##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939139_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2048143 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53390705629441 34.0 31.0 34.0 31.0 34.0 2 32.74496019076793 34.0 31.0 34.0 31.0 34.0 3 32.8366666780591 34.0 31.0 34.0 31.0 34.0 4 36.28594048364787 37.0 37.0 37.0 35.0 37.0 5 36.245675228731585 37.0 37.0 37.0 35.0 37.0 6 36.27714617582854 37.0 37.0 37.0 35.0 37.0 7 36.23166058229332 37.0 37.0 37.0 35.0 37.0 8 36.21356223662117 37.0 37.0 37.0 35.0 37.0 9 37.96915205627732 39.0 38.0 39.0 35.0 39.0 10-11 37.9109283384998 39.0 38.0 39.0 35.0 39.0 12-13 37.84552494625619 39.0 38.0 39.0 35.0 39.0 14-15 39.23798509186126 41.0 39.0 41.0 36.0 41.0 16-17 39.18075398055702 40.0 39.0 41.0 36.0 41.0 18-19 39.195427760659285 40.5 38.5 41.0 36.0 41.0 20-21 39.257152942934155 41.0 39.0 41.0 36.0 41.0 22-23 39.222430513885016 41.0 39.0 41.0 36.0 41.0 24-25 39.17368928829677 41.0 39.0 41.0 36.0 41.0 26-27 39.052230972153794 40.5 39.0 41.0 35.0 41.0 28-29 38.911418538646956 40.0 38.0 41.0 35.0 41.0 30-31 38.73825753377572 40.0 38.0 41.0 35.0 41.0 32-33 38.625764656081145 40.0 38.0 41.0 34.5 41.0 34-35 38.594316412477056 40.0 38.0 41.0 35.0 41.0 36-37 38.50656375067561 40.0 38.0 41.0 34.0 41.0 38-39 38.39477858723731 40.0 38.0 41.0 34.0 41.0 40-41 38.24369196877366 40.0 38.0 41.0 34.0 41.0 42-43 38.10012118294475 40.0 38.0 41.0 33.0 41.0 44-45 37.90892286329617 40.0 38.0 41.0 33.0 41.0 46-47 37.652505708829906 40.0 37.5 41.0 33.0 41.0 48-49 37.3809140768003 40.0 37.0 41.0 32.5 41.0 50-51 37.15059934779944 40.0 37.0 41.0 32.0 41.0 52-53 36.924861935909746 40.0 36.0 41.0 31.0 41.0 54-55 36.68866529339016 39.0 36.0 41.0 31.0 41.0 56-57 36.42963406363715 39.0 35.5 41.0 31.0 41.0 58-59 36.133576122370364 39.0 35.0 40.0 30.0 41.0 60-61 35.787094944054196 38.0 35.0 40.0 29.0 41.0 62-63 35.39934931301184 38.0 35.0 40.0 28.5 41.0 64-65 34.99963918534985 37.0 34.0 40.0 28.0 41.0 66-67 34.64546542892757 37.0 34.0 39.5 28.0 41.0 68-69 34.18374229729076 36.0 34.0 39.0 26.5 40.5 70-71 33.75082159790601 36.0 33.5 39.0 26.0 40.0 72-73 33.29304423568081 35.0 33.0 38.0 26.0 40.0 74-75 32.79929209044486 35.0 32.5 37.0 25.5 39.0 76-77 30.821787590026673 33.5 30.0 35.5 21.5 37.5 78-79 31.791907596295765 34.5 31.5 36.0 23.5 38.5 80-81 31.815698171465566 35.0 32.0 36.0 24.0 37.5 82-83 31.532013389690075 35.0 32.0 35.5 23.5 37.0 84-85 31.165159610437357 34.5 31.5 35.0 22.0 37.0 86-87 30.62593676320452 34.0 31.0 35.0 19.0 36.0 88-89 30.101420896880736 34.0 31.0 35.0 15.0 36.0 90-91 29.678141614135342 34.0 30.0 35.0 8.5 35.5 92-93 29.407009178558333 34.0 30.0 35.0 4.5 35.0 94-95 29.103666101439206 34.0 30.0 35.0 2.0 35.0 96-97 28.58492351364138 34.0 29.0 35.0 2.0 35.0 98-99 27.858412230005424 34.0 29.0 35.0 2.0 35.0 100-101 24.284893925863575 30.0 14.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10831827620737755 1101 2 0.1458886217028521 1101 3 0.13764348617385025 1101 4 0.10964492302317552 1101 5 0.117992680570147 1101 6 0.05975642909741907 1101 7 0.07668650920116704 1101 8 0.14701209281552252 1101 9 0.2334657975676464 1101 10-11 0.12584564923329822 1101 12-13 0.17337872126375942 1101 14-15 0.16754054605357993 1101 16-17 0.2115913041002031 1101 18-19 0.21219105129879523 1101 20-21 0.23401467917882002 1101 22-23 0.29252317075935963 1101 24-25 0.24616734702156862 1101 26-27 0.23569203892559898 1101 