##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939137_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1887329 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47497124242779 34.0 31.0 34.0 31.0 34.0 2 32.668091784739175 34.0 31.0 34.0 31.0 34.0 3 32.756488667317676 34.0 31.0 34.0 31.0 34.0 4 36.225026479220105 37.0 35.0 37.0 35.0 37.0 5 36.1921482687968 37.0 35.0 37.0 35.0 37.0 6 36.24109045110842 37.0 37.0 37.0 35.0 37.0 7 36.19023021423398 37.0 37.0 37.0 35.0 37.0 8 36.183369195301935 37.0 37.0 37.0 35.0 37.0 9 37.91091749239269 39.0 38.0 39.0 35.0 39.0 10-11 37.85213494838473 39.0 38.0 39.0 35.0 39.0 12-13 37.7918889605363 39.0 38.0 39.0 35.0 39.0 14-15 39.10777532693028 40.0 38.0 41.0 36.0 41.0 16-17 39.07050916930753 40.0 38.0 41.0 36.0 41.0 18-19 39.13343381042733 40.5 38.5 41.0 36.0 41.0 20-21 39.20849915409555 41.0 39.0 41.0 36.0 41.0 22-23 39.16566163080205 41.0 39.0 41.0 35.5 41.0 24-25 39.099929053175146 41.0 39.0 41.0 35.0 41.0 26-27 38.95536363824219 40.0 38.0 41.0 35.0 41.0 28-29 38.811304494340945 40.0 38.0 41.0 35.0 41.0 30-31 38.60570811978198 40.0 38.0 41.0 34.5 41.0 32-33 38.42867062393467 40.0 38.0 41.0 34.0 41.0 34-35 38.35251352572869 40.0 38.0 41.0 34.0 41.0 36-37 38.23230528434629 40.0 38.0 41.0 34.0 41.0 38-39 38.09828969935819 40.0 38.0 41.0 34.0 41.0 40-41 37.93266754233099 40.0 37.5 41.0 33.0 41.0 42-43 37.744861653691544 40.0 37.0 41.0 33.0 41.0 44-45 37.4749328283516 40.0 37.0 41.0 33.0 41.0 46-47 37.11109562773634 40.0 37.0 41.0 31.5 41.0 48-49 36.73200221053139 40.0 36.5 41.0 31.0 41.0 50-51 36.465713450066204 40.0 36.0 41.0 30.0 41.0 52-53 36.25022425872754 39.5 36.0 41.0 30.0 41.0 54-55 36.024453076278704 39.0 35.0 41.0 29.5 41.0 56-57 35.75545519620586 39.0 35.0 40.5 28.5 41.0 58-59 35.467357042677776 38.5 35.0 40.0 28.0 41.0 60-61 35.104343757765605 38.0 35.0 40.0 28.0 41.0 62-63 34.73939175416687 38.0 34.0 40.0 26.5 41.0 64-65 34.34239684760845 37.0 34.0 40.0 26.0 41.0 66-67 33.98511785703499 36.5 34.0 39.0 26.0 41.0 68-69 33.51964151454251 36.0 33.5 39.0 25.5 40.0 70-71 33.09565555343027 35.5 33.0 39.0 24.5 40.0 72-73 32.64261901343115 35.0 32.5 38.0 22.5 40.0 74-75 32.167130638060456 35.0 32.0 37.0 21.5 39.0 76-77 30.188921751321576 33.5 29.5 35.5 18.0 37.5 78-79 31.15233697993302 34.5 31.0 36.0 19.5 38.5 80-81 31.171004631412963 35.0 31.5 36.0 19.5 37.5 82-83 30.878847302192675 35.0 31.0 35.5 18.0 37.0 84-85 30.506801940732114 34.0 31.0 35.0 14.5 37.0 86-87 29.990168115892885 34.0 30.5 35.0 7.0 36.0 88-89 29.45642201227237 34.0 30.0 35.0 2.0 36.0 90-91 29.017536688092008 34.0 29.0 35.0 2.0 35.0 92-93 28.75597391869674 34.0 29.0 35.0 2.0 35.0 94-95 28.454886243998793 34.0 29.0 35.0 2.0 35.0 96-97 27.926350148808183 34.0 29.0 35.0 2.0 35.0 98-99 27.21959340422364 34.0 27.5 35.0 2.0 35.0 100-101 23.711854424957174 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13467141768971658 1101 2 0.059366518033506566 1101 3 0.1494137896763661 1101 4 0.16770658154197804 1101 5 0.12829101892006634 1101 6 0.1596744004863524 1101 7 0.14510969398813245 1101 8 0.13724514521630482 1101 9 0.14545956484192857 1101 10-11 0.18111098596326514 1101 12-13 0.19719661402827882 1101 14-15 0.3040920325187244 1101 16-17 0.21811052693302457 1101 18-19 0.26414316157269724 1101 