##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939138_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2015105 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53444063708839 34.0 31.0 34.0 31.0 34.0 2 32.754318013205264 34.0 31.0 34.0 31.0 34.0 3 32.862758516305604 34.0 31.0 34.0 31.0 34.0 4 36.30114857538441 37.0 37.0 37.0 35.0 37.0 5 36.258718032062845 37.0 37.0 37.0 35.0 37.0 6 36.294005523285385 37.0 37.0 37.0 35.0 37.0 7 36.24883169859635 37.0 37.0 37.0 35.0 37.0 8 36.223165542242214 37.0 37.0 37.0 35.0 37.0 9 37.98033303475501 39.0 38.0 39.0 35.0 39.0 10-11 37.89745273819479 39.0 38.0 39.0 35.0 39.0 12-13 37.81661650385463 39.0 38.0 39.0 35.0 39.0 14-15 39.25470136791879 40.5 39.0 41.0 36.0 41.0 16-17 39.34418603497088 41.0 39.0 41.0 36.0 41.0 18-19 39.36280938214138 41.0 39.0 41.0 36.0 41.0 20-21 39.32597929140169 41.0 39.0 41.0 36.0 41.0 22-23 39.26199230313061 41.0 39.0 41.0 36.0 41.0 24-25 39.194401284300326 41.0 39.0 41.0 36.0 41.0 26-27 39.058817282474116 40.5 39.0 41.0 35.0 41.0 28-29 38.91572449078336 40.0 38.0 41.0 35.0 41.0 30-31 38.74049615280593 40.0 38.0 41.0 35.0 41.0 32-33 38.67648187067175 40.0 38.0 41.0 35.0 41.0 34-35 38.58784827589629 40.0 38.0 41.0 35.0 41.0 36-37 38.455483709285616 40.0 38.0 41.0 34.0 41.0 38-39 38.33526764114029 40.0 38.0 41.0 34.0 41.0 40-41 38.165898054940065 40.0 38.0 41.0 34.0 41.0 42-43 37.99426059684235 40.0 38.0 41.0 33.0 41.0 44-45 37.76198560372785 40.0 37.5 41.0 33.0 41.0 46-47 37.47646375747169 40.0 37.0 41.0 33.0 41.0 48-49 37.16392718989829 40.0 37.0 41.0 31.5 41.0 50-51 36.91375412199364 40.0 36.5 41.0 31.0 41.0 52-53 36.64637599529553 39.0 36.0 41.0 31.0 41.0 54-55 36.399893553933914 39.0 36.0 41.0 30.0 41.0 56-57 36.15861183412279 39.0 35.0 40.0 30.0 41.0 58-59 35.817453929199715 38.5 35.0 40.0 29.0 41.0 60-61 35.44135715012369 38.0 35.0 40.0 28.0 41.0 62-63 35.03529071686091 38.0 34.0 40.0 28.0 41.0 64-65 34.575209728525316 37.0 34.0 40.0 27.0 41.0 66-67 34.14999789092876 36.5 34.0 39.0 26.0 41.0 68-69 33.65835849744802 36.0 33.0 39.0 26.0 40.0 70-71 33.14242309954072 35.0 33.0 38.5 25.0 40.0 72-73 32.56151714178665 35.0 32.0 37.5 23.5 39.5 74-75 32.000780108232576 35.0 31.5 37.0 22.0 39.0 76-77 30.00800653067706 33.0 29.0 35.0 20.0 37.0 78-79 30.990930497418248 34.0 30.5 36.0 20.0 38.0 80-81 30.999845169358423 34.0 31.0 35.5 20.0 37.0 82-83 30.610568183791912 34.0 31.0 35.0 18.0 37.0 84-85 30.141367323290844 34.0 30.5 35.0 14.5 36.5 86-87 29.43947883609043 34.0 29.5 35.0 7.0 36.0 88-89 28.768119775396322 34.0 29.0 35.0 2.0 35.5 90-91 28.20985258832666 33.0 28.0 35.0 2.0 35.0 92-93 27.750472804146682 33.0 27.0 35.0 2.0 35.0 94-95 27.21142893298364 33.0 26.5 34.5 2.0 35.0 96-97 26.43201173139861 32.0 25.0 34.0 2.0 35.0 98-99 25.229368692946522 32.0 21.0 34.0 2.0 35.0 100-101 19.405968423481653 25.