##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939137_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887329 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.001670614927235 33.0 31.0 34.0 30.0 34.0 2 32.08686614787353 34.0 31.0 34.0 30.0 34.0 3 32.1332163072787 34.0 31.0 34.0 30.0 34.0 4 35.59143795278936 37.0 35.0 37.0 33.0 37.0 5 35.648743276874356 37.0 35.0 37.0 33.0 37.0 6 35.74598122531896 37.0 36.0 37.0 35.0 37.0 7 35.67025674908826 37.0 35.0 37.0 33.0 37.0 8 35.66244200136807 37.0 35.0 37.0 33.0 37.0 9 37.310527735227936 39.0 37.0 39.0 34.0 39.0 10-11 37.222326367051 39.0 37.0 39.0 34.0 39.0 12-13 37.12784416495481 39.0 37.0 39.0 34.0 39.0 14-15 38.373945136221614 40.0 38.0 41.0 34.0 41.0 16-17 38.32213090563437 40.0 38.0 41.0 33.5 41.0 18-19 38.296654690305715 40.0 38.0 41.0 33.5 41.0 20-21 38.213605576982076 40.0 38.0 41.0 33.5 41.0 22-23 38.12328825551877 40.0 38.0 41.0 33.0 41.0 24-25 38.146694614452485 40.0 38.0 41.0 33.0 41.0 26-27 38.05584929813509 40.0 38.0 41.0 33.0 41.0 28-29 37.93727908594633 40.0 38.0 41.0 33.0 41.0 30-31 37.752177548270595 40.0 37.5 41.0 33.0 41.0 32-33 37.51126406683731 40.0 37.0 41.0 32.5 41.0 34-35 37.17749555059028 40.0 36.5 41.0 31.5 41.0 36-37 36.91374105945492 40.0 36.0 41.0 31.0 41.0 38-39 36.67102847463267 39.5 35.5 41.0 30.0 41.0 40-41 36.52080930245866 40.0 35.5 41.0 30.0 41.0 42-43 36.29786301169537 39.0 35.0 41.0 30.0 41.0 44-45 35.98673045346095 39.0 35.0 41.0 29.0 41.0 46-47 35.54108663619327 39.0 35.0 41.0 26.5 41.0 48-49 35.43367770007243 39.0 35.0 41.0 26.0 41.0 50-51 34.41491308616568 38.0 34.0 40.0 24.0 40.5 52-53 34.58677342424134 38.5 34.5 39.5 24.0 40.5 54-55 35.10133341881569 39.0 35.0 41.0 25.0 41.0 56-57 34.987792536436416 39.0 35.0 41.0 24.5 41.0 58-59 34.802165918077876 39.0 35.0 41.0 24.0 41.0 60-61 34.500858620833995 38.0 34.5 40.5 23.0 41.0 62-63 34.15509987924734 38.0 34.0 40.0 22.0 41.0 64-65 33.78918116555195 37.0 34.0 40.0 21.5 41.0 66-67 33.387450200786404 37.0 33.5 40.0 20.0 41.0 68-69 32.973791532901785 36.0 33.0 39.0 18.5 41.0 70-71 32.550570939142034 36.0 33.0 39.0 17.0 41.0 72-73 32.100482215872276 35.0 32.0 38.5 12.5 40.0 74-75 31.65532850923183 35.0 32.0 37.0 8.0 39.5 76-77 31.221393302386602 35.0 31.5 37.0 7.0 39.0 78-79 30.78928448617067 35.0 31.0 36.0 2.0 39.0 80-81 30.345349962831072 35.0 31.0 36.0 2.0 37.5 82-83 29.920369209607863 34.5 30.5 35.5 2.0 37.0 84-85 29.535647467929543 34.0 30.0 35.0 2.0 37.0 86-87 29.167919053858654 34.0 30.0 35.0 2.0 36.0 88-89 28.75959808809169 34.0 29.0 35.0 2.0 36.0 90-91 28.372374132967806 34.0 29.0 35.0 2.0 36.0 92-93 27.954920949129697 34.0 28.5 35.0 2.0 35.0 94-95 27.49325316359787 34.0 27.0 35.0 2.0 35.0 96-97 26.931990129966742 34.0 25.5 35.0 2.0 35.0 98-99 26.246148657706208 33.5 24.5 35.0 2.0 35.0 100-101 23.756931621354838 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3337530465709051 1101 2 0.37996738410273423 1101 3 0.3443403238378977 1101 4 0.3166044661683216 1101 5 0.3989462777541277 1101 6 0.39726666793249166 1101 7 0.3599055909510369 1101 8 0.4561491275062721 1101 9 0.49559321758122366 1101 10-11 0.4826626253210762 1101 12-13 0.4590121011580237 1101 14-15 0.49755368565198665 1101 16-17 0.592628826677128 1101 18-19 0.52424814718308 1101 20-21 0.7689798039963094 1101 22-23 0.7084205984683933 1101 24-25 0.6330694274998692 1101 26-27 0.6307140654369334 1101 28-29 0.7548769185642783 1101 30-31 0.5765456567404286 1101 32-33 