##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939136_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1861292 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.477707420436985 34.0 31.0 34.0 31.0 34.0 2 32.680544482004976 34.0 31.0 34.0 31.0 34.0 3 32.785014925116535 34.0 31.0 34.0 31.0 34.0 4 36.24209258944862 37.0 35.0 37.0 35.0 37.0 5 36.20952918725273 37.0 37.0 37.0 35.0 37.0 6 36.26222484166912 37.0 37.0 37.0 35.0 37.0 7 36.21739898951911 37.0 37.0 37.0 35.0 37.0 8 36.19855294064553 37.0 37.0 37.0 35.0 37.0 9 37.92683361879813 39.0 38.0 39.0 35.0 39.0 10-11 37.84591133470729 39.0 38.0 39.0 35.0 39.0 12-13 37.76493183229714 39.0 38.0 39.0 35.0 39.0 14-15 39.128833896024915 40.5 38.5 41.0 36.0 41.0 16-17 39.239937366087645 41.0 39.0 41.0 36.0 41.0 18-19 39.30476437872188 41.0 39.0 41.0 36.0 41.0 20-21 39.281671548580235 41.0 39.0 41.0 36.0 41.0 22-23 39.2086612417611 41.0 39.0 41.0 36.0 41.0 24-25 39.12674072633418 41.0 39.0 41.0 35.0 41.0 26-27 38.972956956780564 40.0 38.5 41.0 35.0 41.0 28-29 38.82580245334961 40.0 38.0 41.0 35.0 41.0 30-31 38.62321414372382 40.0 38.0 41.0 35.0 41.0 32-33 38.483479754922925 40.0 38.0 41.0 34.0 41.0 34-35 38.354649888357116 40.0 38.0 41.0 34.0 41.0 36-37 38.19937092084423 40.0 38.0 41.0 34.0 41.0 38-39 38.062277170911386 40.0 38.0 41.0 34.0 41.0 40-41 37.8812271261038 40.0 37.5 41.0 33.0 41.0 42-43 37.66836342712482 40.0 37.0 41.0 33.0 41.0 44-45 37.36319610249225 40.0 37.0 41.0 32.5 41.0 46-47 36.96907121504847 40.0 37.0 41.0 31.5 41.0 48-49 36.55701442868717 40.0 36.0 41.0 30.5 41.0 50-51 36.27897342276225 39.5 36.0 41.0 30.0 41.0 52-53 36.019556039568215 39.0 35.0 41.0 29.5 41.0 54-55 35.79165816003078 39.0 35.0 40.0 29.0 41.0 56-57 35.55347334002403 39.0 35.0 40.0 28.0 41.0 58-59 35.22773455212831 38.0 35.0 40.0 27.5 41.0 60-61 34.84125462313275 38.0 34.0 40.0 26.5 41.0 62-63 34.45776750773119 37.5 34.0 40.0 26.0 41.0 64-65 34.01579816600511 37.0 34.0 39.5 25.5 41.0 66-67 33.59499557296759 36.0 33.0 39.0 24.5 40.5 68-69 33.10182496889257 36.0 33.0 39.0 22.5 40.0 70-71 32.58901021441021 35.0 32.0 38.0 22.0 40.0 72-73 32.01872597099219 35.0 31.5 37.5 20.5 39.5 74-75 31.489855971013682 35.0 31.0 37.0 18.5 39.0 76-77 29.497808242876452 32.5 28.5 35.0 13.0 37.0 78-79 30.4758458103296 34.0 30.5 36.0 13.5 38.0 80-81 30.483621054622276 34.0 31.0 35.5 12.0 37.0 82-83 30.098823827749754 34.0 30.0 35.0 7.0 37.0 84-85 29.627242259677686 34.0 30.0 35.0 3.5 36.0 86-87 28.953029938343903 34.0 29.0 35.0 2.0 36.0 88-89 28.28572840800906 33.5 28.5 35.0 2.0 35.0 90-91 27.7261246488998 33.0 27.0 35.0 2.0 35.0 92-93 27.285307195217086 33.0 26.0 35.0 2.0 35.0 94-95 26.765462646376818 32.5 25.5 34.0 2.0 35.0 96-97 25.970384012825498 32.0 24.5 34.0 2.0 35.0 98-99 24.809508126613125 32.0 11.0 34.0 2.0 35.0 100-101 19.023691339134324 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1246239863459877 1101 2 0.15653484811065255 1101 3 0.20391011673504522 1101 4 0.17424168977501608 1101 5 0.1898619788759035 1101 6 0.1325038640851588 1101 7 0.18863416154917445 1101 8 0.22435125261149125 1101 9 0.30269832135466146 1101 10-11 0.1978682681123516 1101 12-13 0.24462864170418896 1101 14-15 0.34941924221779175 1101 16-17 0.30496838337653287 1101 18-19 0.2906978826418296 1101 20-21 