28-29 0.1348313137177044 1101 30-31 0.2201591204874731 1101 32-33 0.21700190221816484 1101 34-35 0.32534250030683864 1101 36-37 0.32827810175820815 1101 38-39 0.43111032822472595 1101 40-41 0.5254466114506826 1101 42-43 0.3905335708399491 1101 44-45 0.307960522513099 1101 46-47 0.33071457133434023 1101 48-49 0.3550669554294643 1101 50-51 0.20477959925138833 1101 52-53 0.29946802512570514 1101 54-55 0.30877229299735376 1101 56-57 0.412962898720842 1101 58-59 0.4122934006109986 1101 60-61 0.3011765622668321 1101 62-63 0.29783296120856306 1101 64-65 0.07322929677102508 1101 66-67 0.1929490886975671 1101 68-69 0.030843393712395084 1101 70-71 0.09581508568982144 1101 72-73 0.0889596239999122 1101 74-75 0.14088348383079818 1101 76-77 0.14783563866072313 1101 78-79 0.27002890552043013 1101 80-81 0.32514085724198694 1101 82-83 0.28918519221898364 1101 84-85 0.2454627935092688 1101 86-87 0.294154534577455 1101 88-89 0.4096320178688764 1101 90-91 0.5275162062663235 1101 92-93 0.329104466739917 1101 94-95 0.3106686537345382 1101 96-97 0.19685181148763675 1101 98-99 0.22005299691009128 1101 100-101 -0.17764070720551572 1102 1 0.1704817365839162 1102 2 0.12422984992142716 1102 3 0.1657324607249322 1102 4 0.13158132488894836 1102 5 0.13784338265101326 1102 6 0.19167663839243687 1102 7 0.1989618674920024 1102 8 0.19532184219319504 1102 9 0.24718779287905335 1102 10-11 0.20359098480619764 1102 12-13 0.20001927640308992 1102 14-15 0.2131972514696301 1102 16-17 0.27042849021808735 1102 18-19 0.22301016578268218 1102 20-21 0.21335011915756041 1102 22-23 0.2794960059093299 1102 24-25 0.2765625545039967 1102 26-27 0.3076840272282766 1102 28-29 0.15385471439923748 1102 30-31 0.3259228902146134 1102 32-33 0.28001728516038327 1102 34-35 0.3522360643641633 1102 36-37 0.4137301644983822 1102 38-39 0.36051056869649045 1102 40-41 0.38793392324226517 1102 42-43 0.25213708849469185 1102 44-45 0.32282989438635923 1102 46-47 0.3306518855427143 1102 48-49 0.346583830746674 1102 50-51 0.23506786326279183 1102 52-53 0.3463035255244762 1102 54-55 0.17578582104433593 1102 56-57 0.27530399401532435 1102 58-59 0.2803908641777184 1102 60-61 0.2369712066344718 1102 62-63 0.2721636582141471 1102 64-65 0.2940852372693712 1102 66-67 0.374466749475296 1102 68-69 0.17880300895757983 1102 70-71 0.2603610592983472 1102 72-73 0.23928543906625066 1102 74-75 0.1739256932708244 1102 76-77 0.37860333569047455 1102 78-79 0.4063561525417221 1102 80-81 0.25141541798829437 1102 82-83 0.19387650623401242 1102 84-85 0.2539206928253108 1102 86-87 0.26580303495205726 1102 88-89 0.4378519528569065 1102 90-91 0.5274817668434544 1102 92-93 0.3342486289556419 1102 94-95 0.2972620510325541 1102 96-97 0.39089424448181376 1102 98-99 0.31883311630009104 1102 100-101 -0.06200807917545248 1103 1 -0.002057312256276589 1103 2 0.049736902025401264 1103 3 0.07120949767562479 1103 4 0.05024463063553952 1103 5 0.0794347685934298 1103 6 0.014734395706810233 1103 7 0.11424773997131155 1103 8 0.1822273673126702 1103 9 0.1792632095942892 1103 10-11 0.1579439105551188 1103 12-13 0.1423413710528152 1103 14-15 0.23501651944958013 1103 16-17 0.15880490355297638 1103 18-19 0.1754536323871818 1103 20-21 0.16884755475610547 1103 22-23 0.2003696508562811 1103 24-25 0.16971006586895498 1103 26-27 0.1610658316343887 1103 28-29 0.19524721352501473 1103 30-31 0.20636787214723995 1103 32-33 0.25162673847661665 1103 34-35 