0.5483613422658777 1101 34-35 0.4961015229052279 1101 36-37 0.4652401335812755 1101 38-39 0.5136231002256082 1101 40-41 0.4072126596726733 1101 42-43 0.4450953116924623 1101 44-45 0.38424194047523486 1101 46-47 0.2962862169164495 1101 48-49 0.08611143278971412 1101 50-51 -0.15600282810041932 1101 52-53 -0.03239055236173982 1101 54-55 0.0030138453455563763 1101 56-57 -0.04742071965279138 1101 58-59 0.04045976574718679 1101 60-61 -0.06879104586271012 1101 62-63 0.02163380846693741 1101 64-65 -0.06736593328760421 1101 66-67 -0.12610663922104237 1101 68-69 -0.06927827405254305 1101 70-71 0.0559896127654298 1101 72-73 -0.12175224162503184 1101 74-75 -0.25811932519517455 1101 76-77 -0.4228623333502739 1101 78-79 -0.2728786437615405 1101 80-81 -0.4033300224068519 1101 82-83 -0.34198621331410095 1101 84-85 -0.2621545791269213 1101 86-87 -0.3177274855064276 1101 88-89 -0.3658537706999532 1101 90-91 -0.39172581128259054 1101 92-93 -0.4922005446884974 1101 94-95 -0.5186835555881402 1101 96-97 -0.3599779107873857 1101 98-99 -0.4762260969437513 1101 100-101 -0.4577675800622103 1102 1 0.30386886337949903 1102 2 0.3353956408686187 1102 3 0.43479548963994574 1102 4 0.38929392380813255 1102 5 0.3989098312126913 1102 6 0.4114982500491209 1102 7 0.3929954851570301 1102 8 0.4299670413775445 1102 9 0.48328339820948685 1102 10-11 0.34223469538559215 1102 12-13 0.5332111190821891 1102 14-15 0.5205736381526336 1102 16-17 0.6689225303390174 1102 18-19 0.6486580202342296 1102 20-21 0.7158556025024012 1102 22-23 0.752841009323916 1102 24-25 0.6614701968388275 1102 26-27 0.7002447567921735 1102 28-29 0.5873280078998988 1102 30-31 0.6183347857671251 1102 32-33 0.7338179837567012 1102 34-35 0.7604399686583108 1102 36-37 0.6912427601092119 1102 38-39 0.645248992324035 1102 40-41 0.6515887008255774 1102 42-43 0.7432785308332299 1102 44-45 0.777200032894946 1102 46-47 0.6931842993133657 1102 48-49 0.7258173646624186 1102 50-51 0.8402512993713529 1102 52-53 0.8407136827659869 1102 54-55 0.837122063842564 1102 56-57 0.7919805666724287 1102 58-59 0.7839416781606019 1102 60-61 0.623303025830019 1102 62-63 0.7520510223269312 1102 64-65 0.7646016451368354 1102 66-67 0.6220665769287379 1102 68-69 0.37241260777548746 1102 70-71 0.33253329216262983 1102 72-73 0.2881881563949662 1102 74-75 0.28293057289316437 1102 76-77 0.3630320627959023 1102 78-79 0.2672707870583686 1102 80-81 0.2576497953208232 1102 82-83 0.15110795939892796 1102 84-85 0.2486415203008292 1102 86-87 0.04421816556943625 1102 88-89 0.10010872501658596 1102 90-91 -0.006624721649846066 1102 92-93 0.023890009120659528 1102 94-95 0.017284228838491345 1102 96-97 -0.10957729595007493 1102 98-99 -0.1735734225797323 1102 100-101 -0.47202649708492217 1103 1 0.19613833555806792 1103 2 0.19214138507895484 1103 3 0.40364565008486863 1103 4 0.2742647990563256 1103 5 0.23396116417588786 1103 6 0.2737301752940269 1103 7 0.21606755833120417 1103 8 0.25793433131703836 1103 9 0.33281335576921833 1103 10-11 0.3747847814894598 1103 12-13 0.3711137605419097 1103 14-15 0.38114653472448623 1103 16-17 0.44424883107207336 1103 18-19 0.4977061725298597 1103 20-21 0.48410444739310066 1103 22-23 0.5595147650524126 1103 24-25 0.4602393349531937 1103 26-27 0.45422294381165784 1103 28-29 0.3772816153227154 1103 30-31 0.46083527980572825 1103 32-33 0.4485252776484998 1103 34-35 0.3914994697263765 1103 36-37 0.3842198837694255 1103 38-39 0.34209185040792534 1103 40-41 0.42999403832451577 