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19939521978004393 1101 2 0.15581598496667937 1101 3 0.12781706188183506 1101 4 0.11743076544626518 1101 5 0.1486195635663634 1101 6 0.13622095483474794 1101 7 0.21055519722525418 1101 8 0.15173093176644414 1101 9 0.213498386089924 1101 10-11 0.27202717217721784 1101 12-13 0.26151420544523774 1101 14-15 0.3392925872396759 1101 16-17 0.23380375479209903 1101 18-19 0.1824277135917427 1101 20-21 0.23825964516307607 1101 22-23 0.324762454391454 1101 24-25 0.37932468717698953 1101 26-27 0.2999454988549175 1101 28-29 0.3020647334247357 1101 30-31 0.29311998830402786 1101 32-33 0.38321378711849263 1101 34-35 0.44730944321248245 1101 36-37 0.4475230913517194 1101 38-39 0.37698043767931466 1101 40-41 0.5328131816235739 1101 42-43 0.46935616135416325 1101 44-45 0.5959548242028276 1101 46-47 0.5111144062793187 1101 48-49 0.5203049661014774 1101 50-51 0.3476920420950691 1101 52-53 0.37652838905411556 1101 54-55 0.3860954356465953 1101 56-57 0.4655146348076471 1101 58-59 0.34475828961784316 1101 60-61 0.45812149231463906 1101 62-63 0.4758416226125348 1101 64-65 0.25730392843247074 1101 66-67 0.3334521039906875 1101 68-69 0.41875941805820105 1101 70-71 0.37760707729044185 1101 72-73 0.5126691402705106 1101 74-75 0.18886746610913718 1101 76-77 0.35956272982067006 1101 78-79 0.38139862871170394 1101 80-81 0.5276202148965581 1101 82-83 0.39861359931287055 1101 84-85 0.3735728862778167 1101 86-87 0.7424463845573683 1101 88-89 0.6250621584882197 1101 90-91 0.9030181179168473 1101 92-93 0.7843456533494546 1101 94-95 0.7208798296250798 1101 96-97 0.44793658948696446 1101 98-99 0.42492231337314124 1101 100-101 -0.5550043303664154 1102 1 0.1273825020077055 1102 2 0.06272301190728058 1102 3 0.09756766034858089 1102 4 0.06770373426304843 1102 5 0.10061381274632453 1102 6 0.1524583977341294 1102 7 0.17739796806804264 1102 8 0.10835208579538147 1102 9 0.0966052263303041 1102 10-11 0.19496454703110544 1102 12-13 0.1927703152194269 1102 14-15 0.24386302880521527 1102 16-17 0.2688339661108117 1102 18-19 0.2294006647204725 1102 20-21 0.20370216434454846 1102 22-23 0.14881889753192468 1102 24-25 0.17403910696796743 1102 26-27 0.12290792653690374 1102 28-29 0.15648057763894485 1102 30-31 0.022257371592786512 1102 32-33 0.04222874516759845 1102 34-35 0.15543219325764568 1102 36-37 0.21254959510365978 1102 38-39 0.09764124693601417 1102 40-41 0.20925449033799026 1102 42-43 0.24104621380773494 1102 44-45 0.21903061907934074 1102 46-47 0.05019555918780583 1102 48-49 0.17838012836260475 1102 50-51 0.18218632432209603 1102 52-53 0.1626837744994276 1102 54-55 0.2987704356308569 1102 56-57 0.3485272122086087 1102 58-59 0.10105265531233698 1102 60-61 -0.04216617458094163 1102 62-63 -0.056605723959350485 1102 64-65 -0.11310276457533774 1102 66-67 0.1788786724519511 1102 68-69 0.3034344205603361 1102 70-71 0.09801971877125482 1102 72-73 0.11391529815494295 1102 74-75 -0.08818943087779019 1102 76-77 -0.04395083490092233 1102 78-79 0.12244318686605027 1102 80-81 0.15874029662668931 1102 82-83 0.1322050984728449 1102 84-85 0.1135940867614984 1102 86-87 0.2658506358586372 1102 88-89 0.027377688157251612 1102 90-91 0.2471103714942693 1102 92-93 0.17948508996296653 1102 94-95 0.21222023775957055 1102 96-97 0.44621310598563824 1102 98-99 0.37225818430197677 1102 100-101 -0.14406681954743306 1103 1 0.10299175513774372 1103 2 0.10496649944382597 1103 3 0.10264652729414081 1103 4 0.08416513721763152 1103 5 0.04897184365196239 1103 6 0.09839057056745304 1103 7 0.06028063983638532 1103 8 0.10869265577204601 1103 9 0.09751445443313855 1103 10-11 0.08555519222998953 1103 12-13 0.120128926343817 1103 14-15 -0.022790921165999123 1103 16-17 0.20264715941676315 1103 18-19 