##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939138_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015105 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00147932737996 33.0 31.0 34.0 30.0 34.0 2 32.12007562881339 34.0 31.0 34.0 30.0 34.0 3 32.18038712622916 34.0 31.0 34.0 30.0 34.0 4 35.615799176717836 37.0 35.0 37.0 33.0 37.0 5 35.64454358457748 37.0 35.0 37.0 35.0 37.0 6 35.72245069115505 37.0 36.0 37.0 35.0 37.0 7 35.64854387240367 37.0 36.0 37.0 33.0 37.0 8 35.638168730661675 37.0 36.0 37.0 33.0 37.0 9 37.30088903555894 39.0 38.0 39.0 34.0 39.0 10-11 37.19602700603691 39.0 37.0 39.0 34.0 39.0 12-13 37.09595777887505 39.0 37.0 39.0 33.5 39.0 14-15 38.38339218055634 40.0 38.0 41.0 34.0 41.0 16-17 38.28934422772014 40.0 38.0 41.0 33.0 41.0 18-19 38.17075636257168 40.0 38.0 41.0 33.0 41.0 20-21 38.14961726560155 40.0 38.0 41.0 33.5 41.0 22-23 38.189389634783296 40.0 38.0 41.0 33.5 41.0 24-25 38.13589093372306 40.0 38.0 41.0 33.0 41.0 26-27 38.02709908416683 40.0 38.0 41.0 33.0 41.0 28-29 37.884161867495735 40.0 38.0 41.0 33.0 41.0 30-31 37.6821922430841 40.0 38.0 41.0 32.5 41.0 32-33 37.490397522709735 40.0 37.5 41.0 32.0 41.0 34-35 37.18969855168837 40.0 37.0 41.0 31.0 41.0 36-37 36.932570014961996 40.0 37.0 41.0 30.0 41.0 38-39 36.754221740306335 40.0 36.5 41.0 30.0 41.0 40-41 36.57103724123557 40.0 36.0 41.0 30.0 41.0 42-43 36.371662766952596 40.0 36.0 41.0 30.0 41.0 44-45 36.11534510608628 39.0 36.0 41.0 29.0 41.0 46-47 36.00864123705712 39.5 36.0 41.0 28.0 41.0 48-49 35.911039871371464 40.0 36.0 41.0 27.5 41.0 50-51 34.808778698876736 38.5 34.0 40.0 25.5 40.5 52-53 34.95996188784207 38.5 34.5 39.5 26.0 40.5 54-55 35.485245930112825 39.0 35.0 41.0 27.0 41.0 56-57 35.40001736882197 39.0 35.0 41.0 26.0 41.0 58-59 35.196486287315054 39.0 35.0 41.0 26.0 41.0 60-61 34.90252542671474 38.5 35.0 40.5 26.0 41.0 62-63 34.52080511933622 38.0 34.0 40.0 25.0 41.0 64-65 34.14154597403113 37.5 34.0 40.0 23.5 41.0 66-67 33.751042501507364 37.0 34.0 40.0 22.5 41.0 68-69 33.3389562826751 36.0 33.5 39.0 22.0 41.0 70-71 32.912261892060215 36.0 33.0 39.0 20.5 41.0 72-73 32.462206684018945 35.0 32.5 38.5 19.5 40.0 74-75 32.00501884517184 35.0 32.0 37.5 18.0 39.5 76-77 31.56262973889698 35.0 32.0 37.0 15.5 39.0 78-79 31.125217296369172 35.0 31.0 36.5 11.5 39.0 80-81 30.670884643728243 35.0 31.0 36.0 7.0 37.5 82-83 30.282241372037685 35.0 31.0 35.5 4.5 37.0 84-85 29.894659831621677 34.0 30.5 35.0 2.0 37.0 86-87 29.48029804898504 34.0 30.0 35.0 2.0 36.0 88-89 29.061769237831278 34.0 29.5 35.0 2.0 36.0 90-91 28.659057716595413 34.0 29.0 35.0 2.0 35.5 92-93 28.23650976003732 34.0 29.0 35.0 2.0 35.0 94-95 27.748873631895112 34.0 28.0 35.0 2.0 35.0 96-97 27.14470362586565 34.0 26.5 35.0 2.0 35.0 98-99 26.419917076281386 33.5 25.0 35.0 2.0 35.0 100-101 23.525415549065684 30.0 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38439424890951557 1101 2 0.5054090880829989 1101 3 0.4209743887664814 1101 4 0.5303288395082717 1101 5 0.3534614971992909 1101 6 0.33977711456768134 1101 7 0.386422476667974 1101 8 0.39861612845349725 1101 9 0.5092008176279919 1101 10-11 0.5594875584216226 1101 12-13 0.6132494063500431 1101 14-15 0.7938163548222903 1101 16-17 0.7446067276093231 1101 18-19 0.8930800838640565 