##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939139_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048143 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.958604452911736 33.0 31.0 34.0 30.0 34.0 2 32.073387453903365 34.0 31.0 34.0 30.0 34.0 3 32.13040056285132 34.0 31.0 34.0 30.0 34.0 4 35.56564800407003 37.0 35.0 37.0 33.0 37.0 5 35.606783803669956 37.0 35.0 37.0 33.0 37.0 6 35.682208712965846 37.0 36.0 37.0 35.0 37.0 7 35.59884783435532 37.0 35.0 37.0 33.0 37.0 8 35.580477535015866 37.0 35.0 37.0 33.0 37.0 9 37.24692562970456 39.0 37.0 39.0 34.0 39.0 10-11 37.15172695461206 39.0 37.0 39.0 34.0 39.0 12-13 37.056306371186 39.0 37.0 39.0 33.0 39.0 14-15 38.358188368683244 40.0 38.0 41.0 33.5 41.0 16-17 38.290384021037596 40.0 38.0 41.0 33.0 41.0 18-19 38.20429847915892 40.0 38.0 41.0 33.0 41.0 20-21 38.09250135366525 40.0 38.0 41.0 33.0 41.0 22-23 38.004673013554225 40.0 38.0 41.0 33.0 41.0 24-25 38.047959297763875 40.0 38.0 41.0 33.0 41.0 26-27 37.97380407520373 40.0 38.0 41.0 33.0 41.0 28-29 37.84909549772648 40.0 38.0 41.0 33.0 41.0 30-31 37.68581539472586 40.0 38.0 41.0 32.5 41.0 32-33 37.49960818165529 40.0 37.5 41.0 32.0 41.0 34-35 37.19477302121971 40.0 37.0 41.0 31.0 41.0 36-37 36.94601695291784 40.0 37.0 41.0 30.0 41.0 38-39 36.71626639350866 40.0 36.5 41.0 30.0 41.0 40-41 36.572063571733025 40.0 36.0 41.0 30.0 41.0 42-43 36.38734014177721 40.0 36.0 41.0 30.0 41.0 44-45 36.139408234678925 39.0 36.0 41.0 29.5 41.0 46-47 35.804556615431636 39.0 35.0 41.0 27.5 41.0 48-49 35.815424264809636 39.0 35.5 41.0 27.0 41.0 50-51 34.84124008919299 38.0 34.0 40.0 25.5 40.5 52-53 35.00791741592262 38.5 35.0 40.0 26.5 40.5 54-55 35.51183218163966 39.0 35.0 41.0 27.0 41.0 56-57 35.4011673013066 39.0 35.0 41.0 26.0 41.0 58-59 35.21509679744041 39.0 35.0 41.0 26.0 41.0 60-61 34.929663114343086 38.5 35.0 41.0 26.0 41.0 62-63 34.564235505040415 38.0 34.0 40.0 25.5 41.0 64-65 34.18999088442555 37.5 34.0 40.0 23.5 41.0 66-67 33.803151684232986 37.0 34.0 40.0 23.0 41.0 68-69 33.40315251425316 36.0 33.5 39.0 22.0 41.0 70-71 33.003305189139624 36.0 33.0 39.0 21.5 41.0 72-73 32.55080284921512 35.0 33.0 38.5 20.0 40.0 74-75 32.086629205089686 35.0 32.0 37.5 18.5 39.5 76-77 31.64856042766545 35.0 32.0 37.0 17.5 39.0 78-79 31.223500019285765 35.0 31.5 36.5 14.0 39.0 80-81 30.79814275663369 35.0 31.0 36.0 8.5 37.5 82-83 30.38560198189287 35.0 31.0 35.5 7.0 37.0 84-85 30.0128982693103 34.0 31.0 35.0 2.0 37.0 86-87 29.630151312676897 34.0 30.0 35.0 2.0 36.0 88-89 29.239212301094213 34.0 30.0 35.0 2.0 36.0 90-91 28.876627022624888 34.0 29.0 35.0 2.0 36.0 92-93 28.476323186418135 34.0 29.0 35.0 2.0 35.0 94-95 28.03189279264192 34.0 29.0 35.0 2.0 35.0 96-97 27.481800098918875 34.0 27.0 35.0 2.0 35.0 98-99 26.791527984130013 34.0 26.0 35.0 2.0 35.0 100-101 24.29991948804356 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2759504562354742 1101 2 0.37970856538750297 1101 3 0.3483313817775979 1101 4 0.17846619045705125 1101 5 0.32729182082467645 1101 6 0.3998174163640371 1101 7 0.3225067109619957 1101 8 0.40070844201463984 1101 9 0.45066937514996397 1101 10-11 0.4426493695367171 1101 12-13 0.33364439977201243 1101 14-15 0.5164254779475925 1101 16-17 0.5232374763144705 1101 18-19 0.6187098857029554 1101 20-21 0.5499824758227376 