0.241440594519311 1103 20-21 0.23820971421852022 1103 22-23 0.26847206606687735 1103 24-25 0.23849775536758244 1103 26-27 0.14312684741749848 1103 28-29 0.3394078984436817 1103 30-31 0.25444454635236013 1103 32-33 0.18851042403385065 1103 34-35 0.27179604947212255 1103 36-37 0.19870683157514435 1103 38-39 0.24847812735252717 1103 40-41 0.3618601474865031 1103 42-43 0.20219514693322793 1103 44-45 0.23155094001499066 1103 46-47 0.27340699658994794 1103 48-49 0.05580618171585883 1103 50-51 0.20978873980684654 1103 52-53 0.30398516424364885 1103 54-55 0.13740463129362013 1103 56-57 -0.08403774526544794 1103 58-59 0.05029054406593758 1103 60-61 0.3568941959779366 1103 62-63 0.3436817920888302 1103 64-65 0.2338894557440696 1103 66-67 0.2601289829486646 1103 68-69 0.19035722084541362 1103 70-71 0.1959212453058754 1103 72-73 0.1142603986496411 1103 74-75 0.10956299474594289 1103 76-77 0.10569936223910403 1103 78-79 -0.03452629650179162 1103 80-81 0.021481534995363205 1103 82-83 0.012892656139310787 1103 84-85 -0.11768385989524077 1103 86-87 -0.11972222627582241 1103 88-89 0.24322656507307627 1103 90-91 0.340582926084668 1103 92-93 0.17419516336673269 1103 94-95 0.15976121317072867 1103 96-97 0.06552836036485132 1103 98-99 -0.4416600045406369 1103 100-101 -1.0467848804107263 1104 1 0.15186795023296895 1104 2 0.14198986470623964 1104 3 0.16827912878835605 1104 4 0.1526400707174176 1104 5 0.10272107835690747 1104 6 0.08715446750223066 1104 7 0.14508476172674278 1104 8 0.1662042231412144 1104 9 0.2224788654066856 1104 10-11 0.199888401319825 1104 12-13 0.2375616365136679 1104 14-15 0.15190277370095373 1104 16-17 0.19857062542120474 1104 18-19 0.2358288300071507 1104 20-21 0.2257712933384255 1104 22-23 0.24953047098991732 1104 24-25 0.19826756060466977 1104 26-27 0.2674847106089473 1104 28-29 0.22572734195255606 1104 30-31 0.26114261818306517 1104 32-33 0.18046707951860697 1104 34-35 0.12229519398827193 1104 36-37 0.15734766756923335 1104 38-39 0.29812523090723886 1104 40-41 0.10827662067760713 1104 42-43 0.14635233441966022 1104 44-45 0.26997750836462586 1104 46-47 0.220194403987378 1104 48-49 0.20340221969544814 1104 50-51 0.03661021686745869 1104 52-53 0.0675794238945997 1104 54-55 -0.0050472767422391485 1104 56-57 0.0215219267915856 1104 58-59 0.016955414441049754 1104 60-61 0.1917394080376411 1104 62-63 0.1221295866170351 1104 64-65 0.024580773518529497 1104 66-67 0.06232450030308456 1104 68-69 0.001335406381372195 1104 70-71 -0.2213304884897056 1104 72-73 -0.3130002716964242 1104 74-75 -0.30942021441873635 1104 76-77 -0.25925469011979985 1104 78-79 0.021729663069670835 1104 80-81 0.16319411936493466 1104 82-83 -0.019364548860966835 1104 84-85 -0.14472583174435982 1104 86-87 -0.10690508725932446 1104 88-89 0.012854418369943943 1104 90-91 0.215063311329331 1104 92-93 0.08886951816126043 1104 94-95 -0.035289255137449516 1104 96-97 -0.5485452845951322 1104 98-99 -0.5125599316411389 1104 100-101 -0.7254217346252005 1105 1 -0.01650434532209033 1105 2 0.0011299067137215957 1105 3 0.05209742651778271 1105 4 -0.028263602375595553 1105 5 -0.05817126751605173 1105 6 -0.06808849852179577 1105 7 0.018369029413427995 1105 8 -0.009858344096372207 1105 9 0.11836179090317245 1105 10-11 0.09121468217796291 1105 12-13 0.048692480135422045 1105 14-15 