20-21 0.29843372066817153 1101 22-23 0.2500112495630802 1101 24-25 0.20135202165651123 1101 26-27 0.24615061379368086 1101 28-29 0.2784486207817167 1101 30-31 0.24813299780875298 1101 32-33 0.32251738785539175 1101 34-35 0.30595129985169933 1101 36-37 0.3827912064085055 1101 38-39 0.4677428087006916 1101 40-41 0.38742416708776517 1101 42-43 0.3432839613833494 1101 44-45 0.5387119782624836 1101 46-47 0.36885466897848573 1101 48-49 0.25137742130048935 1101 50-51 0.3419876893367757 1101 52-53 0.31489379900845904 1101 54-55 0.3419042161989978 1101 56-57 0.2251825532519831 1101 58-59 0.18816575460193974 1101 60-61 0.32823716494165467 1101 62-63 0.13041169423106425 1101 64-65 0.1750173212817927 1101 66-67 0.13038863541934376 1101 68-69 0.13778043727107558 1101 70-71 0.1347269305770169 1101 72-73 0.06607722472880084 1101 74-75 -0.005156254321306619 1101 76-77 0.1533657784372977 1101 78-79 0.11120504721569091 1101 80-81 0.18822831777587012 1101 82-83 0.28278164561345775 1101 84-85 0.1768755148809973 1101 86-87 0.30857122066365505 1101 88-89 0.527980677656128 1101 90-91 0.5168340875169335 1101 92-93 0.32349964214539995 1101 94-95 0.20978026282569928 1101 96-97 0.17266389932825277 1101 98-99 0.14233616874717825 1101 100-101 0.11470069286558626 1102 1 0.16065502863080638 1102 2 0.12950551008855626 1102 3 0.1983048442293125 1102 4 0.11185978378660622 1102 5 0.1242458489781626 1102 6 0.13747132590365396 1102 7 0.11250033950788207 1102 8 0.141002308249206 1102 9 0.21041799795757044 1102 10-11 0.19024797543932692 1102 12-13 0.18766148570278318 1102 14-15 0.2369676052147156 1102 16-17 0.21774903270405588 1102 18-19 0.24978421464005152 1102 20-21 0.24305993563541506 1102 22-23 0.2837132079704432 1102 24-25 0.1860995402214698 1102 26-27 0.18764123772281494 1102 28-29 0.09755809708057228 1102 30-31 0.10895177728539096 1102 32-33 0.19379474009338793 1102 34-35 0.31962786452747594 1102 36-37 0.40202725520725124 1102 38-39 0.3756031788311631 1102 40-41 0.337869943943808 1102 42-43 0.35456258132714424 1102 44-45 0.34476388685297366 1102 46-47 0.2763594872508719 1102 48-49 0.21459949364291475 1102 50-51 0.15475526934584138 1102 52-53 0.21688211630728915 1102 54-55 0.24193036263090306 1102 56-57 0.24334818297492689 1102 58-59 0.23845782213103917 1102 60-61 0.213200787727871 1102 62-63 0.2834826124660168 1102 64-65 0.3414928416849179 1102 66-67 0.33569398414737606 1102 68-69 0.23429583098285178 1102 70-71 0.23444605451308576 1102 72-73 0.18013964735418142 1102 74-75 0.2077066017009912 1102 76-77 0.2461778866122195 1102 78-79 0.45037933497302163 1102 80-81 0.3766763487712801 1102 82-83 0.3265636477566751 1102 84-85 0.47616876890349147 1102 86-87 0.5783259394013349 1102 88-89 0.43011022530304643 1102 90-91 0.6261497442776651 1102 92-93 0.5894019218369486 1102 94-95 0.5306005477029565 1102 96-97 0.5922731290187144 1102 98-99 0.4305575973327507 1102 100-101 0.08381918176028336 1103 1 0.11759180110015421 1103 2 0.15233479857887033 1103 3 0.16354334724530162 1103 4 0.09511140470372936 1103 5 0.1199942939328551 1103 6 0.07732283201561785 1103 7 0.11688363818009861 1103 8 0.15896084664777277 1103 9 0.18652158608934855 1103 10-11 0.2567248685481971 1103 12-13 0.20380478512782219 1103 14-15 0.2973315940353274 1103 16-17 0.2266577249603543 