0.2617426251461552 1101 22-23 0.2661113938658133 1101 24-25 0.2868772120415599 1101 26-27 0.38425348227536915 1101 28-29 0.2910526430602616 1101 30-31 0.38394906907319637 1101 32-33 0.443515678207838 1101 34-35 0.5138002351343118 1101 36-37 0.5481189787379392 1101 38-39 0.5042702649298292 1101 40-41 0.5235255318272394 1101 42-43 0.597985138744157 1101 44-45 0.571183776590793 1101 46-47 0.6128874046167994 1101 48-49 0.7174142409647644 1101 50-51 0.6713844537607585 1101 52-53 0.8285756086864424 1101 54-55 0.7709216000625787 1101 56-57 0.6099747886008515 1101 58-59 0.5284444349069872 1101 60-61 0.5608054106616933 1101 62-63 0.5165221909177617 1101 64-65 0.5577655956223353 1101 66-67 0.5943083163168055 1101 68-69 0.7734060630351962 1101 70-71 0.7313601433906243 1101 72-73 0.5711592988603442 1101 74-75 0.5416872216798545 1101 76-77 0.5665073192638843 1101 78-79 0.6537006298476982 1101 80-81 0.6747449349662276 1101 82-83 0.5567969443843701 1101 84-85 0.6808599267652156 1101 86-87 0.8029419028738651 1101 88-89 0.8046092404795004 1101 90-91 0.8092744791683337 1101 92-93 0.626881807139938 1101 94-95 0.6141618668239239 1101 96-97 0.5517331505966254 1101 98-99 0.7249108753919238 1101 100-101 0.16677682890767187 1102 1 0.10754128587984724 1102 2 0.06827632404423412 1102 3 0.11869145020804694 1102 4 0.07836361927797952 1102 5 0.09426882955416716 1102 6 0.0875743087507388 1102 7 0.14715299043934493 1102 8 0.0898663628613221 1102 9 0.19022568740504653 1102 10-11 0.16220912601838933 1102 12-13 0.19286621568418383 1102 14-15 0.26959836545667315 1102 16-17 0.2401184383705086 1102 18-19 0.22785286090611123 1102 20-21 0.15481233655872018 1102 22-23 0.1289836507138773 1102 24-25 0.20498227672494806 1102 26-27 0.12069930011436725 1102 28-29 0.16116734478340078 1102 30-31 0.1017000772961083 1102 32-33 0.1809419600912534 1102 34-35 0.17850457703971756 1102 36-37 0.22824153674348935 1102 38-39 0.14416446699341634 1102 40-41 0.0016106939285336352 1102 42-43 0.0642125519322363 1102 44-45 0.1246871547922268 1102 46-47 0.18139418720728173 1102 48-49 0.011512601620502494 1102 50-51 -0.13686464276676702 1102 52-53 -0.14564949999212473 1102 54-55 -0.14152275127037228 1102 56-57 -0.004033486683283627 1102 58-59 -0.10469964931236575 1102 60-61 -0.19437083194031146 1102 62-63 -0.2402619708168956 1102 64-65 0.042943409480820094 1102 66-67 -0.14651816459242895 1102 68-69 -0.08717111184564885 1102 70-71 -0.0987514158195566 1102 72-73 -0.08192507761270917 1102 74-75 -0.11980966199479681 1102 76-77 -0.005554692991914578 1102 78-79 -0.10485276668506316 1102 80-81 0.03170612493114078 1102 82-83 -0.09486073120353922 1102 84-85 -0.1292841050789164 1102 86-87 0.056407570919748906 1102 88-89 0.30731180162756644 1102 90-91 0.3578870596952264 1102 92-93 0.19308303271998994 1102 94-95 0.27982153267004506 1102 96-97 0.24831095398006298 1102 98-99 0.11451963252804731 1102 100-101 -0.4250463523629513 1103 1 0.0769767223479576 1103 2 0.10504712780453218 1103 3 0.1984844822328995 1103 4 0.12723621161584475 1103 5 0.17090016185052548 1103 6 0.21775140712050955 1103 7 0.136772202953388 1103 8 0.13806798869553205 1103 9 0.1535832389770988 1103 10-11 0.16994463406158644 1103 12-13 0.1462228475762828 1103 14-15 0.15682481065374532 1103 16-17 0.2555584652411582 