0.07540618609714755 1105 16-17 0.12281016481886553 1105 18-19 0.12930258671668327 1105 20-21 0.06847035926967493 1105 22-23 0.037219376575549745 1105 24-25 0.044953068024291554 1105 26-27 0.14036711812702407 1105 28-29 0.14339865904072013 1105 30-31 0.11861901032118283 1105 32-33 0.15290778237545766 1105 34-35 0.25522567104069793 1105 36-37 0.27374097255606245 1105 38-39 0.18740222412798602 1105 40-41 0.2695945911129911 1105 42-43 0.19288017148983982 1105 44-45 0.21884720548956693 1105 46-47 0.2486899044057438 1105 48-49 0.3282734366031761 1105 50-51 0.13617847332938027 1105 52-53 0.29848082015946886 1105 54-55 0.29749693641441155 1105 56-57 0.2142766327107708 1105 58-59 0.3561283850756567 1105 60-61 0.2188557198037202 1105 62-63 0.12329731485482398 1105 64-65 0.13562566687758704 1105 66-67 0.0733018003264263 1105 68-69 -0.0060218791841037955 1105 70-71 -0.013758611871026005 1105 72-73 -0.14212858803892914 1105 74-75 -0.04109075169326104 1105 76-77 -0.17661009730205635 1105 78-79 -0.1932863031877332 1105 80-81 0.08011066792114008 1105 82-83 -0.08134223025301779 1105 84-85 -0.04514049907130513 1105 86-87 0.03855278288178354 1105 88-89 -6.507301077114391E-4 1105 90-91 0.11085857894201823 1105 92-93 -0.09194578553915278 1105 94-95 -0.1223802087300001 1105 96-97 -0.058460301984403884 1105 98-99 -0.2881140546923149 1105 100-101 -0.7410679553750725 1106 1 0.0465039199653674 1106 2 0.027244556395245922 1106 3 0.10178699318611706 1106 4 0.06949795156496918 1106 5 0.10703442228344073 1106 6 0.09202220422165652 1106 7 0.09999414790472372 1106 8 0.05431487770270138 1106 9 0.07368599971393763 1106 10-11 0.09706953796504081 1106 12-13 0.1422620754079702 1106 14-15 0.09087546705593752 1106 16-17 0.03134139940113556 1106 18-19 0.04795547236080466 1106 20-21 0.12129835148667212 1106 22-23 0.13292458686640884 1106 24-25 0.16111694356269624 1106 26-27 0.15203941139782984 1106 28-29 0.20814147056888288 1106 30-31 0.1763625203243393 1106 32-33 0.10238309461040984 1106 34-35 0.11133053652881841 1106 36-37 0.17946528004026874 1106 38-39 0.2868126779704241 1106 40-41 0.3098474002574747 1106 42-43 0.23105543327937283 1106 44-45 0.1827719420369931 1106 46-47 0.16752918863517863 1106 48-49 0.05098690109459625 1106 50-51 0.003831042996715439 1106 52-53 -0.005846121573313212 1106 54-55 0.0349600031631212 1106 56-57 0.03982598883878552 1106 58-59 -0.022852368057776573 1106 60-61 0.05650116613478673 1106 62-63 -0.0013691078999400474 1106 64-65 0.10821218631016194 1106 66-67 0.07397934879005419 1106 68-69 -0.006320394093222603 1106 70-71 -0.03700258475245022 1106 72-73 -0.034242195551414056 1106 74-75 -0.06830064897308574 1106 76-77 -0.05034660592489715 1106 78-79 -0.030421058970937054 1106 80-81 0.12658669526144806 1106 82-83 -0.07172946811701664 1106 84-85 -0.12870444002245307 1106 86-87 -0.09234877649136308 1106 88-89 -0.13438166756094105 1106 90-91 0.0897208407814496 1106 92-93 -0.0467166192189552 1106 94-95 0.09105490238850322 1106 96-97 -0.291543625616697 1106 98-99 -0.24590329817737455 1106 100-101 -0.5874493282664801 1107 1 0.06485313466298237 1107 2 0.07256829740244086 1107 3 0.08565911220108546 1107 4 0.09168847963486826 1107 5 0.06707939637503557 1107 6 0.08002249403083539 1107 7 0.11067334792404182 1107 8 0.15344249839615287 1107 9 