FastQCFastQC Report
Fri 27 May 2016
SRR939137_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939137_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887329
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121930.6460452841025598No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104600.5542223957773128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93450.49514419584502756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63870.3384147649932789No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58910.31213423838663No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44180.23408743255680384No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38470.20383303599955283No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30710.16271672824398925No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24380.12917726586090714No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23310.12350787806471474No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23260.12324295340134127No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22780.12069967663295589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22130.11725565600910069No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22040.11677879161502844No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19920.10554598588799304No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19900.10544001602264363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.10014152275517411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG121750.026.3553331
TACATGG123500.025.5055312
ACATGGG125950.024.0691913
CATGGGG40400.023.0974774
GAGTACT90100.022.33393912-13
GTGTAAG5950.021.5395981
CATGGGA85100.020.9841564
AGTACTT92850.020.85655612-13
GTACTTT96900.020.76857214-15
CCTATAC5450.019.989923
ATGGGAG30850.018.732015
AGAGTAC177900.018.56415410-11
GTATCAA253300.018.2334331
ATGGGGA22550.018.0647345
TACTTTT107700.017.91650814-15
ATGGGAT34250.017.8405745
ACTTTTT113550.017.80924816-17
CCCTATA4103.45608E-1117.3295442
TGGACCG1650.001491952217.2245165
GCACCGT3652.39379E-916.863436
Produced by FastQC (version 0.11.5)