1103 18-19 0.3029909128640327 1103 20-21 0.359869176778318 1103 22-23 0.331385814760786 1103 24-25 0.38966840595197993 1103 26-27 0.28868484114608606 1103 28-29 0.3032359049244704 1103 30-31 0.33812560121378255 1103 32-33 0.28443034613290763 1103 34-35 0.3071536071772343 1103 36-37 0.3273602434335672 1103 38-39 0.4016922473258546 1103 40-41 0.3996337818939182 1103 42-43 0.34420151577281644 1103 44-45 0.3056955638658607 1103 46-47 0.4741289155218311 1103 48-49 0.5432397605691932 1103 50-51 0.5209320439862424 1103 52-53 0.45357017156216983 1103 54-55 0.4108824117328993 1103 56-57 0.46100927843446016 1103 58-59 0.3618291986485431 1103 60-61 0.5140812267432722 1103 62-63 0.4988938029738179 1103 64-65 0.41842954030729373 1103 66-67 0.2946725010559277 1103 68-69 0.337648251016077 1103 70-71 0.3726133492422079 1103 72-73 0.37006223080395984 1103 74-75 0.2190384188730654 1103 76-77 0.055623017164950994 1103 78-79 0.09574642479630313 1103 80-81 0.243370265810114 1103 82-83 0.07813406660682176 1103 84-85 -0.14443583227784984 1103 86-87 -0.23517315898624602 1103 88-89 -0.08683703457743519 1103 90-91 0.07857585999272132 1103 92-93 0.10165744882318961 1103 94-95 -0.1832679122031422 1103 96-97 -0.5834587901489776 1103 98-99 -0.562810456600932 1103 100-101 -0.8085804824856666 1104 1 0.0709692129612236 1104 2 0.10218170391940617 1104 3 0.10866167741108512 1104 4 0.06256326516962929 1104 5 0.08809270516124457 1104 6 0.011322318127689357 1104 7 0.03812916231048291 1104 8 0.07184046745187089 1104 9 0.1388938245775151 1104 10-11 0.187456655667539 1104 12-13 0.09392062769617127 1104 14-15 0.001156541106283271 1104 16-17 0.07913069368460413 1104 18-19 0.20777650011124393 1104 20-21 0.22248362905419583 1104 22-23 0.196924975553884 1104 24-25 0.11303972536440199 1104 26-27 0.13608619810545264 1104 28-29 0.14121759256033073 1104 30-31 0.1584108261660262 1104 32-33 0.08231586092113474 1104 34-35 0.1364566833909251 1104 36-37 0.2923954848537633 1104 38-39 0.29643897020314114 1104 40-41 0.28170347427255393 1104 42-43 0.2352627106875147 1104 44-45 0.1735204765857148 1104 46-47 -0.004617543868207008 1104 48-49 -0.2389517946643238 1104 50-51 -0.12160138595908165 1104 52-53 -0.27637186124199076 1104 54-55 -0.2949110142601157 1104 56-57 -0.24928319241726626 1104 58-59 -0.304424671320497 1104 60-61 -0.08194340931495958 1104 62-63 -0.161388787105885 1104 64-65 -0.15427318019860792 1104 66-67 -0.162586572786374 1104 68-69 -0.3071248205865942 1104 70-71 -0.12763696364890365 1104 72-73 -0.2828954136199897 1104 74-75 -0.45586442020209716 1104 76-77 -0.4387548235570051 1104 78-79 -0.2496623098061619 1104 80-81 -0.005239838926073048 1104 82-83 -0.045385464385866214 1104 84-85 -0.43289838415190474 1104 86-87 -0.22576337043476613 1104 88-89 -0.05668091837955558 1104 90-91 0.06758844150909127 1104 92-93 0.07253881368716364 1104 94-95 0.1772913394930562 1104 96-97 -0.275929641871528 1104 98-99 -0.500531950573663 1104 100-101 -0.6595125686460008 1105 1 0.10175562005075278 1105 2 0.10591119721513564 1105 3 0.0867819834094874 1105 4 0.06948311870759483 1105 5 -0.06804323863376993 1105 6 0.03898851103301837 1105 7 0.11503091008582089 1105 8 0.10079720092255684 1105 9 0.2337011799976807 1105 10-11 0.1502428864737837 1105 12-13 0.14375646388491958 1105 