FastQCFastQC Report
Fri 27 May 2016
SRR939138_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939138_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015105
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.24415601172147358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.21120487518020153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.130117289173517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.038.3046951
GTATCAA71200.034.9450071
TATAGCG851.6448099E-533.493565
ATCAACG86850.027.9723593
TCAACGC89050.027.1747284
TATCAAC90300.027.0612872
CAACGCA89900.026.9177935
AACGCAG91800.026.4640466
ACGCAGA104700.023.1127957
CGCAGAG104600.023.0895298
GTACATG70750.022.2995551
GAGTACT55800.021.344712-13
TACATGG72500.021.0085472
GCAGAGT115850.020.7654299
ACATGGG72300.020.016613
AGAGTAC97000.019.8856310-11
AGTACTT61250.019.13556912-13
ATAACGC1507.7795895E-418.9796813
GTACTTT62500.018.82878114-15
ATAGCGT1559.697376E-418.3674356
Produced by FastQC (version 0.11.5)