1103 42-43 0.3674638376547321 1103 44-45 0.3203705373789347 1103 46-47 0.3788816465613323 1103 48-49 0.4047139176845178 1103 50-51 0.3466267498875055 1103 52-53 0.2913549919212315 1103 54-55 0.27757488550874854 1103 56-57 0.2615214401326753 1103 58-59 0.17677388383122405 1103 60-61 0.22461575696950575 1103 62-63 0.12831082075917521 1103 64-65 0.08359813511020064 1103 66-67 0.18384869179035235 1103 68-69 0.013760345078694058 1103 70-71 0.22024819895401748 1103 72-73 -0.15766050781876118 1103 74-75 -0.04892785916947062 1103 76-77 0.03056290491998581 1103 78-79 0.1106727839153443 1103 80-81 0.08284670499174851 1103 82-83 0.056931968733906047 1103 84-85 -0.13526975977671185 1103 86-87 -0.09105619053358538 1103 88-89 -0.1742426728773978 1103 90-91 -0.3513243932318062 1103 92-93 -0.6976030225825021 1103 94-95 -0.7316340618820512 1103 96-97 -0.6786410692757556 1103 98-99 -0.8752757384444898 1103 100-101 -0.652954725039038 1104 1 0.2599623055410518 1104 2 0.3635408702522369 1104 3 0.3847603081032176 1104 4 0.36392099951879686 1104 5 0.31431230799287846 1104 6 0.29385822753293667 1104 7 0.22745228222992608 1104 8 0.29504584801982503 1104 9 0.35978213167416584 1104 10-11 0.4172558043076222 1104 12-13 0.42308235465776534 1104 14-15 0.3355680091067583 1104 16-17 0.36269188018422227 1104 18-19 0.34387333123900987 1104 20-21 0.42280043785281407 1104 22-23 0.40969437267882824 1104 24-25 0.44439659706398515 1104 26-27 0.49065075172602945 1104 28-29 0.4232729839764602 1104 30-31 0.37918125354084253 1104 32-33 0.316543849222505 1104 34-35 0.40677757068476694 1104 36-37 0.4250346261379221 1104 38-39 0.5757408302075149 1104 40-41 0.37748934816584523 1104 42-43 0.39246583733478246 1104 44-45 0.2329592504575686 1104 46-47 0.17843971727001673 1104 48-49 0.2705190252309535 1104 50-51 0.44968698752220604 1104 52-53 0.31268103429990646 1104 54-55 0.23313599573111077 1104 56-57 0.2248753035145299 1104 58-59 0.3577138425287387 1104 60-61 0.3857748806006285 1104 62-63 0.38799599840347554 1104 64-65 0.3805248086606099 1104 66-67 0.3571144238186932 1104 68-69 0.3615168858503637 1104 70-71 0.3274214148758361 1104 72-73 0.294767103763391 1104 74-75 0.34553611750335733 1104 76-77 0.31585362204160816 1104 78-79 0.2144316370442958 1104 80-81 0.31079150655077115 1104 82-83 0.26088842660973555 1104 84-85 0.29082770099320143 1104 86-87 0.3018736679617824 1104 88-89 0.3819057979127294 1104 90-91 0.11762239346414205 1104 92-93 0.04118877073056737 1104 94-95 -0.12128880803176045 1104 96-97 -0.169421089926999 1104 98-99 -0.18892664520849678 1104 100-101 -0.2361659566965173 1105 1 0.10914492117103691 1105 2 0.16848172617993384 1105 3 0.194801509591791 1105 4 0.230243212880481 1105 5 0.155960699805739 1105 6 0.25223863245939526 1105 7 0.24978083087426484 1105 8 0.2430206619485844 1105 9 0.17301866366069873 1105 10-11 0.17932136119225817 1105 12-13 0.26900457023458557 1105 14-15 0.3028670964716369 1105 16-17 0.15967348349806088 1105 18-19 0.31637244518550034 1105 20-21 0.2963038380831833 1105 22-23 0.31299824473269666 1105 24-25 0.15653580714727156 1105 26-27 0.11600932650894435 1105 28-29 0.24270081491744122 1105 30-31 0.1583850236985569 1105 32-33 0.2115063580830423 1105 34-35 0.2262095550016241 1105 36-37 0.3402406889591134 1105 38-39 0.3628152476273314 1105 40-41 0.191902669980486 1105 42-43 0.19264554006382895 1105 44-45 0.06214889913735533 1105 46-47 0.11997541415110646 1105 48-49 0.07807789241152818 1105 50-51 