14-15 0.11561783039817186 1105 16-17 0.16378486627328925 1105 18-19 0.19933895764717136 1105 20-21 0.24041714821949256 1105 22-23 0.2109973398432956 1105 24-25 0.21326871000938752 1105 26-27 0.3212429616911976 1105 28-29 0.3214641320826743 1105 30-31 0.3288427936711429 1105 32-33 0.16969618109565232 1105 34-35 0.25291988976356095 1105 36-37 0.27041406070515706 1105 38-39 0.37920268816456826 1105 40-41 0.4160926710450994 1105 42-43 0.4546131067740262 1105 44-45 0.43800582120118037 1105 46-47 0.48342879660093274 1105 48-49 0.3824497309206265 1105 50-51 0.3453912211605612 1105 52-53 0.38105737754474234 1105 54-55 0.28190112284421787 1105 56-57 0.1391103894020489 1105 58-59 0.08048107220935918 1105 60-61 0.06683325812294783 1105 62-63 0.11024815011889899 1105 64-65 0.19128172653652342 1105 66-67 0.24894437411195725 1105 68-69 -0.005127861473297912 1105 70-71 -0.021517362783647798 1105 72-73 0.030279925817154663 1105 74-75 0.08852793497934641 1105 76-77 0.07350457730017723 1105 78-79 -0.0015248529129365807 1105 80-81 -0.041518021298475816 1105 82-83 0.09887247478905792 1105 84-85 0.043939093674964624 1105 86-87 -0.06990837812879747 1105 88-89 -0.12079184463805248 1105 90-91 0.352404207111082 1105 92-93 0.2878677472834532 1105 94-95 0.1489029359953058 1105 96-97 0.14276556799739737 1105 98-99 0.21117674863930702 1105 100-101 -0.7107843791120843 1106 1 -0.0829385473259876 1106 2 -0.09094610317080765 1106 3 -0.07874438953756169 1106 4 -0.027809414665831866 1106 5 -0.016299800543784215 1106 6 -0.002477664430195148 1106 7 0.015096487416947468 1106 8 -0.014600105812277775 1106 9 0.024624358451553974 1106 10-11 -0.07006561936552913 1106 12-13 -0.021069234075078214 1106 14-15 -0.06453500237545029 1106 16-17 -0.10693360230806803 1106 18-19 -0.1492755645989945 1106 20-21 -0.07818425856045508 1106 22-23 -0.0874505240900092 1106 24-25 -0.10370579419257098 1106 26-27 0.012074039498493505 1106 28-29 0.016215230607393494 1106 30-31 -0.07054508162332951 1106 32-33 -0.018178695685797663 1106 34-35 -0.1014733737319986 1106 36-37 -0.12101786417746752 1106 38-39 -0.019317436139978383 1106 40-41 -0.2787868671568319 1106 42-43 -0.35523351745533915 1106 44-45 -0.26155288930623755 1106 46-47 -0.3635922382678274 1106 48-49 -0.4353183842949804 1106 50-51 -0.4332863305076913 1106 52-53 -0.22342777775239853 1106 54-55 -0.36672655663718956 1106 56-57 -0.35024686921489234 1106 58-59 -0.4110408648698183 1106 60-61 -0.11533597316037003 1106 62-63 -0.02728255009662206 1106 64-65 -0.06123694420680437 1106 66-67 -0.16100896991424207 1106 68-69 -0.35729796990561624 1106 70-71 -0.3512147046044447 1106 72-73 -0.3158608273614689 1106 74-75 -0.35387365725496167 1106 76-77 -0.27703862855416617 1106 78-79 -0.2484099296843567 1106 80-81 -0.2138433433374729 1106 82-83 -0.13561242989408484 1106 84-85 -0.283102783925159 1106 86-87 -0.3425377784930248 1106 88-89 -0.2973995334757049 1106 90-91 0.10531446223613727 1106 92-93 0.14975123683284863 1106 94-95 -0.062267556711827154 1106 96-97 -0.4064560666742132 1106 98-99 -0.4781840603427021 1106 100-101 -1.0393027908876036 1107 1 -0.1169661311480894 1107 2 -0.04445526009536138 1107 3 -0.10668664172870734 1107 4 -0.12564220935654902 1107 5 -0.011622254626161066 1107 6 -0.03740464843051683 1107 7 -0.007687762938822118 