1101 22-23 0.5539143661139079 1101 24-25 0.6019820914695373 1101 26-27 0.5564888429230237 1101 28-29 0.4624240141456539 1101 30-31 0.4435376693556847 1101 32-33 0.49257942277759525 1101 34-35 0.3262479875390767 1101 36-37 0.3363736415476737 1101 38-39 0.37591613413494684 1101 40-41 0.47100330638674137 1101 42-43 0.47183228920835063 1101 44-45 0.3830104678797568 1101 46-47 0.3154094962552847 1101 48-49 0.5111255245831572 1101 50-51 0.46234487070025665 1101 52-53 0.6774721160181443 1101 54-55 0.6908977549610924 1101 56-57 0.6420584392963846 1101 58-59 0.6116691442356768 1101 60-61 0.5888436953050658 1101 62-63 0.634050763722712 1101 64-65 0.5826004738923487 1101 66-67 0.37385101747113936 1101 68-69 0.4951124405700469 1101 70-71 0.4334508566095039 1101 72-73 0.280996021990795 1101 74-75 0.1677478235000862 1101 76-77 0.21116392038260656 1101 78-79 0.29506780754488915 1101 80-81 0.12275954544829659 1101 82-83 0.025520878684918813 1101 84-85 0.288590733162593 1101 86-87 0.14153217978745758 1101 88-89 0.18735690047658338 1101 90-91 0.09843801148246456 1101 92-93 0.00900655263315997 1101 94-95 -0.08621447935085413 1101 96-97 -0.2064638983179954 1101 98-99 -0.2206939691854508 1101 100-101 -0.2236456886739404 1102 1 0.13658196649479848 1102 2 0.2621523143301516 1102 3 0.33398494535155976 1102 4 0.3147457056466507 1102 5 0.33424858424397286 1102 6 0.3794673426515871 1102 7 0.42631407979008884 1102 8 0.34025849397587393 1102 9 0.42542739288929 1102 10-11 0.34006611072143045 1102 12-13 0.370994860172722 1102 14-15 0.4812656971514997 1102 16-17 0.4777244195438044 1102 18-19 0.6558605068209715 1102 20-21 0.5999030234699845 1102 22-23 0.6007341573022487 1102 24-25 0.5352274632944614 1102 26-27 0.609300791590158 1102 28-29 0.5863646997922629 1102 30-31 0.5560332889027748 1102 32-33 0.5181701797198599 1102 34-35 0.5466803835561862 1102 36-37 0.5136014794769608 1102 38-39 0.5844741796674882 1102 40-41 0.44795593103327747 1102 42-43 0.5646004635564168 1102 44-45 0.45588232301667375 1102 46-47 0.4839824655377356 1102 48-49 0.4788952094322312 1102 50-51 0.4139472101642028 1102 52-53 0.42191770173000975 1102 54-55 0.4974407921556647 1102 56-57 0.5249018668044272 1102 58-59 0.43904259446165383 1102 60-61 0.31390804264778893 1102 62-63 0.2291938987977602 1102 64-65 0.29477269517470717 1102 66-67 0.43882567126430416 1102 68-69 0.3006733500124952 1102 70-71 0.13885542919813787 1102 72-73 0.04408345583085094 1102 74-75 0.06620922717845446 1102 76-77 0.08056177426277955 1102 78-79 0.11508602418698644 1102 80-81 0.10876067530304567 1102 82-83 0.17251619008980867 1102 84-85 0.03417221562869699 1102 86-87 -0.06526235582411744 1102 88-89 -0.014061459965205358 1102 90-91 -0.1564223275948038 1102 92-93 -0.11960037271183666 1102 94-95 -0.3568953527829528 1102 96-97 -0.4554012608623985 1102 98-99 -0.670957853710668 1102 100-101 -0.8177276967943925 1103 1 0.17525197465425535 1103 2 0.2772560560249886 1103 3 0.24976038537953826 1103 4 0.24264959887429427 1103 5 0.2961380818544157 1103 6 0.2243997357043881 1103 7 0.2529241159248343 1103 8 0.31954658116552537 1103 9 0.3276668730151826 1103 10-11 0.38985775310398907 1103 12-13 0.32811690236878377 1103 14-15 0.4760108597578636 1103 16-17 0.41496433678357647 1103 18-19 0.42537399906948536 1103 20-21 