0.2639478023289712 1103 36-37 0.22276967959337668 1103 38-39 0.18034023998684745 1103 40-41 0.37603131221533914 1103 42-43 0.274959532835517 1103 44-45 0.27899611422550663 1103 46-47 0.15189926285941624 1103 48-49 0.1400118235197212 1103 50-51 0.18677406445173972 1103 52-53 0.18884490563870315 1103 54-55 0.14731440829226727 1103 56-57 0.20190820196497583 1103 58-59 0.29923848627819893 1103 60-61 0.3387284044430743 1103 62-63 0.18219469329981308 1103 64-65 0.17449452156824208 1103 66-67 0.05264966706474894 1103 68-69 0.11304068234608877 1103 70-71 0.05987659141165125 1103 72-73 -0.0389280764949973 1103 74-75 0.0437167528348823 1103 76-77 -0.007994752664810534 1103 78-79 0.2842979289725456 1103 80-81 0.2869571477936397 1103 82-83 0.16997657617223894 1103 84-85 0.12874439022593975 1103 86-87 0.26152514708633845 1103 88-89 0.24950030930871137 1103 90-91 0.2811331248105837 1103 92-93 0.11024233451573195 1103 94-95 -0.005235326284150688 1103 96-97 -0.07638413877964112 1103 98-99 -0.13359432279537486 1103 100-101 -0.30747885082715953 1104 1 0.08881552854869312 1104 2 0.13893357400875317 1104 3 0.12267384762641598 1104 4 0.04510865932299879 1104 5 0.11239621699333213 1104 6 0.09522415261001527 1104 7 0.15222044389999922 1104 8 0.11549933256193157 1104 9 0.06379025114359393 1104 10-11 0.13712726255339902 1104 12-13 0.10454711533171945 1104 14-15 0.18869945290056478 1104 16-17 0.22973297563361683 1104 18-19 0.2544172470521744 1104 20-21 0.24928159961985585 1104 22-23 0.29517202865743997 1104 24-25 0.25684369557902187 1104 26-27 0.2880889987931283 1104 28-29 0.23631320825358415 1104 30-31 0.2766544073674311 1104 32-33 0.26002554052637095 1104 34-35 0.3513853030359684 1104 36-37 0.4063691298258405 1104 38-39 0.40127029168309747 1104 40-41 0.40169745286985403 1104 42-43 0.23967033927435466 1104 44-45 0.2669227213905785 1104 46-47 0.2911809169131061 1104 48-49 0.17568972874703803 1104 50-51 0.20313106267574454 1104 52-53 0.08626179006309087 1104 54-55 0.1668184297399833 1104 56-57 0.14704541760733036 1104 58-59 0.15839604671303675 1104 60-61 0.016908374126678893 1104 62-63 0.04913921315819181 1104 64-65 0.021141052716082243 1104 66-67 0.06346303456059132 1104 68-69 0.0321212074532653 1104 70-71 0.07337587309398685 1104 72-73 -0.0569618091450792 1104 74-75 -0.17510430122559484 1104 76-77 -0.10001068008644509 1104 78-79 0.07005318060996757 1104 80-81 0.08028626737372946 1104 82-83 0.1487265337519048 1104 84-85 0.1462432024565743 1104 86-87 0.2886979166251429 1104 88-89 0.4472940277055635 1104 90-91 0.29571695304782253 1104 92-93 0.22274615234584516 1104 94-95 0.11603154286891026 1104 96-97 0.05740153672176973 1104 98-99 0.10598455640282367 1104 100-101 -0.29400106211436494 1105 1 -0.06661890419584893 1105 2 -0.0015169743830796278 1105 3 -0.03872676853433887 1105 4 0.007963570325649982 1105 5 0.026497463109485864 1105 6 -0.0017590354416370246 1105 7 0.02861852009460364 1105 8 0.07063800157587963 1105 9 0.06256707132493489 1105 10-11 0.023311461555373114 1105 12-13 -0.038154642281064355 1105 14-15 0.09820576887673127 1105 16-17 -0.003385418538478291 1105 18-19 0.028297444950574402 1105 20-21 -0.02679027704289183 1105 22-23 -0.021742120486742067 1105 24-25 -0.03350180550593507 1105 26-27 0.03427384920883014 1105 28-29 -0.05217247925613577 1105 30-31 -0.005865370429326333 1105 32-33 -0.019079926093880317 1105 34-35 0.05329030501758325 1105 36-37 