1107 8 -0.0406986707619339 1107 9 0.023311333939993517 1107 10-11 0.04498478694390684 1107 12-13 0.08681040922380845 1107 14-15 0.0737716315736634 1107 16-17 -0.04797268382264974 1107 18-19 -0.06715750211645144 1107 20-21 -0.011671204789287515 1107 22-23 -0.08778634448926681 1107 24-25 -0.01736836451535595 1107 26-27 -0.03743137352734749 1107 28-29 -0.05915866052029628 1107 30-31 0.06960457457638114 1107 32-33 0.07492235632978606 1107 34-35 0.029422767990858745 1107 36-37 0.10015910319807375 1107 38-39 0.16920315862788016 1107 40-41 0.151412031618257 1107 42-43 0.1032969856767707 1107 44-45 0.14477965268793724 1107 46-47 0.1940651318261999 1107 48-49 0.19196585941087818 1107 50-51 0.16234251812708322 1107 52-53 0.15691908149562295 1107 54-55 0.13899386124309387 1107 56-57 0.140831613009901 1107 58-59 0.04701500101481315 1107 60-61 0.08029270922668275 1107 62-63 0.11414072990444168 1107 64-65 -0.01287481676794755 1107 66-67 0.07087451703198866 1107 68-69 0.20806913831499685 1107 70-71 0.1459507717285291 1107 72-73 -0.1203207550104608 1107 74-75 -0.07698458438526146 1107 76-77 -0.08797556746810642 1107 78-79 0.23199162506434234 1107 80-81 0.2304761382356304 1107 82-83 0.05732636434443705 1107 84-85 0.009328788512302566 1107 86-87 -0.022246412734734378 1107 88-89 -0.1999929979452446 1107 90-91 0.014641125710848968 1107 92-93 0.3008928599426106 1107 94-95 0.24874785112708153 1107 96-97 0.12361166264789603 1107 98-99 0.21382092043906198 1107 100-101 -0.5526110873139416 1108 1 -0.007755360980503667 1108 2 0.08461855470497426 1108 3 0.025405984578121377 1108 4 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-0.7685658170606793 2316 100-101 0.15884237595698636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 9.0 5 39.0 6 94.0 7 234.0 8 463.0 9 885.0 10 1501.0 11 2599.0 12 3904.0 13 5634.0 14 6894.0 15 9584.0 16 13172.0 17 20511.0 18 20289.0 19 12353.0 20 10728.0 21 11121.0 22 11613.0 23 12668.0 24 14238.0 25 16529.0 26 19368.0 27 22898.0 28 27870.0 29 33970.0 30 41280.0 31 50919.0 32 64673.0 33 84553.0 34 119482.0 35 179771.0 36 286494.0 37 444521.0 38 295877.0 39 14546.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64406551036859 19.758916922864422 15.110991450242961 21.486026116524027 2 15.213786982375682 21.729744714961434 39.89073181424516 23.165736488417725 3 19.741717043859857 26.01741156143152 30.36396223698377 23.87690915772485 4 12.644120320723454 17.374973942831108 37.75012195829564 32.2307837781498 5 13.039490848292475 38.6197866858075 33.58376869400395 14.75695377189608 6 30.193704158186897 36.87105515953434 17.526696509736247 15.408544172542513 7 27.513146781912777 34.226118201765225 20.822847785301825 17.437887231020174 8 26.087470423770153 34.23525164240753 19.92481566567739 19.752462268144924 9 26.711015789032565 17.5393221482712 21.160570184581463 34.58909187811477 10-11 24.915488810997953 27.13204053958218 26.684313906684174 21.26815674273569 12-13 26.61737115938821 24.834228052342137 27.498989948917202 21.04941083935245 14-15 23.071858307804224 25.813125848209346 27.230837587587857 23.884178256398574 16-17 21.112260275996572 29.36284926031092 27.69625870716264 21.828631756529866 18-19 21.034614943441156 27.74087239962145 30.562595917941927 20.66191673899548 20-21 