0.28544035452557637 1105 52-53 0.2757630607042998 1105 54-55 0.2814425259455362 1105 56-57 0.21179355400186495 1105 58-59 0.13512058539600957 1105 60-61 0.14175435738602715 1105 62-63 0.14416237710275226 1105 64-65 0.02314066015804883 1105 66-67 0.05382522702620207 1105 68-69 -0.17013322269119868 1105 70-71 -0.24509237433203168 1105 72-73 -0.07642563502803057 1105 74-75 -0.21136928520796872 1105 76-77 -0.026503612585361935 1105 78-79 -0.010839163062030366 1105 80-81 -0.24359002809392294 1105 82-83 -0.131451917622055 1105 84-85 -0.06360478172875261 1105 86-87 -0.03021226815375755 1105 88-89 -0.13759014839242667 1105 90-91 -0.12154560228280431 1105 92-93 -0.02391539593610048 1105 94-95 -0.017325656782421817 1105 96-97 -0.05241910738224931 1105 98-99 -0.0052851313860635685 1105 100-101 0.0042978923199612495 1106 1 0.05488462154556828 1106 2 0.13028728173549098 1106 3 0.14809426864297848 1106 4 0.22568380796995768 1106 5 0.24055417670960821 1106 6 0.21039900491049934 1106 7 0.07908794697912924 1106 8 0.18335696032561088 1106 9 0.28588019091829153 1106 10-11 0.2557161021410721 1106 12-13 0.2491797418950128 1106 14-15 0.2474181784524987 1106 16-17 0.19940832016056476 1106 18-19 0.19009799657958837 1106 20-21 0.2978676385409429 1106 22-23 0.26906618064613497 1106 24-25 0.180213891833084 1106 26-27 0.362389502330835 1106 28-29 0.36210624562905736 1106 30-31 0.2854826620672668 1106 32-33 0.12135407447921409 1106 34-35 0.14050223602534118 1106 36-37 0.1770351906236982 1106 38-39 0.2557466094666978 1106 40-41 0.3839607744332554 1106 42-43 0.24921480244418603 1106 44-45 0.3578658415010665 1106 46-47 0.3675364056201076 1106 48-49 0.42697835537448725 1106 50-51 0.29246022552016626 1106 52-53 0.3257851684862416 1106 54-55 0.3261652683508629 1106 56-57 0.4812620569148862 1106 58-59 0.6001772853543983 1106 60-61 0.5994979331246881 1106 62-63 0.5170569359874762 1106 64-65 0.4464110513357369 1106 66-67 0.4419956130020637 1106 68-69 0.6228865702759236 1106 70-71 0.6205111681149091 1106 72-73 0.6276174881513299 1106 74-75 0.5298467728444649 1106 76-77 0.5106361606148013 1106 78-79 0.5971469480490832 1106 80-81 0.5461155482689577 1106 82-83 0.5774802765102791 1106 84-85 0.6426374155246783 1106 86-87 0.5917492122873576 1106 88-89 0.8588478977998335 1106 90-91 0.8848526499019655 1106 92-93 0.7121157370227031 1106 94-95 0.45290881575190767 1106 96-97 0.47870669890156137 1106 98-99 0.46707168821443545 1106 100-101 0.5014576925696517 1107 1 0.08238545773847505 1107 2 0.2022277448005383 1107 3 0.13104584378265116 1107 4 0.23191871004247844 1107 5 0.2354447283491652 1107 6 0.1996263626647945 1107 7 0.18009137846706125 1107 8 0.2097397756322792 1107 9 0.2995435830122446 1107 10-11 0.4087071951381134 1107 12-13 0.337173250560447 1107 14-15 0.48064972336534595 1107 16-17 0.44867154129951814 1107 18-19 0.4170732700456341 1107 20-21 0.3105560916460135 1107 22-23 0.4714864945591515 1107 24-25 0.4805821264130614 1107 26-27 0.44314312893044416 1107 28-29 0.43145265247716935 1107 30-31 0.4246697586816097 1107 32-33 0.4583311442115061 1107 34-35 0.4641974653899581 1107 36-37 0.5032516964522742 1107 38-39 0.545708268473426 1107 40-41 0.43196134428323063 1107 42-43 0.4397261808617401 1107 44-45 0.557392618256138 1107 46-47 0.6546590348583621 1107 48-49 0.570611210942296 1107 50-51 0.7319737470691692 1107 52-53 0.7286443288246787 1107 54-55 0.7946640233525315 1107 56-57 0.7685342717731132 1107 58-59 0.7293861229843159 