1103 18-19 0.2715717874519257 1103 20-21 0.32954021643755027 1103 22-23 0.262673750754189 1103 24-25 0.24979852485451914 1103 26-27 0.1809834794347367 1103 28-29 0.22940335478051566 1103 30-31 0.2247431330214127 1103 32-33 0.32296466114131306 1103 34-35 0.315855291668548 1103 36-37 0.40145417043023457 1103 38-39 0.35701038897165915 1103 40-41 0.34668923873899615 1103 42-43 0.2905323555750243 1103 44-45 0.218118738312036 1103 46-47 0.232402285109103 1103 48-49 0.2987996686737304 1103 50-51 0.2080832074639858 1103 52-53 0.28746000031644314 1103 54-55 0.3199432923268475 1103 56-57 0.2966657698081008 1103 58-59 0.3484788070087461 1103 60-61 0.32874841775412733 1103 62-63 0.3512929392843205 1103 64-65 0.3023536921841341 1103 66-67 0.30949299983428347 1103 68-69 0.26705952996749005 1103 70-71 0.2770557075568263 1103 72-73 0.3861179849378118 1103 74-75 0.2515122986718268 1103 76-77 0.2679099388956203 1103 78-79 0.5284233185238882 1103 80-81 0.5798833650604358 1103 82-83 0.4313092414028681 1103 84-85 0.40835091387794265 1103 86-87 0.489113860392802 1103 88-89 0.49738699043239265 1103 90-91 0.5205478170566096 1103 92-93 0.445248986306634 1103 94-95 0.26643481422031456 1103 96-97 -0.05013420345514774 1103 98-99 -0.3177273829527536 1103 100-101 -0.517993234135524 1104 1 -0.006028056086329059 1104 2 0.06942951796921903 1104 3 0.09636282340184721 1104 4 0.034080442384976095 1104 5 0.10212369868263949 1104 6 0.06887436558842808 1104 7 0.031997947163389995 1104 8 0.02206056887403207 1104 9 0.06975517780558249 1104 10-11 0.11454434470473274 1104 12-13 0.127617156966501 1104 14-15 0.13990878168530685 1104 16-17 0.1213340312967901 1104 18-19 0.19682192924742026 1104 20-21 0.04658512561571371 1104 22-23 0.20670434222319045 1104 24-25 0.1849279950483762 1104 26-27 0.21523763513345529 1104 28-29 0.19898294931811478 1104 30-31 0.278034242807486 1104 32-33 0.0950929387987074 1104 34-35 0.18102668242783437 1104 36-37 0.22957439846647532 1104 38-39 0.2678808315190295 1104 40-41 0.27295649052417303 1104 42-43 0.17323694102316978 1104 44-45 0.15126543484059596 1104 46-47 0.29323255226635325 1104 48-49 0.12130861269723425 1104 50-51 0.07280339768809085 1104 52-53 0.01487325056003641 1104 54-55 0.2635388624901722 1104 56-57 0.27360782271598794 1104 58-59 0.19824884380286534 1104 60-61 0.19274272411965399 1104 62-63 0.2815933639436423 1104 64-65 0.19614032268689385 1104 66-67 0.20917414176066274 1104 68-69 0.15998538910275073 1104 70-71 0.19579561826202507 1104 72-73 0.010782765845590347 1104 74-75 0.16432424875981866 1104 76-77 0.2749000931982053 1104 78-79 0.43798487958321175 1104 80-81 0.4352430529649176 1104 82-83 0.3142923277454166 1104 84-85 0.2196109304571081 1104 86-87 0.3178101780728788 1104 88-89 0.33873673810912663 1104 90-91 0.46271743355433514 1104 92-93 0.3779784768608714 1104 94-95 0.20231107401874837 1104 96-97 0.20788669501927615 1104 98-99 0.21694234261842738 1104 100-101 -0.012631001183706303 1105 1 0.09889175607032286 1105 2 0.10804760675178215 1105 3 0.0883966484948786 1105 4 0.08365236777927265 1105 5 0.05500780288252827 1105 6 0.10564835548741769 1105 7 0.101578068552719 1105 8 0.11241864525184297 1105 9 0.1281073397761716 1105 10-11 0.14882354016814503 1105 12-13 0.11168342269395026 1105 14-15 0.18101197369310285 