22.489915607008466 26.166850768176086 30.248397349797884 21.094836275017567 22-23 21.893851152855113 26.349734485507913 30.373041951504653 21.383372410132317 24-25 22.092488493201763 26.558286694557705 30.39793347735523 20.9512913348853 26-27 21.58827824500342 26.389798535694002 30.976782470450882 21.045140748851697 28-29 20.96782902391057 27.30375903372366 30.74357857818464 20.984833364181124 30-31 21.34683864756309 26.581670151701076 30.496531441600784 21.57495975913505 32-33 21.21783246623781 26.881456709739826 30.47630125442536 21.424409569597007 34-35 22.007596701039347 26.626066388654706 30.23522477111298 21.13111213919297 36-37 21.896219786302453 26.202549162124505 30.69398634732502 21.207244704248023 38-39 21.403476435694216 26.908253888246144 30.579592235920877 21.10867744013877 40-41 21.510948461928216 26.911433866217767 30.352006765785717 21.225610906068308 42-43 21.296571667597554 26.99979340436032 29.660363753399494 22.04327117464263 44-45 21.549233192780665 26.776357923602962 29.63582029890008 22.038588584716294 46-47 22.086544209006348 27.33413479297492 29.150528896202022 21.428792101816715 48-49 21.93233021505408 27.284168878690135 28.990917025546825 21.792583880708964 50-51 21.329979363630454 27.657731761580894 29.263296113367325 21.748992761421327 52-53 21.904139705108065 27.997917575533553 28.28610449085904 21.811838228499344 54-55 21.234819684391272 27.438870419042257 28.78769156048594 22.53861833608053 56-57 21.86884164333162 27.663230702114443 28.3946043930775 22.07332326147644 58-59 22.407326866565356 27.112366652796133 28.472601483028026 22.00770499761049 60-61 21.881563970870108 27.439335779539498 29.293090474973937 21.38600977461646 62-63 21.60397450334826 27.493225034170326 29.119995873771803 21.78280458870961 64-65 21.23374334717 27.873598110504943 28.774074030295964 22.11858451202909 66-67 21.553577026739152 27.629194102165766 28.27652457918952 22.540704291905563 68-69 21.31081745428365 27.5589470262545 28.65592452201665 22.474310997445205 70-71 21.802083501227642 27.602200624298202 28.377147032434497 22.21856884203966 72-73 21.66288255684761 27.24282380196122 28.70597412979801 22.388319511393163 74-75 21.166103975088273 28.358178082751124 28.047694827034125 22.428023115126482 76-77 21.362964014624428 27.524836763168853 28.34422744067397 22.767971781532747 78-79 21.425796394482266 27.63883672106271 28.36616816614463 22.569198718310396 80-81 21.62981488595245 27.851752955619368 28.12081438361132 22.39761777481686 82-83 21.554114024022024 27.576086933162557 28.33338347771333 22.536415565102093 84-85 21.228291960638092 27.69844817470875 28.1748914195086 22.898368445144555 86-87 21.239262180285653 27.703789995890958 28.207012686385745 22.849935137437637 88-89 21.20446119517648 27.61177804728569 27.80335285587273 23.380407901665098 90-91 21.26528161221245 27.26446260034649 28.093860486346294 23.376395301094767 92-93 21.57756547602418 27.513146781912777 28.020939218564312 22.88834852349873 94-95 21.177440186709017 27.19798935363178 28.158182595745323 23.466387863913884 96-97 20.815997704820095 27.16465229528736 28.50630099952076 23.513049000371787 98-99 21.033530508410834 27.448624341182725 27.684643446212036 23.833201704194405 100-101 21.3221127757905 26.195033971136084 28.512813184002887 