0.06589843687491026 1105 38-39 0.03387773663042282 1105 40-41 -0.07709478775252165 1105 42-43 0.12144805282311211 1105 44-45 0.20152735157370216 1105 46-47 0.1273305198244259 1105 48-49 0.13445428154876993 1105 50-51 0.0588683754353454 1105 52-53 0.12501762267932293 1105 54-55 0.17375927042498773 1105 56-57 0.17749736735979127 1105 58-59 0.26806332756979145 1105 60-61 0.270829310016623 1105 62-63 0.165305030249975 1105 64-65 0.0906770052800212 1105 66-67 0.0461651260913456 1105 68-69 0.08328113445114127 1105 70-71 0.18192603414097164 1105 72-73 0.07659661774002302 1105 74-75 0.09417371339672798 1105 76-77 0.04212232217543743 1105 78-79 0.09804152742045247 1105 80-81 -0.039818139467538316 1105 82-83 0.043572756318624783 1105 84-85 0.04356301813385954 1105 86-87 0.2026456152187457 1105 88-89 0.26404819730085194 1105 90-91 0.20915693211499686 1105 92-93 0.14558975393025975 1105 94-95 0.04891307424953695 1105 96-97 0.08542970735494038 1105 98-99 0.08357387124695492 1105 100-101 -0.29367097599637404 1106 1 -0.013308397619127277 1106 2 0.07168847615823637 1106 3 0.09501885972005653 1106 4 -0.019400131291220646 1106 5 -0.03389437049471411 1106 6 -0.03124558334008043 1106 7 0.03196915059652383 1106 8 0.013648876724587922 1106 9 -0.013437716717895398 1106 10-11 0.09073688379030642 1106 12-13 0.08707014244244249 1106 14-15 0.008273994015745245 1106 16-17 -0.015748348187337058 1106 18-19 -0.0868663740264779 1106 20-21 0.07228294050663919 1106 22-23 0.04176875321534368 1106 24-25 0.13151996872642968 1106 26-27 0.12991595168672632 1106 28-29 0.15778177868007504 1106 30-31 0.10140136043211356 1106 32-33 -0.050740609460476094 1106 34-35 -0.04716509035497296 1106 36-37 0.027125126925930942 1106 38-39 -0.03043416895749118 1106 40-41 -0.11831814269131513 1106 42-43 -0.14076725602591011 1106 44-45 -0.17932418569473896 1106 46-47 -0.24916959025004815 1106 48-49 -0.20044411720282795 1106 50-51 -0.1326774043720178 1106 52-53 -0.10559355241906587 1106 54-55 -0.1980447228195672 1106 56-57 -0.3031543788002935 1106 58-59 -0.29143765231670216 1106 60-61 -0.4959256178878775 1106 62-63 -0.3650152957072734 1106 64-65 -0.22834515454182025 1106 66-67 -0.3312224184217527 1106 68-69 -0.4075227143946307 1106 70-71 -0.3396423839242857 1106 72-73 -0.4441605106020958 1106 74-75 -0.47346764946480135 1106 76-77 -0.29496428369294136 1106 78-79 -0.1722030378326771 1106 80-81 -0.2218648988983709 1106 82-83 -0.2492610133234905 1106 84-85 -0.25567598184799323 1106 86-87 -0.2247719005908131 1106 88-89 0.03967286671574399 1106 90-91 0.1500048502080098 1106 92-93 -0.005004109095001041 1106 94-95 -0.19159678234852606 1106 96-97 -0.11764233080631925 1106 98-99 -0.11547735770771084 1106 100-101 -0.3305594740938069 1107 1 0.06237862289502516 1107 2 0.022883419325289367 1107 3 0.08812082145930589 1107 4 0.07497727360465944 1107 5 0.09913650113690409 1107 6 0.09142425079486571 1107 7 0.08426287922284814 1107 8 0.035244327039038126 1107 9 0.09423654783958568 1107 10-11 0.11869035901578684 1107 12-13 0.07882226144516835 1107 14-15 0.1669427270267434 1107 16-17 0.14107221487031296 1107 18-19 0.2102877594332 1107 20-21 0.23396031932326622 1107 22-23 0.14383140340616052 1107 24-25 0.08269889831804278 1107 26-27 0.16542300503125773 1107 28-29 0.14197587134835032 1107 30-31 0.18041844208762114 1107 32-33 0.03685682552059433 1107 34-35 0.08001396327830435 1107 36-37 0.12881018717510528 1107 38-39 