1107 60-61 0.6412601941903091 1107 62-63 0.5970257800591838 1107 64-65 0.6449415197276522 1107 66-67 0.5417410387171699 1107 68-69 0.7181657967665132 1107 70-71 0.7551153081989028 1107 72-73 0.8047116868307569 1107 74-75 0.8439096793271261 1107 76-77 0.8369765468739843 1107 78-79 0.9156144232420225 1107 80-81 1.0246349913025412 1107 82-83 0.7806562610500016 1107 84-85 0.6834857487134833 1107 86-87 0.7230726888677594 1107 88-89 0.8757646997026391 1107 90-91 0.8197596776387961 1107 92-93 0.7658993550745556 1107 94-95 0.7998706481912379 1107 96-97 0.7867775089556943 1107 98-99 0.8785074004649722 1107 100-101 0.7940657472752477 1108 1 0.02614724841772187 1108 2 0.1187405577827505 1108 3 0.0829221087086296 1108 4 0.05318474329381928 1108 5 0.1533294938145744 1108 6 0.07887678040863477 1108 7 0.2764099035559653 1108 8 0.2544126090783152 1108 9 0.22810553759155994 1108 10-11 0.20692478194652608 1108 12-13 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68-69 -2.122601383283971 2316 70-71 -1.887687892553707 2316 72-73 -1.8948974861948606 2316 74-75 -1.952567779864772 2316 76-77 -2.1372943316336332 2316 78-79 -1.7706500104383842 2316 80-81 -1.6852572656150464 2316 82-83 -1.8052717473932596 2316 84-85 -1.815625606499271 2316 86-87 -1.7570494918602044 2316 88-89 -1.9293745537904279 2316 90-91 -1.9691445884452925 2316 92-93 -2.0165845543824616 2316 94-95 -1.8616161845944532 2316 96-97 -2.007671610579038 2316 98-99 -1.998380289969429 2316 100-101 -1.8116871741274991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6511.0 3 1762.0 4 1746.0 5 1850.0 6 2112.0 7 2530.0 8 3154.0 9 3880.0 10 4593.0 11 5908.0 12 7727.0 13 11880.0 14 10544.0 15 11507.0 16 14742.0 17 21022.0 18 19784.0 19 11546.0 20 11223.0 21 11754.0 22 12342.0 23 13676.0 24 14961.0 25 16821.0 26 19459.0 27 22542.0 28 26045.0 29 31273.0 30 36925.0 31 44984.0 32 56998.0 33 74021.0 34 102551.0 35 154574.0 36 242024.0 37 386730.0 38 390470.0 39 75137.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23219135951787 19.35982409197744 14.653774117044119 21.75421043146057 2 14.89035854350355 21.350337568477837 40.5474725730585 23.211831314960115 3 19.778307276278337 26.072526206462687 30.066538582723943 24.08262793453503 4 12.428086797147078 16.959129163936904 37.975210666893005 32.63757337202301 5 13.0711141318333 38.48598149683859 33.64313421503758 14.79977015629054 6 30.117071949717822 36.504323643803446 17.942936174680938 15.435668231797795 7 27.491855088189922 34.1164540742591 20.980380613863563 17.411310223687412 8 25.89509895334443 34.02877003384897 20.22011908133618 19.856011931470423 9 26.763188848808312 17.54109732155452 21.327810204036176 34.36790362560099 10-11 24.844770069850487 26.98164923299166 26.860975976449758 21.312604720708098 12-13 26.604327634183868 24.762953813713704 27.577305509166035 21.055413042936397 14-15 23.13009610083733 25.66253785655967 27.33319844938259 23.87416759322041 16-17 21.158493822806896 29.252863886985008 27.727569809109553 21.861072481098546 18-19 21.095036961057502 27.716072888966202 30.53214588400518 20.65674426597111 20-21 22.53562663467205 26.19548861013824 30.14254366772272 21.126341087466987 22-23 21.845987364534764 26.4082284356318 30.262787971376586 21.482996228456848 24-25 22.075098578323512 26.516289131250755 30.28408329785442 21.124528992571314 26-27 21.5911481274283 26.35515982120334 30.87316827189486 21.180523779473504 28-29 20.957695491676418 27.274791376608025 