1105 16-17 0.10601084327623767 1105 18-19 0.08850880900995861 1105 20-21 0.11139301813742009 1105 22-23 0.12281584098271736 1105 24-25 0.10676284574836359 1105 26-27 0.24288665825945088 1105 28-29 0.27122815355344443 1105 30-31 0.24938148718104713 1105 32-33 0.08998013311024522 1105 34-35 0.38487160091091255 1105 36-37 0.33431838437822137 1105 38-39 0.36680455338683515 1105 40-41 0.3984867381761248 1105 42-43 0.4114538136972783 1105 44-45 0.4928072233396108 1105 46-47 0.47246318092132356 1105 48-49 0.4923975170920585 1105 50-51 0.6507788761569913 1105 52-53 0.6659536820680998 1105 54-55 0.6658777668849964 1105 56-57 0.5192440091416586 1105 58-59 0.6961015484812236 1105 60-61 0.7076851860193472 1105 62-63 0.6650011811508065 1105 64-65 0.5528280885029417 1105 66-67 0.4822821406087954 1105 68-69 0.37279467715875825 1105 70-71 0.36097952974527914 1105 72-73 0.4162851581900924 1105 74-75 0.3795106522685927 1105 76-77 0.4325525497815832 1105 78-79 0.48320545153075756 1105 80-81 0.37984255677494616 1105 82-83 0.3741646252821873 1105 84-85 0.4374665701877447 1105 86-87 0.5305696256185151 1105 88-89 0.6786102539694845 1105 90-91 0.6013848090638163 1105 92-93 0.5957219754431868 1105 94-95 0.5330706987750808 1105 96-97 0.5510233798500401 1105 98-99 0.5459520116774392 1105 100-101 0.12888282125116746 1106 1 0.04823112352766401 1106 2 0.02363359301894974 1106 3 0.1002685273962527 1106 4 -0.009505923939414629 1106 5 0.015276217364430522 1106 6 0.09269969755982999 1106 7 0.06933061744992841 1106 8 0.07722695777785304 1106 9 0.1044051966217765 1106 10-11 0.09625903746319153 1106 12-13 0.06114828432524888 1106 14-15 0.05516602591949038 1106 16-17 0.06738464685509626 1106 18-19 0.07246571183975448 1106 20-21 0.08375113819568725 1106 22-23 0.10514327023781078 1106 24-25 0.10186727250658123 1106 26-27 0.11545805339054027 1106 28-29 0.14411425946273226 1106 30-31 0.1607058733649822 1106 32-33 0.1839154483412102 1106 34-35 0.11519957635827183 1106 36-37 0.04728849985887251 1106 38-39 0.2232333663289907 1106 40-41 0.17202307296597752 1106 42-43 0.18374040857867868 1106 44-45 0.3400886163899699 1106 46-47 0.3706275847914924 1106 48-49 0.33457201251444957 1106 50-51 0.26148814581991076 1106 52-53 0.41087279359535955 1106 54-55 0.3496955207343504 1106 56-57 0.49202282728563773 1106 58-59 0.49402796858110065 1106 60-61 0.4652610027068249 1106 62-63 0.5789960573472257 1106 64-65 0.5282279792644928 1106 66-67 0.5065909473087515 1106 68-69 0.4327113178162634 1106 70-71 0.3625420297452777 1106 72-73 0.3935323492013296 1106 74-75 0.36730325526484364 1106 76-77 0.4917513377549483 1106 78-79 0.4390607626002492 1106 80-81 0.3443816745444046 1106 82-83 0.3918365457982098 1106 84-85 0.4278080703958196 1106 86-87 0.41124937489025726 1106 88-89 0.48962513120627094 1106 90-91 0.7160747799335603 1106 92-93 0.7846414250894647 1106 94-95 0.717797472599468 1106 96-97 0.7086043983685606 1106 98-99 0.6993169461343669 1106 100-101 0.37853950040222983 1107 1 0.004316130279150343 1107 2 0.008139867562761083 1107 3 0.04693862142149641 1107 4 0.028624472601840978 1107 5 0.02430524254931754 1107 6 0.10757587906943655 1107 7 0.1252143185016763 1107 8 0.13252780140359022 1107 9 0.10369969756946062 1107 10-11 0.07435388032086365 1107 12-13 0.04784321637682609 