23.970040069070524 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1861.0 1 1268.0 2 848.0 3 1044.0 4 875.5 5 810.5 6 1447.0 7 3104.0 8 4759.5 9 5147.5 10 4363.5 11 3480.5 12 3336.0 13 3530.0 14 3734.5 15 3764.0 16 3759.0 17 3880.0 18 3997.0 19 4195.0 20 4500.0 21 5007.5 22 5888.0 23 6971.5 24 8143.0 25 9590.5 26 11597.5 27 13930.0 28 16018.5 29 18517.0 30 21813.0 31 25552.0 32 29356.0 33 33232.5 34 37544.5 35 40790.0 36 45031.5 37 50203.0 38 52275.5 39 52589.5 40 52864.5 41 54630.5 42 56501.5 43 58614.5 44 62434.5 45 65376.5 46 65727.0 47 72804.0 48 92295.0 49 91292.0 50 73800.0 51 65535.5 52 59496.0 53 57298.5 54 55823.0 55 51458.5 56 46522.0 57 41399.0 58 36904.5 59 32714.5 60 27755.0 61 22655.5 62 18044.5 63 13609.5 64 10097.5 65 7630.0 66 5510.5 67 4303.0 68 3166.0 69 2095.0 70 1509.5 71 1128.5 72 861.5 73 721.0 74 605.0 75 491.0 76 335.5 77 176.0 78 113.5 79 67.5 80 38.0 81 24.5 82 18.0 83 11.5 84 3.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42475871598867887 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.670701856559852E-4 16-17 0.001987866492737303 18-19 2.686306071266626E-5 20-21 0.0 22-23 0.0 24-25 5.372612142533252E-5 26-27 0.0037071023783479436 28-29 1.3431530356333127E-4 30-31 0.0 32-33 8.058918213799876E-5 34-35 0.011873472834998485 36-37 0.026030305830573603 38-39 0.019502582077395702 40-41 0.008757357792329199 42-43 0.009052851460168527 44-45 0.0036533762569226106 46-47 0.005614379688947247 48-49 0.0033578825890832817 50-51 8.058918213799877E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0017460989463233068 60-61 0.0022833601605766318 62-63 0.0015043313999093103 64-65 0.0029012105569679557 66-67 0.0017460989463233068 68-69 0.0046741725640039285 70-71 1.0745224285066503E-4 72-73 0.0 74-75 0.0 76-77 0.09673388162631119 78-79 0.01670882376327841 80-81 3.760828499773276E-4 82-83 0.0 84-85 0.0 86-87 0.10616281593645704 88-89 0.41412094394646304 90-91 0.4211590658531815 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.0745224285066503E-4 100-101 3.2235672855199503E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1861292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.63440877264325 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34157624852008 33.73606638030826 2 14.542506004665464 13.563623392003766 3 5.301949545398394 7.41759847176116 4 2.570471931200887 4.7948975511291145 5 1.3230626705181647 3.0850122704384546 6 0.8589868526008473 2.4035006408708504 7 0.5834287227366328 1.904549748206089 8 0.38858228803916967 1.4497044209782113 9 0.2888997539712993 1.2125402298912271 >10 1.5777161811880067 13.72501867468727 >50 0.14327895651391998 4.611767207957166 >100 0.07320119265934313 6.232202312277138 >500 0.0031121927939610477 1.0024009379095793 >1k 0.0026511271948557067 2.4585377357985 >5k 3.457991993290052E-4 1.1633398272328999 >10k+ 2.3053279955267017E-4 1.2392401985503776 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11925 0.6406839979970902 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11129 0.5979180053425255 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9512 0.5110428669977628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6638 0.3566339940213572 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5492 0.29506385886792613 