1101 20-21 0.8376638780441255 1101 22-23 0.8764001950151865 1101 24-25 0.9938393474665546 1101 26-27 0.8908646216178511 1101 28-29 0.8618185814298442 1101 30-31 0.8366318933120951 1101 32-33 0.905615884608352 1101 34-35 0.8681929903853032 1101 36-37 0.9009068842997152 1101 38-39 0.7729632711388561 1101 40-41 0.7949483946659512 1101 42-43 0.6290261809693192 1101 44-45 0.7694968874053885 1101 46-47 0.8729340511646058 1101 48-49 0.7769704164810278 1101 50-51 0.7207938536214655 1101 52-53 0.7382605404966256 1101 54-55 0.7045818656608347 1101 56-57 0.6793660909171493 1101 58-59 0.6065391604984072 1101 60-61 0.4730396377270125 1101 62-63 0.45339421314012895 1101 64-65 0.3435907999879646 1101 66-67 0.42499896167782936 1101 68-69 0.4984897428214623 1101 70-71 0.2704239828181372 1101 72-73 0.3724428066929093 1101 74-75 0.3194300208721401 1101 76-77 0.3680128784835084 1101 78-79 0.41515675541490893 1101 80-81 0.28351061344908857 1101 82-83 0.29539819581145466 1101 84-85 0.23244580001517434 1101 86-87 0.08388027224437522 1101 88-89 0.21936534785622896 1101 90-91 0.22961753358867298 1101 92-93 0.05099558111982461 1101 94-95 -0.037394893454031575 1101 96-97 -0.21248483615266167 1101 98-99 -0.5007742578415311 1101 100-101 -0.6265605760422623 1102 1 0.24856291361266614 1102 2 0.2771793977120325 1102 3 0.3495423552636723 1102 4 0.22115805664516586 1102 5 0.2918123412844622 1102 6 0.2749872278530461 1102 7 0.2960100932690608 1102 8 0.388760232572551 1102 9 0.4092183168395849 1102 10-11 0.40024363137193575 1102 12-13 0.5353538376162206 1102 14-15 0.5257824101286062 1102 16-17 0.530735203295805 1102 18-19 0.47565055438511905 1102 20-21 0.4849520038219097 1102 22-23 0.6181462953032906 1102 24-25 0.6510190914367229 1102 26-27 0.6485002263446518 1102 28-29 0.601245091980033 1102 30-31 0.6368069483748116 1102 32-33 0.6728312061123063 1102 34-35 0.650552368798202 1102 36-37 0.6412593423409518 1102 38-39 0.5960039102251713 1102 40-41 0.6269119006267019 1102 42-43 0.594631575006872 1102 44-45 0.6004991956647032 1102 46-47 0.6983304744012955 1102 48-49 0.5056547401459426 1102 50-51 0.3791883135473242 1102 52-53 0.5349429921235824 1102 54-55 0.5642600627132879 1102 56-57 0.532063330007368 1102 58-59 0.47572641654019066 1102 60-61 0.4800961318543884 1102 62-63 0.4076440036846378 1102 64-65 0.3834334518612934 1102 66-67 0.41491519836537094 1102 68-69 0.37175500757886937 1102 70-71 0.1861936050680697 1102 72-73 0.2624860700314926 1102 74-75 0.22275553201593112 1102 76-77 0.12946085641183913 1102 78-79 0.048184440048899546 1102 80-81 0.16242299338592758 1102 82-83 -0.25856735843830236 1102 84-85 -0.1441238572331791 1102 86-87 -0.25014238105154973 1102 88-89 -0.2970219084373511 1102 90-91 -0.3214390914680578 1102 92-93 -0.20998659045679702 1102 94-95 -0.3182832050507862 1102 96-97 -0.1807741245579244 1102 98-99 -0.30729324920711676 1102 100-101 -0.33784750457182966 1103 1 0.3568182361121188 1103 2 0.3214644304114387 1103 3 0.3293162480441225 1103 4 0.3543659102513175 1103 5 0.3968778666355206 1103 6 0.3620406632823787 1103 7 0.372564912381101 1103 8 0.30655320164598265 1103 9 0.31820302149824187 1103 10-11 0.3631108806319716 1103 12-13 0.34905237444636583 1103 14-15 0.42287544228559426 1103 16-17 0.45678183888624346 1103 18-19 