0.3586642427263058 1103 22-23 0.23584460140995844 1103 24-25 0.20380407478764084 1103 26-27 0.35780294801864443 1103 28-29 0.3478076127191798 1103 30-31 0.4321268021327569 1103 32-33 0.3002069847087867 1103 34-35 0.1834219909696415 1103 36-37 0.016227805389071648 1103 38-39 0.23679743995423053 1103 40-41 0.2112259799900329 1103 42-43 0.25936602481064597 1103 44-45 0.10719876432684572 1103 46-47 0.0679629702585629 1103 48-49 0.22392158196981882 1103 50-51 0.21814933162358585 1103 52-53 0.27685708860493463 1103 54-55 0.25184180043169135 1103 56-57 0.23425756094126626 1103 58-59 0.10102024337177795 1103 60-61 0.30341002423072894 1103 62-63 0.24048746448140434 1103 64-65 0.19638710244827706 1103 66-67 0.10081789063718816 1103 68-69 -0.03824508453698883 1103 70-71 0.005416217168765058 1103 72-73 -0.037570145987551484 1103 74-75 -0.11169175195664138 1103 76-77 -0.17921500624776954 1103 78-79 -0.19811845768736092 1103 80-81 -0.22496193222697158 1103 82-83 -0.2944933681337645 1103 84-85 -0.11958404967028713 1103 86-87 -0.13617825511307657 1103 88-89 -0.012783630381338895 1103 90-91 -0.12188814528917291 1103 92-93 -0.47904713224092177 1103 94-95 -0.4479680302511859 1103 96-97 -0.3782543031750265 1103 98-99 -0.48305643746169125 1103 100-101 -0.6209688962348849 1104 1 0.3536430562031079 1104 2 0.2622669721910924 1104 3 0.25062180097438613 1104 4 0.17376010667398134 1104 5 0.34483379796622415 1104 6 0.30460951839915396 1104 7 0.28650716362791684 1104 8 0.3582299671000726 1104 9 0.32268248369831554 1104 10-11 0.34643191516098426 1104 12-13 0.3152527944966934 1104 14-15 0.39169153644704124 1104 16-17 0.4144825831835064 1104 18-19 0.4553961424841546 1104 20-21 0.436802216278636 1104 22-23 0.42384459575391276 1104 24-25 0.4490173642300732 1104 26-27 0.44510385524819895 1104 28-29 0.4203962994987336 1104 30-31 0.40499334210402793 1104 32-33 0.3919238487714054 1104 34-35 0.41633847381485367 1104 36-37 0.5461815106638923 1104 38-39 0.6689391860883234 1104 40-41 0.5986083342620532 1104 42-43 0.5232147086490642 1104 44-45 0.5790993932790016 1104 46-47 0.6175462611715616 1104 48-49 0.5972507863905818 1104 50-51 0.6121723064768076 1104 52-53 0.6062714900257333 1104 54-55 0.5757876551165921 1104 56-57 0.5027522612274637 1104 58-59 0.5819739373345172 1104 60-61 0.7132453202115485 1104 62-63 0.618487147742897 1104 64-65 0.614078785800281 1104 66-67 0.6234030423888726 1104 68-69 0.6262294406380633 1104 70-71 0.5861678489376416 1104 72-73 0.5398766589128527 1104 74-75 0.480059896780368 1104 76-77 0.5472582519732931 1104 78-79 0.5127320983018286 1104 80-81 0.5132862210744626 1104 82-83 0.5314090537845431 1104 84-85 0.7408957525943904 1104 86-87 0.5904980551096592 1104 88-89 0.6400409982993303 1104 90-91 0.5618529888608919 1104 92-93 0.4853654592455996 1104 94-95 0.24338670555496478 1104 96-97 0.1619453269196569 1104 98-99 0.024525773146788055 1104 100-101 -0.08855071092023792 1105 1 0.05924729444317123 1105 2 0.09103986199378511 1105 3 0.237963025719786 1105 4 0.15482969517756828 1105 5 0.21033328449892963 1105 6 0.14871499049346681 1105 7 0.13426657409301157 1105 8 0.1009100474155531 1105 9 0.1434941671046701 1105 10-11 0.127184801635444 1105 12-13 0.2872343403288582 1105 14-15 0.34329738077776994 1105 16-17 0.3242394744838961 1105 18-19 