FastQCFastQC Report
Fri 27 May 2016
SRR939136_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939136_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861292
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119160.6402004629042622No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103000.5533790506809249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90510.4862751250206846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.3384208388581695No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56550.30382121666025536No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44820.2408004762283403No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39010.2095855996802221No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT29810.16015756796891623No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25190.1353360998704126No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC23050.12383870988539143No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC22770.12233437848548213No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG21730.11674686185724754No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21620.11615587452156889No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21150.11363074681457826No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20850.1120189631718183No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20100.10798950406491835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG650.005927588329.146942
GTACATG118350.024.3544391
GAGTACT85100.023.8072612-13
TACCTGG12800.023.6818892
TACATGG121500.023.3505362
ACATGGG120650.022.5336173
GTACTTT91850.021.90336414-15
ATGGGAG26600.021.0104335
AGTACTT89000.020.9290712-13
CATGGGA78200.020.7746494
CATGGGG42300.019.7069174
ACTTTTT107450.019.16544516-17
GTATAGG5950.018.35561
ATGGGAT33350.018.1781145
TACTTTT102250.018.12464514-15
AGAGTAC177100.017.65402210-11
GTATTAG5400.017.587091
GTATCAA251050.017.2879581
GTGTAGC7800.017.0459481
AGTACAT91750.017.0354752
Produced by FastQC (version 0.11.5)