1107 14-15 0.1723030252876967 1107 16-17 0.13930002413295028 1107 18-19 0.20514567913532034 1107 20-21 0.1340329661753188 1107 22-23 0.09302280890708658 1107 24-25 0.11777321009561348 1107 26-27 0.12139812003821504 1107 28-29 0.11079228183553624 1107 30-31 0.31132001059972225 1107 32-33 0.35323831790749693 1107 34-35 0.35243803379915306 1107 36-37 0.29502427528842645 1107 38-39 0.29049765283965456 1107 40-41 0.24858365669056326 1107 42-43 0.21810329152405927 1107 44-45 0.08364807280263875 1107 46-47 0.2770281769399503 1107 48-49 0.40840412975488505 1107 50-51 0.3311640746134401 1107 52-53 0.28846468190475605 1107 54-55 0.22641993632841917 1107 56-57 0.35889905834741853 1107 58-59 0.3078242224368779 1107 60-61 0.3456262641564791 1107 62-63 0.29188117063587526 1107 64-65 0.3219339242863626 1107 66-67 0.4032514406006342 1107 68-69 0.48859316066155145 1107 70-71 0.5955354145302891 1107 72-73 0.4908441438571245 1107 74-75 0.5386605512028027 1107 76-77 0.5377084999020454 1107 78-79 0.6361858075069051 1107 80-81 0.5050084475830658 1107 82-83 0.42955875632061336 1107 84-85 0.5170450288834871 1107 86-87 0.35990403341385147 1107 88-89 0.46801697739632786 1107 90-91 0.5197308011418897 1107 92-93 0.5195069528820397 1107 94-95 0.27722910918383903 1107 96-97 0.22147650421199927 1107 98-99 0.21034756937681465 1107 100-101 0.05378788066559892 1108 1 -0.030183412008604193 1108 2 -0.0027558154943108093 1108 3 -0.0010885973578496078 1108 4 0.04479716926711319 1108 5 0.0698514596434805 1108 6 -0.023291926765836024 1108 7 0.018193142945051477 1108 8 0.06683158732926131 1108 9 0.01037376973997084 1108 10-11 0.008365748864484601 1108 12-13 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scores pass #Quality Count 2 1.0 3 6.0 4 11.0 5 40.0 6 99.0 7 200.0 8 456.0 9 843.0 10 1554.0 11 2480.0 12 3856.0 13 5606.0 14 6687.0 15 9186.0 16 12989.0 17 19837.0 18 19352.0 19 11197.0 20 9313.0 21 9687.0 22 10056.0 23 10705.0 24 12177.0 25 14493.0 26 16930.0 27 20320.0 28 24746.0 29 30055.0 30 36996.0 31 45351.0 32 56810.0 33 74356.0 34 103258.0 35 155508.0 36 249747.0 37 436573.0 38 428061.0 39 47783.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.578004333204845 19.85788151476081 15.087368574960456 21.476745577073892 2 15.15183627231924 21.8012333832628 39.922557222402666 23.124373122015292 3 19.761578399950405 26.060957045644926 30.312203118799108 23.865261435605557 4 12.752837475607063 17.332060282017604 37.693375134912884 32.22172710746245 5 13.056176215169693 38.635765147465015 33.60209057350361 14.705968063861679 6 30.17094528828837 36.92424585220701 17.550146264906648 15.354662594597974 7 27.54103815497987 34.15414058704126 20.835318060603107 17.46950319737576 8 26.04506156584252 34.36258331218352 19.86103111858081 19.731324003393155 9 26.648136069545902 17.58108946558867 21.20944467021913 34.5613297946463 10-11 24.892957189763948 27.12778217258358 26.714950069648697 21.26431056800378 12-13 26.597005609514824 24.841349865338795 27.50159087260356 21.060053652542827 14-15 23.024934158952647 25.840183689965162 27.211579652632672 23.923302498449512 16-17 21.130965864007614 29.322263489517642 27.673311381216287 21.873459265258457 18-19 21.018201123969675 27.743712872454374 30.603972758319497 20.634113245256454 20-21 