FastQCFastQC Report
Fri 27 May 2016
SRR939139_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939139_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048143
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53260.2600404366296689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.21004392759685236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.12894607456608254No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51050.036.4506531
GTATCAA79050.032.787311
ATCAACG92800.027.512573
TCAACGC93950.027.2766384
CAACGCA94500.027.067765
TATCAAC96150.026.701792
AACGCAG97000.026.460696
GTACATG74650.023.9732281
ACGCAGA110350.023.0020247
TACATGG77100.022.9777572
CGCAGAG110900.022.6738878
ACATGGG77650.021.5949483
GAGTACT57350.021.0149312-13
TACCTGG17150.020.7151222
GCAGAGT119700.020.531739
GTACCTG19500.019.7181151
GTGTAGC11650.019.5582641
GTACTTT62100.019.37008914-15
CATGGGG43150.018.5522374
AGAGTAC99850.018.32963410-11
Produced by FastQC (version 0.11.5)