FastQCFastQC Report
Fri 27 May 2016
SRR939136_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939136_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861292
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119250.6406839979970902No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111290.5979180053425255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95120.5110428669977628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.3566339940213572No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54920.29506385886792613No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40700.21866531420110333No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34850.1872355331672838No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31520.16934473473264808No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26830.14414718378416713No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25840.13882829776305922No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25020.13442275580618193No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24570.13200508034204197No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.12819052572084336No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21000.11282485499319826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20450.10986991831480497No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19480.10465848453654775No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG3600.026.3642494-95
GAGTACT96200.023.18580812-13
GTACATG117700.023.1608581
GTACTTT99700.022.30046514-15
TACATGG121050.022.1498832
TCTATAC4850.021.5262663
GTCGGGA3750.021.5132222
AGTACTT98450.021.4267112-13
ACATGGG121500.021.0523343
ACTTTTT114100.019.5897516-17
CATGGGG38400.018.9081044
TACTTTT111450.018.82095314-15
CATGGGA82600.018.614564
AGAGTAC188450.018.2700910-11
TACCTGG13500.017.9276852
GTATCAA276900.017.6759411
CTATACT8150.017.4683324
CGGGAGT4900.017.4326824
CCTATAC4204.7293724E-1116.9484423
CCGTAAG2253.4401484E-516.9450361
Produced by FastQC (version 0.11.5)