0.05447806902355268 1107 40-41 0.095124516761949 1107 42-43 0.009345398206434652 1107 44-45 0.05928927252076477 1107 46-47 0.02859489936679438 1107 48-49 -0.06667687528446464 1107 50-51 -0.15736868551397976 1107 52-53 -0.14723088204605972 1107 54-55 -0.128693104863828 1107 56-57 -0.11047344700337902 1107 58-59 -0.25814021819985555 1107 60-61 -0.024328998328243756 1107 62-63 -0.08771570814580798 1107 64-65 -0.20165497249033848 1107 66-67 -0.0717836866155892 1107 68-69 -0.24693616508628935 1107 70-71 -0.18776620292310042 1107 72-73 -0.203797886998899 1107 74-75 -0.10270038060296827 1107 76-77 -0.17756189914446807 1107 78-79 -0.02831470786558654 1107 80-81 -0.008686082164334863 1107 82-83 -0.08942938971470227 1107 84-85 0.029971126735738096 1107 86-87 -0.015854982578797916 1107 88-89 0.09280730805293302 1107 90-91 0.22975271010803056 1107 92-93 0.16154355721868896 1107 94-95 0.15643413249432925 1107 96-97 0.020743302525509932 1107 98-99 0.21393472547544334 1107 100-101 -0.03843055311902077 1108 1 0.052560679506825636 1108 2 0.04113073046706717 1108 3 0.04504306950504855 1108 4 0.010323379917771547 1108 5 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-0.43105849174367705 2316 70-71 -0.3021915353720601 2316 72-73 -0.08745895083556832 2316 74-75 -0.3406693277670385 2316 76-77 -0.3190737330332283 2316 78-79 -0.31959055152559657 2316 80-81 -0.18172799014634577 2316 82-83 -0.129905050568226 2316 84-85 -0.275602448829666 2316 86-87 -0.4178838514434524 2316 88-89 -0.5487451719677772 2316 90-91 -0.7027075739700095 2316 92-93 -0.6947660975520726 2316 94-95 -0.2188406838903063 2316 96-97 -0.5987183081128578 2316 98-99 -0.47076190473777757 2316 100-101 -0.10695022503800544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 5.0 5 33.0 6 59.0 7 154.0 8 357.0 9 721.0 10 1262.0 11 2008.0 12 2918.0 13 4416.0 14 5264.0 15 7254.0 16 9499.0 17 13550.0 18 13460.0 19 9419.0 20 8574.0 21 9228.0 22 9700.0 23 10705.0 24 12467.0 25 14677.0 26 17424.0 27 21184.0 28 25870.0 29 31922.0 30 39237.0 31 48281.0 32 60718.0 33 79523.0 34 110604.0 35 163388.0 36 274018.0 37 494626.0 38 491599.0 39 54009.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33303773094957 19.445231084305473 14.950980632327795 23.270750552417162 2 15.555456821130164 20.24760966397366 40.2485568634612 23.948376651434984 3 19.391614745650084 24.101686259211395 29.61258076218311 26.894118232955417 4 13.403019222778878 16.91898466073902 36.43642069914064 33.241575417341465 5 14.268583785409513 36.869886526477885 33.98307637699126 14.878453311121342 6 32.49157895713336 35.5335540535988 17.374372785493982 14.60049420377386 7 29.00075824783719 32.12983663738323 20.545586904820613 18.323818209958972 8 26.02513594021511 35.779972394505656 19.553566328132362 18.64132533714687 9 26.127374895209954 17.469385682542672 20.503304700892468 35.8999347213549 10-11 25.202341828671145 26.706558087008574 26.90195459984972 21.18914548447057 12-13 25.920382512353875 23.73845478562776 27.723161908128485 22.61800079388988 14-15 22.707497351835208 25.248832280780903 25.877019202765076 26.16665116461881 16-17 22.53674927731853 28.498148163067118 26.51592218452064 22.449180375093714 18-19 22.423957909168408 27.12485267134798 28.634855395768454 21.816334023715154 20-21 23.01523868206468 26.37018020714374 28.715548670185626 21.899032440605954 22-23 22.806293310574503 26.216650888145992 28.593999540071174 