0.30048763053208205 1105 20-21 0.3499975046414292 1105 22-23 0.17611236402141373 1105 24-25 0.25198715396028604 1105 26-27 0.2932181219753076 1105 28-29 0.4265602335121699 1105 30-31 0.33695810445296814 1105 32-33 0.35692226676663097 1105 34-35 0.28836195098472217 1105 36-37 0.43116524721686034 1105 38-39 0.45484699954970864 1105 40-41 0.35754351366905723 1105 42-43 0.10291257858089864 1105 44-45 0.2431339926540872 1105 46-47 0.329519710861625 1105 48-49 0.42224246581182 1105 50-51 0.2465017554136395 1105 52-53 0.33660769067430607 1105 54-55 0.3708883395936695 1105 56-57 0.22368771324291004 1105 58-59 0.2231149021713108 1105 60-61 0.2157584634415528 1105 62-63 0.2684453258864963 1105 64-65 0.28117530341911134 1105 66-67 0.25423921676192407 1105 68-69 0.31265652390297305 1105 70-71 0.06546301927959775 1105 72-73 0.17269362369709285 1105 74-75 0.23518020030631703 1105 76-77 0.2446493399765508 1105 78-79 0.24015103287361228 1105 80-81 -0.007817667920257065 1105 82-83 0.0928039769177218 1105 84-85 0.17099166916088393 1105 86-87 -0.052858811229000935 1105 88-89 -0.14848109174246815 1105 90-91 -0.13603117554498567 1105 92-93 -0.009377933010568995 1105 94-95 0.0661041846823558 1105 96-97 -0.006984455059285466 1105 98-99 -0.1251157303380026 1105 100-101 -0.09583669140191375 1106 1 0.06545986155638772 1106 2 0.055368890279375194 1106 3 0.09259658289315809 1106 4 0.017491193647707348 1106 5 0.19431711814731756 1106 6 0.12365903925133637 1106 7 0.075933672553262 1106 8 0.02616855149146602 1106 9 0.08069331985991823 1106 10-11 0.06338223712351265 1106 12-13 0.13936459066479756 1106 14-15 0.18300039271552748 1106 16-17 0.21180805404434722 1106 18-19 0.19491725663916526 1106 20-21 0.1460546023941518 1106 22-23 0.16904576619623413 1106 24-25 0.2886402478423804 1106 26-27 0.22957889379602392 1106 28-29 0.04639728645129537 1106 30-31 0.22243198102908934 1106 32-33 0.23941334836614914 1106 34-35 0.21463926872333872 1106 36-37 0.11824308131922123 1106 38-39 0.20572148018093372 1106 40-41 0.15500751326294449 1106 42-43 0.003153401038517245 1106 44-45 0.07142792230268213 1106 46-47 0.16033207108215208 1106 48-49 0.14850629054904374 1106 50-51 0.11958968796231062 1106 52-53 0.08890077760211312 1106 54-55 -0.002662642252317937 1106 56-57 0.07096640106036034 1106 58-59 0.07379669327387717 1106 60-61 0.23450650228772218 1106 62-63 0.24040145074909702 1106 64-65 -0.059815330123335286 1106 66-67 0.029659123962687772 1106 68-69 0.11921317570278944 1106 70-71 0.10318529543582855 1106 72-73 0.06935932682844026 1106 74-75 -0.06233508847415692 1106 76-77 -1.0384199143587125E-4 1106 78-79 -0.017814243558007092 1106 80-81 0.14621178154161285 1106 82-83 0.12175317195366375 1106 84-85 0.21891681114644967 1106 86-87 0.1683041066364943 1106 88-89 0.27169544300753046 1106 90-91 0.23414090099110751 1106 92-93 0.15468302379315446 1106 94-95 -0.008595753849522936 1106 96-97 -0.003279271616275281 1106 98-99 0.16568698751544986 1106 100-101 -0.22704271585607216 1107 1 0.0840526393830281 1107 2 0.10648422309994032 1107 3 0.14170538929207765 1107 4 0.15007724373919018 1107 5 0.10440470477949049 1107 6 0.17808384433053703 1107 7 0.06901555377726964 1107 8 0.06917186452229629 1107 9 0.16579250997968842 1107 10-11 0.26010939274140554 1107 12-13 0.2696211738965033 1107 