22.462405865643987 26.19728727741692 30.211743686447885 21.12856317049121 22-23 21.893904030510843 26.432858288088617 30.326747482818313 21.34649019858223 24-25 22.07867040883964 26.590895965012766 30.335947803151246 20.99448582299635 26-27 21.608470475282658 26.391074909057 30.951882005512083 21.048572610148263 28-29 20.965502040184834 27.33082043459302 30.722650899763632 20.98102662545852 30-31 21.360926585607913 26.63356069842572 30.479930566484022 21.525582149482346 32-33 21.235672211893103 26.84910791918102 30.45661355280399 21.458606316121884 34-35 22.047550563607768 26.670024502842416 30.212947929340245 21.06947700420957 36-37 21.848530837687914 26.263185970580448 30.627438478720524 21.260844713011114 38-39 21.41772422917598 26.92856713208868 30.557565733252318 21.09614290548302 40-41 21.450470370188825 26.98572263195338 30.396552340855106 21.167254657002694 42-43 21.245491511071414 27.071228892270266 29.62939313546544 22.05388646119288 44-45 21.552041600852874 26.794616983588455 29.597105659023697 22.056235756534974 46-47 22.017770884922673 27.345194896096302 29.19974852971215 21.43728568926888 48-49 21.916994932227922 27.330045246734986 29.017147874086962 21.735811946950133 50-51 21.362143391155854 27.648308957589222 29.27349102725707 21.716056623997858 52-53 21.85575154702921 28.016872539840737 28.30942915562592 21.81794675750413 54-55 21.24862702793207 27.508134511788885 28.711952182157958 22.53128627812109 56-57 21.89056597975234 27.70431122501694 28.355602017454295 22.049520777776426 58-59 22.382223192478136 27.174233558909002 28.43474789051027 22.00879535810259 60-61 21.882062616545593 27.48674314583414 29.236430635401668 21.3947636022186 62-63 21.56984459667649 27.529572783263273 29.09701176393913 21.803570856121112 64-65 21.300445638630812 27.880854653348464 28.731279720170416 22.087419987850314 66-67 21.570425374870247 27.645233574936697 28.261711598815847 22.522629451377213 68-69 21.355771177019783 27.616176484614186 28.613469912433807 22.414582425932224 70-71 21.82717529036413 27.59826919373072 28.406475078397854 22.1680804375073 72-73 21.67210910233457 27.27219260658846 28.701116763425983 22.354581527650982 74-75 21.172540664611205 28.3829157502481 28.036765185084317 22.407778400056376 76-77 21.39732924670271 27.51166357089529 28.374842158140286 22.716165024261713 78-79 21.35824873365407 27.681129856092234 28.409040332323084 22.55158107793061 80-81 21.599809671365183 27.86385780034781 28.17486390336901 22.361468624918 82-83 21.563797477475948 27.60287625389707 28.364089532436537 22.469236736190442 84-85 21.27109263938614 27.708576512097256 28.112215729213087 22.908115119303524 86-87 21.237851138756 27.770057058473135 28.218748723650172 22.773343079120696 88-89 21.228380513600793 27.69446232583379 27.775540387408427 23.30161677315699 90-91 21.2811652730916 27.348629235979715 28.116873065427196 23.253332425501494 92-93 21.554773968926455 27.5830552065909 28.117196312884506 22.744974511598137 94-95 21.177362293484602 27.255608322661285 28.19556102831038 23.371468355543733 96-97 20.877864961540887 27.21102150181553 28.507218402302936 23.403895134340647 98-99 21.039675176349718 27.556494697984053 27.67891820117724 23.724911924488993 100-101 21.05087469569841 26.728230365543403 28.430146872790345 