FastQCFastQC Report
Fri 27 May 2016
SRR939137_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939137_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887329
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122380.648429606072921No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112920.5983058597626593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95510.5060590919760147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68390.36236395456224113No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53340.28262163088682474No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42400.22465611454070808No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36760.19477261251217992No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30920.16382941183015787No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25970.1376018701561837No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25520.1352175481858224No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25040.132674271417437No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24930.13209143715801536No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24510.12986606998567818No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22900.12133549582505224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21680.1148713340387394No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20180.10692359413753512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.10544001602264363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120200.023.8463521
TACATGG122200.023.0252062
GAGTACT93300.022.8359612-13
ACATGGG122650.021.8575233
AGTACTT96400.021.63397812-13
GTACTTT98100.021.5734914-15
CCCTATA3351.8189894E-1221.2454782
GTAAGCG2050.019.6741894-95
TACCTGG13350.019.547962
CATGGGA83250.019.3782564
CATGGGG39600.018.9313664
TACTTTT109650.018.76009614-15
ACTTTTT114150.018.6428516-17
AGAGTAC187600.017.83244310-11
GTATAGG6200.017.6630651
GTATCAA257950.017.6093271
CATTCCG2704.9308437E-717.5743529
AGTACAT94500.017.4227922
ATGGGAG29200.017.224365
GTGTAGG8100.017.04681
Produced by FastQC (version 0.11.5)