0.1434860045440871 1107 10-11 0.1656874970107154 1107 12-13 0.19970560957727201 1107 14-15 0.19577995077965937 1107 16-17 0.2010088433646544 1107 18-19 0.16010451478295806 1107 20-21 0.19582452689099483 1107 22-23 0.2001438140955969 1107 24-25 0.1975263688013129 1107 26-27 0.2357961434236273 1107 28-29 0.17035907432995856 1107 30-31 0.18833002949492084 1107 32-33 0.11256371549974631 1107 34-35 0.019810272695131914 1107 36-37 0.09718603815962723 1107 38-39 0.10196647809332404 1107 40-41 0.08802637889477438 1107 42-43 0.08892911941066473 1107 44-45 0.17148427690613488 1107 46-47 0.07739209279151993 1107 48-49 0.062339504949591173 1107 50-51 -0.13997294402627602 1107 52-53 -0.11060046804018242 1107 54-55 -0.03370370816546853 1107 56-57 -0.03888162321405986 1107 58-59 -0.018741236970107877 1107 60-61 -0.21758720956524513 1107 62-63 -0.20942231471904194 1107 64-65 -0.18862594184453485 1107 66-67 -0.3128261489974662 1107 68-69 -0.4644716798531192 1107 70-71 -0.44671052149891466 1107 72-73 -0.45651456788107936 1107 74-75 -0.5920742597003184 1107 76-77 -0.5519496800489598 1107 78-79 -0.5030119530591008 1107 80-81 -0.38879828135256034 1107 82-83 -0.5709019018969599 1107 84-85 -0.4706543191344572 1107 86-87 -0.4744754660386441 1107 88-89 -0.5549618762772184 1107 90-91 -0.0026858903051483196 1107 92-93 0.029188699047878686 1107 94-95 -0.04814769301372834 1107 96-97 -0.1885780458239097 1107 98-99 -0.3725605102667089 1107 100-101 -1.425107348064742 1108 1 -0.12051701947851967 1108 2 0.013938309569446972 1108 3 -0.027176165539138708 1108 4 -0.03948369211043001 1108 5 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2316 82-83 0.08435820262311822 2316 84-85 0.0331625941522411 2316 86-87 0.0656797088660035 2316 88-89 0.04756094555913748 2316 90-91 -0.2895701308388041 2316 92-93 -0.27222932323662263 2316 94-95 0.02084856601200613 2316 96-97 0.10214728912067628 2316 98-99 0.2590715489237425 2316 100-101 1.3797303996785928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 12.0 5 35.0 6 58.0 7 143.0 8 376.0 9 784.0 10 1335.0 11 2071.0 12 3212.0 13 4568.0 14 5868.0 15 7905.0 16 10105.0 17 14442.0 18 14908.0 19 10814.0 20 10554.0 21 11195.0 22 11947.0 23 13230.0 24 15195.0 25 17719.0 26 20610.0 27 24946.0 28 30167.0 29 36768.0 30 44945.0 31 55198.0 32 69348.0 33 90521.0 34 126091.0 35 189477.0 36 313918.0 37 501079.0 38 338997.0 39 16558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.413720916322745 19.344159339673425 14.94327810674482 23.298841637259006 2 15.542068527446459 20.187087025241862 40.308916905074426 23.96192754223725 3 19.426431873277075 24.109661779411 29.56312450219716 26.900781845114768 4 13.47751109743661 16.886266472466698 36.420037665531076 33.21618476456562 5 14.315482319779862 36.82021532376724 33.97922192640086 14.885080430052033 6 32.46833291565452 35.56544199930028 17.31731100860749 14.648914076437705 7 28.98275772230231 32.159267134963194 20.55039315569164 18.30758198704286 8 26.001473868607345 35.779971763258 19.571188598112755 18.64736577002191 9 26.070651405261764 17.488319467223793 20.56458596450309 35.87644316301136 10-11 25.186503929075656 26.699725324486813 26.87897156723843 21.234799179199097 12-13 25.928202252488084 23.770026872048852 27.68954967607147 22.612221199391595 14-15 22.749746412556497 