0.451222313572778 1103 20-21 0.41531450463037345 1103 22-23 0.34739738192460834 1103 24-25 0.4601488351624994 1103 26-27 0.4821936577496402 1103 28-29 0.5149060723778973 1103 30-31 0.4341482841652464 1103 32-33 0.4772195283739933 1103 34-35 0.39731953172844214 1103 36-37 0.42991786219883465 1103 38-39 0.5096750452324272 1103 40-41 0.3086492574349933 1103 42-43 0.2004002680024115 1103 44-45 0.3374610677622414 1103 46-47 0.3183267125090765 1103 48-49 0.36916382320971053 1103 50-51 0.508693952792477 1103 52-53 0.5532447227502217 1103 54-55 0.6187318871091563 1103 56-57 0.554420967851506 1103 58-59 0.5243435618326586 1103 60-61 0.6683947723552848 1103 62-63 0.5414069238628443 1103 64-65 0.49521836036200995 1103 66-67 0.6062288205082851 1103 68-69 0.43063971276868784 1103 70-71 0.3614807539880758 1103 72-73 0.40173091703589847 1103 74-75 0.2671100847334955 1103 76-77 0.34445049714801 1103 78-79 0.49716346437528003 1103 80-81 0.35926056681546825 1103 82-83 0.3337272614621689 1103 84-85 0.37450860121025187 1103 86-87 0.3148697716725266 1103 88-89 0.21684085186043944 1103 90-91 0.12582099182473883 1103 92-93 0.12544952814529253 1103 94-95 0.14907347513556957 1103 96-97 0.23488606409284785 1103 98-99 0.1392938178198655 1103 100-101 -0.10527554822852281 1104 1 0.25299348231633445 1104 2 0.347491796913296 1104 3 0.3906218825616605 1104 4 0.2990227661722642 1104 5 0.4174750437633392 1104 6 0.3239404212320949 1104 7 0.29876547132715103 1104 8 0.22922165262892946 1104 9 0.42762921112974084 1104 10-11 0.4043493937216951 1104 12-13 0.34179595532364715 1104 14-15 0.4790132956872455 1104 16-17 0.3262932326138923 1104 18-19 0.44286551618099423 1104 20-21 0.4889440464413042 1104 22-23 0.6277868179339734 1104 24-25 0.4696259197214303 1104 26-27 0.5041711352473399 1104 28-29 0.6170107273177763 1104 30-31 0.5738029171753993 1104 32-33 0.6198070564223315 1104 34-35 0.6735924662551156 1104 36-37 0.56807139423195 1104 38-39 0.3434610440378876 1104 40-41 0.33835228553348173 1104 42-43 0.4389579656356446 1104 44-45 0.43053935763280293 1104 46-47 0.6497023874282064 1104 48-49 0.5594740277923549 1104 50-51 0.5225768914655902 1104 52-53 0.5862452930727926 1104 54-55 0.3714457094229928 1104 56-57 0.5209798570248054 1104 58-59 0.5022301649405421 1104 60-61 0.30890893087502036 1104 62-63 0.33276631240520516 1104 64-65 0.2455149730052142 1104 66-67 0.20300238149163619 1104 68-69 0.3110412737460564 1104 70-71 0.29480809968288213 1104 72-73 0.3706933940022097 1104 74-75 0.27493278476927685 1104 76-77 0.1897320134575864 1104 78-79 0.21594204599353262 1104 80-81 0.19591030343422133 1104 82-83 0.1538151899338409 1104 84-85 0.12664252073339455 1104 86-87 0.11702487246693849 1104 88-89 0.0043989880881945 1104 90-91 0.2030031492607094 1104 92-93 0.10553015169796609 1104 94-95 0.0957641821708961 1104 96-97 -0.08829462936551025 1104 98-99 -0.08124497983238044 1104 100-101 -0.38947791244759244 1105 1 0.21139099374316572 1105 2 0.20028261901902056 1105 3 0.2163389411273755 1105 4 0.1616836383112883 1105 5 0.24519855713685956 1105 6 0.18575317488497944 1105 7 0.17693353539709733 1105 8 0.2206895237376898 1105 9 0.26062301246510344 1105 10-11 0.30059376568711826 1105 12-13 0.301198544077252 1105 14-15 0.28889031850675906 1105 16-17 