FastQCFastQC Report
Fri 27 May 2016
SRR939138_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939138_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015105
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50620.2512027909215649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.1977564444532667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24190.12004337242972452No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.034.1881181
GTATCAA72650.033.3370631
ATCAACG87350.027.1617183
TCAACGC88650.026.8168264
TATCAAC90700.026.367352
CAACGCA90000.026.3619545
AACGCAG93300.025.5164686
ACGCAGA104550.022.5897147
CGCAGAG105850.022.088168
GTACATG73700.021.1348481
TACCTGG17500.021.1076032
TACATGG73400.020.516982
GTACCTG18800.020.4606741
ACATGGG74450.020.0367953
GAGTACT54700.019.7714512-13
GCAGAGT115300.019.5799929
AGTACTT59400.019.16321212-13
GTATAGG4550.018.7868461
AGAGTAC95450.018.76803210-11
GTACTTT59000.018.3717314-15
Produced by FastQC (version 0.11.5)