660749713670031E-4 26-27 0.00362465490264781 28-29 1.776791618945005E-5 30-31 0.0 32-33 1.776791618945005E-5 34-35 0.011904503846931534 36-37 0.02656303470322783 38-39 0.020290960288351957 40-41 0.008866190178535575 42-43 0.008883958094725026 44-45 0.003944477394057911 46-47 0.005667965264434566 48-49 0.0034292078245638596 50-51 7.640203961463522E-4 52-53 0.0 54-55 0.0 56-57 1.0660749713670031E-4 58-59 0.0016346482894294045 60-61 0.0025408120150913573 62-63 0.0014747370437243543 64-65 0.002718491176985858 66-67 0.001847863283702805 68-69 0.004513050712120313 70-71 1.2437541332615035E-4 72-73 0.0 74-75 0.0 76-77 0.09356584665364397 78-79 0.015351479587684845 80-81 4.2642998854680125E-4 82-83 0.0 84-85 0.0 86-87 0.10690955171192096 88-89 0.41429450178940685 90-91 0.4142411980408385 92-93 0.0 94-95 0.0 96-97 1.776791618945005E-5 98-99 1.7767916189450052E-4 100-101 2.6651874284175077E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2814061.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.210436837974 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04482315952757 42.9334711538508 2 14.343981170406654 16.41250857509276 3 4.761736731591549 8.172631155653372 4 2.1240738986157406 4.8607678246378 5 1.081704442466126 3.0942391841532086 6 0.6328073984383655 2.172191261937645 7 0.41229123586459415 1.651115319579712 8 0.29429930331759097 1.346959336312863 9 0.21105666994368244 1.0867179856541553 >10 1.0097074992886514 10.140668743465323 >50 0.0531951569240456 2.0813004925703495 >100 0.02579415540963867 2.736171585235343 >500 0.002295610871460193 0.9533348780232437 >1k 0.0021094802600632083 1.9792578051190879 >5k 1.2408707412136518E-4 0.3786646987143421 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5363 0.19057866904804124 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5305 0.18851759077006502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4283 0.15219997007882913 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7767916189450052E-5 0.0 12-13 0.0 0.0 0.0 3.5535832378900104E-5 3.7312623997845106E-4 14-15 0.0 0.0 0.0 3.5535832378900104E-5 5.152695694940515E-4 16-17 0.0 0.0 0.0 7.107166475780021E-5 5.685733180624017E-4 18-19 0.0 0.0 0.0 1.7767916189450052E-4 5.685733180624017E-4 20-21 0.0 0.0 0.0 1.7767916189450052E-4 7.107166475780021E-4 22-23 0.0 0.0 0.0 2.8428665903120084E-4 7.640203961463523E-4 24-25 0.0 0.0 0.0 3.5535832378900104E-4 8.173241447147024E-4 26-27 0.0 0.0 0.0 6.041091504413018E-4 8.706278932830525E-4 28-29 0.0 0.0 0.0 0.002132149942734006 9.061637256619526E-4 30-31 0.0 0.0 0.0 0.00696502314626442 9.239316418514027E-4 32-33 0.0 0.0 0.0 0.015404783336253194 9.594674742303028E-4 34-35 0.0 0.0 0.0 0.028659648813582933 9.594674742303028E-4 36-37 0.0 0.0 0.0 0.0529306223283717 9.594674742303028E-4 38-39 0.0 0.0 0.0 0.09395674080981187 9.594674742303028E-4 40-41 0.0 0.0 0.0 0.1403310020642765 9.594674742303028E-4 42-43 0.0 0.0 0.0 0.18677633498349894 9.594674742303028E-4 44-45 0.0 0.0 0.0 0.2448774209230006 9.594674742303028E-4 46-47 0.0 0.0 0.0 0.31271532493432086 9.772353904197529E-4 48-49 0.0 0.0 0.0 0.38830004040424143 0.001030539138988103 50-51 0.0 0.0 0.0 0.47699747802197606 0.001066074971367003 52-53 0.0 0.0 0.0 0.5654994685616268 0.0011904503846931534 54-55 0.0 0.0 0.0 0.6594739772876281 0.0012082183008826034 56-57 0.0 0.0 0.0 0.7748765929381062 0.0012259862170720536 58-59 3.5535832378900104E-5 0.0 0.0 0.9042270227973026 0.0012437541332615035 60-61 3.5535832378900104E-5 1.7767916189450052E-5 0.0 1.048822324747047 0.0012792899656404037 62-63 3.5535832378900104E-5 3.5535832378900104E-5 0.0 1.195052274986221 0.0014214332951560042 64-65 3.5535832378900104E-5 3.5535832378900104E-5 0.0 1.347127869651724 0.0014214332951560042 66-67 3.5535832378900104E-5 3.5535832378900104E-5 0.0 1.526370608170896 0.0014214332951560042 68-69 3.5535832378900104E-5 3.5535832378900104E-5 0.0 1.7363163058654378 0.0014214332951560042 70-71 3.5535832378900104E-5 3.5535832378900104E-5 0.0 1.96692609008831 0.0015102728761032542 72-73 3.5535832378900104E-5 3.5535832378900104E-5 0.0 2.212869585982678 0.0015813445408610544 74-75 3.5535832378900104E-5 3.5535832378900104E-5 0.0 2.457977989816141 0.0016524162056188547 76-77 3.5535832378900104E-5 5.330374856835016E-5 0.0 2.7205877910962126 0.0017057199541872048 78-79 3.5535832378900104E-5 7.107166475780021E-5 0.0 3.0366079484417714 0.0017057199541872048 80-81 3.5535832378900104E-5 7.107166475780021E-5 0.0 3.3870267915301056 0.001741255786566105 82-83 5.330374856835016E-5 7.107166475780021E-5 0.0 3.750700500095769 0.001776791618945005 84-85 7.107166475780021E-5 7.107166475780021E-5 0.0 4.116986092341282 0.001776791618945005 86-87 7.107166475780021E-5 7.107166475780021E-5 0.0 4.4914982297825095 0.0018300953675133552 88-89 7.107166475780021E-5 7.107166475780021E-5 0.0 4.906201393644274 0.0019011670322711554 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 165 8.139614E-8 25.999596 1 GTACATG 7475 0.0 20.724316 1 TACATGG 7220 0.0 20.639078 2 GTATCAA 13540 0.0 20.066158 1 TAAGGTG 1570 0.0 19.647692 5 ACATGGG 7140 0.0 19.474543 3 GAGTACT 7830 0.0 19.001553 12-13 GTAAGCG 245 0.0 18.401754 94-95 GTACTTT 8135 0.0 18.201633 14-15 GGTATCA 10820 0.0 18.19411 1 AGAGTAC 11110 0.0 18.133303 10-11 ATCAACG 15210 0.0 17.534966 3 CAACGCA 15305 0.0 17.48814 5 TCAACGC 15280 0.0 17.485697 4 TATCAAC 15435 0.0 17.279354 2 AGTACTT 8290 0.0 17.11709 12-13 AACGCAG 15700 0.0 17.07838 6 GTAAGGT 1425 0.0 16.651487 4 GTGTAGC 1730 0.0 16.531536 1 CATGGGG 4485 0.0 16.189255 4 >>END_MODULE