FastQCFastQC Report
Fri 27 May 2016
SRR939139_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939139_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048143
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50980.24890840141533085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43750.21360813185407465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.1353421123427417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47250.038.697791
GTATCAA72950.032.701611
ATCAACG87050.027.2512233
TCAACGC88200.026.8959054
CAACGCA89400.026.4818155
TATCAAC90350.026.3609032
AACGCAG91950.025.7990076
GTACATG71450.023.7249051
TACATGG72200.022.9336382
ACGCAGA103750.022.681847
GTATAGG4200.022.6744841
CGCAGAG105250.022.2684298
ACATGGG72500.021.529933
GAGTACT55600.020.90726512-13
GCAGAGT115900.019.976589
CATGGGG42450.019.558934
GTACTTT61200.019.14923914-15
AGAGTAC96950.018.74376710-11
AGTACTT59900.018.25786812-13
GTACCTG19000.018.0441171
Produced by FastQC (version 0.11.5)