25.26202381637688 25.833116634343778 26.155113136722836 16-17 22.543607904284688 28.541620865691776 26.50721393269378 22.407557297329756 18-19 22.495056088888667 27.069457919066252 28.675205510382835 21.760280481662246 20-21 23.027509732743457 26.343118596797687 28.705129509380406 21.924242161078457 22-23 22.84357390805938 26.198436309770457 28.598360879457896 22.359628902712267 24-25 22.835868520929935 26.347101688051833 28.590707973384998 22.226321817633234 26-27 22.46190997747427 26.662196921235378 28.53013529830092 22.345757802989436 28-29 22.35584366861462 26.81648192847615 28.62514788318618 22.20252651972305 30-31 22.506593949198678 26.45690919331747 28.543797965862822 22.492698891621032 32-33 22.296461548272063 26.540608688035782 28.5128389123244 22.650090851367757 34-35 22.874993206678525 26.631606923902147 28.294934626533585 22.19846524288575 36-37 22.802175164800254 26.36657632435867 28.25986815979668 22.571380351044397 38-39 22.49821684991212 26.65664197969616 28.4945894982511 22.35055167214062 40-41 22.716524481287436 26.418365227541685 28.40529619961919 22.459814091551685 42-43 22.557463991018974 26.531231605914694 28.140009945833917 22.77129445723242 44-45 22.64640192076878 26.717092802501607 28.04593388722183 22.59057138950778 46-47 22.793466460710054 26.910489519165754 27.668682808206007 22.62736121191819 48-49 22.854018494426537 26.928901393744393 27.61588544542179 22.601194666407284 50-51 22.74414318382125 27.04214406517234 27.544529072675733 22.669183678330672 52-53 22.964907535835604 26.990777155532836 27.401971609419856 22.642343699211704 54-55 22.684326623178443 26.867855521176313 27.546529833433 22.901288022212242 56-57 22.694287738995108 27.221349369561075 27.440593636854477 22.64376925458934 58-59 22.76918641120516 26.830679003033143 27.52606358482476 22.874071000936937 60-61 22.835342591179614 26.801005931621958 27.741083026578778 22.622568450619653 62-63 22.66220065918191 26.93795040230543 27.691421677670814 22.70842726084185 64-65 22.504987494044784 26.778674368747023 27.64644870573289 23.069889431475303 66-67 22.45905576911434 27.047112291556868 27.441617065116734 23.05221487421206 68-69 22.52953098805857 26.83937782692027 27.64950109230661 22.981590092714548 70-71 22.483656530452684 26.879713007012302 27.506678943963863 23.129951518571147 72-73 22.572645097898125 26.82572372159267 27.4343024308907 23.167328749618505 74-75 22.44252780872461 27.033578895392544 27.245701836876986 23.27819145900586 76-77 22.500821499721944 26.842835615856547 27.45709138678343 23.19925149763808 78-79 22.535277328529514 26.834047594644687 27.39241968934162 23.238255387484177 80-81 22.26133259225992 27.078996275609835 27.406747572695085 23.252923559435164 82-83 22.48217834802653 26.937727810709617 27.296915049091737 23.283178792172123 84-85 22.391314596509858 26.839990968212575 27.268901620511087 23.499792814766476 86-87 22.40428199823935 26.814975666851478 27.385721638319392 23.395020696589782 88-89 22.273525093665807 26.737090863061034 27.297117216069754 23.6922668272034 90-91 22.248594877392073 26.74720501769875 27.414337670492987 23.58986243441619 92-93 22.339902883472572 26.569483972299214 27.376712379751922 23.713900764476293 94-95 22.16016038866461 26.46911699390354 27.357854801610838 24.01286781582101 96-97 