14-15 0.2291144063224948 1107 16-17 0.2264742014983625 1107 18-19 0.2881467677777536 1107 20-21 0.10571666003183822 1107 22-23 0.24833838302289024 1107 24-25 0.27488420525948953 1107 26-27 0.28971813056359963 1107 28-29 0.3401965758552308 1107 30-31 0.44387316723341286 1107 32-33 0.3525491317934737 1107 34-35 0.43084401575713827 1107 36-37 0.4180008187992783 1107 38-39 0.4423731483125479 1107 40-41 0.39166286705875564 1107 42-43 0.45249648858803937 1107 44-45 0.5404880423485778 1107 46-47 0.519539953218171 1107 48-49 0.44277734496927224 1107 50-51 0.441678229308593 1107 52-53 0.5452104059295593 1107 54-55 0.7138402623052471 1107 56-57 0.6135487858223456 1107 58-59 0.6171990747721452 1107 60-61 0.589682649075776 1107 62-63 0.6218844705128959 1107 64-65 0.42841710667006083 1107 66-67 0.40046629967126535 1107 68-69 0.6341485037345294 1107 70-71 0.5809501917466591 1107 72-73 0.41153117943644446 1107 74-75 0.4696407173009973 1107 76-77 0.4787465459692051 1107 78-79 0.5251670825795536 1107 80-81 0.581840512641147 1107 82-83 0.5851377504229802 1107 84-85 0.3912968504025862 1107 86-87 0.3028468631371162 1107 88-89 0.42938900011067105 1107 90-91 0.48573539557470724 1107 92-93 0.6212681567897107 1107 94-95 0.3585613433925303 1107 96-97 0.2770937786038239 1107 98-99 0.2942510883328531 1107 100-101 0.24812175373109824 1108 1 0.03399206426281509 1108 2 0.14007162932054484 1108 3 0.12086676852448619 1108 4 -0.016358196627564325 1108 5 0.11137023468635476 1108 6 0.1700057002333608 1108 7 0.2825446792790771 1108 8 0.26838923494342026 1108 9 0.3026723091635475 1108 10-11 0.26619820312593134 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11 6791.0 12 8203.0 13 11903.0 14 10446.0 15 10277.0 16 12167.0 17 15889.0 18 15217.0 19 10731.0 20 10917.0 21 11698.0 22 12495.0 23 13736.0 24 15563.0 25 17730.0 26 20163.0 27 23217.0 28 27627.0 29 32446.0 30 38789.0 31 47771.0 32 59682.0 33 77904.0 34 108011.0 35 160588.0 36 262758.0 37 437939.0 38 447970.0 39 82950.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08120652834779 18.870781807519272 14.57188644127833 23.47612522285461 2 15.219441230343268 19.828340111053915 40.98768473749226 23.964533921110558 3 19.39195930036753 24.114278061038696 29.38312664329214 27.110635995301635 4 13.15901649441599 16.48331933623443 36.59294643983219 33.76471772951739 5 14.299404336127896 36.68175668771186 33.99092838587949 15.027910590280749 6 32.34970573272625 35.24389999555288 17.675975152826926 14.730419118893948 7 28.987943846122267 32.03868643247713 20.696234577917316 18.277135143483285 8 25.966372055990856 35.504846828678915 19.760143931236765 18.768637184093468 9 26.19228904640334 17.530905586463312 20.567169314134468 35.709636052998874 10-11 25.20681078526645 26.499318633141673 27.02703891426995 21.266831667321927 12-13 25.991523671798372 23.703540616635284 27.711860555017694 22.593075156548654 14-15 22.855657826990356 25.125987563378654 25.9042355231291 26.11411908650189 16-17 22.593031401281934 28.44257294212593 26.474913694547723 22.489481962044415 18-19 22.49421202676568 27.061640143241643 28.65922828001034 21.78491954998233 20-21 23.096771859952792 26.2670631760933 28.71188692442712 21.92427803952679 22-23 22.88263208000264 26.148901405765557 28.54345676809573 22.42500974613607 24-25 