30.68841409087521 21.079099040840347 30-31 21.314896366323325 26.650012359444474 30.416168487341917 21.61892278689029 32-33 21.170244505942588 26.83295917298408 30.39158875158626 21.60520756948708 34-35 22.006131944453674 26.65612337248971 30.11446562110856 21.223279061948055 36-37 21.822998572762366 26.259042699190964 30.531064981243045 21.386893746803622 38-39 21.464245686494586 26.912781848025034 30.403429565174804 21.219542900305573 40-41 21.495709085838772 26.979207606483886 30.292967882073103 21.232115425604242 42-43 21.287446420505105 27.01692211006119 29.58368037061319 22.111951098820512 44-45 21.514357102204208 26.853608295723348 29.504293580951398 22.127741021121043 46-47 22.015430200704422 27.342177213033143 29.106427386742702 21.535965199519737 48-49 21.82731818156403 27.29765342917035 29.01677156605706 21.85825682320856 50-51 21.273379563567282 27.631252475859043 29.314783380969217 21.780584579604458 52-53 21.7337676427091 28.037447868897846 28.34640742993833 21.882377058454722 54-55 21.17619217983692 27.47800698920953 28.786993085325 22.55880774562855 56-57 21.822001082633 27.671918554488002 28.42100584802792 22.085074514851076 58-59 22.30228040313562 27.13983863186987 28.512689054634365 22.045191910360145 60-61 21.840878987600338 27.424742937086744 29.304081621797017 21.430296453515897 62-63 21.54520449646827 27.497935898125192 29.187571631388405 21.769287974018134 64-65 21.246329497382423 27.86027635566648 28.87272138632045 22.020672760630646 66-67 21.583902903630275 27.579015813414014 28.31905557793689 22.51802570501881 68-69 21.33250105056171 27.512653343396 28.73564565205929 22.419199953983 70-71 21.761787446251248 27.53182320241911 28.536074106974528 22.170315244355116 72-73 21.628693236884764 27.18644096678616 28.922270290304237 22.26259550602484 74-75 21.136169496652375 28.250090001427242 28.308590929134258 22.305149572786124 76-77 21.362379352057722 27.405132169661034 28.51675973489398 22.715728743387256 78-79 21.37044433283535 27.533502024533952 28.58091521763374 22.51513842499696 80-81 21.61857871311558 27.659050633551534 28.419429588025146 22.30294106530774 82-83 21.551467577845703 27.400003939734834 28.589218223509675 22.459310258909788 84-85 21.24792996169647 27.57156589263672 28.420584399664268 22.75991974600254 86-87 21.278705029574535 27.5433848155741 28.58245344463017 22.59545671022119 88-89 21.261820464719516 27.41615650324575 28.137644815502505 23.184378216532224 90-91 21.21427487278353 27.1155745977137 28.60854074521823 23.06160978428454 92-93 21.670223036865014 27.217468265586565 28.527636111704286 22.58467258584414 94-95 21.298373744307682 26.903995155902095 28.673001556651073 23.124629543139154 96-97 20.964415683524077 26.81888368682265 29.037461533689125 23.179239095964142 98-99 21.162176388878905 27.06073650194194 28.297975840444373 23.479111268734783 100-101 21.131584818279062 26.223865585234652 29.080860878950144 23.56368871753614 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3555.0 1 2160.5 2 959.5 3 1160.5 4 978.5 5 970.5 6 1685.5 7 3337.0 8 5178.0 9 5733.5 10 4880.5 11 4001.0 12 3880.5 13 4071.5 14 4249.5 15 4350.5 16 4410.0 17 4463.5 18 4593.0 19 4761.0 20 4999.0 21 5399.5 22 6122.5 23 7229.5 24 8324.5 25 9648.5 26 11724.5 27 14233.5 28 16261.0 29 18856.5 30 22185.5 31 25774.5 32 29882.0 33 33626.0 34 37806.0 35 41233.0 36 45558.0 37 50877.0 38 53170.5 39 53554.0 40 53466.0 41 55134.5 42 57374.0 43 59509.0 44 62840.0 45 65484.5 46 66359.0 47 72998.0 48 92667.5 49 92563.5 50 74059.5 51 64740.0 52 59005.0 53 57108.0 54 55697.0 55 51527.5 56 46018.0 57 40970.0 58 36954.0 59 32738.0 60 27941.0 61 23099.5 62 18672.0 63 14161.0 64 10477.0 65 7970.0 66 5820.0 67 4654.0 68 3564.0 69 2462.0 70 1773.0 71 1268.5 72 990.5 73 850.0 74 658.0 75 523.0 76 380.5 77 218.5 78 144.0 79 102.5 80 70.0 81 56.0 82 41.5 83 31.0 84 22.5 85 18.5 86 15.0 87 10.0 88 8.5 89 6.0 90 6.5 91 8.5 92 4.5 93 3.0 94 3.0 95 2.5 96 4.0 97 4.0 98 2.