0.322574887640279 1105 18-19 0.37203524233548535 1105 20-21 0.24239443720194487 1105 22-23 0.42667914103865456 1105 24-25 0.39852214189431834 1105 26-27 0.37581694047607783 1105 28-29 0.23620808847394414 1105 30-31 0.3311034243388704 1105 32-33 0.4406748979323254 1105 34-35 0.365868042150332 1105 36-37 0.4746296554182976 1105 38-39 0.6219255226727043 1105 40-41 0.4215225410505212 1105 42-43 0.405854734247292 1105 44-45 0.47777827659479755 1105 46-47 0.4415080829138205 1105 48-49 0.39423521697968766 1105 50-51 0.3321641879029258 1105 52-53 0.4925471264886596 1105 54-55 0.5389451259857765 1105 56-57 0.4914748679251275 1105 58-59 0.48396829555078824 1105 60-61 0.36432621655434616 1105 62-63 0.3692590294011566 1105 64-65 0.4189372733985337 1105 66-67 0.403084205003303 1105 68-69 0.36981275419663007 1105 70-71 0.2798255743389433 1105 72-73 0.3699820275068717 1105 74-75 0.3837273181048886 1105 76-77 0.4383124111056276 1105 78-79 0.25980585182562876 1105 80-81 0.2879539274615901 1105 82-83 0.3869431818283324 1105 84-85 0.3954808545505557 1105 86-87 0.3441870053995473 1105 88-89 0.381631987297002 1105 90-91 0.27020800118150134 1105 92-93 0.32468837987029886 1105 94-95 0.2692266116603754 1105 96-97 0.1967335179225067 1105 98-99 -0.0215008668561687 1105 100-101 -0.11405129177285644 1106 1 0.2320245549123925 1106 2 0.23491506041087007 1106 3 0.30191903569866696 1106 4 0.35191967851240946 1106 5 0.3333347375873217 1106 6 0.21399845047771748 1106 7 0.2879212123025212 1106 8 0.35823837541153125 1106 9 0.3114279217465068 1106 10-11 0.30042524473564214 1106 12-13 0.36130190174634436 1106 14-15 0.3841092294401065 1106 16-17 0.40545310353557795 1106 18-19 0.49077779332139215 1106 20-21 0.41233354237785846 1106 22-23 0.41231771903225223 1106 24-25 0.47156419452385734 1106 26-27 0.3916531979805242 1106 28-29 0.425912511594575 1106 30-31 0.41603756812493486 1106 32-33 0.4830680340296283 1106 34-35 0.3639829911782897 1106 36-37 0.569247378944155 1106 38-39 0.40530944000874314 1106 40-41 0.589695918066262 1106 42-43 0.5728641248555704 1106 44-45 0.46042546911335336 1106 46-47 0.36998812371363243 1106 48-49 0.5881260553159962 1106 50-51 0.6526751001888798 1106 52-53 0.5957308697603025 1106 54-55 0.5615912375311254 1106 56-57 0.5998758313771404 1106 58-59 0.6296929079248841 1106 60-61 0.7930317372785396 1106 62-63 0.7338687980728835 1106 64-65 0.6176980346254126 1106 66-67 0.5624694084202915 1106 68-69 0.6502407641526702 1106 70-71 0.5716020013621659 1106 72-73 0.5948101804839823 1106 74-75 0.5935378429035225 1106 76-77 0.6205764124804034 1106 78-79 0.46196294772180835 1106 80-81 0.4108068097796398 1106 82-83 0.4722886497788643 1106 84-85 0.5380502724326242 1106 86-87 0.5018035703438635 1106 88-89 0.5251726180310072 1106 90-91 0.5356731245510318 1106 92-93 0.502031597179176 1106 94-95 0.6065972413411274 1106 96-97 0.6188690775063037 1106 98-99 0.49469003301244996 1106 100-101 0.38364803691506566 1107 1 0.13997606017591835 1107 2 0.24840299442024616 1107 3 0.2079996616865003 1107 4 0.24832497128676323 1107 5 0.35956729872229687 1107 6 0.2978700328090156 1107 7 0.31469448223562324 1107 8 0.2822579214308121 1107 9 0.24190913915974477 1107 10-11 0.2576104998884716 1107 12-13 0.4099836724804433 1107 14-15 0.46421839089870787 1107 