23.790748065967843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2001.0 1 1341.5 2 843.5 3 1014.5 4 886.0 5 840.0 6 1502.0 7 3242.0 8 5068.5 9 5410.0 10 4452.5 11 3610.0 12 3472.0 13 3580.5 14 3744.5 15 3756.0 16 3766.0 17 4010.0 18 4129.5 19 4233.0 20 4545.5 21 5099.5 22 5932.5 23 7040.0 24 8246.0 25 9652.0 26 11700.5 27 14150.5 28 16233.0 29 18781.5 30 22105.5 31 25758.5 32 29716.0 33 33747.5 34 37911.0 35 41108.0 36 45681.0 37 51103.0 38 53408.5 39 53885.5 40 53880.0 41 55502.0 42 57656.5 43 59902.0 44 63114.0 45 65788.5 46 66364.0 47 73796.0 48 94229.5 49 92855.5 50 74602.5 51 66158.5 52 60185.5 53 58344.5 54 56915.0 55 52172.0 56 46349.5 57 41202.0 58 37049.0 59 32898.5 60 28124.5 61 22949.5 62 18235.5 63 13874.0 64 10268.5 65 7790.5 66 5699.5 67 4412.0 68 3215.0 69 2090.0 70 1498.0 71 1068.0 72 851.0 73 751.0 74 605.0 75 483.0 76 327.5 77 173.5 78 93.5 79 57.0 80 37.0 81 19.5 82 9.5 83 7.5 84 7.0 85 3.5 86 2.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34461400211621823 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.80221254481863E-4 16-17 0.002092904840650464 18-19 2.6492466337347647E-5 20-21 0.0 22-23 0.0 24-25 6.358191920963435E-4 26-27 0.0010861911198312537 28-29 0.0 30-31 0.0029671562297829365 32-33 0.0 34-35 0.010649971467613754 36-37 0.027684627322528292 38-39 0.021379420334239552 40-41 0.013325710567685866 42-43 0.010305569405228235 44-45 0.0031790959604817174 46-47 0.001510070581228816 48-49 5.563417930843006E-4 50-51 1.0596986534939059E-4 52-53 1.0596986534939059E-4 54-55 0.0 56-57 0.0 58-59 0.004424241878337057 60-61 0.010173107073541496 62-63 0.004159317214963581 64-65 0.0019869349753010735 66-67 0.004026854883276842 68-69 0.003761930219903366 70-71 2.6492466337347647E-5 72-73 0.0 74-75 0.0 76-77 0.21803299795637113 78-79 4.768643940722577E-4 80-81 0.003549990489204585 82-83 6.888041247710389E-4 84-85 0.0 86-87 0.10967881063661926 88-89 0.40581159935549127 90-91 0.39166462233134763 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0014835781148914682 100-101 8.212664564577771E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1887329.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.28814365695782 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05046387874562 33.35082222569828 2 14.514334170939424 13.436831703790716 3 5.427662426779125 7.537092543966733 4 2.6098178872332447 4.832145011310023 5 1.4386694042063128 3.329666802838586 6 0.8924728071835406 2.4786545705304084 7 0.5620993293991045 1.8212974156093784 8 0.4053700935390885 1.5011063299177407 9 0.284974191075558 1.1871833665527698 >10 1.5882639902697042 13.730619787609575 >50 0.1461489493587425 4.677978673986578 >100 0.07330898568564193 6.237870684062382 >500 0.00332147646358799 1.0571292742114884 >1k 0.0025197407654805443 2.422437709455644 >5k 3.4360101347461966E-4 1.1517089444819646 >10k+ 2.290673423164131E-4 1.2474549559777492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12238 0.648429606072921 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11292 0.5983058597626593 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9551 0.5060590919760147 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6839 0.36236395456224113 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5334 