22.241595351110735 26.340066646651167 27.40095429270435 24.017383709533746 98-99 21.98337850887945 26.350676045182826 27.30452354272885 24.361421903208868 100-101 22.38473961968646 25.40416718669863 27.96920054359572 24.241892650019192 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 229.0 1 166.0 2 129.5 3 178.0 4 167.0 5 154.0 6 282.5 7 616.0 8 1018.0 9 1276.5 10 1270.0 11 1252.0 12 1376.0 13 1527.0 14 1720.5 15 1866.0 16 1985.0 17 2087.5 18 2226.5 19 2456.5 20 2772.0 21 3114.5 22 3678.0 23 4541.5 24 5648.0 25 7281.0 26 9604.0 27 12337.0 28 14872.0 29 17622.5 30 20882.0 31 24686.0 32 29061.0 33 32526.0 34 35771.0 35 39392.5 36 43897.5 37 48815.5 38 52174.5 39 54394.0 40 56479.5 41 60411.5 42 64649.5 43 68358.0 44 72388.0 45 75422.0 46 76240.5 47 78393.0 48 84111.5 49 85304.0 50 82564.5 51 80197.5 52 76859.5 53 74731.5 54 72553.0 55 66960.0 56 59919.5 57 53145.5 58 46843.5 59 41250.0 60 34895.0 61 28231.5 62 22588.5 63 17207.5 64 12730.5 65 9578.5 66 7006.0 67 5520.5 68 4208.5 69 2932.0 70 2164.0 71 1621.0 72 1303.0 73 1061.5 74 817.5 75 630.0 76 405.0 77 217.0 78 127.0 79 67.0 80 34.5 81 13.5 82 10.5 83 6.0 84 1.5 85 1.0 86 0.0 87 0.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4379424397239846 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010421293183233628 16-17 0.002282759459184509 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.962520563444585E-5 26-27 0.0037715156282178844 28-29 4.962520563444585E-5 30-31 0.0 32-33 2.4812602817222926E-5 34-35 0.012828115656504251 36-37 0.027045737070772985 38-39 0.02044558472139169 40-41 0.009354351262093042 42-43 0.009478414276179157 44-45 0.0038955786423039993 46-47 0.005830961662047387 48-49 0.003250450969056203 50-51 8.436284957855794E-4 52-53 0.0 54-55 0.0 56-57 4.962520563444585E-5 58-59 0.001637631785936713 60-61 0.0028534493239806362 62-63 0.0015631939774850441 64-65 0.00322563836623898 66-67 0.002332384664818955 68-69 0.005111396180347922 70-71 1.7368821972056048E-4 72-73 0.0 74-75 0.0 76-77 0.10016847757312894 78-79 0.01585525320020545 80-81 4.962520563444585E-4 82-83 0.0 84-85 0.0 86-87 0.10982058006902866 88-89 0.42196312350969306 90-91 0.4271489574984927 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.4887561690333755E-4 100-101 2.233134253550063E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2015105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.512890388591934 #Duplication Level Percentage of deduplicated Percentage of total 1 78.2014642542146 45.757937061342496 2 12.781974869184628 14.95820588940674 3 3.8620016375073147 6.779306354880843 4 1.7222464076078279 4.030944610820123 5 0.889014441419369 2.6009402282323415 6 0.5500584871786076 1.931130718055794 7 0.3696494218411621 1.514047927167881 8 0.2533472959403438 1.1859266046082797 9 0.1914248151526974 1.008073730401763 >10 1.060823706253343 11.296255531646475 >50 0.0732630551624565 2.9734486275558343 >100 0.04193719192489926 4.407074198294351 >500 0.00237102015625445 0.8538658693608236 >1k 4.2339645647400604E-4 0.7028426482262644 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4920 0.24415601172147358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4256 0.21120487518020153 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2622 0.130117289173517 