16-17 0.48106592920193236 1107 18-19 0.46776184576611257 1107 20-21 0.5298388220034695 1107 22-23 0.4752381100599763 1107 24-25 0.4287128210161697 1107 26-27 0.5071904585860736 1107 28-29 0.40941874651711885 1107 30-31 0.512654180094593 1107 32-33 0.5438946878874447 1107 34-35 0.5438126746154239 1107 36-37 0.5606450105591136 1107 38-39 0.5393769118798062 1107 40-41 0.491719886358986 1107 42-43 0.4524353951053186 1107 44-45 0.5050335424458083 1107 46-47 0.6411887387531152 1107 48-49 0.64916939520581 1107 50-51 0.5228408549405401 1107 52-53 0.59002592234188 1107 54-55 0.5991890145873597 1107 56-57 0.4910512759524792 1107 58-59 0.44215188516681536 1107 60-61 0.3930734644339253 1107 62-63 0.43398431923298375 1107 64-65 0.4493862536138167 1107 66-67 0.48116505242727925 1107 68-69 0.5273355076044624 1107 70-71 0.48383570563336065 1107 72-73 0.5390939627084848 1107 74-75 0.4819155555040524 1107 76-77 0.5246798958257628 1107 78-79 0.6360965390210929 1107 80-81 0.6060559517566197 1107 82-83 0.6512664820598495 1107 84-85 0.7256662967336602 1107 86-87 0.6710993608654938 1107 88-89 0.6911184290807846 1107 90-91 0.7032662861277004 1107 92-93 0.6714121837207578 1107 94-95 0.8545963504462328 1107 96-97 0.7605974404332692 1107 98-99 0.7755243882809388 1107 100-101 0.6206292986047046 1108 1 -0.04687621379102325 1108 2 0.009688009794729169 1108 3 -0.02487897047270593 1108 4 0.061774915097224437 1108 5 0.01748130706434381 1108 6 0.07591560766741878 1108 7 -0.08591449441531296 1108 8 0.03751998182530514 1108 9 0.043102560103179144 1108 10-11 0.08639577755040051 1108 12-13 0.1456639521702101 1108 14-15 0.09196307899065204 1108 16-17 0.05714625336312196 1108 18-19 0.05453853919045315 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-1.2699664562502377 2316 76-77 -1.3608297867079742 2316 78-79 -1.0296872936644625 2316 80-81 -1.0061165792271147 2316 82-83 -1.126232395318599 2316 84-85 -0.9320473172598618 2316 86-87 -1.0686141946817393 2316 88-89 -1.2984211252703552 2316 90-91 -1.3397215788580752 2316 92-93 -1.2089931207329734 2316 94-95 -1.1050845574963297 2316 96-97 -1.238778480764669 2316 98-99 -1.7000064748596593 2316 100-101 -1.64122902182174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7965.0 3 2354.0 4 2340.0 5 2491.0 6 2831.0 7 3337.0 8 3828.0 9 4631.0 10 5717.0 11 6761.0 12 8584.0 13 12534.0 14 10610.0 15 10367.0 16 12013.0 17 16454.0 18 14103.0 19 10901.0 20 10627.0 21 11701.0 22 12442.0 23 14119.0 24 15657.0 25 17668.0 26 20006.0 27 23260.0 28 27329.0 29 32166.0 30 38583.0 31 47101.0 32 59150.0 33 77763.0 34 108166.0 35 159412.0 36 261935.0 37 432976.0 38 431389.0 39 75812.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.101484428251965 18.915797361990823 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27.04867527736586 28.633261153928398 21.815300030472805 20-21 23.096011461742165 26.30096311714088 28.654355409869286 21.948670011247668 22-23 22.837920270546835 26.15180109561931 28.556937829465685 22.45334080436817 24-25 22.815249762214616 26.247482108031694 28.567432804670208 22.369835325083482 26-27 22.40308203873812 26.61045541250885 28.485383840162836 22.501078708590196 28-29 22.312468904306364 26.74720228799723 28.62773570691022 22.312593100786188 30-31 22.49756165892135 26.44515328329736 