22.383056261208324 24-25 22.821311670709367 26.349568583336264 28.563142291332216 22.265977454622153 26-27 22.429474416074825 26.71369186015286 28.494835756854325 22.361997966917986 28-29 22.332302969079795 26.848345061843826 28.62029164955767 22.199060319518704 30-31 22.486057659913346 26.482853680377406 28.492957787740508 22.538130871968743 32-33 22.286065963167612 26.547999822278033 28.477064345604774 22.68886986894958 34-35 22.88534341699655 26.659343835955575 28.253536614723235 22.201776132324643 36-37 22.82083303012351 26.328754987089365 28.29624080053254 22.55417118225458 38-39 22.45994214469004 26.673607806242583 28.47370542822672 22.392744620840656 40-41 22.72949123971696 26.491972924781376 28.336077267359116 22.44245856814255 42-43 22.540766173835575 26.54793536593911 28.108975431039006 22.802323029186308 44-45 22.67421875839198 26.702369920013126 28.02914526050176 22.594266061093137 46-47 22.78015936566217 26.909922270179482 27.635294806944906 22.674623557213444 48-49 22.808969261450148 26.936981737229864 27.600807273567064 22.65324172775292 50-51 22.672402597085554 27.055912138904432 27.579264523651194 22.692420740358823 52-53 22.983725969971296 26.984623369344046 27.40681120264943 22.62483945803523 54-55 22.651372487174967 26.945628308179653 27.523981479808786 22.879017724836594 56-57 22.70249196467239 27.248024185811243 27.376067003134057 22.673416846382306 58-59 22.757319031606148 26.86888656581306 27.433423288775522 22.940371113805273 60-61 22.7966695305208 26.83704767645724 27.75633752852562 22.60994526449634 62-63 22.668559837133955 26.937177484975443 27.70148484165652 22.692777836234082 64-65 22.507087035534294 26.84885450264781 27.56029228955923 23.083766172258663 66-67 22.46320607891605 27.0793982117245 27.368696023680915 23.088699685678538 68-69 22.558806411480823 26.892214937336313 27.591944830172316 22.957033821010548 70-71 22.4821270980901 26.904719764371865 27.532263990420585 23.08088914711745 72-73 22.59683527956788 26.822150601789037 27.465220934280467 23.115793184362616 74-75 22.47452936635772 27.06075698815952 27.21465249252616 23.250061152956604 76-77 22.5097172070715 26.902259343920015 27.41349685626607 23.174526592742414 78-79 22.550968295074156 26.916816070807798 27.333121433342317 23.19909420077573 80-81 22.36830284121148 27.089039925393177 27.364445551177454 23.178211682217892 82-83 22.518893421742284 26.981956986690562 27.278470861396485 23.220678730170665 84-85 22.4115753664601 26.863267570493754 27.28750563010142 23.43765143294473 86-87 22.433505516441794 26.83426821853047 27.381195610308307 23.351030654719427 88-89 22.313825934317418 26.801500859535498 27.292268298619764 23.592404907527314 90-91 22.219185743987957 26.865708223417588 27.407543935457163 23.507562097137296 92-93 22.299590409458713 26.672161074690585 27.385685472156972 23.642563043693727 94-95 22.15973201089963 26.534646262492412 27.380436815202845 23.92518491140511 96-97 22.21783342276394 26.431015803095782 27.408999273976477 23.942151500163806 98-99 22.00618909954851 26.490161450737432 27.29570625115655 24.207943198557505 100-101 22.14338061713886 25.93872260975749 27.803961566873962 24.113935206229687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 227.0 1 165.0 2 135.5 3 187.0 4 168.5 5 157.0 6 298.0 7 661.0 8 1101.0 9 1359.0 10 1340.5 11 1312.5 12 1463.0 13 1626.5 14 1775.0 15 1906.5 16 2001.5 17 2105.5 18 2264.5 19 2476.5 20 