22.793873375898755 26.256202636109656 28.5456320168104 22.404291971181195 26-27 22.416870617661065 26.608833563277283 28.48822201340943 22.48607380565222 28-29 22.303809535449336 26.75462289632246 28.608279048166118 22.333288520062087 30-31 22.465714921188923 26.478663939013984 28.46733293738375 22.588288202413338 32-33 22.286305443568097 26.459554496291055 28.516827120473305 22.737312939667547 34-35 22.89191036170312 26.616225325341752 28.211153104738855 22.28071120821627 36-37 22.80060911927212 26.27735829476044 28.29806511158232 22.623967474385122 38-39 22.51292142561593 26.604291289268 28.447003207883558 22.435784077232512 40-41 22.780587710526305 26.45276084270845 28.31899703628794 22.44765441047731 42-43 22.529302796545476 26.486897386738047 28.12958086158954 22.854218955126935 44-45 22.63644996431919 26.683161528213105 28.025834426817053 22.654554080650648 46-47 22.714204369401383 26.856085408693907 27.670487539740623 22.759222682164086 48-49 22.82588933334871 26.87549271777543 27.557501222516684 22.741116726359177 50-51 22.680841574211573 26.989764538508776 27.583858485134034 22.745535402145617 52-53 22.973910240195764 26.949653910348147 27.407164974593755 22.66927087486233 54-55 22.642391148364972 26.88827644065364 27.550235678094264 22.91909673288712 56-57 22.66556532581361 27.148289103935934 27.47535594515597 22.71078962509448 58-59 22.723291481659118 26.822931225743048 27.512841411873634 22.9409358807242 60-61 22.809641496702184 26.75444498450055 27.808779939717976 22.62713357907929 62-63 22.682234007039987 26.868624136834047 27.739240426225482 22.70990142990049 64-65 22.55140341467556 26.754532102690533 27.6406125864943 23.05345189613961 66-67 22.42732707222069 26.988030860934884 27.51005198617824 23.074590080666177 68-69 22.53608161402183 26.774557707268347 27.753773814110666 22.93558686459916 70-71 22.450236593505046 26.78518713376962 27.641625438973406 23.12295083375193 72-73 22.595523201804344 26.680075239810947 27.624425162838833 23.099976395545873 74-75 22.41947970843256 26.993299740013715 27.4058914683029 23.181329083250823 76-77 22.601924598428674 26.76200114077536 27.46513581973296 23.170938441063008 78-79 22.520053449703514 26.84996507324807 27.491695085531298 23.138286391517116 80-81 22.37842647948319 27.011075305899094 27.4909872411024 23.119510973515318 82-83 22.518540723848698 26.890035207259682 27.43183254375395 23.159591525137667 84-85 22.483411964188292 26.765367360476024 27.399255034795132 23.351965640540552 86-87 22.487050902941018 26.74147385918663 27.5858575302489 23.185617707623447 88-89 22.405322960505444 26.623340776494885 27.528370664047614 23.442965598952053 90-91 22.33673838977835 26.744490218192563 27.590846493694904 23.32792489833418 92-93 22.460673914686417 26.494131145513634 27.628642208444205 23.41655273135575 94-95 22.33816381496808 26.390423907154243 27.628512928834482 23.642899349043194 96-97 22.398467628504935 26.288386583210073 27.663221808123765 23.649923980161226 98-99 22.22089996098648 26.261743928980668 27.57497834626832 23.942377763764537 100-101 22.382140155822462 25.768164219811883 28.00263196050882 23.847063663856837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1891.0 1 1064.0 2 264.0 3 295.0 4 253.5 5 249.0 6 418.5 7 804.5 8 1318.0 9 1672.5 10 1745.0 11 