28.47550221630116 22.581782841480134 32-33 22.283766690335703 26.51473776418733 28.488656122643867 22.7128394228331 34-35 22.88321724933588 26.612512670556082 28.16856028869073 22.33570979141731 36-37 22.78359555971913 26.274422729807238 28.2575552509103 22.68442645956333 38-39 22.495873120080855 26.56543318395583 28.449605171458202 22.48908852450511 40-41 22.790781877652435 26.442666270347022 28.28682437495496 22.479727477045586 42-43 22.566450705925725 26.486844054793863 28.085721797268288 22.860983442012124 44-45 22.67181380497418 26.71963479432235 27.95000873101005 22.658542669693414 46-47 22.74768128345928 26.825718776558816 27.613662074176474 22.812937865805427 48-49 22.804665086690783 26.879231632780343 27.587052513409006 22.729050767119872 50-51 22.682247366815275 27.04125607545274 27.49481171366497 22.78168484406702 52-53 22.960093957615296 26.996050507207475 27.35377995797053 22.6900755772067 54-55 22.626178267494033 26.866619171772864 27.531971746524942 22.97523081420816 56-57 22.63229242362503 27.207084855978202 27.453268289568776 22.70735443082799 58-59 22.729710704884273 26.79912717001259 27.564760947964583 22.90640117713856 60-61 22.820378622484075 26.730842081127694 27.786035429813282 22.662743866574953 62-63 22.640993671705342 26.88336788085557 27.765383457543958 22.71025498989513 64-65 22.529712900038916 26.78833737488649 27.634568751309764 23.04738097376483 66-67 22.44411576255861 27.028853811745122 27.456980265114545 23.070050160581726 68-69 22.52877481331682 26.819194055684044 27.676025197862714 22.976005933136427 70-71 22.446877928092597 26.820236480711863 27.624824860293725 23.10806073090182 72-73 22.637355563744563 26.705310244323783 27.560307508601994 23.09702668332966 74-75 22.44281627729131 26.982134102145377 27.36507635516222 23.20997326540109 76-77 22.521295747286224 26.830134657919793 27.475424050344 23.173145544449987 78-79 22.51019334309234 26.87364244620284 27.45297346364187 23.163190747062945 80-81 22.339884818623748 27.032458482929794 27.452018567086217 23.17563813136024 82-83 22.525888076705783 26.899978424004477 27.40301091146733 23.171122587822406 84-85 22.495386410419044 26.792191554194183 27.372509914946335 23.339912120440438 86-87 22.468034840109972 26.741787255439682 27.5435008768392 23.246677027611142 88-89 22.400601665806075 26.658210418397204 27.45696418192658 23.484223733870145 90-91 22.374784095453823 26.70806911041214 27.585783619671684 23.33136317446235 92-93 22.497789982601805 26.503267182932056 27.57193503709062 23.427007797375524 94-95 22.361368229335596 26.38319902198049 27.626132082532873 23.629300666151043 96-97 22.465216832645343 26.26003450560061 27.6194254306027 23.655323231151346 98-99 22.218127654053625 26.251914910604242 27.511364713748932 24.018592721593205 100-101 22.484080198533253 25.61543386993418 28.072255407269793 23.82823052426278 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1725.0 1 961.0 2 218.0 3 252.0 4 251.5 5 249.5 6 391.5 7 799.0 8 1342.0 9 1653.0 10 1678.0 11 1722.0 12 1904.5 13 2131.5 14 2345.5 15 2529.5 16 2686.0 17 2820.5 18 2930.5 19 3007.5 20 3193.0 21 3527.0 22 4076.0 23 4800.5 24 5814.0 25 7397.0 26 9648.5 27 12447.0 28 14832.5 29 17522.5 30 20853.0 31 24372.0 32 28552.5 33 32576.5 34 35941.5 35 