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4070 0.21866531420110333 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3485 0.1872355331672838 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3152 0.16934473473264808 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2683 0.14414718378416713 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2584 0.13882829776305922 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2502 0.13442275580618193 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2457 0.13200508034204197 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.12819052572084336 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2100 0.11282485499319826 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2045 0.10986991831480497 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1948 0.10465848453654775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.372612142533251E-5 0.0 14-15 0.0 0.0 0.0 5.372612142533251E-5 0.0 16-17 0.0 0.0 0.0 1.3431530356333127E-4 0.0 18-19 0.0 0.0 0.0 2.1490448570133004E-4 0.0 20-21 0.0 0.0 0.0 3.760828499773276E-4 0.0 22-23 0.0 0.0 0.0 8.864810035179864E-4 0.0 24-25 0.0 0.0 0.0 0.0012357007927826479 0.0 26-27 0.0 0.0 0.0 0.002122181796300634 0.0 28-29 0.0 0.0 0.0 0.005963599478211908 0.0 30-31 0.0 0.0 0.0 0.015473122970495762 0.0 32-33 0.0 0.0 0.0 0.0329609754944415 0.0 34-35 0.0 0.0 0.0 0.05813166338220978 0.0 36-37 0.0 0.0 0.0 0.10044098400465913 0.0 38-39 0.0 0.0 0.0 0.1674105943613361 0.0 40-41 0.0 0.0 0.0 0.24125714826045563 0.0 42-43 0.0 0.0 0.0 0.3164199921344958 0.0 44-45 0.0 0.0 0.0 0.4089363732289184 0.0 46-47 0.0 0.0 0.0 0.521573186797128 0.0 48-49 0.0 0.0 0.0 0.6412749853327688 0.0 50-51 0.0 0.0 0.0 0.7800226939136901 0.0 52-53 0.0 0.0 0.0 0.9153050676626773 0.0 54-55 0.0 0.0 0.0 1.0629981754609164 0.0 56-57 0.0 0.0 0.0 1.2411271310466065 0.0 58-59 0.0 0.0 0.0 1.4339233177814121 0.0 60-61 0.0 0.0 0.0 1.6608624546820165 0.0 62-63 0.0 0.0 0.0 1.8921802704787858 0.0 64-65 0.0 0.0 0.0 2.1257008572539933 0.0 66-67 0.0 0.0 0.0 2.399247404491074 0.0 68-69 0.0 0.0 0.0 2.7101336061187604 0.0 70-71 0.0 0.0 0.0 3.059810067415537 0.0 72-73 0.0 0.0 0.0 3.411689299690752 0.0 74-75 0.0 0.0 0.0 3.7734004121868034 0.0 76-77 0.0 0.0 0.0 4.154023119424572 0.0 78-79 0.0 0.0 0.0 4.605644896125916 0.0 80-81 0.0 5.372612142533251E-5 0.0 5.102745834613806 0.0 82-83 0.0 5.372612142533251E-5 0.0 5.608120595801196 0.0 84-85 0.0 5.372612142533251E-5 0.0 6.12663139367708 0.0 86-87 0.0 5.372612142533251E-5 0.0 6.656558992355848 0.0 88-89 0.0 5.372612142533251E-5 0.0 7.238278572088635 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 360 0.0 26.36424 94-95 GAGTACT 9620 0.0 23.185808 12-13 GTACATG 11770 0.0 23.160858 1 GTACTTT 9970 0.0 22.300465 14-15 TACATGG 12105 0.0 22.149883 2 TCTATAC 485 0.0 21.526266 3 GTCGGGA 375 0.0 21.513222 2 AGTACTT 9845 0.0 21.42671 12-13 ACATGGG 12150 0.0 21.052334 3 ACTTTTT 11410 0.0 19.58975 16-17 CATGGGG 3840 0.0 18.908104 4 TACTTTT 11145 0.0 18.820953 14-15 CATGGGA 8260 0.0 18.61456 4 AGAGTAC 18845 0.0 18.27009 10-11 TACCTGG 1350 0.0 17.927685 2 GTATCAA 27690 0.0 17.675941 1 CTATACT 815 0.0 17.468332 4 CGGGAGT 490 0.0 17.432682 4 CCTATAC 420 4.7293724E-11 16.948442 3 CCGTAAG 225 3.4401484E-5 16.945036 1 >>END_MODULE