2715.5 21 3112.5 22 3748.0 23 4638.0 24 5799.0 25 7331.5 26 9572.0 27 12364.5 28 14830.5 29 17782.5 30 21337.0 31 24829.0 32 29023.5 33 32846.5 34 36753.5 35 40555.5 36 44776.5 37 49972.0 38 53227.5 39 55276.5 40 57772.5 41 61859.0 42 66052.5 43 69765.5 44 73867.0 45 76413.5 46 77389.0 47 79902.0 48 85641.0 49 87090.5 50 83722.0 51 81164.5 52 77753.0 53 75652.0 54 73786.0 55 67829.5 56 60919.0 57 54259.0 58 47723.5 59 41844.0 60 35358.0 61 28643.0 62 22757.5 63 17102.5 64 12671.0 65 9642.5 66 7125.0 67 5691.5 68 4246.5 69 2982.5 70 2200.5 71 1610.0 72 1304.5 73 1056.5 74 788.0 75 604.5 76 395.5 77 202.5 78 106.0 79 62.5 80 32.5 81 19.0 82 10.5 83 6.5 84 4.0 85 1.0 86 0.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3472902038578361 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012938549700875377 16-17 0.002026225707872937 18-19 4.882471585235991E-5 20-21 0.0 22-23 0.0 24-25 3.4177301096651944E-4 26-27 0.0010253190328995583 28-29 0.0 30-31 0.0031736065304033946 32-33 0.0 34-35 0.010253190328995583 36-37 0.02958777780653011 38-39 0.021897885059783424 40-41 0.014281229386815277 42-43 0.010424076834478841 44-45 0.0027830088035845153 46-47 0.001122968464604278 48-49 7.079583798592188E-4 50-51 4.882471585235991E-5 52-53 1.2206178963089979E-4 54-55 0.0 56-57 0.0 58-59 0.004491873858417112 60-61 0.009935829675955243 62-63 0.0038815649102626137 64-65 0.0018065144865373171 66-67 0.0039303896261149735 68-69 0.0032712559621081144 70-71 2.4412357926179956E-5 72-73 0.0 74-75 0.0 76-77 0.21846619108138446 78-79 3.4177301096651944E-4 80-81 0.003564204257222274 82-83 4.6383480059741924E-4 84-85 2.4412357926179956E-5 86-87 0.11029503311048106 88-89 0.40907788176899756 90-91 0.3971158263851694 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0012694426121613578 100-101 6.835460219330389E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2048143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.36077857480147 #Duplication Level Percentage of deduplicated Percentage of total 1 78.13648962486639 45.601063696090996 2 12.772572756422814 14.908345809362672 3 3.8890966843357946 6.809121313515478 4 1.733034270243692 4.045649172329391 5 0.9333041924490377 2.72341796592261 6 0.5374144342166909 1.8818354878933508 7 0.36920817196394023 1.508309346039632 8 0.2570808381960117 1.2002750299025442 9 0.20168603709253397 1.0593498737147913 >10 1.048907690288194 11.168682977159214 >50 0.0763465294847326 3.0937977892902557 >100 0.04209312262716901 4.435693407450296 >500 0.002346610265688395 0.8466933880895656 >1k 3.352300379554811E-4 0.46841791795584836 >5k 8.380750948887027E-5 0.24934682528337843 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5098 0.24890840141533085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4375 0.21360813185407465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2772 0.1353421123427417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.882471585235992E-5 2 0.0 0.0 0.0 0.0 4.882471585235992E-5 3 0.0 0.0 0.0 0.0 4.882471585235992E-5 4 0.0 0.0 0.0 0.0 4.882471585235992E-5 5 0.0 0.0 0.0 0.0 4.882471585235992E-5 6 0.0 0.0 0.0 0.0 4.882471585235992E-5 7 0.0 0.0 0.0 0.0 4.882471585235992E-5 8 0.0 0.0 0.0 0.0 4.882471585235992E-5 9 0.0 0.0 0.0 0.0 4.882471585235992E-5 10-11 0.0 0.0 0.0 0.0 4.882471585235992E-5 12-13 0.0 0.0 0.0 0.0 4.882471585235992E-5 14-15 0.0 0.0 0.0 0.0 4.882471585235992E-5 16-17 0.0 0.0 0.0 0.0 