39395.5 36 44046.5 37 48660.5 38 51780.5 39 53825.0 40 55947.5 41 60028.5 42 64148.0 43 67897.0 44 72228.5 45 74736.5 46 75353.5 47 77948.0 48 83484.5 49 84299.5 50 81368.5 51 78849.0 52 75267.5 53 73642.5 54 71841.5 55 65912.5 56 58782.0 57 52767.0 58 47067.5 59 41498.5 60 35128.0 61 28498.5 62 22904.5 63 17392.0 64 13026.5 65 9986.5 66 7459.5 67 5899.5 68 4527.5 69 3305.0 70 2489.0 71 1865.5 72 1475.0 73 1187.5 74 871.0 75 658.5 76 488.5 77 277.0 78 154.5 79 106.0 80 82.0 81 61.0 82 43.5 83 37.5 84 28.0 85 19.0 86 10.5 87 9.0 88 9.0 89 7.0 90 8.0 91 6.0 92 5.0 93 5.5 94 2.5 95 1.0 96 1.0 97 2.0 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4038002982474859 2 0.11900124311140114 3 0.1202418732522623 4 0.1325985494552393 5 0.13468280809188604 6 0.07746494599536996 7 0.08719148629972136 8 0.08391622272784793 9 0.08337034546586902 10-11 0.08180715148838398 12-13 0.08575235533632243 14-15 0.07940032901511335 16-17 0.08098833559541561 18-19 0.0910374397363909 20-21 0.08925093233355086 22-23 0.09910153565198836 24-25 0.11217777733666483 26-27 0.11321990665498821 28-29 0.10746338280139248 30-31 0.09694283920688997 32-33 0.09560295865475993 34-35 0.34759975286647593 36-37 0.5435200647112681 38-39 0.5242406723222859 40-41 0.4957309916852968 42-43 0.5247369243786304 44-45 0.5337190865984651 46-47 0.5314611397420979 48-49 0.5386816071619097 50-51 0.5374657896238657 52-53 0.5358033452351119 54-55 0.5299475709702471 56-57 0.5010160760853654 58-59 0.49146322400073444 60-61 0.44091995206205137 62-63 0.5150351966770962 64-65 0.5421553715563209 66-67 0.5528744159733612 68-69 0.5678612280749639 70-71 0.5631220209368742 72-73 0.5490284625366916 74-75 0.5531969798099852 76-77 0.5287813786378377 78-79 0.502207081020592 80-81 0.5095019862488556 82-83 0.5241662345138343 84-85 0.517566082164453 86-87 0.5191540887447552 88-89 0.5295009441195372 90-91 0.5476885819845616 92-93 0.5538917326888673 94-95 0.5486066482887989 96-97 0.549971341443746 98-99 0.5505916565141767 100-101 0.526250493150481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2015105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.105895151998446 #Duplication Level Percentage of deduplicated Percentage of total 1 79.29795725199772 47.6627470435623 2 12.102209446920174 14.548282642482183 3 3.752733548827289 6.7668422765760035 4 1.606445971929097 3.862274926244822 5 0.8510627082572411 2.557694295514279 6 0.5291925767597963 1.9084556120364125 7 0.3457372378652339 1.4546592318488507 8 0.23890814196436866 1.1487830185495282 9 0.18212705010439056 0.985221844012554 >10 0.985668210651537 10.727761124195315 >50 0.06705900730773656 2.7883820344169195 >100 0.03833360890900421 4.120249844244718 >500 0.0021514903601964164 0.7836128218982475 >1k 3.309985169532919E-4 0.4332625383405638 >5k 8.274962923832298E-5 0.2517707460773658 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5062 0.2512027909215649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3985 0.1977564444532667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2419 0.12004337242972452 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.9625205634445846E-5 6 0.0 0.0 0.0 0.0 4.9625205634445846E-5 7 0.0 0.0 0.0 0.0 4.9625205634445846E-5 8 0.0 0.0 0.0 0.0 4.9625205634445846E-5 9 0.0 0.0 