1729.0 12 1902.5 13 2134.0 14 2339.0 15 2583.0 16 2740.5 17 2824.0 18 2916.5 19 3001.5 20 3200.0 21 3554.0 22 4067.5 23 4910.5 24 6013.5 25 7423.0 26 9660.5 27 12411.0 28 14925.5 29 17761.5 30 21074.0 31 24670.5 32 29050.5 33 32936.0 34 36413.5 35 40008.5 36 44335.0 37 49571.0 38 53169.0 39 55366.5 40 57541.5 41 61375.0 42 65568.5 43 69230.0 44 72958.5 45 75654.0 46 76959.0 47 79051.0 48 84381.5 49 85837.5 50 82992.0 51 80444.0 52 77020.5 53 74870.5 54 72536.5 55 66758.0 56 60178.0 57 53804.0 58 47444.0 59 41742.0 60 35278.5 61 28785.5 62 23237.0 63 17754.0 64 13276.5 65 10254.5 66 7746.5 67 6045.5 68 4616.0 69 3335.5 70 2451.5 71 1811.5 72 1469.5 73 1187.5 74 842.5 75 633.5 76 469.5 77 270.0 78 155.5 79 114.5 80 75.5 81 46.5 82 42.0 83 32.0 84 27.5 85 24.0 86 13.5 87 13.0 88 14.5 89 9.5 90 8.5 91 7.0 92 5.0 93 5.5 94 3.5 95 2.5 96 3.0 97 3.5 98 3.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34245655698845245 2 0.12059704815532901 3 0.11356628907258917 4 0.12113412002970497 5 0.13973633676945407 6 0.09149751750732249 7 0.1001394922131902 8 0.10575433453621158 9 0.09330403199385981 10-11 0.0922054758871817 12-13 0.09637998909255849 14-15 0.0980644417894649 16-17 0.08678593242756975 18-19 0.10204365613143222 20-21 0.11180859930190422 22-23 0.12013321335473158 24-25 0.13390178322509708 26-27 0.1326567529708619 28-29 0.12782310610147826 30-31 0.11854641008952989 32-33 0.11127152742752826 34-35 0.3333751598399135 36-37 0.4940328873521038 38-39 0.467325767780863 40-41 0.44655085118568383 42-43 0.4770662985934088 44-45 0.48495149020356487 46-47 0.49232402229727124 48-49 0.504774324839623 50-51 0.4922263728655665 52-53 0.4960347007020506 54-55 0.48216848139998036 56-57 0.45973352446582094 58-59 0.4412289571577766 60-61 0.40190064853870067 62-63 0.4708899720380852 64-65 0.5025039755524883 66-67 0.511975970427846 68-69 0.5247680459811643 70-71 0.5163946072124846 72-73 0.5073864471377243 74-75 0.5143927938625379 76-77 0.49095693025340514 78-79 0.46827784973998393 80-81 0.4708655596801591 82-83 0.4846829542663769 84-85 0.4846829542663769 86-87 0.48678241704802844 88-89 0.5006730487080248 90-91 0.5168096172972297 92-93 0.5095835593510805 94-95 0.5139777837777928 96-97 0.5134895366192693 98-99 0.5071911482743149 100-101 0.4940328873521038 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2048143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07981856127837 #Duplication Level Percentage of deduplicated Percentage of total 1 79.52049728480051 47.77577048773444 2 11.978332568127778 14.39312094719537 3 3.6453842632283675 6.570420753626993 4 1.6115847237582521 3.872948711980948 5 0.8456652409126705 2.5403707118806502 6 0.525405811162407 1.8939771483407222 7 0.36633047336294167 1.5406347861178928 8 0.24473646433453541 1.1762977898038132 9 0.17917123909708899 0.9688117982717279 >10 0.9724889904698891 10.676047845155237 >50 0.0687908422253117 2.868787448582543 >100 0.0390077013700234 4.202982310068461 >500 0.0021974600956098326 0.809252116129682 >1k 3.2554964379404207E-4 0.4501495456851791 >5k 8.138741094851052E-5 0.26042759942638105 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5326 0.2600404366296689 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4302 0.21004392759685236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2641 