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33624238275361634 2 0.11815639986457051 3 0.111639253145583 4 0.11889818892201626 5 0.13617127697396691 6 0.08747812384592193 7 0.09516093908375275 8 0.10040644741854758 9 0.08917364169151218 10-11 0.08787551084098215 12-13 0.09145199379652408 14-15 0.09312101917577699 16-17 0.08273597237153671 18-19 0.09701541172736709 20-21 0.10660568454148693 22-23 0.11439446964466714 24-25 0.12750824048165424 26-27 0.1268724212895579 28-29 0.12152094308941366 30-31 0.11291089152977568 32-33 0.10578441808502916 34-35 0.3283211353187494 36-37 0.4888124963904015 38-39 0.4622140601877044 40-41 0.4398014336663083 42-43 0.473075971386017 44-45 0.48004349003273933 46-47 0.4871434710111485 48-49 0.49903858839661763 50-51 0.4870904860784738 52-53 0.49019010463994356 54-55 0.47712931873563114 56-57 0.4543987826181869 58-59 0.43497980479291104 60-61 0.39415491416705833 62-63 0.46563158834522234 64-65 0.49543561297473837 66-67 0.5046549912601354 68-69 0.5172389127703755 70-71 0.5092381879364966 72-73 0.5000188096510996 74-75 0.5076486399562556 76-77 0.4841233298486909 78-79 0.46353868350457184 80-81 0.4658435280759211 82-83 0.4784539420524985 84-85 0.4791162537109322 86-87 0.4826132592674621 88-89 0.4946673314509553 90-91 0.5099269920612676 92-93 0.502191191890762 94-95 0.507595655023581 96-97 0.5059796145770027 98-99 0.5000982870501116 100-101 0.4875673504725462 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.14553274590315 #Duplication Level Percentage of deduplicated Percentage of total 1 73.78315177848009 35.52329150046755 2 13.811579966956547 13.299317511435326 3 5.0680322043066335 7.320093313492102 4 2.3668467283562293 4.558123866584342 5 1.2982715456336027 3.125298760668845 6 0.8014570757660449 2.3151946731437936 7 0.5346104937162613 1.80173749220638 8 0.3614189313445084 1.392056559522908 9 0.28264187188714995 1.2247149148475527 >10 1.4863091307431706 13.380106669844515 >50 0.13195033560611297 4.403254406798849 >100 0.0674392842742426 5.8623301323626436 >500 0.0030901452986357844 0.9764001735847451 >1k 0.0026486959702592436 2.465495453553861 >5k 3.3108699628240545E-4 1.1489280013834862 >10k+ 2.207246641882703E-4 1.203656570103136 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12193 0.6460452841025598 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10460 0.5542223957773128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9345 0.49514419584502756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6387 0.3384147649932789 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5891 0.31213423838663 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4418 0.23408743255680384 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3847 0.20383303599955283 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3071 0.16271672824398925 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2438 0.12917726586090714 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2331 