0.28262163088682474 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4240 0.22465611454070808 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3676 0.19477261251217992 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3092 0.16382941183015787 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2597 0.1376018701561837 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2552 0.1352175481858224 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2504 0.132674271417437 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2493 0.13209143715801536 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 0.12986606998567818 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2290 0.12133549582505224 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2168 0.1148713340387394 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2018 0.10692359413753512 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.10544001602264363 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0596986534939059E-4 0.0 14-15 0.0 0.0 0.0 1.0596986534939059E-4 0.0 16-17 0.0 0.0 0.0 2.1193973069878118E-4 0.0 18-19 0.0 0.0 0.0 2.6492466337347647E-4 0.0 20-21 0.0 0.0 0.0 3.4440206238551944E-4 0.0 22-23 0.0 0.0 0.0 6.358191920963435E-4 0.0 24-25 0.0 0.0 0.0 0.0010861911198312537 0.0 26-27 0.0 0.0 0.0 0.0021723822396625073 0.0 28-29 0.0 0.0 0.0 0.005563417930843006 0.0 30-31 0.0 0.0 0.0 0.015153690744962856 0.0 32-33 0.0 0.0 0.0 0.033380507585058034 0.0 34-35 0.0 0.0 0.0 0.058627828004550346 0.0 36-37 0.0 0.0 0.0 0.10170457826907763 0.0 38-39 0.0 0.0 0.0 0.16743238725203713 0.0 40-41 0.0 0.0 0.0 0.24203517245800812 0.0 42-43 0.0 0.0 0.0 0.3134853541698347 0.0 44-45 0.0 0.0 0.0 0.4036392171158288 0.0 46-47 0.0 0.0 0.0 0.5106157961860386 0.0 48-49 0.0 0.0 0.0 0.6314744276170186 0.0 50-51 0.0 0.0 0.0 0.7671158552642385 0.0 52-53 0.0 0.0 0.0 0.9008233328688321 0.0 54-55 0.0 0.0 0.0 1.0479889833727982 0.0 56-57 0.0 0.0 0.0 1.22636275922216 0.0 58-59 0.0 0.0 0.0 1.425188719083954 0.0 60-61 0.0 0.0 0.0 1.6489440897691923 0.0 62-63 0.0 0.0 0.0 1.8716927467336113 0.0 64-65 0.0 0.0 0.0 2.1016738470081266 0.0 66-67 0.0 0.0 0.0 2.3652209021320605 0.0 68-69 0.0 0.0 0.0 2.6694338930838235 0.0 70-71 0.0 0.0 0.0 3.0066300046255847 0.0 72-73 0.0 0.0 0.0 3.348780207372429 0.0 74-75 0.0 0.0 0.0 3.7099255084831526 0.0 76-77 0.0 0.0 0.0 4.0988614067817535 0.0 78-79 0.0 0.0 0.0 4.55206802841476 0.0 80-81 0.0 0.0 0.0 5.051504003806437 0.0 82-83 0.0 0.0 0.0 5.561854875329103 0.0 84-85 0.0 0.0 0.0 6.082617286122345 0.0 86-87 0.0 0.0 0.0 6.614506532777273 0.0 88-89 0.0 0.0 0.0 7.188995665302658 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12020 0.0 23.846352 1 TACATGG 12220 0.0 23.025206 2 GAGTACT 9330 0.0 22.83596 12-13 ACATGGG 12265 0.0 21.857523 3 AGTACTT 9640 0.0 21.633978 12-13 GTACTTT 9810 0.0 21.57349 14-15 CCCTATA 335 1.8189894E-12 21.245478 2 GTAAGCG 205 0.0 19.67418 94-95 TACCTGG 1335 0.0 19.54796 2 CATGGGA 8325 0.0 19.378256 4 CATGGGG 3960 0.0 18.931366 4 TACTTTT 10965 0.0 18.760096 14-15 ACTTTTT 11415 0.0 18.64285 16-17 AGAGTAC 18760 0.0 17.832443 10-11 GTATAGG 620 0.0 17.663065 1 GTATCAA 25795 0.0 17.609327 1 CATTCCG 270 4.9308437E-7 17.574352 9 AGTACAT 9450 0.0 17.422792 2 ATGGGAG 2920 0.0 17.22436 5 GTGTAGG 810 0.0 17.0468 1 >>END_MODULE