4.882471585235992E-5 18-19 2.441235792617996E-5 0.0 0.0 0.0 4.882471585235992E-5 20-21 4.882471585235992E-5 0.0 0.0 7.323707377853988E-5 9.764943170471984E-5 22-23 4.882471585235992E-5 0.0 0.0 9.764943170471984E-5 9.764943170471984E-5 24-25 4.882471585235992E-5 0.0 0.0 1.220617896308998E-4 1.220617896308998E-4 26-27 4.882471585235992E-5 0.0 0.0 5.370718743759591E-4 1.4647414755707976E-4 28-29 4.882471585235992E-5 0.0 0.0 0.002685359371879795 1.4647414755707976E-4 30-31 4.882471585235992E-5 0.0 0.0 0.007616655672968147 1.4647414755707976E-4 32-33 4.882471585235992E-5 0.0 0.0 0.017039825832473612 1.4647414755707976E-4 34-35 4.882471585235992E-5 0.0 0.0 0.033127569705826206 1.7088650548325972E-4 36-37 4.882471585235992E-5 0.0 0.0 0.059639390413657636 1.9529886340943967E-4 38-39 4.882471585235992E-5 0.0 0.0 0.09901652374858591 1.9529886340943967E-4 40-41 4.882471585235992E-5 0.0 0.0 0.1443502724175021 1.9529886340943967E-4 42-43 4.882471585235992E-5 0.0 0.0 0.19056286597176075 1.9529886340943967E-4 44-45 4.882471585235992E-5 0.0 0.0 0.24907928792081413 1.9529886340943967E-4 46-47 4.882471585235992E-5 0.0 0.0 0.31894745630554117 1.9529886340943967E-4 48-49 4.882471585235992E-5 0.0 0.0 0.3982143824918475 1.9529886340943967E-4 50-51 4.882471585235992E-5 0.0 0.0 0.4810699252933023 1.9529886340943967E-4 52-53 4.882471585235992E-5 0.0 0.0 0.5646578388325425 1.9529886340943967E-4 54-55 4.882471585235992E-5 0.0 0.0 0.6629175794854167 1.9529886340943967E-4 56-57 4.882471585235992E-5 0.0 0.0 0.7773138887274961 1.9529886340943967E-4 58-59 4.882471585235992E-5 0.0 0.0 0.9112889090263717 1.9529886340943967E-4 60-61 4.882471585235992E-5 0.0 0.0 1.0603263541657002 1.9529886340943967E-4 62-63 4.882471585235992E-5 0.0 0.0 1.2109994272860831 2.1971122133561963E-4 64-65 4.882471585235992E-5 0.0 0.0 1.3737322052219987 2.4412357926179959E-4 66-67 4.882471585235992E-5 0.0 0.0 1.5660527609644443 2.6853593718797954E-4 68-69 4.882471585235992E-5 0.0 0.0 1.7861545800268828 2.929482951141595E-4 70-71 4.882471585235992E-5 0.0 0.0 2.0280078100015477 2.929482951141595E-4 72-73 4.882471585235992E-5 0.0 0.0 2.277135922638214 3.173606530403395E-4 74-75 4.882471585235992E-5 0.0 0.0 2.536443988530098 3.4177301096651944E-4 76-77 4.882471585235992E-5 0.0 0.0 2.8258769041028873 3.4177301096651944E-4 78-79 4.882471585235992E-5 0.0 0.0 3.156176106844102 3.4177301096651944E-4 80-81 4.882471585235992E-5 0.0 0.0 3.522190589231318 3.4177301096651944E-4 82-83 4.882471585235992E-5 0.0 0.0 3.900899497740148 3.4177301096651944E-4 84-85 4.882471585235992E-5 0.0 0.0 4.289178050556041 3.4177301096651944E-4 86-87 4.882471585235992E-5 0.0 0.0 4.691420472105707 3.4177301096651944E-4 88-89 4.882471585235992E-5 0.0 0.0 5.1407299197370495 3.4177301096651944E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4725 0.0 38.69779 1 GTATCAA 7295 0.0 32.70161 1 ATCAACG 8705 0.0 27.251223 3 TCAACGC 8820 0.0 26.895905 4 CAACGCA 8940 0.0 26.481815 5 TATCAAC 9035 0.0 26.360903 2 AACGCAG 9195 0.0 25.799007 6 GTACATG 7145 0.0 23.724905 1 TACATGG 7220 0.0 22.933638 2 ACGCAGA 10375 0.0 22.68184 7 GTATAGG 420 0.0 22.674484 1 CGCAGAG 10525 0.0 22.268429 8 ACATGGG 7250 0.0 21.52993 3 GAGTACT 5560 0.0 20.907265 12-13 GCAGAGT 11590 0.0 19.97658 9 CATGGGG 4245 0.0 19.55893 4 GTACTTT 6120 0.0 19.149239 14-15 AGAGTAC 9695 0.0 18.743767 10-11 AGTACTT 5990 0.0 18.257868 12-13 GTACCTG 1900 0.0 18.044117 1 >>END_MODULE