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4812602817222923E-5 0.0 18-19 2.4812602817222923E-5 0.0 0.0 4.9625205634445846E-5 0.0 20-21 4.9625205634445846E-5 0.0 0.0 7.443780845166876E-5 0.0 22-23 4.9625205634445846E-5 0.0 0.0 1.4887561690333755E-4 0.0 24-25 4.9625205634445846E-5 0.0 0.0 3.2256383662389803E-4 0.0 26-27 4.9625205634445846E-5 0.0 0.0 6.947528788822418E-4 0.0 28-29 4.9625205634445846E-5 0.0 0.0 0.0027790115155289672 0.0 30-31 4.9625205634445846E-5 0.0 0.0 0.008461097560673017 0.0 32-33 4.9625205634445846E-5 0.0 0.0 0.01900645375799276 0.0 34-35 4.9625205634445846E-5 0.0 0.0 0.03530833380890822 0.0 36-37 4.9625205634445846E-5 0.0 0.0 0.06143600457544396 0.0 38-39 4.9625205634445846E-5 0.0 0.0 0.0996970381196017 0.0 40-41 4.9625205634445846E-5 0.0 0.0 0.14520335168638854 0.0 42-43 4.9625205634445846E-5 0.0 0.0 0.1897419737433037 0.0 44-45 4.9625205634445846E-5 0.0 0.0 0.24892003146238034 2.4812602817222923E-5 46-47 4.9625205634445846E-5 0.0 0.0 0.3195366990801968 4.9625205634445846E-5 48-49 4.9625205634445846E-5 0.0 0.0 0.40040097166152633 4.9625205634445846E-5 50-51 4.9625205634445846E-5 0.0 0.0 0.4869969554936343 9.925041126889169E-5 52-53 4.9625205634445846E-5 0.0 0.0 0.5702680505482345 9.925041126889169E-5 54-55 4.9625205634445846E-5 0.0 0.0 0.6713545944256006 1.4887561690333755E-4 56-57 4.9625205634445846E-5 0.0 0.0 0.7880482654750001 1.4887561690333755E-4 58-59 4.9625205634445846E-5 0.0 0.0 0.9235250768570373 1.4887561690333755E-4 60-61 4.9625205634445846E-5 0.0 0.0 1.0756263321266137 1.4887561690333755E-4 62-63 4.9625205634445846E-5 0.0 0.0 1.2323427315201938 1.4887561690333755E-4 64-65 4.9625205634445846E-5 0.0 0.0 1.3930539599673466 1.4887561690333755E-4 66-67 4.9625205634445846E-5 0.0 0.0 1.583441061383898 1.4887561690333755E-4 68-69 4.9625205634445846E-5 0.0 0.0 1.8074492396177866 1.4887561690333755E-4 70-71 4.9625205634445846E-5 0.0 0.0 2.047833735711042 1.4887561690333755E-4 72-73 4.9625205634445846E-5 0.0 0.0 2.3003764071847375 1.4887561690333755E-4 74-75 4.9625205634445846E-5 0.0 0.0 2.558675602512028 1.4887561690333755E-4 76-77 4.9625205634445846E-5 0.0 0.0 2.847643174921406 1.4887561690333755E-4 78-79 4.9625205634445846E-5 0.0 0.0 3.1739040893650703 1.4887561690333755E-4 80-81 4.9625205634445846E-5 0.0 0.0 3.5251016696400437 1.4887561690333755E-4 82-83 4.9625205634445846E-5 0.0 0.0 3.8976380883378283 1.4887561690333755E-4 84-85 4.9625205634445846E-5 0.0 0.0 4.282779309266763 1.7368821972056045E-4 86-87 4.9625205634445846E-5 0.0 0.0 4.6849667883311295 2.233134253550063E-4 88-89 4.9625205634445846E-5 0.0 0.0 5.134571151379209 2.4812602817222924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4405 0.0 38.304695 1 GTATCAA 7120 0.0 34.945007 1 TATAGCG 85 1.6448099E-5 33.49356 5 ATCAACG 8685 0.0 27.972359 3 TCAACGC 8905 0.0 27.174728 4 TATCAAC 9030 0.0 27.061287 2 CAACGCA 8990 0.0 26.917793 5 AACGCAG 9180 0.0 26.464046 6 ACGCAGA 10470 0.0 23.112795 7 CGCAGAG 10460 0.0 23.089529 8 GTACATG 7075 0.0 22.299555 1 GAGTACT 5580 0.0 21.3447 12-13 TACATGG 7250 0.0 21.008547 2 GCAGAGT 11585 0.0 20.765429 9 ACATGGG 7230 0.0 20.01661 3 AGAGTAC 9700 0.0 19.88563 10-11 AGTACTT 6125 0.0 19.135569 12-13 ATAACGC 150 7.7795895E-4 18.979681 3 GTACTTT 6250 0.0 18.828781 14-15 ATAGCGT 155 9.697376E-4 18.367435 6 >>END_MODULE