0.12894607456608254 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 4.882471585235992E-5 16-17 0.0 0.0 0.0 0.0 4.882471585235992E-5 18-19 0.0 0.0 0.0 4.882471585235992E-5 4.882471585235992E-5 20-21 0.0 0.0 0.0 9.764943170471984E-5 4.882471585235992E-5 22-23 0.0 0.0 0.0 9.764943170471984E-5 7.323707377853988E-5 24-25 0.0 0.0 0.0 1.220617896308998E-4 9.764943170471984E-5 26-27 0.0 0.0 0.0 5.370718743759591E-4 9.764943170471984E-5 28-29 0.0 0.0 0.0 0.002685359371879795 9.764943170471984E-5 30-31 0.0 0.0 0.0 0.007543418599189607 9.764943170471984E-5 32-33 0.0 0.0 0.0 0.016795702253211812 9.764943170471984E-5 34-35 0.0 0.0 0.0 0.032688147263154964 9.764943170471984E-5 36-37 0.0 0.0 0.0 0.05885819496001988 9.764943170471984E-5 38-39 0.0 0.0 0.0 0.09764943170471983 9.764943170471984E-5 40-41 0.0 0.0 0.0 0.14210433548829354 9.764943170471984E-5 42-43 0.0 0.0 0.0 0.18780426952610244 9.764943170471984E-5 44-45 0.0 0.0 0.0 0.24585685667455837 1.7088650548325972E-4 46-47 4.882471585235992E-5 0.0 0.0 0.3150170666794262 2.4412357926179959E-4 48-49 4.882471585235992E-5 0.0 0.0 0.3939178074968398 2.4412357926179959E-4 50-51 4.882471585235992E-5 0.0 0.0 0.476016567202583 2.929482951141595E-4 52-53 4.882471585235992E-5 0.0 0.0 0.559165058299152 2.929482951141595E-4 54-55 4.882471585235992E-5 0.0 0.0 0.6566680158563147 2.929482951141595E-4 56-57 4.882471585235992E-5 0.0 0.0 0.77025871728683 2.929482951141595E-4 58-59 4.882471585235992E-5 0.0 0.0 0.9028178208259873 2.929482951141595E-4 60-61 7.323707377853988E-5 0.0 0.0 1.0513426064488662 2.929482951141595E-4 62-63 9.764943170471984E-5 0.0 0.0 1.199989453861376 2.929482951141595E-4 64-65 9.764943170471984E-5 0.0 0.0 1.361330727395499 2.929482951141595E-4 66-67 9.764943170471984E-5 0.0 0.0 1.552162129304448 2.929482951141595E-4 68-69 9.764943170471984E-5 0.0 0.0 1.77057949566998 2.929482951141595E-4 70-71 9.764943170471984E-5 0.0 0.0 2.010797097663591 2.929482951141595E-4 72-73 9.764943170471984E-5 0.0 0.0 2.257874572234458 2.929482951141595E-4 74-75 9.764943170471984E-5 0.0 0.0 2.5143263922489787 2.929482951141595E-4 76-77 9.764943170471984E-5 0.0 0.0 2.801098360807815 2.929482951141595E-4 78-79 9.764943170471984E-5 0.0 0.0 3.1297863479259016 2.929482951141595E-4 80-81 9.764943170471984E-5 0.0 0.0 3.4947022742064395 2.929482951141595E-4 82-83 9.764943170471984E-5 0.0 0.0 3.8722882142506654 4.3942244267123926E-4 84-85 9.764943170471984E-5 0.0 0.0 4.258198768347718 4.3942244267123926E-4 86-87 9.764943170471984E-5 0.0 0.0 4.658952036063888 4.8824715852359917E-4 88-89 9.764943170471984E-5 0.0 0.0 5.106113196197726 5.370718743759591E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5105 0.0 36.450653 1 GTATCAA 7905 0.0 32.78731 1 ATCAACG 9280 0.0 27.51257 3 TCAACGC 9395 0.0 27.276638 4 CAACGCA 9450 0.0 27.06776 5 TATCAAC 9615 0.0 26.70179 2 AACGCAG 9700 0.0 26.46069 6 GTACATG 7465 0.0 23.973228 1 ACGCAGA 11035 0.0 23.002024 7 TACATGG 7710 0.0 22.977757 2 CGCAGAG 11090 0.0 22.673887 8 ACATGGG 7765 0.0 21.594948 3 GAGTACT 5735 0.0 21.01493 12-13 TACCTGG 1715 0.0 20.715122 2 GCAGAGT 11970 0.0 20.53173 9 GTACCTG 1950 0.0 19.718115 1 GTGTAGC 1165 0.0 19.558264 1 GTACTTT 6210 0.0 19.370089 14-15 CATGGGG 4315 0.0 18.552237 4 AGAGTAC 9985 0.0 18.329634 10-11 >>END_MODULE