0.12350787806471474 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2326 0.12324295340134127 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2278 0.12069967663295589 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2213 0.11725565600910069 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2204 0.11677879161502844 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1992 0.10554598588799304 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1990 0.10544001602264363 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.10014152275517411 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.2984932674695294E-5 0.0 12-13 0.0 0.0 0.0 1.0596986534939059E-4 0.0 14-15 0.0 0.0 0.0 1.0596986534939059E-4 0.0 16-17 0.0 0.0 0.0 1.5895479802408588E-4 0.0 18-19 0.0 0.0 0.0 1.5895479802408588E-4 0.0 20-21 0.0 0.0 0.0 2.3843219703612882E-4 0.0 22-23 0.0 0.0 0.0 5.298493267469529E-4 0.0 24-25 0.0 0.0 0.0 9.80221254481863E-4 0.0 26-27 0.0 0.0 0.0 0.002013427441638421 0.0 28-29 0.0 0.0 0.0 0.005351478200144225 0.0 30-31 0.0 0.0 0.0 0.014729811283565292 0.0 32-33 0.0 0.0 0.0 0.03274468839296169 0.0 34-35 0.0 0.0 0.0 0.05746215948570705 0.0 36-37 0.0 5.2984932674695294E-5 0.0 0.10022100015418615 0.0 38-39 0.0 5.2984932674695294E-5 0.0 0.16478314061830235 0.0 40-41 0.0 5.2984932674695294E-5 0.0 0.23803481004106863 0.0 42-43 0.0 5.2984932674695294E-5 0.0 0.3084517855657387 0.0 44-45 0.0 5.2984932674695294E-5 0.0 0.3975194573919015 0.0 46-47 0.0 5.2984932674695294E-5 0.0 0.5033038754769306 0.0 48-49 0.0 5.2984932674695294E-5 0.0 0.6227848986583686 0.0 50-51 0.0 5.2984932674695294E-5 0.0 0.7573136427194199 0.0 52-53 0.0 5.2984932674695294E-5 0.0 0.8898554518051702 0.0 54-55 0.0 5.2984932674695294E-5 0.0 1.036226328319016 0.0 56-57 0.0 5.2984932674695294E-5 0.0 1.2130635411208115 0.0 58-59 0.0 5.2984932674695294E-5 0.0 1.4102204756033527 0.0 60-61 0.0 5.2984932674695294E-5 0.0 1.6325982380390487 0.0 62-63 0.0 5.2984932674695294E-5 0.0 1.8532539901628173 0.0 64-65 0.0 5.2984932674695294E-5 0.0 2.081301140394706 0.0 66-67 0.0 5.2984932674695294E-5 0.0 2.3427023057453153 0.0 68-69 0.0 5.2984932674695294E-5 0.0 2.6457761206445722 0.0 70-71 0.0 5.2984932674695294E-5 0.0 2.9824953677922608 0.0 72-73 0.0 5.2984932674695294E-5 0.0 3.3219433389726962 0.0 74-75 0.0 5.2984932674695294E-5 0.0 3.6808102879784075 0.0 76-77 0.0 5.2984932674695294E-5 0.0 4.066990969777924 0.0 78-79 0.0 5.2984932674695294E-5 0.0 4.516197228993992 0.0 80-81 0.0 5.2984932674695294E-5 0.0 5.01221567622815 0.0 82-83 0.0 5.2984932674695294E-5 0.0 5.518354245603178 0.0 84-85 0.0 5.2984932674695294E-5 0.0 6.035195771378493 0.0 86-87 0.0 5.2984932674695294E-5 0.0 6.563058163150146 0.0 88-89 0.0 5.2984932674695294E-5 0.0 7.132195817475385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12175 0.0 26.355333 1 TACATGG 12350 0.0 25.505531 2 ACATGGG 12595 0.0 24.069191 3 CATGGGG 4040 0.0 23.097477 4 GAGTACT 9010 0.0 22.333939 12-13 GTGTAAG 595 0.0 21.539598 1 CATGGGA 8510 0.0 20.984156 4 AGTACTT 9285 0.0 20.856556 12-13 GTACTTT 9690 0.0 20.768572 14-15 CCTATAC 545 0.0 19.98992 3 ATGGGAG 3085 0.0 18.73201 5 AGAGTAC 17790 0.0 18.564154 10-11 GTATCAA 25330 0.0 18.233433 1 ATGGGGA 2255 0.0 18.064734 5 TACTTTT 10770 0.0 17.916508 14-15 ATGGGAT 3425 0.0 17.840574 5 ACTTTTT 11355 0.0 17.809248 16-17 CCCTATA 410 3.45608E-11 17.329544 2 TGGACCG 165 0.0014919522 17.224516 5 GCACCGT 365 2.39379E-9 16.86343 6 >>END_MODULE