0.0 0.0 4.9625205634445846E-5 10-11 0.0 0.0 0.0 0.0 4.9625205634445846E-5 12-13 0.0 0.0 0.0 4.9625205634445846E-5 9.925041126889169E-5 14-15 0.0 0.0 0.0 4.9625205634445846E-5 9.925041126889169E-5 16-17 0.0 0.0 0.0 7.443780845166876E-5 9.925041126889169E-5 18-19 0.0 0.0 0.0 9.925041126889169E-5 1.2406301408611462E-4 20-21 0.0 0.0 0.0 1.2406301408611462E-4 1.4887561690333755E-4 22-23 0.0 0.0 0.0 1.9850082253778338E-4 1.4887561690333755E-4 24-25 0.0 0.0 0.0 3.7218904225834383E-4 1.4887561690333755E-4 26-27 0.0 0.0 0.0 7.691906873339106E-4 1.4887561690333755E-4 28-29 0.0 0.0 0.0 0.002878261926797859 1.7368821972056045E-4 30-31 0.0 0.0 0.0 0.008634785780393577 1.9850082253778338E-4 32-33 0.0 0.0 0.0 0.01893201594954109 1.9850082253778338E-4 34-35 0.0 0.0 0.0 0.03493614476664988 1.9850082253778338E-4 36-37 0.0 0.0 0.0 0.06054275087402393 2.233134253550063E-4 38-39 0.0 0.0 0.0 0.09815865674493388 2.4812602817222924E-4 40-41 0.0 0.0 0.0 0.1432183434610107 2.4812602817222924E-4 42-43 0.0 0.0 0.0 0.18698777483059198 2.4812602817222924E-4 44-45 0.0 0.0 0.0 0.24576883090459306 2.7293863098945217E-4 46-47 0.0 0.0 0.0 0.3157651834519789 3.4737643944112096E-4 48-49 0.0 0.0 0.0 0.3955873267149851 3.4737643944112096E-4 50-51 0.0 0.0 0.0 0.48089305520059744 3.4737643944112096E-4 52-53 0.0 0.0 0.0 0.5631716461425087 3.4737643944112096E-4 54-55 0.0 0.0 0.0 0.6630671850846481 3.4737643944112096E-4 56-57 0.0 0.0 0.0 0.7789420402410792 3.4737643944112096E-4 58-59 0.0 0.0 0.0 0.9131534088794381 3.4737643944112096E-4 60-61 0.0 0.0 0.0 1.0638651583912502 3.4737643944112096E-4 62-63 0.0 0.0 0.0 1.2184972991481833 3.4737643944112096E-4 64-65 0.0 0.0 0.0 1.376975393341786 3.4737643944112096E-4 66-67 4.9625205634445846E-5 0.0 0.0 1.5653526739301427 3.4737643944112096E-4 68-69 4.9625205634445846E-5 0.0 0.0 1.7878720959949979 3.4737643944112096E-4 70-71 4.9625205634445846E-5 0.0 0.0 2.0251302041332835 3.4737643944112096E-4 72-73 4.9625205634445846E-5 0.0 0.0 2.2741742986097497 3.4737643944112096E-4 74-75 4.9625205634445846E-5 0.0 0.0 2.5298681706412323 3.4737643944112096E-4 76-77 4.9625205634445846E-5 0.0 0.0 2.8163296701660707 3.4737643944112096E-4 78-79 4.9625205634445846E-5 0.0 0.0 3.1412258914547877 3.4737643944112096E-4 80-81 4.9625205634445846E-5 0.0 0.0 3.488627143498726 3.4737643944112096E-4 82-83 4.9625205634445846E-5 0.0 0.0 3.858955240545778 3.4737643944112096E-4 84-85 4.9625205634445846E-5 0.0 0.0 4.2419873902352485 3.4737643944112096E-4 86-87 4.9625205634445846E-5 0.0 0.0 4.642686113130582 3.9700164507556676E-4 88-89 4.9625205634445846E-5 0.0 0.0 5.089040025209604 4.466268507100126E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 34.188118 1 GTATCAA 7265 0.0 33.337063 1 ATCAACG 8735 0.0 27.161718 3 TCAACGC 8865 0.0 26.816826 4 TATCAAC 9070 0.0 26.36735 2 CAACGCA 9000 0.0 26.361954 5 AACGCAG 9330 0.0 25.516468 6 ACGCAGA 10455 0.0 22.589714 7 CGCAGAG 10585 0.0 22.08816 8 GTACATG 7370 0.0 21.134848 1 TACCTGG 1750 0.0 21.107603 2 TACATGG 7340 0.0 20.51698 2 GTACCTG 1880 0.0 20.460674 1 ACATGGG 7445 0.0 20.036795 3 GAGTACT 5470 0.0 19.77145 12-13 GCAGAGT 11530 0.0 19.579992 9 AGTACTT 5940 0.0 19.163212 12-13 GTATAGG 455 0.0 18.786846 1 AGAGTAC 9545 0.0 18.768032 10-11 GTACTTT 5900 0.0 18.37173 14-15 >>END_MODULE